{"id":15455,"date":"2018-02-02T10:23:16","date_gmt":"2018-02-02T02:23:16","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=15455"},"modified":"2022-07-20T17:49:12","modified_gmt":"2022-07-20T09:49:12","slug":"%e5%90%b2%e5%93%9a%e8%83%ba23-%e9%9b%99%e5%8a%a0%e6%b0%a7%e9%85%b61%ef%bc%88ido1%ef%bc%89%e6%b4%bb%e6%80%a7%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fk972-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=15455","title":{"rendered":"\u5432\u54da\u80fa2,3-\u96d9\u52a0\u6c27\u91761\uff08IDO1\uff09\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2 | \u8ca8\u865fK972-100"},"content":{"rendered":"<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=15455\"><strong>\u5432\u54da\u80fa2,3-\u96d9\u52a0\u6c27\u91761\uff08IDO1\uff09\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2 <\/strong><strong>| <\/strong><strong>\u8ca8\u865fK972-100<\/strong><\/a><\/span><\/p>\n<p><strong><em>Highly Sensitive; Includes recombinant IDO1 enzyme as positive control.<\/em><\/strong><\/p>\n<p><strong>\u7522\u54c1<\/strong><strong>\u63cf\u8ff0\uff1a<\/strong>\u5432\u54da\u80fa2,3-\u96d9\u52a0\u6c27\u91761\uff08IDO1\uff0cEC 1.13.11.52\uff09\u662f\u6c27\u5316\u8272\u6c28\u9178\u7522\u751fN-\u7532\u9170\u72ac\u5c3f\u6c28\u9178\uff08NFK\uff09\u7684\u7d30\u80de\u8cea\u8840\u7d05\u86cb\u767d\u3002\u5728\u54fa\u4e73\u52d5\u7269\u4e2d\uff0c\u9019\u7a2e\u53cd\u61c9\u662f\u72ac\u5c3f\u6c28\u9178\u5206\u89e3\u4ee3\u8b1d\u9014\u5f91\u4e2d\u7684\u7b2c\u4e00\u6b65\u548c\u9650\u901f\u6b65\u9a5f\u3002\u5728\u6b63\u5e38\u751f\u7406\u689d\u4ef6\u4e0bIDO1\u6d3b\u6027\u4f4e\uff0c\u4f46\u662f\u901a\u904e\u4e7e\u64fe\u7d20-\u03b3\u7b49\u4fc3\u708e\u7d30\u80de\u56e0\u5b50\u986f\u8457\u4e0a\u8abf\u3002\u9019\u7a2eIDO1\u7684\u77ed\u671f\u6fc0\u6d3b\u4f5c\u70ba\u5148\u5929\u514d\u75ab\u53cd\u61c9\u7684\u4e00\u90e8\u5206\u767c\u751f\uff0c\u4e26\u6709\u52a9\u65bc\u6291\u5236\u75c5\u539f\u9ad4\u548c\u5bc4\u751f\u87f2\u7684\u751f\u9577\u3002 IDO1\u6fc0\u6d3b\u4e5f\u53ef\u4ee5\u901a\u904e\u767c\u63ee\u514d\u75ab\u6291\u5236\u4f5c\u7528\u4f86\u4fc3\u9032\u5bbf\u4e3b\u514d\u75ab\u8010\u53d7\u3002\u816b\u7624\u7d30\u80de\u8868\u9054IDO1\u5728\u816b\u7624\u514d\u75ab\u8010\u53d7\u4e2d\u8d77\u91cd\u8981\u4f5c\u7528\uff0c\u6709\u52a9\u65bc\u816b\u7624\u7684\u9003\u907f\u6aa2\u6e2c\u548c\u7834\u58de\u3002\u5728\u764c\u75c7\u60a3\u8005\u4e2d\u767c\u73feIDO1\u8868\u9054\u7684\u6162\u6027\u8a98\u5c0e\u589e\u52a0\uff0cIDO1\u6d3b\u6027\u8207\u6d88\u6975\u9810\u5f8c\u76f8\u95dc\u3002\u56e0\u6b64\uff0cIDO1\u6210\u70ba\u958b\u767c\u65b0\u578b\u6297\u816b\u7624\u85e5\u7269\u548c\u4f50\u5291\u4ee5\u63d0\u9ad8\u5e38\u898f\u5316\u7642\u7642\u6548\u7684\u6709\u5438\u5f15\u529b\u7684\u85e5\u7406\u5b78\u9776\u9ede\u3002 BioVision\u7684IDO1\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2\u53ef\u4ee5\u5728\u54fa\u4e73\u52d5\u7269\u7d44\u7e54\u548c\u7d30\u80de\u7cfb\u4e2d\u8f15\u9b06\u78ba\u5b9aIDO1\u7684\u6d3b\u6027\u3002\u8a72\u6e2c\u5b9a\u4f7f\u7528\u87a2\u5149\u986f\u5f71\u5291\uff0c\u5176\u9078\u64c7\u6027\u5730\u8207NFK\u53cd\u61c9\u7522\u751f\u9ad8\u5ea6\u87a2\u5149\u7684\u7522\u7269\uff08Ex \/ Em = 402 \/ 488nm\uff09\uff0c\u78ba\u4fdd\u9ad8\u4fe1\u865f\u80cc\u666f\u6bd4\u3002\u8a72\u8a66\u5291\u76d2\u9084\u5305\u62ec\u4e00\u500b\u9ad8\u5ea6\u9078\u64c7\u6027\u7684IDO1\u6291\u5236\u5291\uff0c\u7528\u65bc\u9a57\u8b49\u751f\u7269\u57fa\u8cea\u4e2d\u7684\u9176\u6d3b\u6027\u3002\u8a72\u8a66\u5291\u76d2\u5177\u6709\u7c21\u55ae\u7684\u514d\u6d17\u65b9\u6848\uff0c\u5177\u6709\u9ad8\u901a\u91cf\u9069\u61c9\u6027\uff0c\u53ef\u6aa2\u6e2c\u52300.2 mU\u7684IDO1\u6d3b\u6027\u6216200 pmole\u7684NFK\u3002<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/www.biovision.com\/media\/catalog\/product\/cache\/1\/image\/650x\/040ec09b1e35df139433887a97daa66f\/k\/9\/k972.jpg\" alt=\"\" \/><\/p>\n<table width=\"838\">\n<tbody>\n<tr>\n<td><strong>Cat # +Size<\/strong><\/td>\n<td>K972-100<\/td>\n<\/tr>\n<tr>\n<td><strong>Size<\/strong><\/td>\n<td>100 assays<\/td>\n<\/tr>\n<tr>\n<td><strong>Detection Method<\/strong><\/td>\n<td>Fluorescence (Ex\/Em 402\/488 nm)<\/td>\n<\/tr>\n<tr>\n<td><strong>Species Reactivity<\/strong><\/td>\n<td>Mammalian<\/td>\n<\/tr>\n<tr>\n<td><strong>Applications<\/strong><\/td>\n<td>Rapid assessment of native\/recombinant IDO1 activity in lysates prepared from tissues, cells.<br \/>\nScreening of drugs and novel ligands for interaction with native\/recombinant IDO1.<\/td>\n<\/tr>\n<tr>\n<td><strong>Features &amp; Benefits<\/strong><\/td>\n<td>\u2022 Simple, convenient, highly sensitive \u2022 Fluorescent assay enables easy determination of IDO1 activity in a variety of biological samples \u2022 The substrate shows low background and a high signal-to-noise ratio \u2022 Kit includes IDO1 inhibitor, IDO5L, and a stable, recombinant human IDO1 enzyme as a positive control<\/td>\n<\/tr>\n<tr>\n<td><strong>Kit Components<\/strong><\/td>\n<td>\u2022 IDO1 Assay Buffer<br \/>\n\u2022 Antioxidant Mix (100X)<br \/>\n\u2022 N-formylkynurenine Standard<br \/>\n\u2022 IDO1 Substrate (L-tryptophan)<br \/>\n\u2022 IDO Inhibitor (IDO5L)<br \/>\n\u2022 Fluorogenic Developer Solution \u2022 Recombinant Human IDO1<br \/>\n\u2022 Microplate Sealing Film<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u00a0<\/strong><\/p>\n<p><strong>\u00a0<\/strong><\/p>\n<p><strong>\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u5432\u54da\u80fa2,3-\u96d9\u52a0\u6c27\u91761\uff08IDO1\uff09\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2 | \u8ca8\u865fK972-100 Highly Sensitive [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[1,3],"tags":[2946,2512],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15455"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=15455"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15455\/revisions"}],"predecessor-version":[{"id":15459,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15455\/revisions\/15459"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=15455"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=15455"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=15455"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}