{"id":16613,"date":"2018-05-22T11:57:34","date_gmt":"2018-05-22T03:57:34","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=16613"},"modified":"2022-07-20T16:43:14","modified_gmt":"2022-07-20T08:43:14","slug":"%e7%90%a5%e7%8f%80%e9%85%b8%e8%84%ab%e6%b0%ab%e9%85%b6%e6%b4%bb%e6%80%a7%e6%af%94%e8%89%b2%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92-succinate-dehydrogenase-activity-colorimetric-assay-kit","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=16613","title":{"rendered":"\u7425\u73c0\u9178\u812b\u6c2b\u9176\u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2 | Succinate Dehydrogenase Activity Colorimetric Assay Kit\u8ca8\u865fK660-100"},"content":{"rendered":"

\u7425\u73c0\u9178\u812b\u6c2b\u9176\u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2\uff08<\/strong>OD = 600 nm<\/strong>\uff09 <\/strong>| <\/strong>\u8ca8\u865f<\/strong>K660-100<\/a><\/span><\/p>\n

Succinate Dehydrogenase Activity Colorimetric Assay Kit<\/em><\/strong><\/p>\n

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\u7522\u54c1<\/strong>\u63cf\u8ff0\uff1a<\/strong>\u7425\u73c0\u9178\u812b\u6c2b\u9176\uff08Succinate Dehydrogenase, SDH\uff09\uff08EC 1.3.5.1\uff09\u6216\u7425\u73c0\u9178\u8f14\u9176Q\u9084\u539f\u9176\uff08succinate-coenzyme Q reductase, SQR\uff09\u6216\u547c\u5438\u8907\u5408\u7269II ( respiratory complex II is an enzyme complex)\u662f\u4e00\u7a2e\u9176\u8907\u5408\u7269\uff0c\u5b83\u8207\u5167\u7dda\u7c92\u9ad4\u819c(mitochondrial membrane)\u7d50\u5408\u3002 SDH\u53c3\u8207\u6ab8\u6aac\u9178\u5faa\u74b0(citric acid cycle)\u548c\u96fb\u5b50\u50b3\u8f38\u93c8(electron transport chain)\u3002\u5728\u54fa\u4e73\u52d5\u7269(mammals )\u548c\u8a31\u591a\u7d30\u83cc(bacteria)\u4e2d\uff0cSDH\u75312\u500b\u89aa\u6c34\u6027\u4e9e\u57fahydrophilic subunits\u7d44\u6210\uff0cSDHA\uff08\u9ec3\u7d20\u86cb\u767d\uff09\u548cSDHB\uff08\u9435\u786b\u86cb\u767d\uff09\u4ee5\u53ca2\u500b\u758f\u6c34\u6027\u819c\u9328\u5b9a\u4e9e\u57fa\uff1aSDHC\u548cSDHD\u3002 SDH\u5c07succinate \u7425\u73c0\u9178\u6c27\u5316\u6210\u5bcc\u99ac\u9178(fumarate)<\/span>\u916f\u4e26\u5c07\u96fb\u5b50\u8f49\u79fb\u5230\u6cdb\u918c(ubiquinone)<\/span>\u3002\u4eba\u985eSDH\u7f3a\u9677\u5c0e\u81f4\u591a\u7a2e\u8868\u73fe\u578b\uff0c\u5305\u62ecLeigh\u7d9c\u5408\u5fb5\u72c0(Leigh syndrome)\uff0c\u795e\u7d93\u4ee3\u8b1d\u7d0a\u4e82(neurometabolic disorder)\uff0c\u816b\u7624\u5f62\u6210(tumor formation)\u548c\u808c\u75c5(myopathy)\u3002\u6700\u8fd1\u7684\u7814\u7a76\u8868\u660eSDH\u53ef\u4ee5\u963b\u6b62ROS\uff08\u6d3b\u6027\u6c27\uff09\u7684\u7522\u751f; \u56e0\u6b64\uff0c\u7425\u73c0\u9178\u812b\u6c2b\u9176(Succinate Dehydrogenas)\u6d3b\u6027\u7684\u6e2c\u91cf\u5177\u6709\u5ee3\u6cdb\u7684\u61c9\u7528\u3002 BioVision\u7684\u7425\u73c0\u9178\u812b\u6c2b\u9176(Succinate Dehydrogenas)\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2\u5177\u6709\u5feb\u901f\uff0c\u7c21\u55ae\u548c\u9ad8\u901a\u91cf\u9069\u61c9\u6027\u3002\u5728\u8a72\u6e2c\u5b9a\u4e2d\uff0c\u7425\u73c0\u9178\u812b\u6c2b\u9176(Succinate Dehydrogenas)\u5c07\u7425\u73c0\u9178\u8f49\u5316\u70ba\u5bcc\u99ac\u9178(fumarate)\uff0c\u4e26\u5c07\u96fb\u5b50\u8f49\u79fb\u81f3\u4eba\u9020\u96fb\u5b50\u53d7\u9ad4(artificial electron acceptor)\uff08\u63a2\u91dd\uff09\uff0c\u5176\u5c07\u984f\u8272\u5f9e\u85cd\u8272\u8b8a\u70ba\u7121\u8272\u7522\u7269\uff08\u53d6\u6c7a\u65bc\u6a23\u54c1\u7684\u9176\u6d3b\u6027\uff09\u3002\u8a72\u6aa2\u6e2c\u8a66\u5291\u76d2\u53ef\u5728\u5404\u7a2e\u6a23\u54c1\u4e2d\u6aa2\u6e2c\u5230\u5c0f\u65bc0.1mU\u7684\u7425\u73c0\u9178\u812b\u6c2b\u9176\u6d3b\u6027\u3002<\/p>\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK660-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (OD = 600 nm)<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nMeasurement of Succinate Dehydrogenase Activity in various tissues\/cells.
\nAnalysis of citric acid cycle<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple & rapid
\n\u2022 High-throughput adaptable
\n\u2022 This assay kit can detect less than 0.1mU Succinate Dehydrogenase Activity in a variety of samples.<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 SDH Assay Buffer
\n\u2022 SDH Substrate Mix (Lyophilized)
\n\u2022 SDH Probe
\n\u2022 DCIP Standard (2 mM)
\n\u2022 SDH Positive Control (Lyophilized)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5f15\u7528\u6587\u737b<\/p>\n

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Maricela Robles-Murguia, Tissue-specific alteration of gene expression and function by RU486 and the GeneSwitch system. NPJ Aging Mech Dis, May 2019; PMC6529481.<\/a><\/div>\n<\/div>\n
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Min Guo, et al., (2018) Ketogenic Diet Improves Brain Ischemic Tolerance and Inhibits NLRP3 Inflammasome Activation by Preventing Drp1-Mediated Mitochondrial Fission and Endoplasmic Reticulum Stress, Frontiers in Molecular Neuroscience, Mar. 2018, 29662437<\/a><\/div>\n<\/div>\n
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Wenliang Zhang, et al., (2018) Adipose-specific lipin1 overexpression in mice protects against alcohol-induced liver injury, Scientific Reports, Jan. 2018, 29323242<\/a><\/div>\n<\/div>\n

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\u7425\u73c0\u9178\u812b\u6c2b\u9176\u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2\uff08OD = 600 nm\uff09 | \u8ca8\u865fK660-100 Succinate Deh […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[1],"tags":[2733],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/16613"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=16613"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/16613\/revisions"}],"predecessor-version":[{"id":25257,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/16613\/revisions\/25257"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=16613"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=16613"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=16613"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}