{"id":17001,"date":"2018-06-11T17:04:41","date_gmt":"2018-06-11T09:04:41","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=17001"},"modified":"2023-02-10T13:39:24","modified_gmt":"2023-02-10T05:39:24","slug":"%ce%b1-%e8%91%a1%e8%90%84%e7%b3%96%e8%8b%b7%e9%85%b6%e6%b4%bb%e6%80%a7%e6%aa%a2%e6%b8%ac-%ce%b1-glucosidase-activity-colorimetric-assay-kit%e8%b2%a8%e8%99%9fk690-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=17001","title":{"rendered":"\u03b1-\u8461\u8404\u7cd6\u82f7\u9176\u6d3b\u6027\u6aa2\u6e2c | \u03b1-Glucosidase Activity Colorimetric Assay Kit\u8ca8\u865fK690-100 (abcam ab174093)"},"content":{"rendered":"

\u03b1-\u8461\u8404\u7cd6\u82f7\u9176\u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2 | \u8ca8\u865fK690-100<\/a>\u00a0\u00a0(abcam ab174093)\u00a0<\/span><\/strong><\/span><\/p>\n

\u03b1-Glucosidase Activity Colorimetric Assay Kit<\/em><\/strong><\/span><\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong><\/span>\u03b1-\u8461\u8404\u7cd6\u82f7\u9176 (\u03b1-Glucosidase) \u5c07\u03b1-1,4\u9023\u63a5\u7684\u591a\u91a3(\u03b1-1,4 linked polysaccharides) \u5206\u89e3\u6210\u8461\u8404\u7cd6 (glucose) \uff0c\u5176\u53ef\u4ee5\u7528\u4f5c\u80fd\u91cf\u4f86\u6e90\u3002 \u5728\u751f\u7269\u6280\u8853\u5de5\u696d\u4e2d\uff0c\u03b1-\u8461\u8404\u7cd6\u82f7\u9176 (\u00a0\u03b1-glucosidase)\u7528\u65bc\u9176\u7522\u751f\u7684\u6fb1\u7c89\u6c34\u89e3\u7684\u4e2d\u9593\u5206\u89e3\u7522\u7269\u751f\u7522\u8461\u8404\u7cd6 (glucose)\uff0c\u5982\u6fb1\u7c89\u9176\u7b49\u3002 Pompe\u75c5\u662f\u5df2\u77e5\u768412\u7a2e\u7cd6\u539f\u8caf\u7a4d\u75c5\u4e4b\u4e00 (glycogen storage diseases)\uff0c\u662f\u4e00\u7a2e\u5e38\u67d3\u8272\u9ad4\u96b1\u6027\u907a\u50b3\u4ee3\u8b1d\u6027\u75be\u75c5\uff0c\u6b78\u56e0\u65bc\u03b1-\u8461\u8404\u7cd6\u82f7\u9176\u7f3a\u4e4f\u75c7\u3002 \u5728\u9019\u7a2e\u75be\u75c5\u4e2d\uff0c\u7cd6\u539f\u5728\u6eb6\u9176\u9ad4\u4e2d\u7a4d\u805a\uff0c\u5c0e\u81f4\u9032\u884c\u6027\u808c\u7121\u529b\uff0c\u5fc3\u529b\u8870\u7aed\u548c\u5176\u4ed6\u795e\u7d93\u75c7\u72c0\u3002 \u5728BioVision\u7684\u03b1-\u8461\u8404\u7cd6\u82f7\u9176\u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2\u4e2d\uff0c\u03b1-\u8461\u8404\u7cd6\u82f7\u9176\u6c34\u89e3\u5e95\u7269\u6df7\u5408\u7269\u4ee5\u91cb\u653e\u53ef\u4ee5\u6bd4\u8272\u6e2c\u91cf\u7684\u5c0d\u785d\u57fa\u82ef\u915a\uff08OD = 410nm\uff09\u3002 \u9019\u662f\u4e00\u7a2e\u7c21\u55ae\uff0c\u5feb\u901f\u548c\u9ad8\u901a\u91cf\u7684\u8a66\u5291\u76d2\uff0c\u53ef\u5728\u5404\u7a2e\u6a23\u54c1\u4e2d\u6e2c\u91cf0.1-10 mU\u7684\u03b1-\u8461\u8404\u7cd6\u82f7\u9176\u6d3b\u6027\u3002<\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK690-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (OD 410 nm)<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nMeasurement of \u03b1-Glucosidase activity in biological samples
\nScreening \u03b1-Glucosidase inhibitors<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Rapid and easy-to-use.
\n\u2022 Highly-sensitive & high-throughput capable
\n\u2022 This assay kit can measure 0.1-10 mU of \u03b1-glucosidase activity in a variety of sample.<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 \u03b1-Glucosidase Assay Buffer
\n\u2022 \u03b1-Glucosidase Substrate Mix
\n\u2022 \u03b1-Glucosidase Positive Control
\n\u2022 p-Nitrophenol Standard (100 mM)<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u7522\u54c1\u6ce8\u610f\u4e8b\u9805:<\/strong><\/span><\/p>\n

\u6211\u5011\u5efa\u8b70\u4f7f\u7528\u6211\u5011\u7684\u5206\u6790\u7de9\u885d\u6db2\u9032\u884c\u7d44\u7e54\u52fb\u6f3f\u3002 RIPA\u7de9\u885d\u5340\u901a\u5e38\u5305\u542bSDS\uff0c\u53ef\u80fd\u6703\u5f71\u97ff\u8a66\u5291\u76d2\u4e2d\u9176\u4e4b\u6d3b\u6027\u3002<\/p>\n

\u5efa\u8b70\u5c0d\u6a23\u54c1\u9032\u884c\u591a\u6b21\u7a00\u91cb\uff0c\u4ee5\u78ba\u4fdd\u672a\u77e5\u7269\u7684\u503c\u843d\u5728\u6a19\u6e96\u7684\u9650\u5236\u7bc4\u570d\u5167\u66f2\u7dda\u3002<\/p>\n

Substrate Mix: \u63d0\u4f9b\u70ba\u5373\u7528\u578b\u5305\u88dd\u3002 \u5728-20\u2103\u4e0b\u5132\u5b58\u5f8c\u53ef\u80fd\u5b58\u5728\u986f\u8457\u7684\u6c89\u6fb1\u3002 \u7c21\u77ed\u7684sonication\u8655\u7406\u5373\u53ef\u518d\u6eb6\u89e3\u3002<\/p>\n

\u03b1-\u8461\u8404\u7cd6\u82f7\u9176\u967d\u6027\u5c0d\u7167\uff1a\u7528100\u03bcldH2O\u88fd\u5099\u6210\u00a0stock solution\u3002 \u5e73\u5747\u5206\u70ba10\u03bcl\/\u7ba1\uff0c\u4e26\u5132\u5b58\u5728-20\u00b0C\u5099\u7528\uff0c\u907f\u514d\u51b7\u51cd\/\u89e3\u51cd\uff1b \u5169\u500b\u6708\u5167\u4f7f\u7528\u3002 Working solution\u5de5\u4f5c\u6eb6\u6db2\uff0c\u7a00\u91cb\u70ba10\u500d\u3002 \u5728\u4f7f\u7528\u6642\u4fdd\u6301\u5728\u51b0\u4e0a\u3002 \u4f7f\u7528\u5f8c\u4e1f\u68c4Working solution\u3002<\/p>\n

\u5f15\u7528\u6587\u737b:\u00a0<\/strong><\/span><\/p>\n

\n
Anuwat T et al., (2017) Hypoglycemic and hypolipidemic properties of herbal tea on Wistar rat,\u00a0Chiang Mai Veterinary Journal, 2017, 15(1): 25-35<\/a><\/div>\n<\/div>\n
\n
Tilokwattanothai, A. et al., (2017) Hypoglycemic and hypolipidemic properties of herbal tea on Wistar rat,\u00a0Chiang Mai Veterinary Journal, 2017, 15(1): 25-35<\/a><\/div>\n<\/div>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u03b1-\u8461\u8404\u7cd6\u82f7\u9176\u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2 | \u8ca8\u865fK690-100\u00a0\u00a0(abcam ab174093)\u00a0 \u03b1-Gluc […]<\/p>\n","protected":false},"author":3,"featured_media":17002,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[467],"tags":[2691],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17001"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=17001"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17001\/revisions"}],"predecessor-version":[{"id":38334,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17001\/revisions\/38334"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/17002"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=17001"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=17001"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=17001"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}