{"id":16272,"date":"2018-03-29T10:53:28","date_gmt":"2018-03-29T02:53:28","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=16272"},"modified":"2022-07-20T16:56:16","modified_gmt":"2022-07-20T08:56:16","slug":"epcam-lentiviral-vector-human-cmv-%e6%85%a2%e7%97%85%e6%af%92%e8%bc%89%e9%ab%94-%e8%b2%a8%e8%99%9flv149409","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=16272","title":{"rendered":"EPCAM Lentiviral Vector (Human) (CMV) \u6162\u75c5\u6bd2\u8f09\u9ad4 | \u8ca8\u865fLV149409"},"content":{"rendered":"

EPCAM Lentiviral Vector (Human) (CMV) \u6162\u75c5\u6bd2\u8f09\u9ad4 | \u8ca8\u865fLV149409<\/a><\/span><\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>: \u00a0<\/strong>EPCAM Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV)<\/u>\u6162\u75c5\u6bd2\u8868\u73fe\u8f09\u9ad4\u3002EPCAM<\/u>\u57fa\u56e0 (Accession Number BC014785<\/u><\/a><\/u>) \u67b6\u69cb\u65bcpLenti-GIII-CMV<\/a> (Human)\u8868\u73fe\u8f09\u9ad4\uff0c\u7531CMV\u555f\u52d5\u5b50\u9a45\u52d5\uff0c\u6c92\u6709\u4efb\u4f55\u6a19\u7c64\u6216Reporter\u3002\u53ef\u76f4\u63a5\u4ee5\u975e\u75c5\u6bd2\u8cea\u7c92\u9032\u884c\u8f49\u67d3\u7528\u65bc\u5373\u6642\u8868\u9054\uff0c\u6216\u5305\u88dd\u6210\u6162\u75c5\u6bd2\u9846\u7c92\u7528\u65bc\u9ad8\u6548\u8f49\u5c0e\u548c\u7a69\u5b9a\u6574\u5408\u8868\u9054\u3002\u75c5\u6bd2\u5305\u88dd\u7cfb\u7d71\u5efa\u8b70\u4f7f\u7528ABMGOOD\u63d0\u4f9b\u4e4b\u8f49\u67d3\u8a66\u5291 Cat#\u00a0G074, Lentifectin\u2122<\/a> \u53ca\u512a\u5316\u4e4b\u75c5\u6bd2\u5305\u88dd\u7cfb\u5217LV003<\/a>\u3002\u6162\u75c5\u6bd2\u5305\u88dd\u7cfb\u5217\u7522\u54c1\u5305\u62ecLentivirus<\/u>\u6162\u75c5\u6bd2\u4f4e\u901f\u7d14\u5316<\/u>(\u7d14\u5316)\u00a0<\/a>\/\u00a0Lentivirus <\/u>\u6162\u75c5\u6bd2\u9ad8\u6fc3\u5ea6\u7d14\u5316<\/u>(\u7d14\u5316)\u00a0<\/a>\u4e26\u4ee5\u00a0Lentivirus<\/u>\u75c5\u6bd2<\/u>RT-QPCR<\/u>\u5b9a\u91cf<\/u>(\u5b9a\u91cf)<\/a>\u00a0\u7d14\u5316\u5f8c\u4e4b\u75c5\u6bd2\u529b\u50f9\u3002<\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Gene Insert<\/strong><\/td>\nEPCAM<\/td>\n<\/tr>\n
Accession Number<\/strong><\/td>\nBC014785<\/a>1,2,3,4,5<\/sup><\/td>\n<\/tr>\n
Vector<\/strong><\/td>\npLenti-GIII-CMV<\/a><\/td>\n<\/tr>\n
Vector Size<\/strong><\/td>\n8074bp<\/td>\n<\/tr>\n
Insert Size<\/strong><\/td>\n945 bp<\/td>\n<\/tr>\n
Species<\/strong><\/td>\nHuman<\/td>\n<\/tr>\n
Tags<\/strong><\/td>\nNo Tags<\/td>\n<\/tr>\n
Promoter<\/strong><\/td>\nCMV<\/td>\n<\/tr>\n
Selection Marker<\/strong><\/td>\nBacterial: Kanamycin. Mammalian: Puromycin<\/td>\n<\/tr>\n
Format<\/strong><\/td>\nLentiviral Plasmid<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u6162\u75c5\u6bd2\u6280\u8853\u4e4b\u9818\u5c0e\u8005abm\uff0c\u958b\u767c\u4e86\u4e00\u5957\u5168\u9762\u7684\u4eba\u985e\u3001\u5c0f\u9f20\u548c\u5927\u9f20\u57fa\u56e0\u6587\u5eab\uff0cClone\u5230\u6162\u75c5\u6bd2\u8f09\u9ad4\u6216\u88fd\u6210\u5373\u7528\u578b\u6162\u75c5\u6bd2\uff0c\u53ef\u7528\u65bc\u64cd\u7e31\u611f\u8208\u8da3\u57fa\u56e0\u65bc\u5bbf\u4e3b\u7d30\u80de\u8868\u9054\u3002\u70ba\u4e86\u4fc3\u9032\u6211\u5011\u7684\u6162\u75c5\u6bd2\u8f09\u9ad4\u7684\u61c9\u7528\u548c\u591a\u529f\u80fd\u6027\uff0cABM\u6162\u75c5\u6bd2\u8868\u9054\u7cfb\u7d71\u5177\u6709\u8a31\u591a\u7d44\u6210\u578b\u555f\u52d5\u5b50\uff0c\u6a19\u7c64\u5e8f\u5217\u548c\u5831\u5c0e\u57fa\u56e0\u4e4b\u7d44\u5408\u3002<\/p>\n

\"Neurons<\/p>\n

\u901a\u9053\u8996\u7d2b\u7d05\u8cea-2\u6162\u75c5\u6bd2C.reinhardtii\uff08ABM\uff0cLVP010100\uff09\u7684\u529f\u80fd\u8868\u9054\u3002 \u75c5\u6bd2\u4ee51MOI\u61c9\u7528\u65bc\u89e3\u96e2\u7684\u80da\u80ce\u5927\u9f20\u795e\u7d93\u5143\u57f9\u990a\u7269\u3002 A\uff0cC\uff0c\u986f\u793aEYFP\u8868\u9054\u7684\u5716\u50cf\uff0c\u7528\u767d\u8272\u7bad\u982d\u7a81\u51fa\u986f\u793a\u9078\u64c7\u7528\u65bc\u96fb\u751f\u7406\u5b78\u7684\u795e\u7d93\u5143\u3002 B\uff0cD\u5168\u7d30\u80de\u819c\u7247\u9257\u8a18\u9304\u986f\u793a\u4e86\u7531465nm\u5149\u6fc0\u767c\u7684\u793a\u4f8b\u5149\u96fb\u6d41\uff08\u4f86\u81eaA\uff0cC\uff09\u3002<\/p>\n

\"\"<\/p>\n

\u7528abm\u7684\u4ebaOVOL1\u6162\u75c5\u6bd2\u8f09\u9ad4\uff08Cat.No.LV250917\uff09\u8f49\u67d3\u5f8c\uff0c\u5c0d\u4ebaOVOL1\u9032\u884cqPCR\u64f4\u589e\u3002\u8207\u672a\u8f49\u67d3\u7684293T\u7d30\u80de\u76f8\u6bd4\uff0c\u5728\u7528abm\u7684OVOL1\u6162\u75c5\u6bd2\u8f09\u9ad4\uff08pLenti-GIII-CMV-C-term-HA\uff0cCat.No.LV250917\uff09\u8f49\u67d3\u7684293T\u7d30\u80de\u4e2d\u89c0\u5bdf\u5230\u7d04300\u500d\u7684\u904e\u5ea6\u8868\u9054\u3002 qPCR\u662f\u4f7f\u7528\u5ba2\u6236\u8a2d\u8a08\u7684\u5f15\u7269\u5b8c\u6210\u7684\u3002<\/p>\n\n\n\n\n\n\n\n
Promoter<\/th>\nApplications<\/th>\n<\/tr>\n
CMV<\/td>\nThe most widely used promoter. Very strong gene expression promoter in most cellular systems; weak activity in human and mouse stem cells.<\/td>\n<\/tr>\n
EF1\u03b1<\/td>\nStrong expression promoter; highly efficient in stem cells. Good for stable expression from long term cultures.<\/td>\n<\/tr>\n
UbC<\/td>\nRelatively low expression promoter. Sustains stable expression.<\/td>\n<\/tr>\n
PGK<\/td>\nMedium expression promoter. Highest efficiency in stem cells; Sustains stable activity in long term cultures of undifferentiated stem cells.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

EPCAM Lentiviral Vector (Human) (CMV) \u6162\u75c5\u6bd2\u8f09\u9ad4 | \u8ca8\u865fLV14940 […]<\/p>\n","protected":false},"author":3,"featured_media":16273,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[116],"tags":[2805],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/16272"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=16272"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/16272\/revisions"}],"predecessor-version":[{"id":16280,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/16272\/revisions\/16280"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/16273"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=16272"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=16272"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=16272"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}