{"id":15582,"date":"2018-02-07T12:14:01","date_gmt":"2018-02-07T04:14:01","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=15582"},"modified":"2022-10-30T20:11:49","modified_gmt":"2022-10-30T12:11:49","slug":"brdu%e7%b4%b0%e8%83%9e%e5%a2%9e%e6%ae%96%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92-brdu-cell-proliferation-assay-kit%e8%b2%a8%e8%99%9fk306-1000","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=15582","title":{"rendered":"BrdU\u7d30\u80de\u589e\u6b96\u6e2c\u5b9a\u8a66\u5291\u76d2 | BrdU Cell Proliferation Assay Kit\u8ca8\u865fK306-1000 (abcam ab287841)"},"content":{"rendered":"

BrdU<\/strong>\u7d30\u80de\u589e\u6b96\u6e2c\u5b9a\u8a66\u5291\u76d2 <\/strong>| <\/strong>\u8ca8\u865f<\/strong>K306-1000<\/a>\u00a0(abcam ab287841)<\/span><\/p>\n

BrdU Cell Proliferation Assay Kit<\/em><\/strong><\/p>\n

\"\u539f\u7406\u8207\u77e5\u8b58\"<\/p>\n

5-\u6eb4-2-\u812b\u6c27\u5c3f\u82f7\uff085-bromo-2-deoxyuridine, BrdU\uff09\u662f\u5627\u5576\u985e\u4f3c\u7269 (\u00a0pyrimidine analog)\u3002 \u5b83\u88ab\u00a0incorporated\u00a0\u5230\u65b0\u5408\u6210\u7684\u589e\u6b96\u7d30\u80deDNA (proliferating cells) \u4e2d\uff0c\u4ee3\u66ff\u80f8\u82f7 (thymidine)\u3002 Biovision\u7684BrdU\u7d30\u80de\u589e\u6b96\u6e2c\u5b9a\u8a66\u5291\u76d2(BrdU Cell Proliferation Assay Kit, k306) \u4f7f\u7528\u5c0f\u9f20\u6297BrdU\u6297\u9ad4 (anti-BrdU antibody) \u6aa2\u6e2c\u647b\u5165\u7684BrdU\u3002 \u4f7f\u7528\u6297\u5c0f\u9f20HRP\u9023\u63a5\u7684\u4e8c\u6297 (\u00a0anti-mouse HRP-linked secondary antibody) \u4f86\u6aa2\u6e2c\u8207BrdU\u7d50\u5408\u7684\u6297BrdU\u6297\u9ad4\uff0c\u7136\u5f8c\u52a0\u5165TMB\uff08HRP substrate\uff09\u3002 \u986f\u8272\u7a0b\u5ea6\u8207\u647b\u5165\u7d30\u80de\u4e2d\u7684BrdU\u91cf\u6210\u6b63\u6bd4\uff0c\u53ef\u4ee5\u76f4\u63a5\u7528\u4f5c\u7d30\u80de\u589e\u6b96 (cell proliferation) \u7684\u6307\u6a19\u3002 \u8207\u5176\u4ed6\u7d30\u80de\u589e\u6b96\u6e2c\u5b9a\u76f8\u6bd4\uff0c\u8a72\u8a66\u5291\u76d2\u50c5\u6aa2\u6e2c\u589e\u6b96\u7d30\u80de\u800c\u4e0d\u6aa2\u6e2cseeded cells\u3002 \u9019\u7a2e\u9ad8\u5ea6\u654f\u611f\u7684\u975e\u653e\u5c04\u6027\u8a66\u5291\u76d2\u53ef\u6aa2\u6e2c\u5230\u5c11\u65bc50-100\u500b\u589e\u6b96\u7d30\u80de (proliferating cells)\u3002<\/p>\n

 <\/p>\n

\"\"<\/p>\n

\"k306\"<\/a><\/p>\n

\"\u540c\u8cea\u5546\u54c17\"<\/a><\/p>\n\n\n\n\n\n\n\n
Detection Method<\/strong><\/td>\nAbsorbance (450 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nN\/A<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nDetection and quantification of cell proliferation induced by growth factors, cytokines, mitogens, and nutrients.
\nAnalysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals.
\nDetermination of the inhibitory or stimulatory effects of various compounds on cell proliferation.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Convenient & non-radioactive
\n\u2022 High-throughput suitable
\n\u2022 Highly-sensitive: detect as low as 50-100 proliferating cells.
\n\u2022 Suitable for cell proliferation, chemotaxis and cytotoxicity<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 BrdU (1000X)
\n\u2022 Fixing\/Denaturing Solution
\n\u2022 BrdU Detection Antibody (300X)
\n\u2022 Anti-mouse HRP-linked Antibody (2000X)
\n\u2022 Antibody Diluent
\n\u2022 Wash Buffer (10X)
\n\u2022 TMB Substrate
\n\u2022 Stop Solution<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u5f15\u7528\u6587\u737b\"<\/p>\n

Ye Chen, Bromodomain and extraterminal proteins foster the core transcriptional regulatory programs and confer vulnerability in liposarcoma. Nat Commun., March 2019;\u00a0 30903020.<\/p>\n

Severa Bunda, CIC protein instability contributes to tumorigenesis in glioblastoma. Nat Commun., Feb 2019; 30737375.<\/p>\n

Chen, Si-Le et al. (2016) Targeting CRMP\u20114 by lentivirus\u2011mediated RNA interference inhibits SW480 cell proliferation and colorectal cancer growth, Exp Ther Med. 2016 Oct;12(4):2003-2008.<\/p>\n

\"smart\"<\/a>\"Spectrumlab<\/a><\/p>\n

\"\u5be6\u9a57\u8a2d\u50992\"<\/a>\"\u00e7\u008e\u00bb\u00e7\u0089\u0087\u00e6\u008e\u0083\u00e6\u008f\u008f\"<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

BrdU\u7d30\u80de\u589e\u6b96\u6e2c\u5b9a\u8a66\u5291\u76d2 | \u8ca8\u865fK306-1000\u00a0(abcam ab287841) BrdU Cell […]<\/p>\n","protected":false},"author":3,"featured_media":28510,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[2918],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15582"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=15582"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15582\/revisions"}],"predecessor-version":[{"id":35692,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15582\/revisions\/35692"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/28510"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=15582"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=15582"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=15582"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}