{"id":16815,"date":"2018-06-04T11:20:32","date_gmt":"2018-06-04T03:20:32","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=16815"},"modified":"2022-07-20T16:38:41","modified_gmt":"2022-07-20T08:38:41","slug":"apogsh%e7%a9%80%e8%83%b1%e7%94%98%e8%82%bd%e6%af%94%e8%89%b2%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92-glutathione-colorimetric-assay-kit-%e8%b2%a8%e8%99%9fk261-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=16815","title":{"rendered":"ApoGSH\u2122\u7a40\u80f1\u7518\u80bd\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2 |Glutathione Colorimetric Assay Kit \u8ca8\u865fK261-100"},"content":{"rendered":"

ApoGSH\u2122<\/strong>\u7a40\u80f1\u7518\u80bd\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2<\/strong>| <\/strong>\u8ca8\u865f<\/strong>K261-100<\/a><\/span><\/p>\n

Glutathione Colorimetric Assay Kit<\/em><\/strong><\/p>\n

\u7522\u54c1<\/strong>\u63cf\u8ff0\uff1a<\/strong>\u7a40\u80f1\u7518\u80bd\uff08Glutathione, GSH\uff09\u662f\u4e3b\u8981\u7684\u7d30\u80de\u5167\u4f4e\u5206\u5b50\u91cf\u5df0\u57fa\uff0c\u5176\u5728\u7d30\u80de\u9632\u79a6\u54fa\u4e73\u52d5\u7269\u7d30\u80de\u4e2d\u7684\u6c27\u5316\u61c9\u6fc0(\u00a0oxidative stress )\u4e2d\u8d77\u95dc\u9375\u4f5c\u7528\u3002 BioVision\u7684ApoGSH\u2122\u7a40\u80f1\u7518\u80bd\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2 (ApoGSH\u2122 Glutathione Colorimetric Assay Kit, k261) \u63d0\u4f9b\u4e86\u4e00\u7a2e\u65b9\u4fbf\u7684\u6bd4\u8272\u65b9\u6cd5\uff0c\u7528\u5fae\u91cf\u6ef4\u5b9a\u677f\u8b80\u6578\u5100\u5206\u6790\u55ae\u7368\u7684\u7e3d\u7a40\u80f1\u7518\u80bd(total glutathione)\u6216\u9084\u539f\u578b\u7a40\u80f1\u7518\u80bd(reduced form glutathione )\u3002\u8a72\u6e2c\u5b9a\u57fa\u65bcDTNB\u548c\u7a40\u80f1\u7518\u80bd\u9084\u539f\u9176(\u00a0glutathione reductase)\u7684\u7a40\u80f1\u7518\u80bd\u5faa\u74b0\u7cfb\u7d71\uff08\u57161\uff09\u3002 DTNB\u548c\u7a40\u80f1\u7518\u80bd\uff08glutathione , GSH\uff09\u53cd\u61c9\u751f\u6210\u9ec3\u8272\u76842-\u785d\u57fa-5-\u786b\u4ee3\u82ef\u7532\u9178\u3002\u56e0\u6b64\uff0c\u53ef\u4ee5\u901a\u904e\u6e2c\u91cf412nm\u8655\u7684\u5438\u5149\u5ea6\u4f86\u78ba\u5b9aGSH\u6fc3\u5ea6\u3002\u7522\u751f\u7684GSSG\u53ef\u4ee5\u88ab\u7a40\u80f1\u7518\u80bd\u9084\u539f\u9176\u9084\u539f\u6210GSH\uff0c\u4e26\u4e14GSH\u518d\u6b21\u8207DTNB\u53cd\u61c9\u7522\u751f\u66f4\u591a\u76842-\u785d\u57fa-5-\u786b\u4ee3\u82ef\u7532\u9178\u3002\u56e0\u6b64\uff0c\u56de\u6536\u7cfb\u7d71\u986f\u8457\u63d0\u9ad8\u4e86\u7e3d\u7a40\u80f1\u7518\u80bd\u6aa2\u6e2c\u7684\u9748\u654f\u5ea6\u3002\u8a72\u8a66\u5291\u76d2\u5305\u62ec\u7528\u65bc\u5f9e\u6a23\u54c1\u4e2d\u53bb\u9664\u86cb\u767d\u8cea\u4e26\u4fdd\u8b77GSH\u6c27\u5316\u548c\u03b3-\u8c37\u6c28\u9170\u8f49\u80bd\u9176\u53cd\u61c9\u76845-\u78fa\u57fa\u6c34\u694a\u9178\uff08SSA\uff09\u3002\u8a72\u8a66\u5291\u76d2\u53ef\u4ee5\u5728200\u03bcl\u53cd\u61c9\u4e2d\u5f9e1-100ng \/\u5b54\u5b9a\u91cf\u7a40\u80f1\u7518\u80bd\u3002\u70ba\u4e86\u6aa2\u6e2c\u8840\u6db2\u6a23\u54c1\u4e2d\u8f03\u4f4e\u7684\u7a40\u80f1\u7518\u80bd\u6fc3\u5ea6\uff0c\u589e\u52a0\u53cd\u61c9\u6642\u9593\u5c07\u7522\u751f\u66f4\u5f37\u7684\u4fe1\u865f\u3002\u8a72\u8a66\u5291\u76d2\u9084\u53ef\u4ee5\u901a\u904e\u7701\u7565\u53cd\u61c9\u6df7\u5408\u7269\u4e2d\u7684\u7a40\u80f1\u7518\u80bd\u9084\u539f\u9176\u4f86\u7279\u7570\u6027\u6aa2\u6e2c\u9084\u539f\u5f62\u5f0f\u7684\u7a40\u80f1\u7518\u80bd\uff08GSH\uff09\u3002\u6aa2\u6e2c\u9084\u539f\u5f62\u5f0f\u7684\u7a40\u80f1\u7518\u80bd\uff08\u7121\u56de\u6536\u7cfb\u7d71\uff09\u7684\u9748\u654f\u5ea6\u6bd4\u6aa2\u6e2c\u7e3d\u7a40\u80f1\u7518\u80bd\u4f4e100\u500d\u3002<\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK261-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (412 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nThe Colorimetric Glutathione Detection Kit provides a simple in vitro assay for detecting the GSH changes in apoptosis and other pathological processes.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure; takes only ~2-3 hours
\n\u2022 Fast and convenient<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Glutathione Reaction Buffer
\n\u2022 Glutathione Substrate (DTNB)
\n\u2022 NADPH Generating Mix (lyophilized)
\n\u2022 Glutathione Reductase (lyophilized)
\n\u2022 Sulfosalicylic Acid (SSA, 1 gram)
\n\u2022 GSH Standard (lyophilized, MW 307)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5f15\u7528\u6587\u737b<\/strong><\/p>\n

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Jingsi Zhang, et al., (2018) Jia-Jian-Di-Huang-Yin-Zi decoction exerts neuroprotective effects on dopaminergic neurons and their microenvironment,\u00a0Scientific Reports, Jun. 2018, 29959371<\/a><\/div>\n<\/div>\n
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Park, S. et al., (2017) Proteasome inhibitor-induced cleavage of HSP90 is mediated by ROS generation and caspase 10-activation in human leukemic cells,\u00a0Redox Biology, Oct.2017, 13: 470-476<\/a><\/div>\n<\/div>\n
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Wang, Y. et al., (2017) Quercetin and cyanidin-3-glucoside protect against photooxidation and photodegradation of A2E in retinal pigment epithelial cells,\u00a0Experimental Eye Research, Jul.2017, 160:45-55<\/a><\/div>\n<\/div>\n
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Yuan, CW et al., (2017) Evaluation of efficacy and toxicity of exfoliated silicate nanoclays as a feed additive for fumonisin detoxification,\u00a0J. Agric. Food Chem, 2017, Just accepted<\/a><\/div>\n<\/div>\n
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Roh, Jong-Lyel et al. (2017) Nrf2 inhibition reverses the resistance of cisplatin-resistant head and neck cancer cells to artesunate-induced ferroptosis,\u00a0Redox Biol. 2017 Apr;11:254-262.<\/a><\/div>\n<\/div>\n

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\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

ApoGSH\u2122\u7a40\u80f1\u7518\u80bd\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2| \u8ca8\u865fK261-100 Glutathione Colorimetric […]<\/p>\n","protected":false},"author":3,"featured_media":16816,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[122],"tags":[2697],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/16815"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=16815"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/16815\/revisions"}],"predecessor-version":[{"id":19980,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/16815\/revisions\/19980"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/16816"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=16815"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=16815"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=16815"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}