{"id":16500,"date":"2018-05-09T12:00:25","date_gmt":"2018-05-09T04:00:25","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=16500"},"modified":"2022-07-20T16:48:44","modified_gmt":"2022-07-20T08:48:44","slug":"maleimide%e6%b4%bb%e5%8c%96hrp%e6%8a%97%e9%ab%94%e6%a8%99%e8%aa%8c%e5%a5%97%e7%b5%84-expedeon%e8%b2%a8%e8%99%9f-401-0005","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=16500","title":{"rendered":"Maleimide\u6d3b\u5316HRP\u6297\u9ad4\u6a19\u8a8c\u5957\u7d44 |Expedeon\u8ca8\u865f 401-0005"},"content":{"rendered":"

Maleimide\u6d3b\u5316HRP\u6297\u9ad4\u6a19\u8a8c\u5957\u7d44<\/u><\/strong> |<\/strong>\u8ca8\u865f<\/strong> 401-0005<\/strong><\/a><\/span><\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> Expedeon Maleimide activated labels\u8a2d\u8a08Maleimide\u6d3b\u5316\u7684\u6a19\u8a18\uff0c\u4ee5\u4fc3\u9032\u6240\u9700\u6a19\u8a8c\uff0c\u53ef\u8207\u5305\u542b\u5df0\u57fa\uff08-SH\uff09\u57fa\u5718\u7684\u6297\u9ad4\uff0c\u86cb\u767d\u8cea\uff0c\u80bd\u6216\u914d\u9ad4\u9032\u884c\u7d50\u5408Covalent bond\u5171\u8edb\u53cd\u61c9\u3002 \u53ef\u65bc\u5728\u751f\u7406pH\u4e0b6.5-7.5\u7684pH\u7bc4\u570d\u5167\uff0c\u975e\u5e38\u6709\u6548\u5730\u9032\u884c\u7d50\u5408\u53cd\u61c9\u4ee5\u7372\u5f97\u512a\u7570\u7684\u6027\u80fd\u3002\u7522\u54c1\u6ce8\u610f\u4e8b\u9805: \u786b\u9187\u5316\u5206\u5b50\u6eb6\u89e3\u65bc\u5176\u4e2d\u7684\u7de9\u885d\u6db2\uff0c\u61c9\u8a72\u57286.5-7.5\u7684pH\u7bc4\u570d\u5167\u3002\u78f7\u9178\u9e7d\u7de9\u885d\uff0c\u901a\u5e38\u4f7f\u7528\u9e7d\u6c34pH 7.2\u3002 EDTA\uff081-10mM\uff09\u53ef\u80fd\u6709\u52a9\u65bc\u9632\u6b62\u786b\u9187\u7684\u91d1\u5c6c\u50ac\u5316\u6c27\u5316\u3002\u8acb\u53c3\u95b1\u63a8\u85a6\u7684\u7de9\u885d\u5340\u689d\u4ef6\u3002<\/p>\n

\"Maleimide<\/p>\n

\u57161.\u5df0\u57fa\u5316\u5c71\u7f8a\u6297\u5154IgG\u8207\u99ac\u4f86\u9170\u4e9e\u80fa\u6d3b\u5316\u7684\u9e7c\u6027\u78f7\u9178\u9176\u4e00\u8d77\u53cd\u61c9\u3002 \u4f7f\u7528\u5154IgG\u5857\u8986\u677f\u548c\u5c0d\u785d\u57fa\u82ef\u57fa\u78f7\u9178\u916f\uff08PNPP\uff09\u4f5c\u70ba\u9176\u5e95\u7269\uff0c\u901a\u904eELISA\u5206\u6790\u3002<\/p>\n

\"Maleimide<\/p>\n

\u57162.\u5df0\u57fa\u5316\u5c71\u7f8a\u6297\u5154IgG\u8207\u99ac\u4f86\u9170\u4e9e\u80fa\u6d3b\u5316\u7684HRP\u53cd\u61c9\u3002 \u4f7f\u7528\u5154IgG\u5857\u8986\u7684\u5e73\u677f\u548c2-2-\u6c2e\u96dc\u96d9\uff083-\u4e59\u57fa\u82ef\u4e26\u567b\u5511\u5549-6-\u78fa\u9178\uff1aABTS\uff09\u4f5c\u70baHRP\u9176\u5e95\u7269\uff0c\u901a\u904eELISA\u5206\u6790\u3002<\/p>\n

\"Maleimide<\/p>\n

\u57163.\u5df0\u57fa\u5316\u5c71\u7f8a\u6297\u5154IgG\u8207\u99ac\u4f86\u9170\u4e9e\u80fa\u6d3b\u5316\u7684\u93c8\u9ef4\u6297\u751f\u7269\u7d20\u86cb\u767d\u4e00\u8d77\u53cd\u61c9\u3002 \u4f7f\u7528\u5154IgG\u5305\u88ab\u7684ELISA\u677f\uff0c\u751f\u7269\u7d20\u5316\u7684HRP\u4e8c\u7d1a\u548cHRP\u5e95\u72692-2-\u5416\u55ea – \u96d9\uff083-\u4e59\u57fa\u82ef\u4e26\u567b\u5511\u5549-6-\u78fa\u9178\uff09\uff08ABTS\uff09\u901a\u904eELISA\u5206\u6790\u3002<\/p>\n

\"Maleimide<\/p>\n

Figure 4. Thiolated goat anti-rabbit IgG was incubated with maleimide-activated R-PE. The resulting conjugate was analysed by ELISA using a rabbit IgG coated plate.<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
401-0002<\/td>\nMaleimide labels – Maleimide-HRP (2mg)<\/td>\nMaleimide-HRP (2mg)<\/td>\n<\/tr>\n
401-0005<\/td>\nMaleimide labels – Maleimide-HRP (5mg)<\/td>\nMaleimide-HRP (5mg)<\/td>\n<\/tr>\n
402-0002<\/td>\nMaleimide labels – Maleimide-Alkaline Phosphatase (2mg)<\/td>\nMaleimide-Alkaline Phosphatase (2mg)<\/td>\n<\/tr>\n
402-0005<\/td>\nMaleimide labels – Maleimide Alkaline Phosphatase (5mg)<\/td>\nMaleimide Alkaline Phosphatase (5mg)<\/td>\n<\/tr>\n
403-0002<\/td>\nMaleimide labels – Maleimide-R Phycoerythrin (2mg)<\/td>\nMaleimide-R Phycoerythrin (2mg)<\/td>\n<\/tr>\n
403-0005<\/td>\nMaleimide labels – Maleimide-R Phycoerythrin (5mg)<\/td>\nMaleimide-R Phycoerythrin (5mg)<\/td>\n<\/tr>\n
404-0002<\/td>\nMaleimide labels – Maleimide-Allophycocyanin-XL (2mg)<\/td>\nMaleimide-Allophycocyanin-XL (2mg)<\/td>\n<\/tr>\n
405-0002<\/td>\nMaleimide labels – Maleimide-Streptavidin (2mg)<\/td>\nMaleimide-Streptavidin (2mg)<\/td>\n<\/tr>\n
405-0005<\/td>\nMaleimide labels – Maleimide-Streptavidin (5mg)<\/td>\nMaleimide-Streptavidin (5mg)<\/td>\n<\/tr>\n
407-0002<\/td>\nMaleimide labels – Maleimide-Ovalbumin (2mg)<\/td>\nMaleimide-Ovalbumin (2mg)<\/td>\n<\/tr>\n
407-0005<\/td>\nMaleimide labels – Maleimide-Ovalbumin (5mg)<\/td>\nMaleimide-Ovalbumin (5mg)<\/td>\n<\/tr>\n
408-0002<\/td>\nMaleimide labels – Maleimide-BSA (2mg)<\/td>\nMaleimide-BSA (2mg)<\/td>\n<\/tr>\n
408-0005<\/td>\nMaleimide labels – Maleimide-BSA (5mg)<\/td>\nMaleimide-BSA (5mg)<\/td>\n<\/tr>\n
409-0002<\/td>\nMaleimide labels – Maleimide-KLH (2mg)<\/td>\nMaleimide-KLH (2mg)<\/td>\n<\/tr>\n
409-0005<\/td>\nMaleimide labels – Maleimide-KLH (5mg)<\/td>\nMaleimide-KLH (5mg)<\/td>\n<\/tr>\n
418-0002<\/td>\nThiol kits – Thiol quantification kit (192 assays)<\/td>\nThiol quantification kit (192 assays)<\/td>\n<\/tr>\n
419-0002<\/td>\nThiol kits – Protein Thiolation Kit (2mg)<\/td>\nProtein Thiolation Kit (2mg)<\/td>\n<\/tr>\n
419-0005<\/td>\nThiol kits – Protein Thiolation kit (5mg)<\/td>\nProtein Thiolation kit (5mg)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u71b1\u9580\u5546\u54c1<\/span><\/strong> 400nm\u4e73\u81a0\u73e0\u6297\u9ad4\u6a19\u8a8c\u5957\u7d44 (\u7d05\u8272)<\/a> | 400nm\u4e73\u81a0\u73e0\u6297\u9ad4\u6a19\u8a8c\u5957\u7d44 (\u85cd\u8272) <\/a>| 400nm\u4e73\u81a0\u73e0\u6297\u9ad4\u6a19\u8a8c\u5957\u7d44 (\u9ed1\u8272)<\/a>\u00a0<\/span>\u76f8\u95dc\u5546\u54c1<\/span><\/strong> Streptavidin-gold\u5948\u7c73\u91d1\u7c92\u5b50(40nm)<\/a> | Streptavidin-gold\u5948\u7c73\u91d1\u7c92\u5b50(20nm)<\/a> | Biotin-gold\u5948\u7c73\u91d1\u7c92\u5b50(40nm)<\/a> | 10-80nm\u5948\u7c73\u91d1\u6297\u9ad4\u6a19\u8a8c\u5957\u7d44<\/a> | \u5948\u7c73\u91d1\u6eb6\u6db2<\/a><\/span><\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Maleimide\u6d3b\u5316HRP\u6297\u9ad4\u6a19\u8a8c\u5957\u7d44 |\u8ca8\u865f 401-0005 \u7522\u54c1\u4ecb\u7d39: Expedeon Maleim […]<\/p>\n","protected":false},"author":3,"featured_media":16501,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[2751],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/16500"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=16500"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/16500\/revisions"}],"predecessor-version":[{"id":16505,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/16500\/revisions\/16505"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/16501"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=16500"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=16500"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=16500"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}