{"id":16432,"date":"2018-04-26T10:52:45","date_gmt":"2018-04-26T02:52:45","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=16432"},"modified":"2025-03-19T10:00:34","modified_gmt":"2025-03-19T02:00:34","slug":"m-h%e5%9f%b9%e9%a4%8a%e5%9f%bamuller-hinton-agar%e8%b2%a8%e8%99%9flab114","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=16432","title":{"rendered":"M-H\u57f9\u990a\u57fa |Mueller-Hinton Broth\u8ca8\u865fLAB114"},"content":{"rendered":"

M-H\u57f9\u990a\u57fa\uff08Mueller-Hinton broth\uff09<\/strong>|<\/strong>\u8ca8\u865f<\/strong>LAB114<\/strong><\/a>\u00a0<\/span><\/p>\n

An antagonist-free medium for use in the tube dilution technique for the determination of antibiotic M.I.C. values<\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> M-H\u57f9\u990a\u57fa\uff08Mueller-Hinton Broth\uff09\u662f\u4e00\u7a2e\u5fae\u751f\u7269\u57f9\u990a\u57fa\uff0c\u61c9\u7528\u65bc\u6aa2\u6e2c\u5fae\u751f\u7269\u5c0d\u65bc\u6297\u751f\u7d20\u7684\u6297\u6027\u3002\u6b64\u5916\uff0c\u9019\u7a2e\u57f9\u990a\u57fa\u9084\u53ef\u7528\u65bc\u7be9\u9078\u5948\u745f\u6c0f\u83cc\u5c6c\uff08Neisseria\uff09\u4ee5\u53caMoraxella\u5c6c\u7684\u7269\u7a2e\u3002\u7531\u65bc\u8a72\u57f9\u990a\u57fa\u7684\u4e00\u4e9b\u7279\u6027\uff0c\u4f7f\u5b83\u6709\u5229\u65bc\u61c9\u7528\u5728\u6297\u751f\u7d20\u6aa2\u6e2c\u4e0a\u3002\u9996\u5148\uff0c\u5b83\u662f\u4e00\u7a2e\u7121\u9078\u64c7\u6027\u57f9\u990a\u57fa\uff0c\u5927\u90e8\u5206\u7684\u5fae\u751f\u7269\u90fd\u53ef\u5728\u4e0a\u9762\u751f\u9577\u3002\u6b64\u5916\uff0c\u6210\u5206\u4e2d\u7684\u6fb1\u7c89\u53ef\u4ee5\u5438\u6536\u7d30\u83cc\u6240\u91cb\u653e\u51fa\u4f86\u7684\u6bd2\u7d20\uff0c\u56e0\u6b64\u4e0d\u6703\u5f71\u97ff\u6297\u751f\u7d20\u64cd\u4f5c\u7684\u7d50\u679c\u3002M-H\u57f9\u990a\u57fa\u7684\u6210\u5206\u8f03\u70ba\u758f\u9b06\uff0c\u5229\u65bc\u6297\u751f\u7d20\u7684\u64f4\u6563\uff0c\u4f7f\u5176\u53ef\u4ee5\u986f\u73fe\u660e\u986f\u7684\u751f\u9577\u6291\u5236\u5e36\u6291\u83cc\u5708<\/em>Zone of inhibition\uff08zone of inhibition\uff09\u3002This medium is the broth version of Mueller Hinton Agar. It is an antagonist free medium for use in the tube dilution technique for the determination of antibiotic M.I.C. values. The medium is carefully standardised to meet N.C.C.L.S. standards for antimicrobial susceptibility tests on bacteria which grow aerobically.<\/p>\n

\u57f9\u990a\u57fa\u4f7f\u7528\u65b9\u6cd5<\/strong>: <\/strong>\u7a31\u53d621\u514b\u7c89\u672b\uff0c\u5206\u6563\u65bc1\u5347\u84b8\u993e\u6c34\u4e2d\u3002 \u5141\u8a31\u6d78\u6ce110\u5206\u9418\uff0c\u65cb\u8f49\u81f3\u6df7\u5408\u7136\u5f8c\u8f15\u8f15\u52a0\u71b1\u6eb6\u89e3\u3002\u5206\u914d\u5230\u7ba1\u5b50\u6216\u74f6\u5b50\u4e2d\uff0c\u4e26\u5728121\u00b0C\u6ec5\u83cc10\u6b21\u5206\u9418\u3002\u5132\u5b58\u65b9\u5f0f10-25\u00b0C\u3002\u6e96\u5099\u597d\u7684Medium\uff1a\u5728\u9ed1\u6697\u4e2d2-8\u00b0C 7\u5929\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n
\u57f9\u990a\u57fa<\/td>\n\u6e2c\u8a66\u83cc\u985e<\/td>\n<\/tr>\n
Trypticase soy broth<\/td>\n\u7d30\u83cc<\/td>\n<\/tr>\n
Brain heart infusion broth<\/td>\n\u7d30\u83cc<\/td>\n<\/tr>\n
Blood agar plate<\/td>\n\u7d30\u83cc\u8207\u53ad\u6c27\u83cc<\/td>\n<\/tr>\n
Thioglycollate medium<\/td>\n\u53ad\u6c27\u83cc<\/td>\n<\/tr>\n
Sabouraud dextrose broth<\/td>\n\u9175\u6bcd\u83cc\u8207\u9ef4\u83cc<\/td>\n<\/tr>\n
YM broth<\/td>\n\u9175\u6bcd\u83cc\u8207\u9ef4\u83cc<\/td>\n<\/tr>\n
Nutrient broth with 2% yeast extract<\/td>\n\u7d30\u83cc,\u9175\u6bcd\u83cc\u8207\u9ef4\u83cc<\/td>\n<\/tr>\n
YM broth<\/td>\n\u9175\u6bcd\u83cc\u8207\u9ef4\u83cc<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

LAB M LB<\/strong>\u7c89\u672b\u57f9\u990a\u57fa<\/strong> |<\/strong> LB agar\/Broth<\/strong>\u57f9\u990a\u57fa<\/strong><\/p>\n

LAB M\u4f4d\u65bc\u82f1\u570b\uff0c\u662f\u57f9\u990a\u57fa\u8001\u54c1\u724c\u516c\u53f8\uff0c\u53e3\u7891\u597d\u54c1\u8cea\u4f73\uff0c\u63a1\u7528\u5bc6\u5c01\u5c01\u53e3\u819c\uff0c\u539f\u88dd\u9032\u53e3\u57f9\u990a\u57fa\u4e0d\u6613\u53d7\u6f6e\uff0c\u4ea6\u6c92\u6709\u5206\u88dd\u9810\u6df7\u4e4b\u7591\u616e\u3002\u5176Harlequin\u2122\u5448\u8272\u57f9\u990a\u57fa\u8207\u5404\u5f0f\u57f9\u990a\u57fa\u6dfb\u52a0\u7269\uff0c\u63d0\u4f9b\u5feb\u901f\u548c\u6e96\u78ba\u7684\u65b9\u6cd5\u5206\u96e2\u8207\u5224\u8b80\u76ee\u6a19\u5fae\u751f\u7269\u3002\u96d9\u8272\u5224\u8b80\uff0c\u7c21\u6613\u5feb\u901f\uff1b\u5728\u98df\u54c1\u5b89\u5168\u53ca\u81e8\u5e8a\u8a3a\u65b7\u7686\u7b26\u5408\u76f8\u95dc\u898f\u7bc4\uff08ISO 9001:2000\u548cISO13485\uff0c\u4ee5\u53ca\u6b50\u6d32IVD\u6307\u4ee4CE\u6a19\u8a8c\uff09\u3002\u5206\u751f\u5c08\u7528\u57f9\u990a\u57faLB Broth\u8207LB Agar\uff0c\u65b9\u4fbf\u9032\u884cDNA Cloning\u3001Yeast\u57f9\u990a\u7b49\u5be6\u9a57\uff0c\u662f E. Coli \u8868\u73fe\u7cfb\u7d71\u6700\u4f73\u7684\u9078\u64c7\uff01<\/p>\n

Harmonised<\/strong>\u85e5\u5178\u7121\u83cc\u6e2c\u8a66\u4e4b\u57f9\u990a\u57fa<\/strong> |\u00a0 TSB <\/strong>\u7d30\u83cc\u548c\u771f\u83cc\u751f\u9577\u4e4b\u57f9\u990a\u57fa<\/strong>,<\/strong>\u8ca8\u865f<\/strong>LAB004-A<\/strong><\/a><\/p>\n

\u9069\u7528\u65bc\u7d30\u83cc\u548c\u771f\u83cc\u7684\u4e00\u7a2e\u901a\u7528\u578b\u7684\u71df\u990a\u57f9\u990a\u57fa\u3002\u70baHarmonised\u85e5\u5178\uff08USP\/ EP\/ JP\uff09\u4e2d\uff0c\u85e5\u7269\u7522\u54c1\u7684\u7121\u83cc\u6e2c\u8a66\u8981\u6c42\u4e4b\u57f9\u990a\u57fa\u3002\u4ea6\u5ee3\u6cdb\u7528\u65bc\u8840\u57f9\u990a\u57fa\uff0c\u65bc\u9ad8\u6c34\u6e96\u4e4b\u78b3\u6c34\u5316\u5408\u7269\u4e0b\u53ef\u8fc5\u901f\u589e\u9577\u548c\u96a8\u5f8c\u9178\u751f\u7522\u5f8c\u96a8\u5f8c\u6b7b\u4ea1\u3002The medium is widely used for blood cultures although the high carbohydrate level may cause rapid growth and subsequent death of acid-producing organisms<\/p>\n

Plate count agar<\/strong>\uff0c<\/strong>PCA<\/strong>\u57f9\u990a\u76bf\u8a08\u6578\u57f9\u990a\u57fa<\/strong>\\<\/strong>\u7e3d\u751f\u83cc\u6578\u57f9\u990a\u57fa<\/strong> | <\/strong>\u8ca8\u865f<\/strong>LAB149-A<\/strong><\/a><\/p>\n

\u570b\u969b\u6a19\u6e96PCA\uff1b\u7528\u65bc\u7d30\u83cc\u7e3d\u6578\u7684\u6e2c\u5b9a\uff08GB2008\u3001SN\u6a19\u6e96\uff09\u3002\u5546\u54c1\u5316\u7d30\u83cc\u7528\u57f9\u990a\u57faPCA\uff1a\u4e3b\u8981\u7528\u65bc\u7d30\u83cc\u57f9\u990a\uff1b\u6216\u4ea6\u53ef\u9078\u53d6\u80f0\u86cb\u767d\u5927\u8c46\u74ca\u8102\u57f9\u990a\u57fa\uff08Tryptone soy agar\uff0cTSA\uff09\u4e8c\u8005\u4e4b\u4e00\u57f9\u990a\u7d30\u83cc\u3002\u9ef4\u83cc\u53ca\u9175\u6bcd\u83cc\u57f9\u990a\uff0c\u4f7f\u7528\u6c99\u6c0f\u8461\u8404\u7cd6\u57f9\u990a\u57fa\uff08Sabouraud Dextrose Agar\uff0cSDA\uff09 \u6216\u99ac\u9234\u85af\u8461\u8404\u7cd6\u57f9\u990a\u57fa\uff08Potato dextrose agar\uff0cPDA\uff09\u3002Potato Dextrose Agar\u00a0 | \u99ac\u9234\u85af\u8461\u8404\u7cd6\u6d0b\u83dc\u57f9\u990a\u57fa |\u8ca8\u865fLAB098-A<\/p>\n

Aeromonas Agar <\/strong>\u6c23\u55ae\u80de\u83cc\u57f9\u990a\u57fa<\/strong> |<\/strong>\u8ca8\u865f<\/strong>LAB167<\/strong><\/a><\/p>\n

\u6c23\u55ae\u80de\u83cc\u57f9\u990a\u57fa\u70ba\u6c23\u55ae\u80de\u83cc\u5c6c\u9078\u64c7\u6027\u57f9\u990a\u57fa\u3002\u53ef\u5206\u96e2\u5f9e\u98df\u54c1\uff0c\u81e8\u5e8a\u548c\u74b0\u5883\u6a23\u54c1\u7684Aeromonas spp.\u3002Aeromonas Agar is a highly selective medium for the isolation of Aeromonas spp. from food, clinical and environmental samples.<\/p>\n

2xYT Agar\u00a0M13<\/strong>\u566c\u83cc\u9ad4\u751f\u9577\u4e4b\u57f9\u990a\u74ca\u8102\u51dd\u81a0<\/strong> |<\/strong>\u8ca8\u865f<\/strong>LAB180<\/strong><\/a><\/p>\n

2\u00d7YT\u57f9\u990a\u74ca\u8102\u51dd\u81a0\uff0c\u63d0\u4f9b\u7d72\u72c0\u55ae\u93c8\u566c\u83cc\u9ad4\u5bbf\u4e3b\u7d30\u80de\u7684\u751f\u9577\uff0c\u4f8b\u5982M13\u566c\u83cc\u9ad4\u3002An agar version of 2xYT broth, for the growth of host cells of filamentous single stranded bacteriophages e.g. the M13 phage.<\/p>\n

Acid Hydrolysed Casein<\/strong>\u71b1\u9178\u916a\u86cb\u767d\u53ef\u6eb6\u6027\u86cb\u767d\u6c34\u89e3\u7269<\/strong> |<\/strong>\u8ca8\u865f<\/strong>MC007<\/strong><\/a><\/p>\n

\u662f\u4e00\u7a2e\u53ef\u6eb6\u6027\u86cb\u767d\u6c34\u89e3\u7269\uff0c\u7531\u71b1\u9178\u916a\u86cb\u767d\u6d88\u5316\u800c\u4f86\u3002\u4e0d\u9808\u751f\u9577\u56e0\u6578\uff0c\u7dad\u751f\u7d20\u548c\u62ee\u6297\u5291\uff0c\u9069\u5408\u65bc\u86cb\u767d\u8cea\u4f86\u6e90\u4e4b\u6297\u751f\u7d20\u548c\u7dad\u751f\u7d20\u6e2c\u5b9a\u3002A soluble protein hydrolysate obtained by digesting casein with hot acid. It is almost free from growth factors, vitamins and antagonists, and these qualities make it suitable for use as protein source in media for antibiotic and vitamin assays.<\/p>\n

Agar No 1 Bacteriological |<\/strong>\u8ca8\u865f<\/strong>MC002<\/strong><\/a><\/p>\n

\u9ad8\u6e05\u6670\u4e4b\u74ca\u8102\u57f9\u990a\u57fa\uff0c\u5177\u6709\u826f\u597d\u51dd\u81a0\u6027\u80fd\u548c\u91d1\u5c6c\u96e2\u5b50\u6fc3\u5ea6\u4f4e\u7279\u6027\u3002\u9069\u7528\u65bc\u6240\u6709\u7684\u7d30\u83cc\uff0c\u5305\u62ec\u654f\u611f\u6027\u6e2c\u8a66\u548cpour plate\u4e4b\u6280\u8853\u3002A high clarity agar with good gelling properties and a low concentration of metal irons. The agar is suitable for all bacteriological purposes including sensitivity testing and pour plate techniques<\/p>\n

Yeast extract | <\/strong>\u9175\u6bcd\u8403\u53d6\u7269<\/strong>(yeast extract) |<\/strong>\u8ca8\u865f<\/strong>MC100-A<\/strong><\/a><\/p>\n

\u53ef\u4ee5\u63d0\u9ad8 E. Coli DH5\u03b1 \u5bc6\u5ea6\u9054\u50b3\u7d71 LB broth \u7684\u5169\u500d\u3002Yeast extract \u63d0\u4f9b\u4e86\u8c50\u5bcc\u7684\u71df\u990a\u6210\u5206\uff0cSaccharomyces\u9175\u6bcd\u7d30\u80de\u7d93\u904e\u81ea\u52d5\u5206\u89e3(autolysate)\u5f8c\uff0c\u9664\u4e86\u5bcc\u542b\u5404\u5f0f\u5fae\u751f\u7269\u4ee5\u53ca\u52d5\u7269\u7d30\u80de\u751f\u9577\u6240\u9700\u7684\u80fa\u57fa\u9178\u6c2e\u6e90\u5916\uff0c\u9084\u6709\u542b\u91cf\u6975\u9ad8\u7684\u53ef\u6eb6\u6027\u7dad\u751f\u7d20B\u7fa4\u3002\u5305\u62ec vitamins\u3001amino acids\u3001peptides \u8207 carbohydrates \u7b49\u3002\u5176\u9ad8\u71df\u990a\u50f9\u503c\u4e5f\u662f\u80ce\u725b\u8840\u6e05 FBS \u6700\u4f73\u66ff\u4ee3\u7269\uff0c\u662f\u751f\u7269\u88fd\u7a0b\u4e2d\u6975\u5177\u91cd\u8981\u6027\u7684\u975e\u52d5\u7269\u6027\u86cb\u767d\u4e4b\u4f86\u6e90\u3002\u56e0\u6b64\u57f9\u990a\u57fa\u914d\u65b9\u3001\u5fae\u751f\u7269\u767c\u9175\u539f\u6599\u3001\u8840\u6e05\u66ff\u4ee3\u7269\u3001\u6297\u9ad4\u751f\u7522\u8207\u86cb\u767d\u8cea\u85e5\u7269\u7b49\u90fd\u63a1\u7528yeast extract\u4f5c\u70ba\u71df\u990a\u4f86\u6e90\u3002<\/p>\n

Tryptone | <\/strong>\u916a\u86cb\u767d<\/strong>(casein)<\/strong>\u6c34\u89e3\u7269<\/strong> | <\/strong>\u8ca8\u865f<\/strong>MC005-A<\/strong><\/a><\/p>\n

Tryptone\u5e38\u898b\u65bc\u5fae\u751f\u7269\u57f9\u990a\u8207\u767c\u9175\u4f7f\u7528\uff0c\u4e5f\u662f\u7f8e\u570b\u85e5\u5178\u898f\u7bc4\u7684\u6a19\u6e96\u57f9\u990a\u57fa\u6210\u5206\u4e4b\u3127\u3002Tryptone\u662f\u916a\u86cb\u767d(casein)\u7d93\u7531\u80f0\u81df\u9175\u7d20\u6c34\u89e3(pancreatic digest)\u800c\u6210\u7684\u71df\u990a\u6210\u5206\uff0c\u4e5f\u662f\u4e00\u822c\u6240\u8b02\u80f0\u6c34\u89e3\u86cb\u767d\u7684\u901a\u7528\u540d\u7a31\u3002\u916a\u86cb\u767d\u662f\u725b\u5976\u4e2d\u4e3b\u8981\u7684\u86cb\u767d\u8cea\u6210\u5206\uff0c\u5bcc\u542b\u5404\u7a2e\u80fa\u57fa\u9178\u6c2e\u6e90\uff0c\u5c24\u5176\u662f\u8272\u80fa\u9178(tryptophan)\u6bd4\u4f8b\u76f8\u7576\u9ad8\u3002Tryptone\u7684\u7528\u9014\u5f88\u5ee3\uff0c\u662fLB medium\u3001Terrific broth\u3001Tryptic soy medium (TSB)\u8207Fluid Thioglycollate Medium (FTM)\u7b49\u5e38\u7528\u57f9\u990a\u57fa\u7684\u4e3b\u8981\u6210\u5206\uff0c\u4e5f\u662f\u6297\u751f\u7d20\u3001\u80de\u5916\u86cb\u767d\u8cea(extra-cellular protein)\u3001\u5e79\u64fe\u7d20(interferon)\u8207\u75ab\u82d7\u6bd2\u7d20\u7b49\u767c\u9175\u88fd\u7a0b\u4e2d\u91cd\u8981\u7684\u57f9\u990a\u539f\u6599\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u7522\u54c1\u7de8\u865f<\/strong><\/td>\n\u7522\u54c1\u540d\u7a31<\/strong><\/td>\n\u5305\u88dd<\/strong><\/td>\n<\/tr>\n
LAB004-A<\/td>\nTryptone Soy Broth USP (Soybean Casein Digest Medium)<\/td>\n500g<\/td>\n<\/tr>\n
LAB011-A<\/td>\nTryptone Soy Agar USP (Soybean Casein Digest Medium)<\/td>\n500g<\/td>\n<\/tr>\n
LAB028-A<\/td>\nBlood Agar Base<\/td>\n500g<\/td>\n<\/tr>\n
LAB061-A<\/td>\nEosin Methylene Blue Agar (Levine)<\/td>\n500g<\/td>\n<\/tr>\n
LAB093-A<\/td>\nMRS Agar<\/td>\n500g<\/td>\n<\/tr>\n
LAB094-A<\/td>\nMRS Broth<\/td>\n500g<\/td>\n<\/tr>\n
LAB098-A<\/td>\nPotato Dextrose Agar<\/td>\n500g<\/td>\n<\/tr>\n
LAB108-A<\/td>\nPseudomonas Agar Base<\/td>\n500g<\/td>\n<\/tr>\n
LAB149-A<\/td>\nPlate Count Agar<\/td>\n500g<\/td>\n<\/tr>\n
LAB168-A<\/td>\nL.B. Agar<\/td>\n500g<\/td>\n<\/tr>\n
LAB169-A<\/td>\nLB Broth<\/td>\n500g<\/td>\n<\/tr>\n
LAB196-A<\/td>\nLauryl Tryptose Broth<\/td>\n500g<\/td>\n<\/tr>\n
MC001-A<\/td>\nYeast Extract Powder<\/td>\n500g<\/td>\n<\/tr>\n
MC003-A<\/td>\nSoy Peptone<\/td>\n500g<\/td>\n<\/tr>\n
MC005-A<\/td>\nTryptone<\/td>\n500g<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u66f4\u591a\u76f8\u95dc\u5546\u54c1 \u5b89\u5168\u6838\u9178\u67d3\u5291\u5546\u54c1<\/a>| Agarose |\u5fae\u751f\u7269\u57f9\u990a\u57fa<\/a> | \u5fae\u751f\u7269\u6297\u751f\u7d20<\/a> | \u8cea\u9ad4DNA\u8403\u53d6\u5957\u7d44<\/a> |Mupid 2 DNA\u6c34\u6e96\u96fb\u6cf3\u69fd<\/a>| 3M\u5fae\u751f\u7269\u5feb\u901f\u6aa2\u6e2c\u7247<\/a><\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

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