{"id":17430,"date":"2018-08-08T17:04:50","date_gmt":"2018-08-08T09:04:50","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=17430"},"modified":"2024-05-26T17:52:43","modified_gmt":"2024-05-26T09:52:43","slug":"ap%e9%85%b8%e6%80%a7%e7%a3%b7%e9%85%b8%e9%85%b6%e6%aa%a2%e6%b8%ac-acid-phosphatase-activity-colorimetric-assay-kit%e8%b2%a8%e8%99%9fk411-500","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=17430","title":{"rendered":"AP\u9178\u6027\u78f7\u9178\u9176\u6aa2\u6e2c | Acid Phosphatase Activity Colorimetric Assay Kit\u8ca8\u865fK411-500 (ABCAM ab83367)"},"content":{"rendered":"<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=17430\">\u9178\u6027\u78f7\u9178\u9176\u6aa2\u6e2c\u8a66\u5291\u76d2 <strong>| <\/strong><strong>\u8ca8\u865f<\/strong>K411-500<\/a>\u00a0(ABCAM ab83367)\u00a0<\/span><\/p>\n<p><strong><em>Acid Phosphatase Activity Colorimetric Assay Kit<\/em><\/strong><\/p>\n<p><strong>\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong>\u9178\u6027\u78f7\u9178\u9176Acid phosphatases\uff08AP\uff09\u5728\u9178\u6027\u689d\u4ef6\u4e0b\u4f7f\u78f7\u9178\u916f\u57fa\u5718\u7684\u78f7\u9178\u916f\u57fa\u5718\u53bb\u78f7\u9178\u5316\u3002 \u5728\u4e0d\u540c\u5668\u5b98\u4e2d\u767c\u73fe\u4e0d\u540c\u7684acid phosphatase isozymes\u9178\u6027\u78f7\u9178\u9176\u540c\u5de5\u9176\uff0c\u5176\u8840\u6e05\u6c34\u5e73\u7528\u70ba\u76f8\u61c9\u5668\u5b98\u4e2d\u75be\u75c5\u7684\u8a3a\u65b7\u3002 \u5347\u9ad8\u7684\u524d\u5217\u817a\u9178\u6027\u78f7\u9178\u9176\u6c34\u5e73\u53ef\u80fd\u8868\u660e\u5b58\u5728\u524d\u5217\u817a\u764c\uff0c\u4e26\u4e14Tartrate-Resistant Acid Phosphatase\u9152\u77f3\u9178\u6297\u6027\u9178\u6027\u78f7\u9178\u9176\u6c34\u5e73\u5347\u9ad8\u53ef\u80fd\u8868\u660ebone disease\u9aa8\u75c5\u3002 BioVision\u7684\u9178\u6027\u78f7\u9178\u9176\u6aa2\u6e2c\u8a66\u5291\u76d2\u662f\u4e00\u7a2e\u9ad8\u9748\u654f\u5ea6\uff0c\u7c21\u55ae\uff0c\u76f4\u63a5\u548cHTS\u5c31\u7dd2\u7684\u6bd4\u8272\u6aa2\u6e2c\u65b9\u6cd5\uff0c\u7528\u65bc\u6e2c\u91cf\u8840\u6e05\u548c\u5176\u4ed6\u6a23\u54c1\u4e2d\u7684AP\u6d3b\u6027\u3002 \u5b83\u9069\u7528\u65bcresearch\u7814\u7a76\u548cdrug discovery\u85e5\u7269\u767c\u73fe\u3002 \u8a72\u8a66\u5291\u76d2\u4f7f\u7528\u78f7\u9178\u5c0d\u785d\u57fa\u82ef\u916f\uff08pNPP\uff09\u4f5c\u70ba\u78f7\u9178\u9176\u5e95\u7269\uff0c\u7576AP\u53bb\u78f7\u9178\u5316\u6642\uff0c\u5176\u8b8a\u9ec3\uff08\u03bbmax= 405nm\uff09\u3002 \u8a72\u8a66\u5291\u76d2\u53ef\u4ee5\u6aa2\u6e2c\u6a23\u54c1\u4e2d\u7684\u03bcU\u9178\u6027\u78f7\u9178\u9176\u6d3b\u6027\u3002<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/www.abcam.com\/ps\/products\/83\/ab83367\/Images\/ab83367-290-acid-phosphatase-assay-kit-colorimetric-sample.jpg\" \/><\/p>\n<p><span style=\"color: #800000; font-size: 12pt;\"><strong>\u5f15\u7528\u6587\u737b<\/strong><\/span><\/p>\n<p>Wei Wang. Isofraxidin Inhibits Receptor Activator of Nuclear Factor-?B Ligand\u2013Induced Osteoclastogenesis in Bone Marrow\u2013Derived Macrophages Isolated from Sprague\u2013Dawley Rats by Regulating NF-?B\/NFATc1 and Akt\/NFATc1 Signaling Pathways.\u00a0<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7883151\/\">Cell Transplant. Feb 2021. 33573387.<\/a><\/p>\n<table class=\"table skip-auto-productcode-hyperlinking\">\n<thead>\n<tr>\n<th>Components<\/th>\n<th>Identifier<\/th>\n<th>500 tests<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td title=\"AP Assay buffer\">AP Assay buffer<\/td>\n<td>NM<\/td>\n<td>1 x 100ml<\/td>\n<\/tr>\n<tr>\n<td title=\"AP Enzyme (Lyophilised)\">AP Enzyme (Lyophilised)<\/td>\n<td>Green<\/td>\n<td>1 vial<\/td>\n<\/tr>\n<tr>\n<td title=\"pNpp Substrate\">pNpp Substrate<\/td>\n<td>Red<\/td>\n<td>1 vial<\/td>\n<\/tr>\n<tr>\n<td title=\"Stop Solution\">Stop Solution<\/td>\n<td>WM<\/td>\n<td>1 x 10ml<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<ul class=\"acc__content attributes clearfix\">\n<li class=\"attribute\">\n<h3 class=\"name\">Product name<\/h3>\n<div class=\"value\">Acid Phosphatase Assay Kit (Colorimetric)<br \/>\n<a class=\"see-all-link\" href=\"https:\/\/www.abcam.com\/products?keywords=Acid+phosphatase&amp;selected.productType=Kits&amp;selected.targetName=Acid+phosphatase\">See all Acid phosphatase kits<\/a><\/div>\n<\/li>\n<li class=\"attribute \">\n<h3 class=\"name\">Detection method<\/h3>\n<div class=\"value\">Colorimetric<\/div>\n<\/li>\n<li class=\"attribute\">\n<h3 class=\"name\">Sample type<\/h3>\n<div class=\"value\">Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media<\/div>\n<\/li>\n<li class=\"attribute \">\n<h3 class=\"name\">Assay type<\/h3>\n<div class=\"value\">Enzyme activity<\/div>\n<\/li>\n<li class=\"attribute\">\n<h3 class=\"name\">Sensitivity<\/h3>\n<div class=\"value\">&gt; 0.001 mM<\/div>\n<\/li>\n<li class=\"attribute\">\n<h3 class=\"name\">Range<\/h3>\n<div class=\"value\">0.001 mM &#8211; 1 mM<\/div>\n<\/li>\n<li class=\"attribute\">\n<h3 class=\"name\">Assay time<\/h3>\n<div class=\"value\">1h 10m<\/div>\n<\/li>\n<li class=\"attribute\">\n<h3 class=\"name\">Product overview<\/h3>\n<div class=\"value\">\n<p>Acid Phosphatase Assay Kit ab83367 is a high sensitivity, simple, direct and HTS-ready colorimetric assay designed to measure acid phosphatase activity in serum and other samples.<br \/>\nThe acid phosphatase assay protocol uses\u00a0<em>p<\/em>-nitrophenyl phosphate (<em>p<\/em>NPP) as a phosphatase substrate which turns yellow (absorbance max 405 nm) when dephosphorylated by acid phosphatase.<br \/>\nAcid phosphatase assay protocol summary:<br \/>\n&#8211; add samples and standards to wells<br \/>\n&#8211; add\u00a0<em>p<\/em>NPP to samples<br \/>\n&#8211; add acid phosphatase enzyme to standards<br \/>\n&#8211; incubate for 60 min<br \/>\n&#8211; add stop solution<br \/>\n&#8211; analyze with microplate reader<\/p>\n<\/div>\n<\/li>\n<li class=\"attribute table\">\n<h3 class=\"name\">Notes<\/h3>\n<div class=\"value\">\n<p>This product is manufactured by BioVision, an Abcam company and was previously called K411 Acid Phosphatase Activity Colorimetric Assay Kit. K411-500 is the same size as the 500 test size of ab83367.<\/p>\n<p>Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate the bone disease.<\/p>\n<p><strong>pNPP Assays<\/strong><\/p>\n<p>pNPP assays are a class of phosphatase assays using p-nitrophenyl phosphate (pNPP) as a phosphatase substrate. They can be used to measure alkaline phosphatase, neutral phosphatase, and acid phosphatase activity. These different types of phosphatases are active respectively in alkaline, neutral, and acid assay buffers. pNPP assays include\u00a0<a href=\"https:\/\/www.abcam.com\/alkaline-phosphatase-assay-kit-colorimetric-ab83369.html\">Alkaline Phosphatase Assay Kit ab83369<\/a>\u00a0and Acid Phosphatase Assay Kit ab83367.<\/p>\n<\/div>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><strong>\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u9178\u6027\u78f7\u9178\u9176\u6aa2\u6e2c\u8a66\u5291\u76d2 | \u8ca8\u865fK411-500\u00a0(ABCAM ab83367)\u00a0 Acid Phosphata [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[180],"tags":[2628],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17430"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=17430"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17430\/revisions"}],"predecessor-version":[{"id":37880,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17430\/revisions\/37880"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=17430"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=17430"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=17430"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}