{"id":16220,"date":"2018-03-26T16:56:06","date_gmt":"2018-03-26T08:56:06","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=16220"},"modified":"2025-05-02T10:11:03","modified_gmt":"2025-05-02T02:11:03","slug":"instantblue%e5%bf%ab%e9%80%9f%e5%9e%8b%e8%9b%8b%e7%99%bd%e8%b3%aa%e6%9f%93%e8%89%b2%e5%8a%91-%e8%b2%a8%e8%99%9fisb1l","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=16220","title":{"rendered":"InstantBlue\u5feb\u901f\u578b\u86cb\u767d\u8cea\u67d3\u8272\u5291 | Expedeon \u8ca8\u865fISB1L (Abcam ab119211)"},"content":{"rendered":"<p><strong><span style=\"color: #33cccc; font-size: 12pt;\"><a style=\"color: #33cccc;\" href=\"http:\/\/biopioneer.com.tw\/?p=16220\">InstantBlue\u5feb\u901f\u578b\u86cb\u767d\u8cea\u67d3\u8272\u5291 |\u8ca8\u865fISB1L<\/a>\u00a0\u00a0(Abcam ab119211)<\/span><\/strong><\/p>\n<p><strong>Western blot &#8211; InstantBlue<sup>\u00ae<\/sup>\u00a0Coomassie Protein Stain (ISB1L) (ab119211)<\/strong><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/www.abcam.com\/ps\/products\/119\/ab119211\/Images\/ab119211-383946-instantblue-coomassie-staining-protocol.jpg\" alt=\"\" \/><img decoding=\"async\" src=\"https:\/\/www.expedeon.com\/wp-content\/uploads\/2017\/12\/RunBlue-Coomassie-protein-stain.jpg\" alt=\"\" \/><\/p>\n<p>InstantBlue\u5feb\u901f\u578b\u86cb\u767d\u8cea\u67d3\u8272\u5291\uff0c\u7528\u65bc\u805a\u4e19\u70ef\u9170\u80fa\u51dd\u81a0\u3002\u5176\u7368\u7279\u7684\u4f5c\u7528\u6a5f\u5236\u572815\u5206\u9418\u5167\u5c0d\u86cb\u767d\u8cea\u9032\u884c\u67d3\u8272\uff0c\u540c\u6642\u7559\u4e0b\u6e05\u6670\u7684\u80cc\u666f\uff0c\u7121\u9700\u4fee\u5fa9\uff0c\u6e05\u6d17\u6216\u812b\u8272\u3002\u5176\u9748\u654f\u5ea6\u70ba5 ng\u6aa2\u6e2c\u5230\uff08BSA\uff09\u3002InstantBlue\u914d\u65b9\u5b89\u5168\u4f7f\u7528\uff0c\u6613\u65bc\u8655\u7f6e\u3002\u4e00\u4e9b\u9810\u88fd\u548c\u50b3\u7d71\u81ea\u88fd\u7684Coomassie \u00a0R-250\u67d3\u8272\u5291\u53ef\u80fd\u9700\u89813\u5c0f\u6642\u6216\u66f4\u9577\u6642\u9593\u624d\u80fd\u5b8c\u5168\u67d3\u8272\u51dd\u81a0\u3002InstantBlue\u53ef\u76f4\u63a5\u4f7f\u7528\uff0c\u4e26\u9810\u5148\u6df7\u54081\u5347\u5bb9\u91cf\u3002\u5176\u7368\u7279\u7684\u5c08\u5229\u6a5f\u5236\u958b\u59cb\u77ac\u9593\u67d3\u8272\uff0c\u4e26\u572815\u5206\u9418\u5167\u9054\u5230\u7d50\u679c\u3002InstantBlue\u7684\u4e0d\u542b\u4e59\u9178\u7684\u914d\u65b9\u5728\u67d3\u8272\u904e\u7a0b\u4e2d\u4e0d\u6703\u7532\u57fa\u5316\u6216\u4e59\u917c\u5316\u86cb\u767d\u8cea\uff0c\u4f7f\u5176\u8207\u8cea\u8b5c\u5100\u5177\u5b8c\u5168\u517c\u5bb9\u3002\u7531\u65bc\u5176\u7121\u7532\u9187\u6210\u5206\uff0cInstantBlue\u7121\u6bd2\u4e14\u53ef\u4e00\u6b21\u6027\u4f7f\u7528\u3002\u5176\u4ed6\u6c61\u6f2c\u9700\u8981\u6eb6\u5291\u8655\u7406\u7a0b\u5e8f\uff0c\u53ef\u80fd\u9700\u8981\u5fae\u6ce2\u8655\u7406\u624d\u80fd\u7522\u751f\u5371\u96aa\u7684\u7159\u9727\u3002 InstantBlue\u7684\u6709\u6548\u67d3\u8272\u548c\u5b89\u5168\u7d44\u5408\u610f\u5473\u8457\u4e0d\u9700\u8981\u5fae\u6ce2\uff0c\u6eb6\u5291\u8655\u7406\u6216\u901a\u98a8\u6ae5\u3002\u7532\u9187\u7684\u7f3a\u4e4f\u4e5f\u610f\u5473\u8457\u6c92\u6709\u51dd\u81a0\u6536\u7e2e\u3002\u6bcf\u500b\u8ff7\u4f60\u51dd\u81a0\u53ea\u970025ml\u7684\u7d93\u6fdf\u67d3\u8272\uff0c\u8b93\u60a8\u4f7f\u7528\u66f4\u5c11\u7684InstantBlue\u3002\u81ea\u88fd\u6eb6\u6db2\u548c\u4e00\u4e9b\u9810\u6df7\u5408\u6eb6\u6db2\u9700\u8981\u6bcf\u51dd\u81a0\u7d0450-100ml\u7684\u67d3\u8272\u5291\u3002 InstantBlue\u5c07\u6bcf\u74f6\u81f3\u5c11\u67d3\u827240\u500b\u5c0f\u578b\u51dd\u81a0\u3002InstantBlueTM is formulated ready to use. Gels can remain in stain for weeks without concern. Only proteins are stained resulting in extremely well defined blue bands on a highly transparent background. The reduction of background interference results in a better signal to noise ratio. Protein bands containing as low as 5 ng per band (BSA) can be detected.<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2025\/05\/3-InstantBlue%E5%BF%AB%E9%80%9F%E5%9E%8B%E8%9B%8B%E7%99%BD%E8%B3%AA%E6%9F%93%E8%89%B2%E5%8A%91-Expedeon-%E8%B2%A8%E8%99%9FISB1L-Abcam-ab119211.jpg\" \/><\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u7522\u54c1\u7279\u9ede\u8207\u512a\u52e2<\/strong><\/p>\n<ol>\n<li>\u8d85\u5feb\u67d3\u8272 &#8211; \u572815\u5206\u9418\u6216\u66f4\u77ed\u7684\u6642\u9593\u5167\u5b8c\u6210<\/li>\n<li>\u55ae\u6b65\u7a0b\u5e8f &#8211; \u7121\u9700\u6e05\u6d17\uff0c\u56fa\u5b9a\uff0c\u5fae\u6ce2\u6216\u812b\u8272<\/li>\n<li>\u9ad8\u9748\u654f\u5ea6 &#8211; \u53ef\u6aa2\u6e2c5ng\u983b\u6bb5*<\/li>\n<li>\u80cc\u666f\u6e05\u6670 &#8211; \u9ad8\u4fe1\u566a\u6bd4<\/li>\n<li>\u9748\u6d3b &#8211; \u4e0d\u6703\u904e\u5ea6\u8457\u8272<\/li>\n<li>\u5b9a\u91cf &#8211; \u6279\u91cf\u4e00\u81f4\u6027<\/li>\n<li>\u9ad8\u6548 &#8211; \u6bcf\u7a2e\u51dd\u81a0\u53ea\u970025ml<\/li>\n<li>\u5b89\u5168\u7684\u7d44\u6210 &#8211; \u7121\u6bd2\u3002 \u7121\u9700\u901a\u98a8\u6ae5\u6216\u6eb6\u5291\u8655\u7406<\/li>\n<li>\u7121\u7532\u9187 &#8211; \u7121\u51dd\u81a0\u6536\u7e2e\u6216\u86cb\u767d\u8cea\u7532\u57fa\u5316<\/li>\n<li>\u4e0d\u542b\u4e59\u9178 &#8211; \u4e0d\u542b\u86cb\u767d\u8cea\u4e59\u9170\u5316<\/li>\n<li>MS\u517c\u5bb91 &#8211; \u53ef\u6301\u7e8c\u767c\u5c55\u3002 \u6c92\u6709\u6b98\u7559\u7684\u7532\u57fa\u5316\u6216\u4e59\u91af\u5316<\/li>\n<\/ol>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2025\/05\/4-InstantBlue%E5%BF%AB%E9%80%9F%E5%9E%8B%E8%9B%8B%E7%99%BD%E8%B3%AA%E6%9F%93%E8%89%B2%E5%8A%91-Expedeon-%E8%B2%A8%E8%99%9FISB1L-Abcam-ab119211.jpg\" \/><\/p>\n<h3>InstantBlue\u2122\u00a0Coomassie Protein Stain Vs Competitors Coomassie Stains<\/h3>\n<table class=\"ib-table\">\n<thead>\n<tr class=\"ib-table__row\">\n<th class=\"ib-table__cell -head -first\"><\/th>\n<th class=\"ib-table__cell -head\">InstantBlue\u2122 (Expedeon)<\/th>\n<th class=\"ib-table__cell -head\">SimplyBlue\u2122 (Invitrogen)<\/th>\n<th class=\"ib-table__cell -head\">GelCode Blue\u2122 (Pierce)<\/th>\n<th class=\"ib-table__cell -head -last\">Home-made<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr class=\"ib-table__row -odd\">\n<td class=\"ib-table__cell -body -first\">No need to wash before staining<\/td>\n<td class=\"ib-table__cell -body\">\u2714\ufe0f<\/td>\n<td class=\"ib-table__cell -body\">\u274c<\/td>\n<td class=\"ib-table__cell -body\">\u274c<\/td>\n<td class=\"ib-table__cell -body -last\">\u274c<\/td>\n<\/tr>\n<tr class=\"ib-table__row -even\">\n<td class=\"ib-table__cell -body -first\">No need to fix before staining<\/td>\n<td class=\"ib-table__cell -body\">\u2714\ufe0f<\/td>\n<td class=\"ib-table__cell -body\">\u2714\ufe0f<\/td>\n<td class=\"ib-table__cell -body\">\u274c<\/td>\n<td class=\"ib-table__cell -body -last\">\u274c<\/td>\n<\/tr>\n<tr class=\"ib-table__row -odd\">\n<td class=\"ib-table__cell -body -first\">No need to heat or microwave<\/td>\n<td class=\"ib-table__cell -body\">\u2714\ufe0f<\/td>\n<td class=\"ib-table__cell -body\">\u274c<\/td>\n<td class=\"ib-table__cell -body\">\u2714\ufe0f<\/td>\n<td class=\"ib-table__cell -body -last\">\u2714\ufe0f<\/td>\n<\/tr>\n<tr class=\"ib-table__row -even\">\n<td class=\"ib-table__cell -body -first\">No need to destain<\/td>\n<td class=\"ib-table__cell -body\">\u2714\ufe0f<\/td>\n<td class=\"ib-table__cell -body\">\u274c<\/td>\n<td class=\"ib-table__cell -body\">\u274c<\/td>\n<td class=\"ib-table__cell -body -last\">\u274c<\/td>\n<\/tr>\n<tr class=\"ib-table__row -odd\">\n<td class=\"ib-table__cell -body -first\">No risk of over-staining if left too long<\/td>\n<td class=\"ib-table__cell -body\">\u2714\ufe0f<\/td>\n<td class=\"ib-table__cell -body\">\u274c<\/td>\n<td class=\"ib-table__cell -body\">\u274c<\/td>\n<td class=\"ib-table__cell -body -last\">\u274c<\/td>\n<\/tr>\n<tr class=\"ib-table__row -even\">\n<td class=\"ib-table__cell -body -first\">Non toxic and easily disposed of<\/td>\n<td class=\"ib-table__cell -body\">\u2714\ufe0f<\/td>\n<td class=\"ib-table__cell -body\">\u2714\ufe0f<\/td>\n<td class=\"ib-table__cell -body\">\u274c<\/td>\n<td class=\"ib-table__cell -body -last\">\u274c<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>InstantBlue\u5feb\u901f\u578b\u86cb\u767d\u8cea\u67d3\u8272\u5291 |\u8ca8\u865fISB1L\u00a0\u00a0(Abcam ab119211) Western [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":16221,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5261],"tags":[2810],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/16220"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=16220"}],"version-history":[{"count":5,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/16220\/revisions"}],"predecessor-version":[{"id":51113,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/16220\/revisions\/51113"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/16221"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=16220"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=16220"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=16220"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}