{"id":17279,"date":"2018-07-16T11:15:57","date_gmt":"2018-07-16T03:15:57","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=17279"},"modified":"2025-08-27T15:49:22","modified_gmt":"2025-08-27T07:49:22","slug":"aposcreen7-aad-%e8%b2%a8%e8%99%9f10042-01","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=17279","title":{"rendered":"7-AAD\u7d30\u80de\u6d3b\u529b\u87a2\u5149\u6307\u793a\u5291 | Ready made solution CAS# 7240-37-1 \u8ca8\u865f10042-01"},"content":{"rendered":"

ApoScreen\u00ae7-AAD\u7d30\u80de\u6d3b\u529b\u87a2\u5149\u6307\u793a\u5291 \u00a0| \u8ca8\u865f10042-01 <\/a>(\u00a0CAS# 7240-37-1 )<\/span><\/strong><\/span><\/p>\n

Synonym:\u00a07-Aminoactinomycin D Ready Made Solution, 7-Aminodactinomycin Ready Made Solution<\/p>\n

 <\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> 7-\u6c28\u57fa – \u653e\u7dda\u83cc\u7d20D (ApoScreen\u00ae7-AAD) \u7528\u65bc\u6d3b\u7d30\u80de\u7684\u6d41\u5f0f\u7d30\u80de\u8853\u5206\u6790\uff0c\u00a07-ADD\u662f\u7d30\u80de\u6d3b\u529b\u87a2\u5149\u6307\u793a\u5291 (Fluorescent DNA stain)\uff1bSu, X., et al.\u00a0\u8a72\u6eb6\u6db2\u53ef\u4ee5\u8207\u85fb\u7d05\u86cb\u767d\uff08PE\uff09\u548c\u87a2\u5149\u7d20\uff08FITC\uff09\u7db4\u5408\u7684\u6297\u9ad4\u4e00\u8d77\u7528\u65bc\u96d9\u8272\u5206\u6790\u3002\u5728\u7d30\u80de\u8868\u9762\u6a19\u8a18\u7269\u67d3\u8272\u5f8c\uff0c\u5c07\u7d30\u80de\u5728PBS\u4e2d\u5728\u51b0\u4e0a\u75287-AAD\u9032\u4e00\u6b65\u67d3\u827230\u5206\u9418\u3002 PBS\u6d17\u6ecc\u5169\u6b21\u5f8c\uff0c\u5c07\u7d30\u80de\u56fa\u5b9a\u5728\u88dc\u5145\u670950\u03bcg\/ ml \u00a0actinomycin D \u653e\u7dda\u83cc\u7d20D\u76841\uff05paraformaldehyde\u591a\u805a\u7532\u919b\u4e2d\u3002\u975e\u51cb\u4ea1\u7d30\u80de\u70ba7-AAD\u9670\u6027\u3002 7-AAD\u8207\u5176\u6bcd\u9ad4\u5206\u5b50Actinomycin D\u4e00\u6a23\uff0c\u662f\u5177\u6709\u751f\u9577\u6291\u5236\u7279\u6027\u7684DNA\u5d4c\u5165\u5291\u30027-AAD\uff0cPE\u548cFITC\u87a2\u5149\u767c\u5c04\u4e4b\u9593\u7684\u5149\u8b5c\u91cd\u758a\u6700\u5c0f\uff0c \u6fc0\u767c\/\u767c\u5c04 – 488\/647 nm\u3002\u7522\u54c1Ready made solution\u5373\u7528\u578b\u578b\u5f0f (10 uL\/106<\/sup> cells,\u00a0final volume of 100 uL )\u30027-AAD\u662fDNA\u67d3\u6599\u3002 \u5b83\u53ef\u4ee5\u7528\u4f86\u4ee3\u66ff\u7898\u5316\u4e19\u5576\uff08PI\uff09\u4f86\u5340\u5206\u6b7b\u7d30\u80de\u3002 \u9019\u7a2e\u67d3\u6599\u975e\u5e38\u9069\u7528\u65bc\u9577\u671f\u5be6\u9a57\u4e2d\u7684\u6b7b\u7d30\u80de\u9451\u5225\uff0c\u4e26\u5177\u6709\u9ad8DNA\u7d50\u5408\u5e38\u6578\u3002<\/p>\n

\"SB2\"<\/a><\/p>\n\n\n\n\n\n\n\n\n
Description<\/strong><\/td>\nApoScreen\u00ae<\/sup>\u00a07-AAD is a ready-to-use cell viability dye for the exclusion of nonviable cells in flow cytometry analysis. This solution can be used in conjunction with phycoerythrin (PE) and fluorescein (FITC) conjugated antibodies in 2-color analysis due to minimal spectral overlap between 7-AAD, PE, and FITC fluorescence emissions. Excitation\/emission \u2013 488\/647 nm<\/td>\n<\/tr>\n
Buffer Formulation<\/strong><\/td>\nSupplied in PBS\/NaN3<\/sub>\u00a0and a stabilizing agent<\/td>\n<\/tr>\n
Volume<\/strong><\/td>\n1.0 mL<\/td>\n<\/tr>\n
Storage & Handling<\/strong><\/td>\nStore at 2-8\u00b0C
\nPlease refer to product specific\u00a0SDS<\/a><\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nFlow Cytometry\u00a01-9<\/sup><\/td>\n<\/tr>\n
Recommended\u00a0Dilutions<\/strong><\/td>\nPlease refer to product specific\u00a0Technical Bulletin<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

References<\/span><\/strong><\/span><\/p>\n

    \n
  1. Sjeklocha LM, Park C, Wong PY, Roney MJ, Belcher JD, Kaufman DS, et al. Erythroid-specific expression of \u03b2-globin from Sleeping Beauty-transduced human hematopoietic progenitor cells. PLoS One. 2011;6(12):e29110. (FC)<\/li>\n
  2. Ateh DD, Leinster VH, Lambert SR, Shah A, Khan A, Walklin HJ, et al. The intracellular uptake of CD95 modified paclitaxel-loaded poly(lactic-co-glycolic acid) microparticles. Biomaterials. 2011;32:8538-47. (FC)<\/li>\n
  3. Domm WG 4th. Optimizing antigen delivery approaches for HIV immunization [dissertation]. Rochester (USA): University of Rochester; 2013. (FC)<\/li>\n
  4. Jenks SA, Palmer E, Marin E, Hartson L, Chida AS, Richardson C, et al. 9G4+ autoantibodies are an important source of apoptotic cell reactivity associated with high levels of disease activity in systemic lupus erythematosus. Arthritis Rheum. 2013;65:3165-75. (FC)<\/li>\n
  5. Sato EI, Machado LS, Machado AC. The effect of ramipril on endothelial function and endothelial progenitor cells in patients with systemic lupus erythematosus [abstract]. Arthritis Rheum. 2013;65 Suppl 10:584, (FC)<\/li>\n
  6. Jones MC, Kobie JJ, DeLouise LA. Characterization of cell seeding and specific capture of B cells in microbubble well arrays. Biomed Microdevices. 2013;15:453-63. (FC)<\/li>\n
  7. De Bruycker M. Invloed van metronomische chemotherapie op myeloid-derived suppressor cellen [thesis]. Ghent (Belgium): Ghent University; 2014 (FC)<\/li>\n
  8. Dewhurst S, Sullivan MA, inventors; University of Rochester, assignee. Antigenic mimics of discontinuous epitopes of pathogen recognized by broadly neutralizing antibodies. United States patent publication US 8,961,977 B2. 2015 Feb 24. (FC)<\/li>\n
  9. Andrade AA, da Gama AN, Teixeira GS, Atella GC, de Castro SL, Coutinho CC, et al. Activity of marine sponge-derived extracts against Trypanosoma cruzi is sssociated with apoptosis-like death. Int J Res Stud Biosci. 2015;3(7):92-101. (FC)<\/li>\n<\/ol>\n

    ApoScreen\u00ae is a registered trademark of SouthernBiotechnology Associates, Inc.<\/p>\n

    SBA\u71b1\u92b7\u5546\u54c1<\/span><\/strong> \u00a0<\/span>7-AAD\u7d30\u80de\u6d3b\u6027\u87a2\u5149\u6307\u793a\u5291<\/a><\/span>\u00a0<\/span>| monocloal\u00a0isotyping\u5957\u7d44<\/a>\u00a0| Annexin V-FITC\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c<\/a><\/span>\u00a0|\u00a0\u00a0DAPI\u87a2\u5149\u5c01\u7247\u5291\u00a0<\/a>\u00a0|\u00a0anti-rabbit\u00a0\u4e8c\u7d1a\u6297\u9ad4<\/a>\u00a0<\/span>|\u00a0anti-mouse\u4e8c\u7d1a\u6297\u9ad4<\/a>\u00a0|\u00a0\u81a0\u539f\u86cb\u767dCollagen Type I\u00a0\u00a0<\/a><\/u><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

    ApoScreen\u00ae7-AAD\u7d30\u80de\u6d3b\u529b\u87a2\u5149\u6307\u793a\u5291 \u00a0| \u8ca8\u865f10042-01 (\u00a0CAS# 7240-37-1 […]<\/p>\n","protected":false},"author":3,"featured_media":6751,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[2655],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17279"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=17279"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17279\/revisions"}],"predecessor-version":[{"id":52459,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17279\/revisions\/52459"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6751"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=17279"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=17279"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=17279"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}