{"id":15462,"date":"2018-02-02T13:08:07","date_gmt":"2018-02-02T05:08:07","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=15462"},"modified":"2023-08-24T11:07:34","modified_gmt":"2023-08-24T03:07:34","slug":"%e8%a1%80%e7%ae%a1%e7%94%9f%e6%88%90%ef%bc%88%e7%ae%a1%e5%bd%a2%e6%88%90%ef%bc%89%e5%88%86%e6%9e%90-angiogenesis-tube-formation-assay-%e8%b2%a8%e8%99%9fk905-50","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=15462","title":{"rendered":"\u8840\u7ba1\u751f\u6210\uff08\u7ba1\u5f62\u6210\uff09\u5206\u6790 | Angiogenesis (Tube Formation) Assay \u8ca8\u865fK905-50 (Abcam ab204726)"},"content":{"rendered":"

\u8840\u7ba1\u751f\u6210\uff08\u7ba1\u5f62\u6210\uff09\u5206\u6790 <\/strong>| <\/strong>\u8ca8\u865f<\/strong>K905-50<\/a>\u00a0(Abcam ab204726)<\/span><\/p>\n

Angiogenesis (Tube Formation) Assay<\/em><\/strong><\/p>\n

\"\"\"\"<\/p>\n

\u8840\u7ba1\u751f\u6210(Angiogenesis)\u662f\u5f9e\u9810\u5148\u5b58\u5728\u7684\u8108\u7ba1\u7cfb\u7d71\u7522\u751f\u65b0\u8840\u7ba1(new blood vessels )\u7684\u904e\u7a0b\uff0c\u662f\u751f\u9577(growth)\u548c\u767c\u80b2(development)\u3001\u50b7\u53e3\u7652\u5408(wound healing)\u3001\u7d44\u7e54\u8089\u82bd\u751f\u6210(tissue granulation)\u548c\u60e1\u6027\u816b\u7624(malignant tumors)\u5f62\u6210\u6240\u5fc5\u9700\u7684\u3002 \u8840\u7ba1\u751f\u6210(\u00a0angiogenesis i)\u7684\u5feb\u901f\u8a55\u4f30\u5305\u62ec\u6e2c\u91cf\u5167\u76ae\u7d30\u80de\u5f62\u6210\u4e09\u7dad\u7ba1\u72c0\u7d50\u69cb(\u00a0three-dimensional tube-like structures)\u7684\u80fd\u529b\u3002\u8840\u7ba1\u65b0\u751f\u4f5c\u7528\u662f\u7531\u5167\u76ae\u7d30\u80de(endothelial cells)\u6240\u5f15\u5c0e\u7684\uff0c\u904e\u7a0b\u4e2d\u7d30\u80de\u6703\u5206\u6ccc\u9175\u7d20\u4f86\u5206\u89e3\u57fa\u5e95\u819c\uff0c\u9020\u6210\u7d30\u80de\u4e4b\u904a\u8d70\uff0c\u7d93\u7531\u4e00\u9023\u4e32\u7684\u589e\u751f\u5206\u5316\u5f8c\uff0c\u6700\u5f8c\u5f62\u6210\u7ba1\u72c0\u822c\u7684\u8840\u7ba1\u69cb\u9020\u3002 BioVision\u7684\u8840\u7ba1\u5f62\u6210\u8a66\u9a57(\u00a0Tube Formation Assay, K905)\u63d0\u4f9b\u4e86\u4e00\u500b\u5f37\u5927\u7684\u65b9\u6cd5\u4f86\u78ba\u5b9a\u8840\u7ba1\u751f\u6210\uff08\u00a0angiogenesis) (in vitro\uff09\u4e0d\u523018\u5c0f\u6642\u3002 \u8a72\u6aa2\u6e2c\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u8a55\u4f30\u8840\u7ba1\u751f\u6210\u7684\u7c21\u55ae\uff0c\u6613\u65bc\u57f7\u884c\u7684\u534a\u5b9a\u91cf\u5de5\u5177\u3002\u53ef\u4ee5\u5075\u6e2c\u8840\u7ba1\u7d30\u80de\u8840\u7ba1\u5f62\u6210\u4e4binducers\u53cainhibitors\u5206\u6790\u3002<\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

Extracellular Matrix Solution 2 X 1.25 ml<\/p>\n

Wash Buffer 10 ml<\/p>\n

Staining Dye Concentrate 25 \u00b5l<\/p>\n

Inhibitor Control -Vinblastine (2 \u00b5M) 10 \u00b5l<\/p>\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK905-50 (Abcam ab204726)<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n50 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nLight & Fluorescence microscope<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nScreening inhibitors and stimulators of angiogenesis
\nStudy of angiogenesis related signal transduction<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Robust method to determine angiogenesis (in vitro) in less than 18 hrs
\n\u2022 Simple & convenient
\n\u2022 semi-quantitative tool for assessing angiogenesis<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Extracellular Matrix Solution (2 vials)
\n\u2022 Wash Buffer
\n\u2022 Staining Dye Concentrate
\n\u2022 Inhibitor Control -Vinblastine (2 \u00b5M)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Extracellular Matrix Solution\u7d30\u80de\u5916\u57fa\u8cea\u6eb6\u6db2\uff1a\u9577\u671f\uff086\u500b\u6708\uff09\u5132\u5b58\uff0c\u6211\u5011\u5efa\u8b70\u5728\u7121\u83cc\u689d\u4ef6\u4e0b\u5206\u5e7e\u6b21\u4e26\u5728-20\u00b0C\u5132\u5b58\u3002 \u907f\u514d\u51cd\u7d50\u89e3\u51cd\u5faa\u74b0\u3002 \u59cb\u7d42\u5728\u51b0\u4e0a\u62164\u00b0C\u7121\u971c\u51b0\u7bb1\u4e2d\u7de9\u6162\u878d\u5316\u3002 \u6eab\u5ea6\u9ad8\u65bc4\u00b0C\u6703\u8fc5\u901f\u81a0\u51dd\u80de\u5916\u57fa\u8cea\u6eb6\u6db2\u3002 \u89e3\u51cd\u53ef\u80fd\u9700\u8981\u57284\u00b0C\u904e\u591c\u3002 \u89e3\u51cd\u7684\u77e9\u9663\u53ef\u4ee5\u5b58\u5132\u57282-8\u00b0C\u4e00\u500b\u661f\u671f\u3002Inhibitor Control\u6291\u5236\u5291\u63a7\u5236\u7d44 – -Vinblastine\u9577\u6625\u9e7c\uff1a-20\u00b0C\u4fdd\u5b58\u3002 \u7528\u5c0d\u7167 – \u9577\u6625\u9e7c\u9032\u884c\u7684\u8655\u7406\u5c07\u6839\u64da\u7d30\u80de\u985e\u578b\u800c\u8b8a\u5316\u3002 \u5167\u76ae\u7d30\u80de\u7cfb\uff08EA.hy926\u7d30\u80de\uff09\uff0c\u63a8\u85a6\u4f7f\u7528\u7d42\u6fc3\u5ea61 pmol \/ L\u3002 \u5728Wash\u4e2d\u7a00\u91cbVinblastin\u00a0\u3002<\/p>\n

\"\u5176\u5b83\u4e8b\u9805\"<\/strong><\/p>\n

\u8840\u7ba1\u751f\u6210\uff08\u7ba1\u5f62)\u5206\u6790\u64cd\u4f5c\u4ecb\u7d39<\/strong><\/p>\n

\u7d30\u80de\u57f9\u990a\uff1a\u5c07\u57f9\u990a\u7684\u5167\u76ae\u7d30\u80de\u4ee5\u6240\u9700\u57f9\u990a\u57fa\u57f9\u990a\u81f3\u301c90\uff05\u5bc6\u5ea6\uff08\u542b\u67095\uff05CO 2\u768437\u2103\u57f9\u990a\u7bb1\uff09\u3002\u7121\u83cc\u689d\u4ef6\u4e0b\u4e0b\u6536\u7a6b\u7d30\u80de\u3002\u4e26\u65bc\u5728\u542b\u67090.5-5\uff05\u8840\u6e05\u7684\u6240\u9700\u57f9\u990a\u57fa\u4e2dResuspend\u7d30\u80de\u3002<\/p>\n

\u8840\u7ba1\u751f\u6210\uff1a\u9810\u51b7\u7684\uff08\u5728\u51b0\u4e0a\uff0996\u5b54\u7121\u83cc\u7d30\u80de\u57f9\u990a\u677f\u6bcf\u5b54\u4e2d\u52a0\u516550\u03bcl\u89e3\u51cd\u7684\u7d30\u80de\u5916\u57fa\u8cea\u6eb6\u6db2\u3002\u78ba\u4fdd\u51dd\u81a0\u5728\u4e95\u58c1\u8868\u9762\u5747\u52fb\u5206\u4f48\uff08\u8f15\u8f15\u6416\u52d5\u6216\u8f15\u6572\u4ee5\u5c55\u958b\uff09\u3002 37\u00b0C\u5b75\u80b21\u5c0f\u6642\uff0c\u4f7f\u6eb6\u6db2\u5f62\u6210\u51dd\u81a0\u3002\u4f7f\u7528\u5927\u7d041-2\u00d710 4\u00a0\u5167\u76ae\u7d30\u80de<\/sup>\/100\u03bcl\u57f9\u990a\u57fa\/\u5b54\u3002\u5c07\u7d30\u80de\u6dfb\u52a0\u5230\u51dd\u56fa\u7269\u4e0a\u7d30\u80de\u5916\u57fa\u8cea\u51dd\u81a0\u6216\u5c0d\u7167\u5b54\uff08\u7121\u7d30\u80de\u5916\u57fa\u8cea\u51dd\u81a0\u6216\u5177\u6709\u9577\u6625\u9e7c\u7684\u7d30\u80de\u5916\u57fa\u8cea\u5b54\uff09\u3002\u6dfb\u52a0\u8840\u7ba1\u751f\u6210\u56e0\u7d20\/\u8abf\u7bc0\u5668\u5230\u6240\u9700Well\u3002\u5728\u542b\u67095\uff05CO2\u768437\u2103\u57f9\u990a\u7bb1\u4e2d\u57f9\u990a\u7d30\u80de4-18\u5c0f\u6642\u3002<\/p>\n

\u6578\u64da\u5206\u6790\uff1a\u4f7f\u7528\u00a0pipette\u5c0f\u5fc3\u79fb\u9664\u57f9\u990a\u57fa\u800c\u4e0d\u5e72\u64fe\u7d30\u80de\u6216\u7d30\u80de\u5916\u57fa\u8cea\u51dd\u81a0\u3002\u8f15\u8f15\u5730\u6d17\u7528100\u03bcl\u6d17\u6ecc\u7de9\u885d\u6db2\u6e05\u9664\u8840\u6e05\u3002\u5c0f\u5fc3\u5730\u53d6\u51fa\u6e05\u6d17\u7de9\u885d\u6db2\u3002\u6e96\u5099100\u03bcl\/\u5b54\u67d3\u8272\u67d3\u6599\u5de5\u4f5c<\/p>\n

\u5c07\u67d3\u8272\u6fc3\u7e2e\u72691\uff1a200\uff08\u4f8b\u59825\u03bcl\u67d3\u8272\u6fc3\u7e2e\u7269\u5728995\u03bcl\u6d17\u6ecc\u7de9\u885d\u6db2\u4e2d\uff09\u6309\u7167\u4f7f\u7528well\u6578\u3002\u6bcf\u5b54\u52a0\u5165100\u03bclStaining Dye\u5de5\u4f5c\u6eb6\u6db2\u3002\u5b75\u80b230\u5206\u9418\u3002\u572837\u2103\u4e0b\u3002\u6aa2\u67e5\u5167\u76ae\u7ba1\u4f7f\u7528\u5149\u548c\u87a2\u5149\u986f\u5fae\u93e1\uff08\u7da0\u8272\u904e\u6ffe\u5668\uff09\u5f62\u6210\u3002\u6211\u5011\u5efa\u8b70\u6bcf\u500bwell\u6536\u96c6\u5e7e\u5f35\u5716\u50cf\u3002\u6709\u5e7e\u500b\u9078\u9805\u53ef\u7528\u65bc\u5206\u6790\u5716\u7247\u3002\u5c0d\u65bc\u624b\u52d5\u5206\u6790\uff0c\u6211\u5011\u63a8\u85a6\u4f7f\u7528\u5716\u50cf\u8655\u7406\u8edf\u4ef6\u3002\u5716\u7247J\uff08\u5f9eNIH\u514d\u8cbb\uff09\u3002\u5c0d\u65bc\u81ea\u52d5\u5206\u6790\uff0c\u6211\u5011\u63a8\u85a6\u4f7f\u7528WimTube\u5716\u50cf\u5206\u6790\u5de5\u5177\u3002\u5b83\u57fa\u65bc\u5c0f\u7ba1\u7279\u5fb5\u5373\u5c0f\u7ba1\u7684\u6578\u91cf\uff0c\u9023\u63a5\u7684\u6578\u91cf\uff0c\u5c0f\u7ba1\u9577\u5ea6\u548c\u74b0\u7684\u6578\u91cf\u3002<\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u8840\u7ba1\u751f\u6210\uff08\u7ba1\u5f62\u6210\uff09\u5206\u6790 | \u8ca8\u865fK905-50\u00a0(Abcam ab204726) Angiogenesis ( […]<\/p>\n","protected":false},"author":3,"featured_media":28499,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[126],"tags":[2945],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15462"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=15462"}],"version-history":[{"count":6,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15462\/revisions"}],"predecessor-version":[{"id":15466,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15462\/revisions\/15466"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/28499"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=15462"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=15462"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=15462"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}