{"id":15915,"date":"2018-03-13T12:56:55","date_gmt":"2018-03-13T04:56:55","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=15915"},"modified":"2022-07-20T17:17:40","modified_gmt":"2022-07-20T09:17:40","slug":"mouse-anti-chicken-cd3-unlb-chickenpigeon-cd3%e8%b2%a8%e8%99%9f8200-01","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=15915","title":{"rendered":"Mouse Anti-Chicken CD3-UNLB | Chicken\/Pigeon CD3\u8ca8\u865f8200-01"},"content":{"rendered":"

Mouse Anti-Chicken CD3-UNLB<\/strong> | <\/strong>\u8ca8\u865f<\/strong>82<\/strong>00-0<\/strong>1\u00a0<\/strong>\u898f\u683c<\/strong>1ml<\/strong> (0.5 mg\/mL)<\/strong><\/a><\/span><\/p>\n

Chicken\/Pigeon CD3<\/strong><\/p>\n

\u7d30\u80de\u514d\u75ab\u5c08\u5bb6<\/strong>\u6975\u529b\u63a8\u85a6<\/strong>SouthernBiotech<\/strong>\u9ad8\u54c1\u8cea\u4e8c\u7d1a\u6297\u9ad4<\/strong><\/p>\n

\u7f8e\u570b\u6297\u9ad4\u751f\u7522\u5546Southern Biotech\u65bc1982\u5e74\uff0c\u7531UAB\u7d30\u80de\u514d\u75ab\u5c08\u5bb6Dr. Max Cooper\u8207\u5be6\u9a57\u5ba4\u6210\u54e1\u5171\u540c\u5275\u7acb\u3002SouthernBiotech\u81f4\u529b\u65bc\u751f\u7522\u9ad8\u54c1\u8cea\u4e8c\u7d1a\u6297\u9ad4\u3002\u7269\u7a2e\u7a2e\u985e\u9f4a\u5168\uff0c\u5305\u62ec\u6709Bovine\u3001Canine\u3001Chicken\u3001Cynomolgus\u3001Donkey\u3001Equine\u3001Feline\u3001Goat\u3001Guinea Pig\u3001Hamster\u3001Human\u3001Monkey\u3001Mouse\u3001Porcine\u3001Rabbit\u3001Rat\u3001Rhesus\u3001Sheep\u3001Turkey\u3002\u5177\u6709\u87a2\u5149\u7269\u8cea\uff08FITC\u3001PE\u3001APC\u3001SPRD\u3001PE\/CY5.5\u3001APC\/CY5.5\u3001Spectral Red\u3001Alexa 488\u3001Alexa 647\u3001Pacific Blue\uff09\u5404\u7a2e\u87a2\u5149\u6a19\u8a8c\u3001\u53e6\u5177\u9175\u7d20\uff08HRP\u3001AP\uff09\u3001\u6216\u672a\u6a19\u8a8c\u53caLow Endotoxin\/Azide Free\uff08LE\/AF\uff09\u7b49\u6a19\u5b9a\u3002<\/p>\n

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Paraffin embedded chicken spleen tissue was stained with Mouse Anti-Chicken CD3-UNLB (SB Cat. No. 8200-01; right) followed by Anti-Mouse IgG-HRP and DAB.<\/p>\n

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Frozen vIBDV infected bursal tissue section was stained with Mouse Anti-Chicken CD3-UNLB (SB Cat. No. 8200-01) followed by a secondary reagents and DAB. Image from Rauf A, Khatri M, Murgia MV, Jung K, Saif YM. Differential modulation of cytokine, chemokine and Toll like receptor expression in chickens infected with classical and variant infectious bursal disease virus. Vet Res. 2011;42:85. Figure 3[A(e)]<\/p>\n

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Frozen chicken ovary section was stained with Mouse Anti-Chicken CD3-UNLB (SB Cat. No. 8200-01) followed by an HRP conjugated secondary antibody, DAB, and hematoxylin. Image from Bradaric MJ, Barua A, Penumatsa K, Yi Y, Edassery SL, Sharma S, et al. Sphingosine-1 phosphate receptor (S1p1), a critical receptor controlling human lymphocyte trafficking, is expressed in hen and human ovaries and ovarian tumors. J Ovarian Res. 2011;4:4. Figure 6(b)<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Clone<\/strong><\/td>\nCT-3<\/td>\n<\/tr>\n
Isotype<\/strong><\/td>\nMouse (BALB\/c) IgG1<\/sub>\u03ba<\/td>\n<\/tr>\n
Isotype Control<\/strong><\/td>\nMouse IgG1<\/sub>-UNLB<\/a><\/td>\n<\/tr>\n
Immunogen<\/strong><\/td>\nChicken thymocytes and Ig–<\/sup>\u00a0blood mononuclear cells<\/td>\n<\/tr>\n
Specificity<\/strong><\/td>\nChicken\/Pigeon CD3<\/td>\n<\/tr>\n
Alternalte Name(s)<\/strong><\/td>\nT3\/TCR complex<\/td>\n<\/tr>\n
Description<\/strong><\/td>\nCD3 is a member of the T cell receptor-associated CD3 complex. The monoclonal antibody CT-3 recognizes a complex of at least three polypeptides of Mr 20, 19, and 17 kDa (two of which are N-glycosylated) on chicken T cells. The antibody also coprecipitates a polypeptide of 90 kDa from digitonin solubilized T cell lysates, which can be reduced to two polypeptides of Mr 50 and 40 kDa.<\/td>\n<\/tr>\n
Format\/Conjugate<\/strong><\/td>\nUNLB (Unconjugated)<\/td>\n<\/tr>\n
Buffer Formulation<\/strong><\/td>\nSupplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added<\/td>\n<\/tr>\n
Concentration<\/strong><\/td>\n0.5 mg\/mL<\/td>\n<\/tr>\n
Volume<\/strong><\/td>\n1.0 mL<\/td>\n<\/tr>\n
Storage & Handling<\/strong><\/td>\nStore at 2-8\u00b0C
\nPlease refer to product specific\u00a0SDS<\/a><\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n\n

Applications for relevant formats of this clone include –<\/p>\n

Flow Cytometry \u2013 Quality tested\u00a01,5,10-18<\/sup>
\nImmunohistochemistry-Frozen Sections \u2013 Reported in literature\u00a02-8<\/sup>
\nImmunohistochemistry-Paraffin Sections \u2013 Reported in literature\u00a09<\/sup>
\nImmunoprecipitation \u2013 Reported in literature\u00a01<\/sup>
\nStimulation \u2013 Reported in literature\u00a01<\/sup><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n


\nIsotype control <\/strong>\u5206\u578b\u5c0d\u7167\u7d44<\/strong><\/a> | <\/strong>\u5206\u578b\u5c0d\u7167\u7d44\u5957\u7d44<\/strong><\/a> | <\/strong>\u6297\u9ad4\u5206\u578b<\/strong>ELISA <\/strong>\u5957\u7d44<\/strong><\/a> | <\/strong>anti-rabbit <\/strong>\u4e8c\u7d1a\u6297\u9ad4<\/strong><\/a> | <\/strong>anti-mouse<\/strong>\u4e8c\u7d1a\u6297\u9ad4<\/strong><\/a> | <\/strong><\/p>\n

References<\/strong><\/p>\n

    \n
  1. Chen CH, Ager LL, Gartland GL, Cooper MD. Identification of a T3\/T cell receptor complex in chickens. J Exp Med. 1986;164:375-80. (Immunogen, FC, IP, Stim)<\/li>\n
  2. Njenga MK, Dangler CA. Endothelial MHC class II antigen expression and endarteritis associated with Marek’s disease virus infection in chickens. Vet Pathol. 1995;32:403-11. (IHC-FS)<\/li>\n
  3. Tanimura N, Sharma JM. Appearance of T cells in the bursa of Fabricius and cecal tonsils during the acute phase of infectious bursal disease virus infection in chickens. Avian Dis. 1997;41:638-45. (IHC-FS)<\/li>\n
  4. Zheng W, Yoshimura Y. Organ-specificity of estrogen effects on the induction of immunocompetent cells in the chicken. J Poult Sci. 2001;38:41-9. (IHC-FS)<\/li>\n
  5. Sheela RR, Babu U, Mu J, Elankumaran S, Bautista DA, Raybourne RB, et al. Immune responses against Salmonella enterica serovar enteritidis infection in virally immunosuppressed chickens. Clin Diagn Lab Immunol. 2003;10:670-9. (FC, IHC-FS)<\/li>\n
  6. Pantin-Jackwood MJ, Brown TP, Huff GR. Proventriculitis in broiler chickens: immunohistochemical characterization of the lymphocytes infiltrating the proventricular glands. Vet Pathol. 2004;41:641-8. (IHC-FS)<\/li>\n
  7. Hansell C, Zhu XW, Brooks H, Sheppard M, Withanage S, Maskell D, et al. Unique features and distribution of the chicken CD83+ cell. J Immunol. 2007;179:5117-25. (IHC-FS)<\/li>\n
  8. Schusser B, Collarini EJ, Yi H, Izquierdo SM, Fesler J, Pedersen D, et al. Immunoglobulin knockout chickens via efficient homologous recombination in primordial germ cells. Proc Natl Acad Sci USA. 2013;110:20170-5. (IHC-FS)<\/li>\n
  9. Solcan C, Solcan G, Cotea C. Immunotoxic action of ochratoxine A on lymphocytes from lymphoid tissues associated to gut mucosa in chickens. Bulletin UASVM Agriculture. 2010;67:283-90. (IHC-PS)<\/li>\n
  10. Koskinen R, G\u00f6bel TW, Tregaskes CA, Young JR, Vainio O. The structure of avian CD5 implies a conserved function. J Immunol. 1998;160:4943-50. (FC)<\/li>\n
  11. Conrad ML, Davis WC, Koop BF. TCR and CD3 antibody cross-reactivity in 44 species. Cytometry. 2007;71A:925-33. (FC)<\/li>\n
  12. Janardhana V, Broadway MM, Bruce MP, Lowenthal JW, Geier MS, Hughes RJ, et al. Prebiotics modulate immune responses in the gut-associated lymphoid tissue of chickens. J Nutr. 2009;139:1404-9. (FC)<\/li>\n
  13. Xue M, Shi X, Zhao Y, Cui H, Hu S, Cui X, et al. Effects of reticuloendotheliosis virus infection on cytokine production in SPF chickens. PLoS One. 2013;8(12):e83918. (FC)<\/li>\n
  14. Peng X, Zhang K, Bai S, Ding X, Zeng Q, Yang J, et al. Histological lesions, cell cycle arrest, apoptosis and T cell subsets changes of spleen in chicken fed aflatoxin-contaminated corn. Int J Environ Res Public Health. 2014;11:8567-80. (FC)<\/li>\n
  15. Vu Manh T, Marty H, Sibille P, Le Vern Y, Kaspers B, Dalod M, et al. Existence of conventional dendritic cells in Gallus gallus revealed by comparative gene expression profiling. J Immunol. 2014;192:4510-7. (FC)<\/li>\n
  16. Pleidrup J, Dalgaard TS, Norup LR, Permin A, Schou TW, Skovgaard K, et al. Ascaridia galli infection influences the development of both humoral and cell-mediated immunity after Newcastle Disease vaccination in chickens. Vaccine. 2014;32:383-92. (FC)<\/li>\n
  17. Laniewski P, Kuczkowski M, Chrz\u0105stek K, Wo\u017aniak A, Wyszy\u0144ska A, Wieliczko A, et al. Evaluation of the immunogenicity of Campylobacter jejuni CjaA protein delivered by Salmonella enterica sv. Typhimurium strain with regulated delayed attenuation in chickens. World J Microbiol Biotechnol. 2014;30:281-92. (FC)<\/li>\n
  18. Dudek K, Bednarek D. Cellular immune response of pigeons in the conditions of endotoxin fever and pyrogenic tolerance. Pol J Vet Sci. 2011;14:127-33. (FC, Pigeon Reactivity)<\/li>\n<\/ol>\n

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    Mouse Anti-Chicken CD3-UNLB | \u8ca8\u865f8200-01\u00a0\u898f\u683c1ml (0.5 mg\/m […]<\/p>\n","protected":false},"author":3,"featured_media":15916,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[136],"tags":[2857],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15915"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=15915"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15915\/revisions"}],"predecessor-version":[{"id":18083,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15915\/revisions\/18083"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/15916"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=15915"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=15915"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=15915"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}