{"id":16604,"date":"2018-05-21T10:42:25","date_gmt":"2018-05-21T02:42:25","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=16604"},"modified":"2022-07-20T16:43:33","modified_gmt":"2022-07-20T08:43:33","slug":"lightning-link-hrp-labeling-kit-hrp%e6%8a%97%e9%ab%94%e8%9b%8b%e7%99%bd%e8%b3%aa%e6%a8%99%e8%aa%8c%e5%a5%97%e7%b5%84-expendeon%e8%b2%a8%e8%99%9f701-0000","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=16604","title":{"rendered":"Lightning-Link\u00ae HRP Labeling kit| HRP\u6297\u9ad4\u86cb\u767d\u8cea\u6a19\u8a8c\u5957\u7d44 Expendeon\u8ca8\u865f701-0000"},"content":{"rendered":"<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=16604\"><strong>Lightning-Link\u00ae Horseradish Peroxidase (HRP)<\/strong>\u00a0<strong>|\u00a0<\/strong><strong>\u6297\u9ad4<\/strong><strong>\u86cb\u767d\u8cea<\/strong><strong>\u6a19\u8a8c\u5957\u7d44<\/strong>\u00a0<\/a><\/span><span style=\"color: #3366ff;\"><strong>\u8ca8\u865f<\/strong><strong>#701-0000<\/strong><strong>\u5305\u88dd<\/strong><strong>3x100UG<\/strong><\/span><\/p>\n<p><span style=\"color: #000000;\">\u53ea\u9700\u4e00\u500b\u6b65\u9a5f\u4e0d\u9808Dialysis\u6216desalt\u6b65\u9a5f\u3002\u6297\u9ad4\u6a19\u8a8c\u6548\u80fd\u8fd1\u4e4e100%\u3002<\/span><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/www.innovabiosciences.com\/images\/stories\/llrapiddylight.jpg\" alt=\"\" width=\"904\" height=\"127\" \/><\/p>\n<p><strong>\u985e\u5225:HRP\u9175\u7d20\u00a0\u6297\u9ad4\/\u86cb\u767d\u8cea\/\u80dc\u80bd\uff08amino group\uff09\u6a19\u8a8c<\/strong><\/p>\n<p><strong>\u7522\u54c1\u654d\u8ff0:<\/strong>\u00a0HRP\u9175\u7d20\u6297\u9ad4\u6a19\u8a8cdirectly labeling kit (\u8ca8\u865f#701-0000)\uff0c\u9996\u9078Expendeon\u516c\u53f8\u5c08\u5229\u7814\u767c\u4e4blightning-link\u6297\u9ad4\u87a2\u5149\u6a19\u8a8c\u5957\u7d44\u3002\u5e02\u9762\u4e0aantibody labeling kit\uff0c\u5728pH8~9\u74b0\u5883\u4e0b\u505a\u53cd\u61c9\u3002\u5982\u6b64\u5730\u74b0\u5883\u6613\u9020\u6210\u6297\u9ad4\u6d3b\u6027\u5931\u6548\uff0c\u5373\u4f7f\u7167\u8457\u539f\u5ee0\u63d0\u4f9b\u7684Protocol\u4e0b\u9032\u884c\u53cd\u61c9\uff0c\u6297\u9ad4\u56de\u6536\u7684\u6548\u80fd\u4e5f\u5f88\u5dee; \u592a\u591a\u6297\u9ad4\u672a\u88ab\u6a19\u8a8c\uff0c\u6240\u4ee5\u5fc5\u9808\u7d93\u7531Dialysis\u53cadesalt\u6b65\u9a5f\u79fb\u53bb\u672a\u7d50\u5408\u7684\u6297\u9ad4\u3002\u5982\u6b64\u4e00\u4f86\u6d6a\u8cbb\u4e86\u73cd\u8cb4\u7684\u6297\u9ad4, \u6297\u9ad4\u7d50\u5408\u6548\u80fd\u4e5f\u4e0d\u7406\u60f3\u3002Lightning-link\u5546\u54c1\u514b\u670d\u6297\u9ad4\u6a19\u8a8c\u6548\u80fd\u4e0d\u7406\u60f3\u7684\u554f\u984c\uff0c\u5c08\u5229\u7814\u767c\u6539\u826f\uff0c\u6297\u9ad4\u6a19\u8a8c\u6548\u80fd\u8fd1\u4e4e100%\uff0c\u662f\u6a19\u8a8c\u7684\u9996\u9078\u5546\u54c1\u3002\u6a19\u8a8c\u6297\u9ad4\u7bc4\u570d10ug-1mg\uff0c\u5927\u91cf\u6a19\u8a8c\u8acb\u4f86\u96fb\u6d3d\u8a62<a href=\"http:\/\/biopioneer.com.tw\/\">02-86609496<\/a><\/p>\n<p><img decoding=\"async\" src=\"https:\/\/www.expedeon.com\/wp-content\/uploads\/2017\/08\/Data-Lightning-Link-HRP-western-blot-GFAP.jpg\" alt=\"Primary antibody directly conjugated to HRP using Lightning-Link\" \/><\/p>\n<p><strong>\u751f\u7269\u6a19\u8a8c\u7684\u5c08\u5bb6Expendeon<\/strong><\/p>\n<p>Expendeon\u4ee5\u751f\u7269\u7d50\u5408\u70ba\u4e3b\u8981\u4e8b\u696d\u3002\u65bc1996\u5e74\u5c08\u5229\u7814\u767c\uff0c\u4e2d\u6027\u74b0\u5883\u4e0b\u9032\u884c\u6297\u9ad4\u7d50\u5408\u53cd\u61c9\uff0c\u53ea\u8981\u4e00\u500b\u6b65\u9a5f\uff0c30\u79d2hands on time\u3002\u5176\u6297\u9ad4\u56de\u6536\u6548\u80fd\u8fd1\u4f3c100%\u3002\u4e0d\u9808\u518d\u505aDialysis\u6216desalt\u7684\u6b65\u9a5f\u3002Lightning-Link \u53ea\u8981\u4e00\u500b\u6b65\u9a5f\uff0c\u5373\u53ef\u5c07\u86cb\u767d\u8cea\/\u6297\u9ad4\/\u80dc\u80bd\u505a\u6a19\u8a8c\uff0c\u5177\u5c08\u5229\u7814\u767cQuencher reagent\u5316\u5b78\u53cd\u61c9\u5fb9\u5e95\u4e2d\u6b62\u6b98\u9918\u53cd\u61c9\u53ca\u505c\u6b62\u975e\u5c08\u4e00\u6027\u7d50\u5408\uff0c\u56e0\u6b64\u7121\u9808desalt\u53cadialysis\uff0c\u6297\u9ad4\u6a19\u8a8c\u56de\u6536\u7387100%\u3002<\/p>\n<p><iframe loading=\"lazy\" src=\"\/\/www.youtube.com\/embed\/oFt1PtkCvb8\" width=\"425\" height=\"350\" allowfullscreen=\"allowfullscreen\"><\/iframe><\/p>\n<p><strong>Lightning-link<\/strong><strong>\u6297\u9ad4\u6a19\u8a8c\u539f\u7406<\/strong><\/p>\n<p>Lightning-Link\u4ee5\u5171\u50f9\u9375\u7d50\uff0c\u8fa8\u8996amino groups\uff0c\u6027\u8cea\u6975\u70ba\u7a69\u5b9a\uff0c\u4e00\u6b21\u53cd\u61c9\u5f8c\uff0c\u53ef\u51b7\u51cd\u4fdd\u5b58\uff0c\u7bc0\u7701\u5be6\u9a57\u6642\u9593\u3002<img decoding=\"async\" src=\"https:\/\/www.innovabiosciences.com\/components\/com_virtuemart\/shop_image\/product\/resized\/Horse_Radish_Per_4c93401f701f0_360x360.png\" alt=\"\" \/><\/p>\n<p><img decoding=\"async\" src=\"https:\/\/www.expedeon.com\/wp-content\/uploads\/2017\/08\/Lightning-Link_process_diagram_400w.png\" alt=\"Lightning-Link_process_diagram\" \/><\/p>\n<p>Horseradish peroxidase is a 44kDa glycoprotein with 6 lysine residues. The enzyme label can be visualized by chromogenic reactions; for example diaminobenzidine (DAB) in the presence of hydrogen peroxide (H<sub>2<\/sub>0<sub>2<\/sub>) is converted in to a water insoluble brown pigment. Other substrates which can be used to measure horseradish peroxidase activity include ABTS, TMB and TMBUS.<\/p>\n<p><strong>Lightning-link<\/strong><strong>\u6297\u9ad4\u6a19\u8a8c\u61c9\u7528<\/strong><\/p>\n<p>\u53ef\u4ee5\u61c9\u7528\u5728ELISA. immunofluorescent. Flow. confocol. Western Blot\u7b49\u6aa2\u8a3a\u53ca\u5206\u5b50\u75c5\u6bd2\u4e4b\u7814\u7a76!!\u76f4\u63a5\u5728\u4e00\u6297\u4e0a\u505a\u6a19\u8a8c (\u53ef\u6a19\u8a8c\u87a2\u5149\u86cb\u767d \u87a2\u5149dye HRP Biotin\u7b49) \uff0c\u514b\u670d\u4e8c\u6297\u6240\u9020\u6210\u7684\u80cc\u666f\u503c\u904e\u9ad8\u53caHost\u4f86\u6e90\u4e0d\u540c\u6240\u9020\u6210Crosstalk\u7684\u73fe\u8c61\u3002<strong>Lightning-link\u6297\u9ad4\u6a19\u8a8cV.S.\u4fdd\u5b58\u6db2\u00a0<\/strong>\u6297\u9ad4\u4fdd\u5b58\u65bcAzide\u3001Gelatin\u53caBSA\u6eb6\u6db2\u4e0b\uff0c\u6703\u5e72\u64fe\u4e00\u822c\u7d50\u5408\u7684\u6548\u679c\uff0c\u6240\u4ee5\u6703\u518d\u7d93\u904e\u7d14\u5316\u6b65\u9a5f\uff0c\u6d6a\u8cbb\u8a31\u591a\u6297\u9ad4\u3002Lightning-Link\u514b\u670dAzide\u3001Gelatin\u53caBSA\u5e72\u64fe\u7d50\u5408\u7684\u6548\u7387\uff0c\u7701\u4e0b\u60a8\u5bf6\u8cb4\u7684\u6297\u9ad4\u3002\u5225\u65bc\u5176\u5b83\u7d50\u5408\u7684\u65b9\u5f0f\uff0cLightning-Link\u7121\u9808\u5728High pH\u7684\u6eb6\u6db2\u4e0b\u64cd\u4f5c\uff0c\u6240\u4ee5\u53ea\u8981\u662f\u5177\u6709amino groups (lysin\/a-amino)\u7684\u86cb\u767d\u8cea\/\u80dc\u80bd\/\u6297\u9ad4\uff0c\u90fd\u53ef\u53cd\u61c9\u3002<\/p>\n<p><strong><span style=\"color: #ff0000;\">\u66f4\u591a\u6297\u9ad4\u6a19\u8a8c\u76f8\u95dc<\/span><\/strong>\u00a0<strong><span style=\"color: #3366ff;\">\u00a0<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=7284\">InnovaGold\u5948\u7c73\u91d1\u6a19\u8a8c\u5feb\u7be9\u8a66\u5291<\/a>\u00a0|\u00a0<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=7703\">Lightning-link\u9175\u7d20\u578b\u6297\u9ad4\u6a19\u8a8c<\/a>\u00a0|\u00a0<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=5295\">Abselect\u6297\u9ad4\u7d14\u5316\u00a0<\/a>|\u00a0<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=5351\">SBA\u55ae\u682a\u6297\u9ad4\u5206\u578bELISA<\/a>\u00a0|\u00a0<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=5283\">Lightning-link\u87a2\u5149\u86cb\u767d\u6297\u9ad4\u6a19\u8a8c<\/a>\u00a0|\u00a0<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=3480\">Thunder-link\u6838\u9178\u6297\u9ad4\u6a19\u8a8c<\/a><\/span><\/strong><\/p>\n<table border=\"1\" cellspacing=\"0\" cellpadding=\"0\">\n<tbody>\n<tr>\n<td style=\"text-align: center;\" valign=\"top\" nowrap=\"nowrap\" width=\"100\"><strong>\u7522\u54c1\u7de8\u865f<\/strong><\/td>\n<td style=\"text-align: center;\" valign=\"top\" nowrap=\"nowrap\" width=\"476\"><strong>\u7522\u54c1\u540d\u7a31<\/strong><\/td>\n<td style=\"text-align: center;\" valign=\"top\" nowrap=\"nowrap\" width=\"100\"><strong>\u5305\u88dd<\/strong><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" valign=\"top\" nowrap=\"nowrap\" width=\"76\">701-0000<\/td>\n<td valign=\"top\" nowrap=\"nowrap\" width=\"313\"><a href=\"https:\/\/www.innovabiosciences.com\/products\/antibody-protein-labeling\/lightning-link\/enzyme-labels\/lightning-link-horseradish-peroxidase\">Lightning-Link\u00ae Horseradish Peroxidase (HRP)<\/a><\/td>\n<td style=\"text-align: center;\" valign=\"top\" nowrap=\"nowrap\" width=\"72\">3&#215;100\u03bcg<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" valign=\"top\" nowrap=\"nowrap\" width=\"76\">702-0010<\/td>\n<td valign=\"top\" nowrap=\"nowrap\" width=\"313\"><a href=\"https:\/\/www.innovabiosciences.com\/products\/antibody-protein-labeling\/lightning-link\/enzyme-labels\/lightning-link-alkaline-phosphatase\">Lightning-Link\u00ae Alkaline Phosphatase (AP)<\/a><\/td>\n<td style=\"text-align: center;\" valign=\"top\" nowrap=\"nowrap\" width=\"72\">3&#215;100\u03bcg<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" valign=\"top\" nowrap=\"nowrap\" width=\"76\">706-0010<\/td>\n<td valign=\"top\" nowrap=\"nowrap\" width=\"313\"><a href=\"https:\/\/www.innovabiosciences.com\/products\/antibody-protein-labeling\/lightning-link\/enzyme-labels\/lightning-link-glucose-oxidase\">Lightning-Link\u00ae Glucose Oxidase<\/a><\/td>\n<td style=\"text-align: center;\" valign=\"top\" nowrap=\"nowrap\" width=\"72\">3&#215;100\u03bcg<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"https:\/\/www.expedeon.com\/wp-content\/uploads\/2017\/08\/Process-diagram-HRP-CheckGo.png\" alt=\"HRP Check&amp;Go! schema\" width=\"651\" height=\"242\" \/><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Lightning-Link\u00ae Horseradish Peroxidase (HRP)\u00a0|\u00a0\u6297\u9ad4\u86cb\u767d\u8cea\u6a19\u8a8c\u5957 [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":16605,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[195],"tags":[2735],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/16604"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=16604"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/16604\/revisions"}],"predecessor-version":[{"id":18849,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/16604\/revisions\/18849"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/16605"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=16604"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=16604"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=16604"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}