{"id":16673,"date":"2018-05-26T18:25:21","date_gmt":"2018-05-26T10:25:21","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=16673"},"modified":"2024-04-12T17:19:38","modified_gmt":"2024-04-12T09:19:38","slug":"%e7%b8%bd%e8%86%a0%e5%8e%9f%e5%90%ab%e9%87%8f%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92%ef%bd%9ctotal-collagen-assay-kit-perchlorate-free-%e8%b2%a8%e8%99%9fk406-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=16673","title":{"rendered":"\u7e3d\u81a0\u539f\u542b\u91cf\u6e2c\u5b9a\u8a66\u5291\u76d2\uff5cTotal Collagen Assay Kit (Perchlorate-Free) \u8ca8\u865fK406-100 abcam ab222942"},"content":{"rendered":"

\u7e3d\u81a0\u539f\u542b\u91cf\u6e2c\u5b9a\u8a66\u5291\u76d2\uff08\u7121\u9ad8\u6c2f\u9178\u9e7d\uff09\uff5c<\/span><\/strong>\u8ca8\u865fK406-100<\/span><\/a>\u00a0 (abcam ab222942)<\/span><\/span><\/p>\n

Total Collagen Assay Kit (Perchlorate-Free)\u00a00.5\u03bcg\u81a0\u539f\u86cb\u767d<\/strong><\/span><\/p>\n

\u81a0\u539f\u86cb\u767d(Collagen )\u662f\u54fa\u4e73\u52d5\u7269\u4e2d\u542b\u91cf\u6700\u8c50\u5bcc\u7684\u86cb\u767d\u8cea\uff0c\u4f54\u9ad4\u5167\u86cb\u767d\u8cea\u542b\u91cf\u7684\u8fd130\uff05\u3002\u81a0\u539f\u86cb\u767d\u662f\u5728\u76ae\u819a(skin)\uff0c\u808c\u8171(tendons)\uff0c\u9aa8(\u00a0bone)\uff0c\u8edf\u9aa8(\u00a0cartilage)\uff0c\u97cc\u5e36(\u00a0ligaments )\u548c\u808c\u8089(\u00a0muscles)\u7b49\u7d44\u7e54\u4e2d\u767c\u73fe\u7684\u7d30\u80de\u5916\u652f\u67b6\u86cb\u767d(extracellular scaffolding protein )\u3002\u6709\u8d85\u904e20\u7a2e\u4e0d\u540c\u7684\u7279\u5fb5\u985e\u578b\u7684\u81a0\u539f\u86cb\u767d\uff0c\u4f46\u662f\u7e96\u7dad\u985e\u578bI(\u00a0fibrilous types I)\uff0cII(\u00a0fibrilous types II)\u548cIII(\u00a0fibrilous types III)\u4f54\u54fa\u4e73\u52d5\u7269\u7e3d\u81a0\u539f\u768490\uff05\u4ee5\u4e0a\u3002\u81a0\u539f\u86cb\u767d\u5177\u6709\u4e09\u87ba\u65cb\u7d50\u69cb(triple helical structure)\uff0c\u7531\u4e09\u500b\u5177\u6709\u91cd\u8907Gly-X-Hyp\u4e09\u80bd\u5e8f\u5217\u7684’\u524d\u539f\u81a0\u539f'(preprocollagen) \u93c8\u7d44\u6210\uff0c\u5176\u4e2dX\u662f\u4efb\u4f55\u6c28\u57fa\u9178\uff0cHyp\u662f\u7fa5\u812f\u6c28\u9178(hydroxyproline)\uff0c\u7ffb\u8b6f\u5f8c\u4fee\u98fe\u7684\u6c28\u57fa\u9178\u7531\u812f\u6c28\u9178 (proline) \u901a\u904e\u812f\u6c28\u9170-4-\u7fa5\u5316\u9176 (prolyl-4- hydroxylase)\u3002\u5728\u52d5\u7269\u4e2d\uff0c\u7fa5\u812f\u6c28\u9178 (hydroxyproline )\u5e7e\u4e4e\u5168\u90e8\u5b58\u5728\u65bc\u81a0\u539f\u86cb\u767d\uff08\u5176\u8cea\u91cf\u542b\u6709\u5927\u7d0412-14\uff05\u7684\u7fa5\u812f\u6c28\u9178\uff09\u4e26\u4e14\u6709\u52a9\u65bc\u5176\u7d50\u69cb\u525b\u6027\u548c\u9ad8\u62c9\u4f38\u5f37\u5ea6\u3002\u7fa5\u812f\u6c28\u9178(Hydroxyproline)\u56e0\u6b64\u662f\u7d44\u7e54\u4e2d\u5b58\u5728\u7684\u81a0\u539f\u91cf\u7684\u76f4\u63a5\u6e2c\u91cf\u6307\u6a19\u3002\u64da\u4fe1\u8a31\u591a\u75be\u75c5\u5f71\u97ff\u81a0\u539f\u86cb\u767d\u7684\u5468\u8f49(collagen turnover)\uff0c\u5305\u62ec\u816b\u7624\u4fb5\u8972(tumor invasion)\u548c\u8f49\u79fb(metastasis)\uff0c\u985e\u98a8\u6fd5\u6027\u95dc\u7bc0\u708e(rheumatoid arthritis)\uff0c\u5fc3\u80ba\u7e96\u7dad\u5316(cardiopulmonary fibrosis )\u548c\u808c\u71df\u990a\u4e0d\u826f(muscular dystrophy)\u3002<\/p>\n

\"\"<\/p>\n

BioVision\u7684Total Collagen Assay Kit II, K406\uff08Perchlorate-Free, \u4e0d\u542b\u9ad8\u6c2f\u9178\u9e7d\uff09\u662f\u4e00\u7a2e\u7528\u65bc\u6aa2\u6e2c\u5404\u7a2e\u751f\u7269\u6a23\u54c1\u4e2d\u5c11\u91cf\u81a0\u539f\u86cb\u767d\u7684\u5feb\u6377\u65b9\u4fbf\u7a0b\u5e8f\u3002\u8a72\u6e2c\u5b9a\u57fa\u65bc\u6a23\u54c1\u7684\u6c34\u89e3(\u00a0hydrolysis )\u4ee5\u7522\u751f\u6e38\u96e2\u7684Hyp\uff0c\u5176\u88ab\u6c27\u5316\u4e26\u53cd\u61c9\u4ee5\u7522\u751f\u5728560nm\u8655\u5177\u6709\u5438\u6536\u5cf0\u7684\u4eae\u8272\u9aee\u8272\u5718\u3002\u8a72\u6aa2\u6e2c\u65b9\u6cd5\u63a1\u7528\u5c08\u6709\u7684\u9178\u6027\u986f\u5f71\u6db2 (acidic developer solution) \uff0c\u7121\u9700\u4f7f\u7528\u6709\u5bb3\u7684\u9ad8\u6c2f\u9178(perchloric acid)\u5373\u53ef\u7cbe\u78ba\u6e2c\u91cf\u6c34\u89e3\u6db2\u4e2d\u7684\u81a0\u539f\u86cb\u767d\uff0c\u7121\u9700\u4efb\u4f55\u7279\u6b8a\u8655\u7406\u548c\u5ee2\u7269\u8655\u7406\u65b9\u6848\u3002\u8a72\u6e2c\u5b9a\u6cd5\u53ef\u4ee5\u6aa2\u6e2c96\u5b54\u683c\u5f0f\u4e2d\u6bcf\u5b54\u6700\u5c110.5\u03bcg\u81a0\u539f\u86cb\u767d<\/span>\uff0c\u4e26\u7522\u751f\u8207\u50b3\u7d71\u57fa\u65bc\u9ad8\u6c2f\u9178\u7684\u65b9\u6cd5\u5be6\u969b\u4e0a\u76f8\u540c\u7684\u6bd4\u8272\u7d50\u679c\u3002\u6e2c\u8a66\u7684\u6a23\u54c1\u4f86\u6e90\uff0c\u53ef\u4ee5\u70ba\u7d44\u7e54\u5747\u8cea\u6db2 (Tissue homogenates, i.e. muscle, liver, lung, etc.)\u00a0\u4eba\u985e\u6216\u52d5\u7269\u751f\u7269\u6db2\u9ad4 (biological fluids , serum, urine) \u6b64\u7522\u54c1\u9700\u7531\u5100\u5668\u9032\u884c\u5b9a\u91cf\u00a0ELISA\u5100\u5668\u8a2d\u5099\u8acb\u6309\u6b64<\/a><\/span><\/strong><\/p>\n

\u2022 Simple, fast and convenient assay
\n\u2022 Can detect as low as 0.5 \u00b5g of collagen in a variety of samples
\n\u2022 Simple procedure; takes less than ~60 minutes
\n\u2022 Faster than Perchlorate-Based Assay
\n\u2022 PCA-free<\/p>\n

\"ELISA<\/a><\/p>\n

\"K406\"<\/a><\/p>\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/td>\nK406-100<\/td>\n<\/tr>\n
Size<\/td>\n100 Reactions<\/td>\n<\/tr>\n
Kit Summary<\/td>\n\u2022 Detection method- Absorbance (OD = 560 nm). \u2022 Sample Types- Mammalian tissues, protein\/peptide hydrolysates, serum, Urine \u2022 Applications- Measurement of collagen in various sample types.<\/td>\n<\/tr>\n
Detection Method<\/td>\nDetection method- Absorbance (OD = 560 nm).<\/td>\n<\/tr>\n
Species Reactivity<\/td>\nMammalian tissues, protein\/peptide hydrolysates, serum, urine<\/td>\n<\/tr>\n
Applications<\/td>\nMeasurement of collagen in various sample types.<\/td>\n<\/tr>\n
Features & Benefits<\/td>\n\u2022 Simple, fast and convenient assay \u2022 Can detect as low as 0.5 \u00b5g of collagen in a variety of samples \u2022 Simple procedure; takes less than ~60 minutes \u2022 Faster than Perchlorate-Based Assay \u2022 PCA-free<\/td>\n<\/tr>\n
Kit Components<\/td>\n\u2022 Oxidation Buffer \u2022 Chloramine T Concentrate \u2022 Developer Solution \u2022 DMAB Concentrate (in DMSO) \u2022 Collagen I Standard (3 mg\/ml) \u2022 Microplate Sealing Film<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

For hydrolysis: Polypropylene Vials (BV Cat. No. M1352) and Screw Caps (BV Cat. No. M1353)<\/p>\n

\"\"<\/p>\n

\u7522\u54c1\u4f7f\u7528\u6ce8\u610f\u4e8b\u9805:<\/strong> \u91dd\u5c0d\u6a23\u54c1\u6c34\u89e3(\u00a0hydrolysis )\u6b65\u9a5f\uff0c\u5efa\u8b70\u4f7f\u7528\u805a\u4e19\u70ef\u6a23\u54c1\u74f6\u53ef\u7372\u5f97\u6700\u4f73\u7d50\u679c\u00a0Polypropylene Vials\u805a\u4e19\u70ef\u5c0f\u74f6 (BV Cat. No. M1352) and Screw Caps (BV Cat. No. M1353)\u3002\u975e\u5e38\u5805\u97cc\u7684\u6a23\u54c1\uff08\u542b\u6709\u9aa8\u9abc\u6216\u5916\u9aa8\u9abc\u7d44\u7e54\uff09\u5728120\u5ea6C\u6c34\u89e3\u6b65\u9a5f\uff0c\u53ef\u80fd\u9700\u8981\u52a0\u71b1\u8d85\u904e1\u5c0f\u6642\u624d\u80fd\u5b8c\u5168\u6c34\u89e3\u3002\u67d0\u4e9b\u6a23\u54c1\uff08\u4f8b\u5982\u8102\u80aa\u7d44\u7e54\uff09\u7684\u6c34\u89e3\u7522\u7269\u53ef\u80fd\u542b\u6709\u96e3\u4ee5\u901a\u904e\u96e2\u5fc3\u6c89\u6fb1\u7684\u8102\u8cea\u788e\u7247\u3002\u79fb\u53d6\u6c34\u89e3\u6a23\u54c1\u4ee5\u907f\u514d\u5c07\u9019\u4e9b\u4e0d\u6eb6\u6027\u5c0f\u7403\u8f49\u79fb\u5230\u6a23\u54c1\u5b54\u4e2d\u3002\u5c0d\u65bc\u672a\u77e5\u6a23\u54c1\uff0c\u6211\u5011\u5efa\u8b70\u9032\u884c\u8a66\u9a57\u6027\u5be6\u9a57\uff0c\u4ee5\u78ba\u4fdd\u8b80\u6578\u5728\u6a19\u6e96\u66f2\u7dda\u7bc4\u570d\u5167\u76f8\u61c9\u5730\u8abf\u7bc0\u6a23\u54c1\u6c34\u89e3\u7522\u7269\u7684\u9ad4\u7a4d\uff08\u53ef\u4ee5\u4f7f\u75282-20\u03bcl\u6c34\u89e3\u7522\u7269\uff09\u6216\u5fc5\u8981\u6642\u7a00\u91cb\u6c34\u89e3\u7522\u7269\u3002\u7522\u54c1\u7121\u63d0\u4f9b<\/span> Clear 96-well plates with flat bottom \u3001 10N sodium hydroxide (NaOH) \u300110N hydrochloric acid (HCl) \u3001Polypropylene Vials (BV Cat. No. M1352) \u3001 Screw Caps (BV Cat. No. M1353)<\/p>\n

Polypropylene Vials<\/strong>\u805a\u4e19\u70ef\u5c0f\u74f6\u00a0<\/strong>|\u00a0<\/strong>\u8ca8\u865fM1352<\/strong><\/a><\/span><\/p>\n

\"m1353-2\"<\/p>\n

Non-pyrogenic, non-cytotoxic polypropylene vials. Caps sold separately (BV# M1353)<\/em><\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong>BioVision\u7684\u87ba\u65cb\u84cb\u7531\u9ad8\u7d14\u5ea6\uff0c\u4f4e\u91d1\u5c6c\u542b\u91cf\u7684\u805a\u4e19\u70ef\u5171\u805a\u7269\u6a39\u8102\u6a21\u5851\u800c\u6210\u3002 \u87ba\u65cb\u84cb\u57287.5 psig\u4e0b\u4e00\u8d77\u9032\u884c\u58d3\u529b\u6e2c\u8a66\uff0c\u4ee5\u78ba\u4fdd\u7a7a\u904b\u5b89\u5168\u3002 \u6a23\u54c1\u74f6\u55ae\u7368\u51fa\u552e\uff08BV\uff03M1352\uff09\u3002<\/p>\n\n\n\n\n\n\n
Highlights<\/strong><\/td>\n\u2022 USP Class VI compliant; nonpyrogenic, noncytotoxic
\n\u2022 Caps sold separately (BV# M1353)
\n\u2022 Nonsterile
\n\u2022 Leakproof when used with BioVision\u2019s Caps (BV# M1353)
\n\u2022 Works with blue light\/LED
\n\u2022 Color: Natural, translucent
\n\u2022 Skirted with conical interior
\n\u2022 Diameter (Metric) Outside 13 mm; Height (Metric) 49 mm; Diameter (Metric) Inside: 8 mm
\n\u2022 This item is recommended for Cat# K206, K226, K406 and K555<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\nRT<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n
USAGE<\/strong><\/td>\nFor Research Use Only! Not For Use in Humans.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Screw Caps\u00a0<\/strong>|\u00a0<\/strong>\u8ca8\u865fM1353<\/strong><\/a><\/span><\/p>\n

Low Profile, non-pyrogenic, noncytotoxic polypropylene screw caps. Vials sold separately (BV# M1352).<\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong>BioVision\u7684\u805a\u4e19\u70ef\u5c0f\u74f6\u7531\u9ad8\u7d14\u5ea6\uff0c\u7121\u7d30\u80de\u6bd2\u6027\uff0c\u7121\u71b1\u539f\uff0c\u4f4e\u91d1\u5c6c\u542b\u91cf\u7684\u805a\u4e19\u70ef\u5171\u805a\u7269\u6a21\u5851\u800c\u6210\u3002 \u5c0f\u74f6\u57287.5 psig\u4e0b\u4e00\u8d77\u9032\u884c\u58d3\u529b\u6e2c\u8a66\uff0c\u4ee5\u4fbf\u5b89\u5168\u904b\u8f38\u7a7a\u6c23\uff0c\u4e26\u53ef\u4ee513,000g\u96e2\u5fc3\u3002 \u5e3d\u5b50\u55ae\u7368\u51fa\u552e\uff08BV\uff03M1353\uff09<\/p>\n

\"\"<\/p>\n

\u4e7e\u6d74\u52a0\u71b1\u6c34\u6d74\u69fd<\/a>\u00a0|\u00a0Finnpipette F3 \u5fae\u91cf\u5206\u6ce8\u5668<\/a>\u00a0|\u00a0\u8a66\u7ba1\u632f\u76ea\u5668 Vortex Mixer\u00a0<\/a>\u00a0|\u00a0Genereach\u8ff7\u4f60\u578b\u5fae\u91cf\u96e2\u5fc3\u6a5f<\/a>\u00a0 |\u00a0\u00a0ELISA\u5100\u5668<\/a><\/span><\/strong><\/b><\/strong><\/span><\/p>\n

\"smart\"<\/a>\"Spectrumlab<\/a>\"102-T\"<\/a>\"0100-01\"<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\u7e3d\u81a0\u539f\u542b\u91cf\u6e2c\u5b9a\u8a66\u5291\u76d2\uff08\u7121\u9ad8\u6c2f\u9178\u9e7d\uff09\uff5c\u8ca8\u865fK406-100\u00a0 (abcam ab222942) Total Co […]<\/p>\n","protected":false},"author":3,"featured_media":28510,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[434],"tags":[2723],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/16673"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=16673"}],"version-history":[{"count":7,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/16673\/revisions"}],"predecessor-version":[{"id":52949,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/16673\/revisions\/52949"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/28510"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=16673"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=16673"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=16673"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}