{"id":16678,"date":"2018-05-26T18:44:19","date_gmt":"2018-05-26T10:44:19","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=16678"},"modified":"2023-08-21T12:27:04","modified_gmt":"2023-08-21T04:27:04","slug":"%e8%86%a0%e5%8e%9f%e9%85%b6%ef%bc%88%e8%86%a0%e5%8e%9f%e9%99%8d%e8%a7%a3%e9%85%b6%e8%ad%9c%e6%b3%95%ef%bc%89%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92%ef%bd%9ccollagenase-collagen-degradationzym","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=16678","title":{"rendered":"\u81a0\u539f\u9176\uff08\u81a0\u539f\u964d\u89e3\/\u9176\u8b5c\u6cd5\uff09\u6e2c\u5b9a\u8a66\u5291\u76d2\uff5cCollagenase (Collagen Degradation\/Zymography) Assay Kit \u8ca8\u865fK490-100 (abcam ab234624)"},"content":{"rendered":"

\u81a0\u539f\u9176\uff08\u81a0\u539f\u964d\u89e3\/\u9176\u8b5c\u6cd5\uff09\u6e2c\u5b9a\u8a66\u5291\u76d2\uff08<\/strong>\u87a2\u5149\u6cd5\uff09\uff5c<\/strong>(ab234624)\u8ca8\u865fK490-100\u00a0<\/a><\/span><\/p>\n

Collagenase (Collagen Degradation\/Zymography) Assay Kit (Fluorometric) <\/strong><\/p>\n

\u7522\u54c1\u4ecb\u7d39:<\/strong> Collagenases\u81a0\u539f\u9176\u662f\u57fa\u8cea\u91d1\u5c6c\u86cb\u767d\u9176matrix metalloproteinase (MMP)\u5bb6\u65cf\u7684\u6210\u54e1\uff0c\u662f\u6d88\u5316\u4e09\u87ba\u65cb\u5340\u57df(triple helix region)\u4e2d\u7684\u5929\u7136\u81a0\u539f,\u4e26\u53c3\u8207\u7d50\u7de0\u7d44\u7e54\u7684\u751f\u7406\u548c\u75c5\u7406turnover\u7684\u5167\u80bd\u9176\u3002\u81a0\u539f\u86cb\u767d\u662f\u52d5\u7269\u7d30\u80de\u5916\u7d50\u7de0\u7d44\u7e54\u7684\u4e3b\u8981\u7e96\u7dad\u7d44\u5206(fibrous component)\u3002\u7d30\u83cc\u81a0\u539f\u9176(Bacterial collagenases)\u4e0d\u540c\u65bc\u810a\u690e\u52d5\u7269\u81a0\u539f\u9176\uff0c\u56e0\u70ba\u5b83\u5011\u8868\u73fe\u51fa\u66f4\u5bec\u7279\u7570\u6027\u3002\u81a0\u539f\u9176(Collagenases)\u53ef\u80fd\u5728\u8f49\u79fb\u904e\u7a0b\u7684\u591a\u500b\u968e\u6bb5\u4fc3\u9032\u816b\u7624(metastatic)\u7d30\u80de\u4fb5\u5165\u7d30\u80de(cell invasion)\u5916\u57fa\u8cea(extracellular matrix)\u3002\u81a0\u539f\u9176\u4e5f\u88ab\u6279\u51c6\u7528\u65bc\u91ab\u7642\u7528\u9014\uff0c\u7528\u65bc\u6cbb\u7642Dupuytren’s\u6523\u7e2e\u75c7\uff0cPeyronie’s disease\u4f69\u7f85\u5c3c\u6c0f\u75c5\u548c\u50b7\u53e3\u7652\u5408\u3002\u81a0\u539f\u9176\u6d3b\u6027\u6e2c\u5b9a\u5176\u61c9\u7528\u539f\u7406\u901a\u5e38\u4ee5\u5c0f\u80bd\u7684\u6d3b\u6027\u6e2c\u5b9a\u6aa2\u6e2c\u81a0\u539f\u9176\u6d3b\u6027\uff0c\u5176\u53ef\u80fd\u7f3a\u4e4f\u5e95\u7269\u7279\u7570\u6027\u3002\u81a0\u539f\u9176\u6d3b\u6027\u7684\u5176\u4ed6\u65b9\u6cd5\u5305\u62ec\u81a0\u539f\u86cb\u767d\u9176\u8b5c\u6cd5\uff0c\u5176\u4e2d\u6a23\u54c1\u5728\u542b\u81a0\u539f\u7684SDS-PAGE\u4e0a\u96fb\u6cf3\uff0c\u4e26\u9032\u4e00\u6b65\u5728\u5408\u9069\u7684\u7de9\u885d\u6db2\u4e2d\u5fa9\u602712-16\u5c0f\u6642\u3002zymogram\u9176\u8b5c\u96a8\u5f8c\u88ab\u67d3\u8272\uff0c\u4e26\u4e14\u6d88\u5316\u5340\u57df\u5c0d\u65bc\u5e95\u7269\u5df2\u7d93\u88ab\u9176\u964d\u89e3\u7684\u6df1\u8272\u67d3\u8272\u80cc\u666f\u986f\u73fe\u70ba\u6e05\u6670\u689d\u5e36\u3002\u9019\u7a2e\u65b9\u6cd5\u8cbb\u6642\u8cbb\u529b\uff0c\u4e26\u4e14\u53ef\u80fd\u5c0e\u81f4\u9176\u6d3b\u6027\u7684\u55aa\u5931\uff0c\u53ef\u80fd\u4e0d\u5b8c\u5168\u53ef\u9006reversible\u3002BioVision\u7684\u81a0\u539f\u9176\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2\u63a1\u7528\u6df7\u5408\u65b9\u6cd5\u6aa2\u6e2c\u81a0\u539f\u9176\u6d3b\u6027\uff0c\u63a1\u7528\u9ad8\u5ea6\u6dec\u6ec5\u7684\u81a0\u539f\u86cb\u767d\u5e95\u7269\uff0c\u901a\u904e\u9069\u7576\u7684\u81a0\u539f\u9176\u5207\u5272\u91cb\u653e\u87a2\u5149\u5718fluorophore\uff0c\u53ef\u4ee5\u4f7f\u7528\u5e38\u898fmicroplate reader\u8f15\u9b06\u5b9a\u91cf\u3002\u8a72\u65b9\u6cd5\u5177\u6709\u5e95\u7269\u7279\u7570\u6027\uff0c\u7c21\u55ae\uff0c\u5feb\u901f\uff0c\u9ad8\u901a\u91cf\u9069\u61c9\u6027\uff0c\u4e26\u4e14\u9069\u7528\u65bc\u751f\u7269\u6a23\u54c1\u4e2d\u81a0\u539f\u9176\u6d3b\u6027\u7684\u9748\u654f\u6aa2\u6e2c\uff08\u4f4e\u81f30.6 mCDU\u7684\u7d30\u83cc\u81a0\u539f\u9176)<\/p>\n

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Cat # +Size<\/td>\nK490-100 (ab234624)<\/td>\n<\/tr>\n
Size<\/td>\n100 assays<\/td>\n<\/tr>\n
Kit Summary<\/td>\n\u2022 Detection method- Fluorometric (490\/520 nm) \u2022 Species reactivity- mammalian \u2022 Applications- – Measurement of collagenase activity – For screening\/studying\/characterizing collagenase inhibitors<\/td>\n<\/tr>\n
Detection Method<\/td>\nFluorescence (490\/520 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/td>\n\u2022 Measurement of collagenase activity \u2022 For screening\/studying\/characterizing collagenase inhibitors<\/td>\n<\/tr>\n
Features & Benefits<\/td>\n\u2022 Rapid & sensitive \u2022 Simple assay to measure collagenase activity as well as to screen\/study\/characterize potential inhibitors of collagenase \u2022 Includes Fluorometric Collagenase Substrate and Positive Control<\/td>\n<\/tr>\n
Kit Components<\/td>\n\u2022 Collagenase Assay Buffer \u2022 Cell Lysis Buffer \u2022 Enzyme Positive Control \u2022 Collagenase (Substrate) \u2022 FITC Standard (5 mM)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

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\u5176\u4ed6\u5546\u54c1: <\/strong>\u81bd\u56fa\u9187\u6392\u51fa\u87a2\u5149\u5206\u6790\u5957\u7d44<\/a> | CETP\u81bd\u56fa\u9187\u916f\u8f49\u904b\u86cb\u767dELISA<\/a> | Irisin\u7af6\u722d\u578bELISA<\/a> | Free Fatty Acid\u5448\u8272\u87a2\u5149\u5206\u6790<\/a> | Triglyceride\u4e09\u9178\u7518\u6cb9\u916f\u5b9a\u91cf\u5206\u6790<\/a> \u3010\u67d3\u8272\u5546\u54c1\u3011<\/strong> Lipid\u8102\u8cea\u67d3\u8272\u5957\u7d44<\/a> | \u81bd\u56fa\u9187\u67d3\u8272\u5957\u7d44<\/a> \u3010\u8403\u53d6\u5546\u54c1\u3011<\/strong>\u8102\u80aa\u7d44\u7e54\u86cb\u767d\u8cea\u8403\u53d6<\/a><\/p>\n

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\u00a0<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

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