{"id":15520,"date":"2018-02-05T17:15:09","date_gmt":"2018-02-05T09:15:09","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=15520"},"modified":"2023-03-10T11:23:58","modified_gmt":"2023-03-10T03:23:58","slug":"bcaa%e6%94%af%e9%8f%88%e6%b0%a8%e5%9f%ba%e9%85%b8%ef%bc%88leu-ile-val%ef%bc%89%e6%af%94%e8%89%b2%e5%88%86%e6%9e%90-branched-chain-amino-acid-colorimetric-assay-kit%e8%b2%a8%e8%99%9fk564-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=15520","title":{"rendered":"BCAA\u652f\u93c8\u6c28\u57fa\u9178\uff08Leu \/ Ile \/ Val\uff09\u6bd4\u8272\u5206\u6790| Branched Chain Amino Acid Colorimetric Assay Kit\u8ca8\u865fK564-100 (ABCAM ab83374)"},"content":{"rendered":"

\u652f\u93c8\u6c28\u57fa\u9178\uff08Leu \/ Ile \/ Val\uff09\u6bd4\u8272\u5206\u6790\u8a66\u5291\u76d2<\/strong>| <\/strong>\u8ca8\u865f<\/strong>K564-100<\/a>\u00a0abcam\u8ca8\u865fab83374\u00a0(ABCAM ab83374)<\/span><\/p>\n

Branched Chain Amino Acid (Leu\/Ile\/Val) Colorimetric Assay Kit<\/span><\/p>\n

\"biovision\u00e5\u0095\u0086\u00e5\u0093\u0081\u00e6\u008f\u0092\u00e5\u009c\u0096\"<\/p>\n

\"\u00e5\u008e\u009f\u00e7\u0090\u0086\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\"BCAA\u652f\u93c8\u6c28\u57fa\u9178 (branched-chain amino acids, BCAA) \u6216BCAA\u2019s\uff0c\u662f\u6307\u5177\u6709\u975e\u7dda\u6027\u8102\u80aa\u65cf\u5074\u93c8\u7684\u6c28\u57fa\u9178 (non-linear aliphatic side-chains) \uff0c\u5373\u4eae\u6c28\u9178\u00a0(leucine)\uff0c\u7570\u4eae\u6c28\u9178(isoleucine)\u548c\u7e88\u6c28\u9178(valine)\u3002BCAA\u4e2d\u6587\u70ba\u652f\u93c8\u80fa\u57fa\u9178\uff08Branched-Chain Amino Acid\uff09\u662f\u4eba\u9ad4\u4e2d3\u7a2e\u91cd\u8981\u7684\u5fc5\u9808\u80fa\u57fa\u9178 (essential amino acids)\uff0c\u5305\u62ec\u7e88\u6c28\u9178(valine)\u3001\u4eae\u6c28\u9178( leucine)\u53ca\u7570\u4eae\u6c28\u9178(isoleucine)\uff08\u7279\u5fb5\u70ba\u5074\u93c8\u6709\u5206\u652f\u7d50\u69cb\uff09\u9019\u4e09\u7a2e\u5fc5\u9700\u6c28\u57fa\u9178\u4eba\u9ad4\u9aa8\u9abc\u808c(skeletal muscle)\u76841\/3\u5de6\u53f3\u7d44\u6210\u00a0\u3002\u76ee\u524dBCAA\u652f\u93c8\u6c28\u57fa\u9178\u88ab\u7528\u65bc\u81e8\u5e8a\u4e0a\u5e6b\u52a9\u71d2\u50b7\u53d7\u5bb3\u8005(burn victims)\u7684\u5eb7\u5fa9(recovery)\uff0c\u4ee5\u53ca\u70ba\u904b\u52d5\u54e1 (athletes) \u88dc\u5145\u904b\u52d5\u6240\u9700\u529b\u91cf\u3002\u00a0BCAA\u652f\u93c8\u6c28\u57fa\u9178\uff0c\u4e3b\u8981\u662fLeu\uff0c\u53ef\u4ee5\u523a\u6fc0\u80f0\u5cf6\u7d20\u5206\u6ccc(insulin)\u3002\u652f\u93c8\u6c28\u57fa\u9178\u4e5f\u727d\u6d89(\u00a0implicated) \u5230\u5176\u4ed6\u5404\u7a2e\u751f\u7406\u6548\u61c9(\u00a0physiological effects)\u3002 BioVision\u7684BCAA\u5206\u6790\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u55ae\u65b9\u4fbf\u7684\u65b9\u6cd5\uff0c\u4f86\u6e2c\u91cf\u5404\u7a2e\u751f\u7269\u6a23\u54c1\u4e2d\u7684BCAA\u3002\u8a72\u8a66\u5291\u76d2\u5229\u7528\u9175\u7d20\u6e2c\u5b9aBCAA\u6c27\u5316\u812b\u6c28 (oxidatively deaminated)\uff0c\u7522\u751fNADH\uff0c\u6e1b\u5c11\u63a2\u91dd\uff0c\u65bc450nm\u6ce2\u9577\u7522\u751f\u6709\u8272\u7522\u7269\uff08\u03bbmax= 450nm\uff09\u3002 BioVision\u7684BCAA\u8a66\u5291\u76d2\u53ef\u6e2c\u91cf\u6bcf\u500b\u6a23\u54c10\u523010 nmol\u7bc4\u570d\u5167\u7684BCAA\uff0c\u6aa2\u6e2c\u9650\u70ba\u301c0.2 nmol\uff08\u6a23\u54c1\u4e2d\u301c10\u03bcM BCAA\uff09\u3002\u652f\u93c8\u6c28\u57fa\u9178\u5b58\u5728\u65bc\u6bcf\u7a2e\u7d040.1-0.4mM\u7684\u8840\u6e05\uff08\u301c0.125-1.5mM)<\/p>\n

\"K564\"<\/a><\/p>\n

\"\"<\/p>\n

\"\"<\/p>\n

\u529f\u80fd\u7814\u7a76 – \u652f\u93c8\u6c28\u57fa\u9178\u6aa2\u6e2c\u8a66\u5291\u76d2 (ab83374)<\/strong><\/span><\/div>\n
\n

\u5728\u5c0f\u9f20\u7d44\u7e54\u88c2\u89e3\u7269\u4e2d\u6e2c\u91cf\u7684 BRCAA \u6c34\u5e73\uff08\u63d0\u53d6\u86cb\u767d\u7684\u6beb\u514b\u6578\uff1b\u6e1b\u53bb\u80cc\u666f\u4fe1\u865f\uff0c\u91cd\u8907\u5e73\u5747\u503c\uff1b+\/- SD\uff09\u3002<\/p>\n

\"\"<\/p>\n

\u529f\u80fd\u7814\u7a76 – \u652f\u93c8\u6c28\u57fa\u9178\u6aa2\u6e2c\u8a66\u5291\u76d2 (ab83374)<\/strong><\/span><\/div>\n
\n

\u5728\u7d30\u80de\u88c2\u89e3\u7269\u4e2d\u6e2c\u91cf\u7684 BRCAA \u6c34\u5e73\uff08\u6e1b\u53bb\u80cc\u666f\u4fe1\u865f\uff0c\u91cd\u8907\u7684\u5e73\u5747\u503c\uff1b+\/- SD\uff09\u3002<\/p>\n

\"\"<\/p>\n

\u529f\u80fd\u7814\u7a76 – \u652f\u93c8\u6c28\u57fa\u9178\u6aa2\u6e2c\u8a66\u5291\u76d2 (ab83374)<\/strong><\/span><\/div>\n
\n

\u5728\u4eba\u9ad4\u751f\u7269\u9ad4\u6db2\u4e2d\u6e2c\u91cf\u7684 BRCAA \u6c34\u5e73\uff08\u6e1b\u53bb\u80cc\u666f\u4fe1\u865f\uff0c\u91cd\u8907\u7684\u5e73\u5747\u503c\uff1b+\/- SD\uff09\u3002<\/p>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK564-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (450 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nBioVision\u2019s BCAA kit measures BCAA\u2019s in the range of 0 to 10 nmol per sample with a detection limit of ~0.2 nmol (~10 \u00b5M BCAA in sample).<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure; takes less than ~40 minutes
\n\u2022 Fast and convenient<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 BCAA Assay Buffer
\n\u2022 BCAA Enzyme Mix
\n\u2022 WST Substrate Mix
\n\u2022 Leu Standard (1 \u00b5mol)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5f15\u7528\u6587\u737b<\/span><\/strong><\/p>\n

\n
Chun-Fang Hsueh, The Effect of Branched-Chain Amino Acids, Citrulline, and Arginine on High-Intensity Interval Performance in Young Swimmers.\u00a0Nutrients, Dec 2018; \u00a030558143.<\/a><\/div>\n<\/div>\n
\n
A. Wilson Aruni\u00a0 et al., VimA-Dependent Modulation of Acetyl Coenzyme A Levels and Lipid A Biosynthesis Can Alter Virulence in\u00a0Porphyromonas gingivalis<\/em>,\u00a0Infect. Immun., Feb 2012; 80: 550 – 564.<\/a><\/div>\n<\/div>\n

\u00a0<\/strong>Sample Preparation:<\/p>\n

Tissue (20 mg) or cells (2 x 106<\/sup>)<\/span> can be homogenized with 100 \u00b5l Assay buffer. Centrifuge at 15,000g for 10 minutes to remove cell debris and other insoluble materials. Add samples to sample wells in a 96-well plate and bring the volume to 50 \u00b5l\/well with Assay Buffer. We suggest testing several doses of your sample to make sure the readings are within the standard curve range. Typical volume for serum samples should be in the range of 1 \u2013 20 \u00b5l.<\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u652f\u93c8\u6c28\u57fa\u9178\uff08Leu \/ Ile \/ Val\uff09\u6bd4\u8272\u5206\u6790\u8a66\u5291\u76d2| \u8ca8\u865fK564-100\u00a0abcam\u8ca8\u865fab8337 […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[468],"tags":[2925],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15520"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=15520"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15520\/revisions"}],"predecessor-version":[{"id":39783,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15520\/revisions\/39783"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=15520"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=15520"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=15520"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}