{"id":15784,"date":"2018-03-05T14:13:57","date_gmt":"2018-03-05T06:13:57","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=15784"},"modified":"2022-07-20T17:40:03","modified_gmt":"2022-07-20T09:40:03","slug":"%e7%b4%b0%e8%83%9e%e8%89%b2%e7%b4%a0p450%e9%82%84%e5%8e%9f%e9%85%b6%ef%bc%88cpr%ef%bc%89%e6%b4%bb%e6%80%a7%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fk700-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=15784","title":{"rendered":"\u7d30\u80de\u8272\u7d20P450\u9084\u539f\u9176\uff08CPR\uff09\u6d3b\u6027\u8a66\u5291\u76d2 | \u8ca8\u865fK700-100"},"content":{"rendered":"

\u7d30\u80de\u8272\u7d20P450\u9084\u539f\u9176\uff08CPR\uff09\u6d3b\u6027\u8a66\u5291\u76d2 <\/strong>| <\/strong>\u8ca8\u865f<\/strong>K700-100<\/a><\/span><\/p>\n

Cytochrome P450 Reductase (CPR, EC 1.6.2.4) Activity Kit (Colorimetric)<\/em><\/strong><\/p>\n

\"\"<\/p>\n

\u7522\u54c1<\/strong>\u63cf\u8ff0\uff1a<\/strong>NADPH-\u7d30\u80de\u8272\u7d20P450\u9084\u539f\u9176\uff08CPR\uff0cEC1.6.2.4\uff09\u662f\u301c78kDa\u819c\u7d50\u5408\u7684\u9ec3\u7d20\u9176\uff0c\u5176\u50ac\u5316NADPH\u4e2d\u7684\u96fb\u5b50\u5411\u5167\u8cea\u7db2\u4e2d\u7d30\u80de\u8272\u7d20P450\u55ae\u6c27\u5316\u9176\uff08CYP\uff09\u5bb6\u65cf\u6210\u54e1\u4e4b\u8f49\u79fb\u3002 CPR\u542b\u6709\u5169\u500b\u7dca\u5bc6\u7d50\u5408\u7684\u9ec3\u7d20\u8f14\u56e0\u5b50FAD\u548cFMN\uff0c\u5b83\u5011\u53c3\u8207\u5f9eNADPH\u2192FAD\u2192FMN\u2192CYP\u7684\u96fb\u5b50\u9806\u5e8f\u8f49\u79fb\uff0c\u5c07NADPH\u6c27\u5316\u6210NADP +\u4e26\u5c07CYP\u8840\u7d05\u7d20\u90e8\u5206\u9084\u539f\u6210\u8207\u5e95\u7269\u548c\u6c27\u7d50\u5408\u7684\u4e9e\u9435\u72c0\u614b\u3002\u7531\u65bcCPR\u662f\u6240\u6709CYP\u540c\u529f\u9176\u7684\u529f\u80fd\u6240\u5fc5\u9700\u7684\uff0c\u5b83\u9664\u4e86\u5728\u67d0\u4e9b\u7dad\u751f\u7d20\u548c\u985e\u56fa\u9187\u6fc0\u7d20\u7684\u751f\u7269\u5408\u6210\u4e2d\u7684\u4f5c\u7528\u5916\uff0c\u9084\u5728\u85e5\u7269\u4e4b\u3001\u6709\u6a5f\u6c61\u67d3\u7269\u548c\u5176\u4ed6\u7570\u751f\u5316\u5408\u7269\u7684\u4ee3\u8b1d\u4e2d\u8d77\u95dc\u9375\u4f5c\u7528\u3002 BioVision\u7684\u7d30\u80de\u8272\u7d20P450\u9084\u539f\u9176\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2\uff0c\u5176\u8a2d\u8a08\u539f\u7406\u901a\u904eCPR\u5c07NADPH\u6c27\u5316\u6210\u5e7e\u4e4e\u7121\u8272\u7684\u63a2\u91dd\uff0c\u5c07\u5176\u8f49\u8b8a\u6210\u8272\u6fa4\u9bae\u8c54\u7684\u7522\u54c1\uff0c\u5176\u5438\u5149\u5ea6\u5cf0\u503c\u70ba460 nm\uff0c\u984f\u8272\u7522\u751f\u7387\u8207CPR\u6d3b\u6027\u6210\u6b63\u6bd4\u3002 CPR\u4f7f\u7528\u7684NADPH\u7531\u03b2-NADP +\u901a\u904e\u8461\u8404\u7cd6-6-\u78f7\u9178\u9176\u812b\u6c2b\u9176\uff08G6PDH\uff09\u901a\u904e\u8461\u8404\u7cd6-6-\u78f7\u9178\uff08G6P\uff09\u6c27\u5316\u62106-\u78f7\u9178-D-\u8461\u7cd6\u9178-1,5-\u5167\u916f\u539f\u4f4d\u7522\u751f\u3002\u8a72\u8a66\u5291\u76d2\u53ef\u7528\u65bc\u6e2c\u5b9a\u5404\u7a2e\u6a23\u54c1\u4e2d\u7684CPR\u6d3b\u6027\uff0c\u6aa2\u6e2c\u9650\u70ba\u6bcf\u6b21\u53cd\u61c9\u7d040.2 mU\u7684CPR\u6d3b\u6027\u3002\u70ba\u4e86\u8a55\u4f30\u53ef\u80fd\u5177\u6709\u80fd\u5920\u9084\u539f\u5e95\u7269\u7684\u5916\u4f86\u9084\u539f\u9176\u7684\u7c97\u751f\u7269\u6a23\u54c1\u4e2d\u7684CPR\u6d3b\u6027\uff0c\u5305\u62ecNADPH\u4f9d\u8cf4\u6027\u9ec3\u7d20\u86cb\u767d\u6291\u5236\u5291\u3002\u5728\u9019\u7a2e\u60c5\u6cc1\u4e0b\uff0c\u7279\u5b9a\u7684CPR\u6d3b\u6027\u53ef\u4ee5\u901a\u904e\u5728\u5b58\u5728\u548c\u4e0d\u5b58\u5728\u6291\u5236\u5291\u7684\u60c5\u6cc1\u4e0b\uff0c\u9032\u884c\u5e73\u884c\u53cd\u61c9\u4e26\u6e1b\u53bb\u7528\u5b58\u5728\u7684\u6291\u5236\u5291\u6aa2\u6e2c\u5230\u7684\u4efb\u4f55\u6b98\u7559\u6d3b\u6027\u4f86\u8a08\u7b97\u3002\u8a72\u8a66\u5291\u76d2\u5305\u542b\u8db3\u5920\u7684\u8a66\u5291\uff0c\u7528\u65bc\u4ee596\u5b54\u677f\u7684\u5f62\u5f0f\u9032\u884c100\u6b21\u53cd\u61c9\u3002<\/p>\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK700-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (460 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nEukaryotes<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nThe kit can detect CPR activity in a variety of sample types with a detection limit of ~0.2 mU per well.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Only 96-well plate format CPR assay kit on the market
\n\u2022 Only kit to use a synthetic colorimetric probe and a NADPH generating system
\n\u2022 Rapid, convenient and sensitive
\n\u2022 Kit contains all necessary reagents for accurate measurement of CPR activity down to 0.2 mU per well in a wide variety of sample types<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 CPR Assay Buffer
\n\u2022 G6P Standard
\n\u2022 Inhibitor (Diphenyleneiodonium Chloride, 10 mM)
\n\u2022 NADPH Substrate Mix
\n\u2022 G6P Standard Developer
\n\u2022 Human CPR Positive Control<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\u00a0<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

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