{"id":17413,"date":"2018-08-07T14:57:40","date_gmt":"2018-08-07T06:57:40","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=17413"},"modified":"2022-07-20T16:12:46","modified_gmt":"2022-07-20T08:12:46","slug":"annexin-v-fitc%e7%b4%b0%e8%83%9e%e5%87%8b%e4%ba%a1%e6%aa%a2%e6%b8%ac-aposcreen-annexin-v-apoptosis-kit-fitc-sba%e8%b2%a8%e8%99%9f10010-02","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=17413","title":{"rendered":"Annexin V-FITC\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c | ApoScreen\u00ae Annexin V Apoptosis Kit-FITC SBA\u8ca8\u865f10010-02"},"content":{"rendered":"

Annexin V-<\/strong>FITC<\/strong>\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u00a0 | <\/strong>\u8ca8\u865f<\/strong>10010-0<\/strong><\/a><\/span>2<\/a><\/span>\u00a0 \u00a0\u00a0<\/strong>100<\/strong>\u500b\u53cd\u61c9\u00a0<\/strong><\/p>\n

ApoScreen\u00ae Annexin V Apoptosis Kit-<\/strong>FITC<\/strong><\/p>\n

(Southern Biotech) Annexin V-FITC<\/strong>\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c<\/strong>\u00a0 | <\/strong>\u8ca8\u865f<\/strong>10010-0<\/strong><\/a>2<\/a><\/span><\/strong>\u00a0 \u00a0\u00a0100<\/strong>\u500b\u53cd\u61c9<\/strong> \u00a0<\/span><\/p>\n

(Southern Biotech) ApoScreen\u00ae Propidium Iodide (PI) | 10044-01\u00a0 \u00a0 100 tests \u00a0<\/span><\/p>\n

(Southern Biotech) ApoScreen\u00ae Annexin V Binding Buffer 10X\u00a0 | 10045-01 \u00a0 50 mL \u00a0<\/span><\/p>\n

Apoptosis \u7d30\u80de\u51cb\u4ea1\u4ecb\u7d39-Annexin V\u5206\u6790<\/strong><\/span><\/p>\n

\u7d30\u80de\u51cb\u4ea1\u662f\u6307\u70ba\u7dad\u6301\u6709\u6a5f\u9ad4\u5167\u74b0\u5883\u7a69\u5b9a\uff0c\u7531\u57fa\u56e0\u63a7\u5236\u7684\u7d30\u80de\u81ea\u4e3b\u7684\u6709\u5e8f\u7684\u6b7b\u4ea1\u3002\u6b63\u5e38\u60c5\u6cc1\u4e0b\uff0c\u4efb\u4f55\u7d30\u80de\u5728\u5f62\u6210\u904e\u7a0b\u4e2d\u767c\u751f\u7684\u7570\u5e38\u90fd\u6703\u901a\u904e\u51cb\u4ea1\u6d88\u9664\u3002\u4f8b\u5982\uff0c\u9ad4\u5167\u7684\u764c\u7d30\u80de\u589e\u9577\u70ba\u816b\u7624\u7684\u904e\u7a0b\u6703\u53d7\u7d30\u80de\u51cb\u4ea1\u7684\u5f15\u5c0e\u800c\u88ab\u6291\u5236\u3002\u7136\u800c\uff0c\u5728\u6291\u764c\u57fa\u56e0p53\u51fa\u73fe\u554f\u984c\u6642\uff0c\u51cb\u4ea1\u5c31\u4e0d\u6703\u8a98\u5c0e\u767c\u751f\uff0c\u5f9e\u800c\u5c0e\u81f4\u764c\u7d30\u80de\u7684\u4e0d\u65b7\u589e\u9577\u3002\u7d30\u80de\u51cb\u4ea1\u53ef\u4ee5\u901a\u904e\u7d30\u80de\u5f62\u614b\u7684\u8b8a\u5316\u6216\u751f\u7269\u5316\u5b78\u7684\u8b8a\u5316\u4f86\u6aa2\u6e2c\u3002\u76ee\u524d\u5e38\u7528\u7684\u6307\u6a19\u6709caspase\u6d3b\u6027\u5206\u6790\u3001DNA\u7247\u6bb5\u5206\u6790\u3001\u53ca\u78f7\u8102\u91af\u7d72\u6c28\u9178phosphatidylserine (PS)\u7684\u5916\u7ffb\u7b49\u5be6\u9a57\u3002<\/p>\n

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Human T lymphocyte cell line Jurkat was stained with ApoScreen\u00ae Annexin V-FITC (SB Cat No. 10039-02) and ApoScreen\u00ae 7-Aminoactinomycin D (7-AAD; SB Cat. No. 10042-01) at varying stages of apoptosis. (Green – Annexin V-FITC; 7-AAD – Red)<\/p>\n

Annexin V \u7d30\u80de\u51cb\u4ea1\u5075\u6e2c\u539f\u7406<\/strong><\/p>\n

Annexin V\u7d30\u80de\u67d3\u8272\uff0c\u7528\u65bc\u6aa2\u6e2c\u7d30\u80de\u51cb\u4ea1\u65e9\u671f\u7684\u7d30\u80de\u819c\u8b8a\u5316\u3002\u5728\u7d30\u80de\u51cb\u4ea1\u65e9\u671f\uff0c\u819c\u78f7\u8102\u91af\u7d72\u6c28\u9178phosphatidylserine (PS)\u7531\u8102\u819c\u5167\u5074\u7ffb\u5411\u5916\u5074\u3002Annexin V\u662f\u4e00\u7a2eCa\u96e2\u5b50\u4f9d\u8cf4\u6027\u78f7\u8102\u7d50\u5408\u86cb\u767d\uff0c\u53ef\u4ee5\u8207\u78f7\u8102\u91af\u7d72\u6c28\u9178phosphatidylserine (PS) \u7279\u7570\u7d50\u5408\u3002\u7528\u7da0\u8272\u87a2\u5149FITC\u6a19\u8a18\u6216PE\u6a19\u8a8c\u7684Annexin V\u901a\u904e\u6d41\u5f0f\u7d30\u80de\u5100\u6216\u87a2\u5149\u986f\u5fae\u93e1\u53ef\u4ee5\u6aa2\u6e2c\u5230\u7d30\u80de\u51cb\u4ea1\u7684\u767c\u751f\u3002\u7d50\u5408\u7d30\u80de\u6838\u67d3\u5291PI\u62167-AAD\uff0c\u5229\u7528\u6d41\u5f0f\u7d30\u80de\u5100\u6216\u87a2\u5149\u986f\u5fae\u93e1\u53ef\u5075\u6e2c\u5075\u6e2c\u7d30\u80de\u51cb\u4ea1\u73fe\u8c61\u53ca\u5176\u51cb\u4ea1\u5206\u671f\u3002\u7898\u5316\u4e19\u5576(Propidium Iodide, PI)\u662f\u4e00\u7a2e\u6838\u9178\u67d3\u6599\uff0cPI\u53ea\u80fd\u900f\u904e\u51cb\u4ea1\u665a\u671f\u548c\u6b7b\u7d30\u80de\u7684\u7d30\u80de\u819c\uff0c\u56e0\u6b64Annexin V\u548cPI\u7d50\u5408\u4f7f\u7528\uff0c\u53ef\u4ee5\u5340\u5206\u51cb\u4ea1\u65e9\u665a\u671f\u7684\u7d30\u80de\u53ca\u6b7b\u7d30\u80de\u3002<\/p>\n

\u7d30\u80de\u51cb\u4ea1<\/strong>\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> \u00a0<\/strong>\u7d30\u80de\u51cb\u4ea1\u7684\u65e9\u671f\u968e\u6bb5\uff0c\u5927\u591a\u6578\u7d30\u80de\u5c07\u78f7\u8102\u91af\u7d72\u6c28\u9178\uff08PS\uff09\u5f9e\u8cea\u819c\u7684\u5167\u8868\u9762\u8f49\u79fb\u81f3\u7d30\u80de\u8868\u9762\u3002\u819c\u806f\u86cb\u767dV (Annexin V)\u662f\u4e00\u7a2eCa\u96e2\u5b50\u4f9d\u8cf4\u6027\u78f7\u8102\u7d50\u5408\u86cb\u767d\uff0c\u53ef\u4ee5\u8207\u78f7\u8102\u91af\u7d72\u6c28\u9178phosphatidylserine (PS) \u7279\u7570\u7d50\u5408\u3002\u5957\u7d44\u5167\u5305\u62ec\u6709Annexin V-FITC\u53ca\u7898\u5316\u4e19\u5576\uff08PI\uff09\u3002\u5957\u7d44\u5167\u5305\u62ec\u6709Annexin V-FITC\u53ca\u7898\u5316\u4e19\u5576\uff08PI\uff09\u3002\u61c9\u7528FITC\u7d50\u5408\u6a19\u8a18\u7684Annexin V\u901a\u904e\u6d41\u5f0f\u7d30\u80de\u5100\u6216\u87a2\u5149\u986f\u5fae\u93e1\u53ef\u4ee5\u6aa2\u6e2c\u5230\u7d30\u80de\u51cb\u4ea1\u7684\u767c\u751f\u3002\u7d50\u5408\u7d30\u80de\u6838\u67d3\u5291PI\u540c\u6642\u53ef\u5075\u6e2c\u5075\u6e2c\u7d30\u80de\u51cb\u4ea1\u73fe\u8c61\u53ca\u5176\u51cb\u4ea1\u5206\u671f\u3002\u540c\u6642\u8a55\u4f30\u7d30\u80de\u51cb\u4ea1\u8207\u7d30\u80de\u58de\u6b7b\u6a5f\u5236\u3002\u7576\u7d30\u80de\u7528\u819c\u806f\u86cb\u767dV-FITC\u548c\u7898\u5316\u4e19\u5576\uff08PI\uff09\u96d9\u91cd\u67d3\u8272\u6642\uff0c\u53ef\u4ee5\u89c0\u5bdf\u5230\u4e09\u7a2e\u4e0d\u540c\u7684\u7d30\u80de\u7fa4: \u6d3b\u7d30\u80de\u4e0d\u88abAnnexin V-FITC \u6216 PI\u67d3\u8272 \u3001necrotic cells\u58de\u6b7b\u7d30\u80de\u540c\u6642\u88ab\u5169\u7a2e\u8a66\u5291\u67d3\u8272\u3001\u51cb\u4ea1\u7d30\u80de\u50c5\u53ef\u88ab\u819c\u806f\u86cb\u767dV-FITC\u67d3\u8272\u901a\u904e\u6d41\u5f0f\u7d30\u80de\u5100488nm\u6fc0\u767c\u9032\u884c\u5206\u6790\u3002<\/p>\n

ApoScreen\u00ae\u819c\u806f\u86cb\u767d Annexin V-FITC\u7d30\u80de\u51cb\u4ea1\u8a66\u5291\u76d2 (SBA\u8ca8\u865f10010-05), \u61c9\u7528\u87a2\u5149\u7d20\u7d50\u5408\u7684\u819c\u806f\u86cb\u767dV\uff08\u819c\u806f\u86cb\u767dV-FITC\uff09\u8207\u7898\u5316\u4e19\u5576\uff08PI\uff09\u540c\u6642\u8a55\u4f30\u7d30\u80de\u51cb\u4ea1\u3002\u5728\u7d30\u80de\u51cb\u4ea1\u7684\u65e9\u671f\u968e\u6bb5\uff0c\u7d30\u80de\u958b\u59cb\u5728\u5916\u7d30\u80de\u819c\u4e0a\u986f\u793a\u78f7\u9178\u9170\u7d72\u6c28\u9178\uff08PS\uff09\uff0c\u5176\u4e2d\u901a\u904e\u7528\u819c\u806f\u86cb\u767dV-FITC\u67d3\u8272\u7d30\u80de\u53ef\u4ee5\u5bb9\u6613\u5730\u6aa2\u6e2c\u5230\u5b83\u3002PI\u53ef\u4ee5\u5bb9\u6613\u5730\u7a7f\u904e\u7d30\u80de\u819c\u4e26\u8207\u7d30\u80deDNA\u7d50\u5408\uff0c\u5f9e\u800c\u63d0\u4f9b\u4e86\u901a\u904e\u5305\u62ec\u58de\u6b7b\u5728\u5167\u7684\u6a5f\u5236\u9451\u5b9a\u90a3\u4e9b\u5df2\u7d93\u5931\u53bb\u819c\u5b8c\u6574\u6027\u7684\u7d30\u80de\u7684\u65b9\u6cd5\u3002\u7576\u7d30\u80de\u7528\u819c\u806f\u86cb\u767dV-FITC\u548cPI\u96d9\u91cd\u67d3\u8272\u6642\uff0c\u53ef\u89c0\u5bdf\u5230\u4e09\u7a2e\u4e0d\u540c\u7684\u7d30\u80de\u7fa4 – \uff08i\uff09\u4e0d\u7528\u819c\u806f\u86cb\u767dV-FITC\u6216PI\u67d3\u8272\u7684\u6d3b\u7d30\u80de; \uff08ii\uff09\u7528\u5169\u7a2e\u8a66\u5291\u67d3\u8272\u7684\u58de\u6b7b\u7d30\u80de; \uff08iii\uff09\u50c5\u7528\u819c\u806f\u86cb\u767dV-FITC\u67d3\u8272\u7684\u51cb\u4ea1\u7d30\u80de\u3002\u53ef\u4ee5\u5728\u914d\u5099\u6709\u55ae\u500b\u6fc0\u5149\u7684\u4efb\u4f55\u6d41\u5f0f\u7d30\u80de\u5100\u4e0a\u4f7f\u7528488nm\u7684\u6fc0\u767c\u9032\u884c\u5206\u6790\u3002\u6216\u8005\uff0c\u53ef\u4ee5\u7528\u914d\u5099\u6709\u7528\u65bc\u5340\u5206FITC\u548c\u7f85\u4e39\u660e\/ PI\u7684\u904e\u6ffe\u5668\u7684\u87a2\u5149\u986f\u5fae\u93e1\u6aa2\u67e5\u7d30\u80de\u3002<\/p>\n

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Day old BALB\/c mouse splenocytes were stained with ApoScreen\u00ae Annexin V Apoptosis Kit-FITC (SB Cat. No. 10010-02).<\/p>\n\n\n\n\n\n\n\n
Kit Components<\/strong><\/td>\nAnnexin V-FITC \u2013 100 tests in 1.0 mL
\nPropidium Iodide (PI) \u2013 100 tests in 1.0 mL
\nAnnexin V Binding Buffer 10X \u2013 7 x 1.5 mL<\/td>\n<\/tr>\n
Buffer Formulation<\/strong><\/td>\nAnnexin V-FITC and PI are supplied in Annexin V Binding Buffer<\/td>\n<\/tr>\n
Storage & Handling<\/strong><\/td>\nStore at 2-8\u00b0C
\nPlease refer to product specific\u00a0SDS<\/a><\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nFlow Cytometry \u2013 Quality tested\u00a01-7, 9-34<\/sup>
\nImmunocytochemistry \u2013 Reported in literature\u00a06,8,35<\/sup><\/td>\n<\/tr>\n
Recommended\u00a0Dilutions<\/strong><\/td>\nPlease refer to product specific\u00a0Technical Bulletin<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u7d30\u80de\u514d\u75ab\u5c08\u5bb6<\/strong>\u6975\u529b\u63a8\u85a6<\/strong>SouthernBiotech<\/strong>\u9ad8\u54c1\u8cea\u4e8c\u7d1a\u6297\u9ad4<\/strong><\/p>\n

\u7f8e\u570b\u6297\u9ad4\u751f\u7522\u5546Southern Biotech\u65bc1982\u5e74\uff0c\u7531UAB\u7d30\u80de\u514d\u75ab\u5c08\u5bb6Dr. Max Cooper\u8207\u5be6\u9a57\u5ba4\u6210\u54e1\u5171\u540c\u5275\u7acb\u3002SouthernBiotech\u81f4\u529b\u65bc\u751f\u7522\u9ad8\u54c1\u8cea\u4e8c\u7d1a\u6297\u9ad4\u3002\u7269\u7a2e\u7a2e\u985e\u9f4a\u5168\uff0c\u5305\u62ec\u6709Bovine\u3001Canine\u3001Chicken\u3001Cynomolgus\u3001Donkey\u3001Equine\u3001Feline\u3001Goat\u3001Guinea Pig\u3001Hamster\u3001Human\u3001Monkey\u3001Mouse\u3001Porcine\u3001Rabbit\u3001Rat\u3001Rhesus\u3001Sheep\u3001Turkey\u3002\u5177\u6709\u87a2\u5149\u7269\u8cea\uff08FITC\u3001PE\u3001APC\u3001SPRD\u3001PE\/CY5.5\u3001APC\/CY5.5\u3001Spectral Red\u3001Alexa 488\u3001Alexa 647\u3001Pacific Blue\uff09\u5404\u7a2e\u87a2\u5149\u6a19\u8a8c\u3001\u53e6\u5177\u9175\u7d20\uff08HRP\u3001AP\uff09\u3001\u6216\u672a\u6a19\u8a8c\u53caLow Endotoxin\/Azide Free\uff08LE\/AF\uff09\u7b49\u6a19\u5b9a\u3002<\/p>\n

Isotype control <\/strong>\u5206\u578b\u5c0d\u7167\u7d44<\/strong><\/a> | <\/strong>\u5206\u578b\u5c0d\u7167\u7d44\u5957\u7d44<\/strong><\/a> | <\/strong>\u6297\u9ad4\u5206\u578b<\/strong>ELISA <\/strong>\u5957\u7d44<\/strong><\/a> | <\/strong>anti-rabbit <\/strong>\u4e8c\u7d1a\u6297\u9ad4<\/strong><\/a> | <\/strong>anti-mouse<\/strong>\u4e8c\u7d1a\u6297\u9ad4<\/strong><\/a> | <\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat. No.<\/strong><\/td>\nProduct Description<\/strong><\/td>\nSize<\/strong><\/td>\n<\/tr>\n
10010-02<\/strong><\/a><\/td>\nApoScreen<\/strong>\u00ae<\/sup><\/strong>\u00a0Annexin V Apoptosis Kit-FITC<\/strong><\/td>\n100 tests<\/td>\n<\/tr>\n
10010-09<\/strong><\/a><\/td>\nApoScreen<\/strong>\u00ae<\/sup><\/strong>\u00a0Annexin V Apoptosis Kit-PE<\/strong><\/td>\n100 tests<\/td>\n<\/tr>\n
10039-01<\/strong><\/a><\/td>\nApoScreen<\/strong>\u00ae<\/sup><\/strong>\u00a0Annexin V-UNLB<\/strong><\/td>\n0.1 mg<\/td>\n<\/tr>\n
10039-02<\/strong><\/a><\/td>\nApoScreen<\/strong>\u00ae<\/sup><\/strong>\u00a0Annexin V-FITC<\/strong><\/td>\n100 tests<\/td>\n<\/tr>\n
10039-08<\/strong><\/a><\/td>\nApoScreen<\/strong>\u00ae<\/sup><\/strong>\u00a0Annexin V-BIOT<\/strong><\/td>\n100 tests<\/td>\n<\/tr>\n
10040-01<\/strong><\/a><\/td>\nApoScreen<\/strong>\u00ae<\/sup><\/strong>\u00a0Annexin V-UNLB<\/strong><\/td>\n0.1 mg<\/td>\n<\/tr>\n
10040-02<\/strong><\/a><\/td>\nApoScreen<\/strong>\u00ae<\/sup><\/strong>\u00a0Annexin V-FITC<\/strong><\/td>\n100 tests<\/td>\n<\/tr>\n
10040-08<\/strong><\/a><\/td>\nApoScreen<\/strong>\u00ae<\/sup><\/strong>\u00a0Annexin V-BIOT<\/strong><\/td>\n100 tests<\/td>\n<\/tr>\n
10040-09<\/strong><\/a><\/td>\nApoScreen<\/strong>\u00ae<\/sup><\/strong>\u00a0Annexin V-PE<\/strong><\/td>\n100 tests<\/td>\n<\/tr>\n
10040-11<\/strong><\/a><\/td>\nApoScreen<\/strong>\u00ae<\/sup><\/strong>\u00a0Annexin V-APC<\/strong><\/td>\n100 tests<\/td>\n<\/tr>\n
10040-15<\/strong><\/a><\/td>\nApoScreen<\/strong>\u00ae<\/sup><\/strong>\u00a0Annexin V-CY5<\/strong><\/td>\n100 tests<\/td>\n<\/tr>\n
10042-01<\/strong><\/a><\/td>\nApoScreen<\/strong>\u00ae<\/sup><\/strong>\u00a07-Aminoactinomycin D (7-AAD)<\/strong><\/td>\n100 tests<\/td>\n<\/tr>\n
10044-01<\/strong><\/a><\/td>\nApoScreen<\/strong>\u00ae<\/sup><\/strong>\u00a0Propidium Iodide (PI)<\/strong><\/td>\n100 tests<\/td>\n<\/tr>\n
10045-01<\/strong><\/a><\/td>\nApoScreen<\/strong>\u00ae<\/sup><\/strong>\u00a0Annexin V Binding Buffer 10X<\/strong><\/td>\n50 mL<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

References<\/strong><\/p>\n

    \n
  1. Silkov A, Wolstein O, Shachar I, Dikstein R. Enhanced apoptosis of B and T lymphocytes in TAFII105 dominant-negative transgenic mice is linked to nuclear factor-\u03baB. J Biol Chem. 2002;277:17821-9. (FC)<\/li>\n
  2. Catalan MP, Esteban J, Subir\u00e1 D, Egido J, Ortiz A. Inhibition of caspases improves bacterial clearance in experimental peritonitis. Perit Dial Int. 2003;23:123-6. (FC)<\/li>\n
  3. Lester EA, Babensee JE. Proinflammatory phenotype of endothelial cells after coculture with biomaterial-treated blood cells. J Biomed Mater Res A. 2003;64:397-410. (FC)<\/li>\n
  4. Shiner EK, Terentyev D, Bryan A, Sennoune S, Martinez-Zaguilan R, Li G, et al. Pseudomonas aeruginosa autoinducer modulates host cell responses through calcium signalling. Cell Microbiol. 2006;8:1601-10. (FC)<\/li>\n
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