{"id":15430,"date":"2018-01-31T13:17:18","date_gmt":"2018-01-31T05:17:18","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=15430"},"modified":"2024-06-04T10:53:24","modified_gmt":"2024-06-04T02:53:24","slug":"%e6%a0%b8%e7%b3%96%e6%a0%b8%e9%85%b8%e9%85%b6r-dna-rna-modifying-enzymes","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=15430","title":{"rendered":"\u6838\u7cd6\u6838\u9178\u9176RNase R (NGS) | DNA & RNA Modifying Enzymes\u8ca8\u865fE049"},"content":{"rendered":"

\u6838\u7cd6\u6838\u9178\u9176<\/span><\/strong>R<\/strong> | DNA & RNA Modifying Enzymes \u8ca8\u865fE049<\/strong><\/span><\/a>\u00a0500 U (50 \u03bcl)\u00a0 10U\/ul\u00a0<\/span><\/p>\n

RNase R\u00a0\u00a0<\/strong><\/span><\/p>\n

\u6838\u7cd6\u6838\u9178\u9176R (RNase R )\u662f\u4e00\u7a2e\u5927\u8178\u687f\u83cc\u6838\u7cd6\u6838\u9178\u5916\u5207\u9176\u00a0( E. coli exoribonuclease)\uff0c\u5177\u67093’\u81f35’\u6838\u9178\u5916\u5207\u9176\u6d3b\u6027\uff0c\u80fd\u5920\u6709\u6548\u5730\u6d88\u5316\u5e7e\u4e4e\u6240\u6709\u7684\u7dda\u6027RNA (\u00a0linear RNA) \u7a2e\u985e\u548cY\u7d50\u69cbRNA\u3002 \u8a72\u9176\u4e0d\u80fd\u6d88\u5316\u5177\u6709\u77ed3’\u7a81\u51fa\u7aef\uff08\u5c0f\u65bc7\u500b\u6838\u82f7\u9178\uff09\u7684\u74b0\u72c0 (\u00a0circular) \uff0c\u5957\u7d22\u72c0(lariat)\u6216\u96d9\u93c8(double stranded) RNA\uff0c\u4fdd\u7559\u5957\u7d22RNA\u7684\u74b0\u90e8\u5206\u3002 \u56e0\u6b64\uff0c\u9019\u7a2e\u9176\u975e\u5e38\u9069\u5408\u65bc\u7814\u7a76\u7531\u50b3\u7d71\u526a\u63a5\u7522\u751f\u7684\u5957\u7d22RNA(\u00a0circRNAs)\uff0c\u4ee5\u53ca\u901a\u904e\u53cd\u5411\u526a\u63a5\u7522\u751f\u7684circRNA\u3002 RNase R\u901a\u904e\u5f9e\u7d30\u80de\u6216RNA\u63d0\u53d6\u7269\u4e2d\u53bb\u9664\u7dda\u6027RNA\uff0cRNase R\u7d93\u7531RNA-sequencing\u6709\u6548\u9451\u5b9a\u74b0\u72c0\u7269\u7a2e\u3002<\/p>\n

Enriching circRNAs in biological samples
\n\u2022 Identification of intronic lariat sequences
\n\u2022 Identification of exonic circRNAs
\n\u2022 Studying alternative splicing
\n\u2022 Production of artificial circular RNAs<\/p>\n

<\/p>\n

HOT!!<\/strong>\u00a0<\/u>\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c<\/span><\/a>\u00a0|<\/strong>\u00a0DAPI\u67d3\u6838<\/a>\u00a0|<\/strong>\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291<\/a>\u00a0|<\/strong>\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291<\/a>\u00a0|<\/strong>\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49\u7cfb\u5217\u5546\u54c1<\/a><\/span><\/span><\/p>\n\n\n\n\n\n\n
Concentration<\/td>\n10 U\/\u03bcl<\/td>\n<\/tr>\n
Storage<\/td>\nStore all components at -20 \u00b0C. Avoid repeated freeze-thaw cycles of all components to retain maximum performance. All components are stable for 1 year from the date of shipping when stored and handled properly.<\/td>\n<\/tr>\n
Storage Buffer<\/td>\n50 mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1 mM EDTA, 1 mM DTT, and 50% (v\/v) Glycerol.<\/td>\n<\/tr>\n
Reaction Buffer<\/td>\n200 mM Tris-HCl, 1 M KCl, 1 mM MgCl2, pH 7.5<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

RNase R\u9175\u7d20\u4f7f\u7528\u6ce8\u610f\u4e8b\u9805<\/strong><\/span><\/p>\n

\u5982\u679c\u964d\u89e3\u6548\u7387\u4f4e\uff0c\u5247\u4f7f\u7528\u7a0d\u9ad8\u7684\u5b75\u80b2\u6eab\u5ea6\uff0840-45\u00b0C\uff09\u5728\u4e2d\u9014\u88dc\u5145\u984d\u5916\u7684\u9176\uff080.5\u03bcl 1\u5c0f\u6642\uff09\u3002\u8f03\u9ad8\u7684\u6eab\u5ea6\u5c0d\u65bc\u964d\u89e3\u9ad8\u5ea6\u7d50\u69cb\u5316\u7684\u7dda\u6027RNA\uff08\u4f8b\u5982rRNA\uff09\u7279\u5225\u6709\u7528\u3002\u8d85\u904e45\u00b0C\u6216\u5b75\u80b2\u8d85\u904e3\u5c0f\u6642\uff0c\u4ea6\u53ef\u80fd\u5c0e\u81f4\u975e\u9176\u4fc3RNA\u964d\u89e3\u3002\u6700\u4f73\u6d3b\u6027\u9700\u8981\u6fc3\u5ea6\u70ba0.1-1.0mM\u7684\u9382 (\u00a0Magnesium )\u3002\u5982\u679c\u5b58\u5728EDTA\uff0c\u5247\u901a\u904e\u6dfb\u52a0MgCl 2\u81f3\u6700\u7d421.0mM\u9032\u884c\u88dc\u511f\u3002RNase R\u5c0dtRNA\uff0crRNA\u548c\u5176\u4ed6\u9ad8\u5ea6\u7d50\u69cb\u5316\u7684RNA\u8868\u73fe\u51fa\u4f4e\u6d3b\u6027\uff0c\u51763’\u672b\u7aef\u662f\u96d9\u93c8\u7684\uff0c\u5177\u6709\u77ed\u76843’\u7a81\u51fa\u7aef\u3002\u9019\u4e9bRNA\u7a2e\u985e\u53ef\u4f7f\u9176\u505c\u6eef\u4e26\u5c0e\u81f4\u6d3b\u6027\u5927\u5927\u964d\u4f4e\u3002\u70ba\u7372\u5f97\u6700\u4f73\u7d50\u679c\uff0c\u5f37\u70c8\u5efa\u8b70\u5f9e\u7e3dRNA\u63d0\u53d6\u7269\u4e2d\u53bb\u9664rRNA\u3002\u5c0d\u65bc\u7e3dRNA\u7684\u6d88\u5316\uff0c\u901a\u5e38\u9700\u89812-3\u5c0f\u6642\u7684\u66f4\u9577\u6642\u9593\u7684incubations\u3002<\/p>\n

One unit<\/strong>\u5b9a\u7fa9<\/strong>\u70ba\u572837\u2103\u4e0b\u572810\u5206\u9418\u5167\u5c071\u03bcg poly(A)\u00a0\u8f49\u5316\u70ba\u9178\u6eb6\u6027\u6838\u82f7\u9178 (\u00a0acid-soluble nucleotides)\u7684RNase R\u7684\u91cf\u3002<\/p>\n

<\/p>\n

\"\"<\/p>\n

<\/p>\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

\u6838\u7cd6\u6838\u9178\u9176R | DNA & RNA Modifying Enzymes \u8ca8\u865fE049\u00a0500 U ( […]<\/p>\n","protected":false},"author":3,"featured_media":8880,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5373,88],"tags":[2950],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15430"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=15430"}],"version-history":[{"count":7,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15430\/revisions"}],"predecessor-version":[{"id":49547,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15430\/revisions\/49547"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/8880"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=15430"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=15430"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=15430"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}