{"id":16657,"date":"2018-05-25T15:09:57","date_gmt":"2018-05-25T07:09:57","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=16657"},"modified":"2022-07-20T16:42:03","modified_gmt":"2022-07-20T08:42:03","slug":"%e4%b8%99%e9%85%ae%e9%85%b8%e8%84%ab%e6%b0%ab%e9%85%b6%ef%bc%88pdh%ef%bc%89%e6%b4%bb%e6%80%a7%e6%aa%a2%e6%b8%ac-pyruvate-dehydrogenase-pdh-activity-colorimetric-assay-kit%e8%b2%a8%e8%99%9fk679-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=16657","title":{"rendered":"\u4e19\u916e\u9178\u812b\u6c2b\u9176\uff08PDH\uff09\u6d3b\u6027\u6aa2\u6e2c | Pyruvate Dehydrogenase (PDH) Activity Colorimetric Assay Kit\u8ca8\u865fK679-100"},"content":{"rendered":"

\u4e19\u916e\u9178\u812b\u6c2b\u9176\uff08PDH\uff09\u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2 <\/strong>| <\/strong>\u8ca8\u865f<\/strong>K679-100<\/a><\/span><\/p>\n

Pyruvate Dehydrogenase (PDH) Activity Colorimetric Assay Kit<\/em><\/strong><\/p>\n

\u7522\u54c1<\/strong>\u63cf\u8ff0\uff1a<\/strong>\u4e19\u916e\u9178\u812b\u6c2b\u9176\uff08Pyruvate Dehydrogenase, PDH\uff09\uff08EC 1.2.4.1\uff09\u5728\u78b3\u6c34\u5316\u5408\u7269\u4ee3\u8b1d (carbohydrate metabolism) \u4e2d\u5177\u6709\u91cd\u8981\u4f5c\u7528\u3002\u5b83\u8207\u4e8c\u6c2b\u786b\u8f9b\u9170\u8f49\u4e59\u9170\u9176\uff08dihydrolipoyl transacetylase, E2\uff09\u548c\u4e8c\u6c2b\u786b\u8f9b\u9170\u812b\u6c2b\u9176\uff08\u00a0dihydrolipoyl dehydrogenase, E3\uff09\u5f62\u6210\u4e86\u4e00\u500b\u7279\u5fb5\u660e\u78ba\u7684\u9176\u8907\u5408\u7269\u3002 PDH\u5728NAD\u548cCoA\u5b58\u5728\u4e0b\u5c07\u4e19\u916e\u9178\u8f49\u5316\u70ba\u4e59\u9170-CoA\uff0c\u4e26\u5c07glycolysis\u00a0\u7cd6\u9175\u89e3\u8207\u00a0citric acid cycle\u6ab8\u6aac\u9178\u5faa\u74b0\u806f\u7e6b\u8d77\u4f86\u3002 PDH\u6d3b\u6027\u53d7ATP \/ ADP\uff0cNADH \/ NAD\u6216\u4e59\u9170\u8f14\u9176A \/\u8f14\u9176A\u7684\u9ad8\u7d30\u80de\u5167\u6bd4\u7387\u7684\u6291\u5236\u3002\u5728\u4eba\u985e\u4e2d\uff0cPDH\u7f3a\u4e4f\u6703\u964d\u4f4e\u7dda\u7c92\u9ad4\u529f\u80fd\uff0c\u4e26\u8207\u795e\u7d93\u9000\u884c\u6027\u75be\u200b\u200b\u75c5 (\u00a0neurodegenerative diseases)\u6709\u95dc\u3002 PDH\u7f3a\u9677\u662fX\u9023\u9396\u7684;\u5b83\u5c0e\u81f42\u7a2e\u5f62\u5f0f\u7684\u7570\u5e38\uff1a\u4ee3\u8b1d\u5f62\u5f0f\uff08lactic acidosis\u4e73\u9178\u9178\u4e2d\u6bd2\uff09\u548c\u795e\u7d93\u5f62\u5f0f\uff08seizure\u7672\u7647\u767c\u4f5c\u548c\/\u6216neuropathological spasm\u795e\u7d93\u75c5\u7406\u6027\u75d9\u6523\uff09\u3002\u6700\u8fd1\u7684\u7814\u7a76\u8868\u660e\uff0cPDH\u662f\u81f4\u764c\u57fa\u56e0\u8a98\u5c0e\u7684\u8870\u8001\u7684\u9776\u6a19; PDH\u7684\u6fc0\u6d3b\u589e\u5f37\u4e19\u916e\u9178\u5229\u7528\u4e26\u589e\u52a0\u547c\u5438\u548c\u6c27\u5316\u9084\u539f\u61c9\u6fc0\u3002 BioVision\u7684PDH\u6aa2\u6e2c\u8a66\u5291\u76d2\u70ba\u76e3\u6e2c\u5404\u7a2e\u6a23\u54c1\u4e2d\u7684PDH\u6d3b\u6027\u63d0\u4f9b\u4e86\u4e00\u7a2e\u5feb\u901f\u7c21\u55ae\u7684\u65b9\u6cd5\u3002\u5728\u8a72\u6e2c\u5b9a\u4e2d\uff0cPDH\u5c07\u4e19\u916e\u9178\u8f49\u5316\u6210\u4e2d\u9593\u9ad4\uff0c\u5176\u5c07\u986f\u8272\u5291\u9084\u539f\u6210\u5728450nm\u8655\u5177\u6709\u5f37\u5438\u5149\u5ea6\u7684\u6709\u8272\u7522\u7269\u3002\u8a72\u6aa2\u6e2c\u65b9\u6cd5\u7c21\u55ae\uff0c\u9748\u654f\uff0c\u53ef\u5728\u5404\u7a2e\u6a23\u54c1\u4e2d\u6aa2\u6e2c\u5230\u4f4e\u65bc0.1 mU\u7684\u4e19\u916e\u9178\u812b\u6c2b\u9176\u6d3b\u6027\u3002<\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK679-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (450 nm)<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nMeasurement of pyruvate dehydrogenase activity in various tissues\/cells
\nAnalysis of cell signaling pathway<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple, rapid & convenient
\n\u2022 can measure pyruvate dehydrogenase activity lower than 0.1 mU in a variety of samples<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 PDH Assay Buffer
\n\u2022 PDH Substrate (Lyophilized)
\n\u2022 PDH Developer (Lyophilized)
\n\u2022 NADH Standard (Lyophilized)
\n\u2022 PDH Positive Control<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5f15\u7528\u6587\u737b<\/strong><\/p>\n

\n
Consitt, Leslie A. et al. (2016) Age-related impairments in skeletal muscle PDH phosphorylation and plasma lactate are indicative of metabolic inflexibility and the effects of exercise training.\u00a0Am J Physiol Endocrinol Metab. 2016 Jul 1;311(1):E145-56.<\/a><\/div>\n<\/div>\n
\n
Luz, Anthony L. et al. (2016) From the Cover: Arsenite Uncouples Mitochondrial Respiration and Induces a Warburg-like Effect in Caenorhabditis elegans.\u00a0Toxicol Sci. 2016 Aug;152(2):349-62.<\/a><\/div>\n<\/div>\n
\n
Sin et al., Acute Treatment of Resveratrol Alleviates Doxorubicin-Induced Myotoxicity in Aged Skeletal Muscle Through SIRT1-Dependent Mechanisms. \u00a0J Gerontol A Biol Sci Med Sci,\u00a0Oct 2015; 10.1093\/gerona\/glv175.<\/a><\/div>\n<\/div>\n
\n
Hagve et al., Skeletal muscle mitochondria exhibit decreased pyruvate oxidation capacity and increased ROS emission during surgery-induced acute insulin resistance.\u00a0Am J Physiol Endocrinol Metab,\u00a0Apr 2015; 308: E613 – E620.<\/a><\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

\u4e19\u916e\u9178\u812b\u6c2b\u9176\uff08PDH\uff09\u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2 | \u8ca8\u865fK679-100 Pyruvate Dehydrogenas […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[2724],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/16657"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=16657"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/16657\/revisions"}],"predecessor-version":[{"id":19262,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/16657\/revisions\/19262"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=16657"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=16657"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=16657"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}