{"id":15500,"date":"2018-02-05T15:58:51","date_gmt":"2018-02-05T07:58:51","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=15500"},"modified":"2023-07-05T14:38:29","modified_gmt":"2023-07-05T06:38:29","slug":"%e9%86%9b%e8%84%ab%e6%b0%ab%e9%85%b6%e6%b4%bb%e6%80%a7%e6%af%94%e8%89%b2%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92-aldehyde-dehydrogenase-activity-colorimetric-assay-kit%e8%b2%a8%e8%99%9fk731-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=15500","title":{"rendered":"ALDH\u919b\u812b\u6c2b\u9176\u6d3b\u6027\u6aa2\u6e2c | Aldehyde Dehydrogenase Activity Colorimetric Assay Kit\u8ca8\u865fK731-100 (abcam ab155893)"},"content":{"rendered":"

ALDH\u919b\u812b\u6c2b\u9176\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2 <\/strong>| <\/strong>\u8ca8\u865f<\/strong>K731-100 (abcam ab155893)<\/a><\/span><\/p>\n

Aldehyde Dehydrogenase Activity Colorimetric Assay Kit( 0.5 mU\/well)<\/p>\n

\"\"<\/p>\n

NAD\u4f9d\u8cf4\u6027\u919b\u812b\u6c2b\u9176\uff08 NAD-dependent Aldehyde Dehydrogenase, ALDH\uff09\u5728\u7d30\u80de\u89e3\u6bd2 ( cellular detoxification) \u4e2d\u626e\u6f14\u81f3\u95dc\u91cd\u8981\u7684\u4f5c\u7528\u3002\u5b83\u6c27\u5316\u5404\u7a2e\u919b\u985e ( aldehydes) \u4e26\u751f\u6210\u76f8\u61c9\u7684\u7fa7\u9178 (carboxyolic acid\u3002 ALDH\u5df2\u7d93\u5728\u6bcf\u4e00\u500b\u7d30\u80de\u6bd4\u8f03\u4e2d\u627e\u5230\u3002\u6839\u64da\u5176\u7d50\u69cb( structure) \u548c\u529f\u80fd( function)\uff0cALDH\u5728\u54fa\u4e73\u52d5\u7269\u4e2d\u5305\u542b3\u500b\u4e3b\u8981\u985e\u5225\uff1a1\u985e( Class 1) \u548c3\u985e( Class 3) \uff08\u816b\u7624\u5f62\u5f0f\uff09\u4f4d\u65bc\u80de\u8cea (cytosol ) \u6eb6\u81a0\u4e2d\u4e26\u4e14\u5305\u62ec\u7d44\u6210\u578b(constitutive) \u548c\u8a98\u5c0e\u578b(induced forms);\u7b2c2\u985e ( Class 2) \u4f4d\u65bc\u7dda\u7c92\u9ad4( mitochondria ) \u4e2d\uff0c\u50c5\u4f5c\u70ba\u7d44\u6210\u578b\u5b58\u5728(constitutive form) \u3002\u5728\u4eba\u985e\u4e2d\uff0cALDH\u8d85\u5bb6\u65cf (ALDH superfamily ) \u753119\u500b\u57fa\u56e0\u7d44\u6210\u3002 ALDH\u57fa\u56e0\u7684\u7a81\u8b8a\uff08\u529f\u80fd\u55aa\u5931\uff09\u5f15\u8d77\u4eba\u985e\u75be\u75c5\uff0c\u5982II\u578b\u9ad8\u812f\u6c28\u9178\u8840\u75c7 (Type II hyperprolinemia)\uff0c\u5421\u54c6\u9187\u4f9d\u8cf4\u6027\u7672\u7647\u767c\u4f5c(pyridoxine-dependent seizure ) \u548c\u9ad8\u6c28\u8840\u75c7(hyperammonemia) \u3002\u6700\u8fd1\u7684\u7814\u7a76\u986f\u793a\uff0cALDH\u6d3b\u6027\u589e\u52a0\u5c0e\u81f4\u5e7e\u7a2e\u985e\u578b\u7684\u60e1\u6027\u816b\u7624 (malignancies) \uff0c\u4f5c\u70ba\u764c\u75c7\u5e79\u7d30\u80de\u6a19\u8a8c\u7269 (cancer stem cell marker )\u4e26\u8207\u4e0d\u826f\u9810\u5f8c (poor prognosis)\u5177\u95dc\u806f\u6027\u3002\u56e0\u6b64\u65e9\u671f\u6aa2\u6e2cALDH\u6d3b\u6027\u6c34\u5e73\u53ef\u4ee5\u9810\u6e2c\u4e26\u6307\u5c0e\u6cbb\u7642\u7b56\u7565\u3002 BioVision\u919b\u812b\u6c2b\u9176\uff08ALDH\uff09\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2\u662f\u4e00\u7a2e\u7c21\u55ae\uff0c\u5feb\u901f\u548c\u53ef\u9760\u7684\u5b9a\u91cfALDH\u9176\u6d3b\u6027\u7684\u65b9\u6cd5\u3002\u5728\u8a72\u6e2c\u5b9a\u4e2d\uff0c\u4e59\u919b\u88abALDH\u6c27\u5316\uff0c\u7522\u751fNADH\uff0c\u7136\u5f8c\u5c07\u7121\u8272\u63a2\u91dd\u9084\u539f\u6210\u5728450nm\u8655\u5177\u6709\u5f37\u5438\u5149\u5ea6\u7684\u8457\u8272\u7522\u7269\u3002\u8a72\u6e2c\u5b9a\u6cd5\u53ef\u4ee5\u5728\u5404\u7a2e\u6a23\u54c1\u4e2d\u6aa2\u6e2c<0.5mU\u7684ALDH\u6d3b\u6027\uff08\u55ae\u4f4d\u5b9a\u7fa9\u898b\u64cd\u4f5c\u624b\u518a\uff09\u3002<\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK731-100(abcam ab155893)<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (450 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nThe assay can detect < 0.5 mU of ALDH activity (based on our unit definition) in a variety of samples.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure
\n\u2022 Fast and convenient
\n\u2022 Sensitive assay for measuring ALDH activity in various biological samples and the kit is also suitable for high throughput analyses.<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 ALDH Assay Buffer
\n\u2022 Acetaldehyde
\n\u2022 ALDH Substrate Mix (Lyophilized)
\n\u2022 ALDH Positive Control (Lyophilized)
\n\u2022 NADH Standard (0.5 \u00b5mol, Lyophilized)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5f15\u7528\u6587\u737b<\/p>\n

Choi NE et al., (2017) Effects of Cudrania Tricuspidata Root Extract (CTE) on Ethanol-Induced Hangover via Modulating Alcohol Metabolizing Enzyme Activities and Blood Gas Levels in Rats,\u00a0Journal of the Korea Academia-Industrial cooperation Society, 2017, 18(2):218-225<\/a><\/p>\n

\"smart\"<\/a>\"Spectrumlab<\/a>\"102-T\"<\/a>\"0100-01\"<\/a><\/p>\n

\u00a0<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

ALDH\u919b\u812b\u6c2b\u9176\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2 | \u8ca8\u865fK731-100 (abcam ab155893) Aldehyde […]<\/p>\n","protected":false},"author":3,"featured_media":28510,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[2933],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15500"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=15500"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15500\/revisions"}],"predecessor-version":[{"id":19865,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15500\/revisions\/19865"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/28510"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=15500"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=15500"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=15500"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}