{"id":16790,"date":"2018-06-01T09:19:37","date_gmt":"2018-06-01T01:19:37","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=16790"},"modified":"2022-07-20T16:39:12","modified_gmt":"2022-07-20T08:39:12","slug":"reca-protein-e-coli-%e8%b2%a8%e8%99%9f-e024","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=16790","title":{"rendered":"RecA Protein, E. coli | \u8ca8\u865f E024"},"content":{"rendered":"<p>DNA &amp; RNA Modifying Enzymes<\/p>\n<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=16790\"><strong>RecA Protein, E. coli | <\/strong><strong>\u8ca8\u865f<\/strong> E024<\/a><\/span><\/p>\n<p>ABM\u63d0\u4f9b\u4e86\u4e00\u6574\u5957\u6d89\u53caDNA\u548cRNA\u4fee\u98fe\u548c\u4fee\u5fa9\u7684\u86cb\u767d\u8cea\u3002 \u5728\u7e7c\u7e8c\u767c\u5c55\u7684\u540c\u6642\uff0c\u76ee\u524dABM\u5c08\u7528\u514b\u9686\u6280\u8853\u9176\u7684\u76ee\u9304\u5305\u62ecligases\u9023\u63a5\u9176\uff0cphosphatases\u78f7\u9178\u9176\uff0cnucleases\u6838\u9178\u9176\u548cmesophilic\u55dc\u6eabDNA\u805a\u5408\u9176\u7b49\u7b49\u3002<\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u5546\u54c1<\/strong><strong>:<\/strong> <b>RecA Protein<\/b>\u4f86\u81ea\u5927\u8178\u687f\u83cc\u7684RecA\u662f\u53c3\u8207ATP\u4f9d\u8cf4\u6027\u904e\u7a0b\u4e2d\u7684\u540c\u6e90\u91cd\u7d44\u7684DNA\u7d50\u5408\u86cb\u767d\u3002 RecA\u8207\u55ae\u93c8DNA\u7d50\u5408\u5f62\u6210\u6838\u86cb\u767d\u8907\u5408\u7269\uff0c\u4e26\u4fc3\u9032\u55ae\u93c8DNA\u7247\u6bb5\u8207\u540c\u6e90\u96d9\u93c8DNA\u7684\u93c8\u4ea4\u63db\u3002 RecA\u9084\u5728\u5f8c\u5fa9\u5236DNA\u4fee\u5fa9\u6a5f\u5236\u548cDNA\u4fee\u5fa9\u4ee5\u53caUV\u8a98\u5c0e\u7684\u8a98\u8b8a\u4e2d\u767c\u63ee\u4f5c\u7528\u3002 RecA\u86cb\u767d\u901a\u5e38\u7528\u65bc\u7814\u7a76\u6d89\u53ca\u540c\u6e90\u91cd\u7d44\u7684\u5206\u5b50\u6a5f\u5236\u3002\u53ef\u61c9\u7528\u65bcdisplacement loop mutagenesis\u7f6e\u63db\u74b0\u8a98\u8b8a\u3001targeted DNA cleavage\u9776\u5411DNA\u5207\u5272\u3001\u7528\u96fb\u5b50\u986f\u5fae\u93e1\u89c0\u5bdfDNA\u3001\u7528RecA\u5305\u88ab\u63a2\u91dd\u9032\u884clibrary screening<\/p>\n<table width=\"100%\">\n<tbody>\n<tr>\n<td><strong>Form<\/strong><\/td>\n<td>Protein supplied with 10X Reaction Buffer.<\/td>\n<\/tr>\n<tr>\n<td><strong>Concentration<\/strong><\/td>\n<td>2.0 mg\/ml<\/td>\n<\/tr>\n<tr>\n<td><strong>Storage<\/strong><\/td>\n<td>Store all components at -20 \u00b0C.<\/td>\n<\/tr>\n<tr>\n<td><strong>Storage Buffer<\/strong><\/td>\n<td>10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50% (v\/v) Glycerol.<\/td>\n<\/tr>\n<tr>\n<td><strong>Notes<\/strong><\/td>\n<td>This product is distributed for laboratory research only. Caution: Not for diagnostic use.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>DNA &amp; RNA Modifying Enzymes RecA Protein, E. coli | [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":16792,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[2701,2700],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/16790"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=16790"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/16790\/revisions"}],"predecessor-version":[{"id":16794,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/16790\/revisions\/16794"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/16792"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=16790"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=16790"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=16790"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}