{"id":15367,"date":"2018-01-18T12:59:17","date_gmt":"2018-01-18T04:59:17","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=15367"},"modified":"2022-07-20T17:51:04","modified_gmt":"2022-07-20T09:51:04","slug":"scrambled-sgrna-crispr-lentivirus-%e8%b2%a8%e8%99%9fk018","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=15367","title":{"rendered":"Scrambled sgRNA CRISPR Lentivirus | \u8ca8\u865fK018"},"content":{"rendered":"

Scrambled sgRNA CRISPR Lentivirus<\/strong> | <\/strong>\u8ca8\u865f<\/strong>K018<\/a><\/span><\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>: \u00a0<\/strong>Abmgood\u63d0\u4f9bsgRNA-CRISPR Lentivirus, \u8d85\u904e90\uff05\u7684\u69cb\u5efa\u9ad4\u5c07\u572820-90\uff05\u7684\u7d30\u80de\u4e2d\u5be6\u73fe\u5b8c\u5168\u6572\u9664\u3002 \u5927\u591a\u6578\u69cb\u5efa\u9ad4> 50\uff05 \u00a0transduced\u8f49\u5c0e\u548cselected cells\u3002 \u8d8a\u4f86\u8d8a\u591a\u7684\u8b49\u64da\u8868\u660e\uff0csgRNA\u6bd4shRNA\u548csiRNA\u6572\u9664\u6a5f\u7387\u9ad8\u3002\u5982\u679c\u8a2d\u8a08\u7528\u65bcCas9\u6838\u9178\u9176\u7684sgRNA\u69cb\u5efa\u9ad4\u6c92\u6709\u5408\u7406\u7684\u6572\u9664\uff0c\u5c07\u514d\u8cbb\u8a2d\u8a08\u53e6\u4e00\u7d443\u500b\u69cb\u5efa\u9ad4\u3002\u9700\u78ba\u4fdd\u60a8\u76e1\u53ef\u80fd\u5730\u512a\u5316\u4e86\u60a8\u7684\u5be6\u9a57\uff0c\u5305\u62ec\uff08\u5982\u9069\u7528\uff09\u589e\u52a0\u60a8\u7684MOI\uff08\u6700\u591a10\u6b21\uff09\u548c\u611f\u67d3\u6301\u7e8c\u6642\u9593\uff08\u6700\u591a72\u5c0f\u6642\uff09\uff0c\u4e26\u5728\u9032\u884cclone screening\u514b\u9686\u7be9\u9078\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
roduct Application<\/strong><\/td>\n\n

\u00b7\u00a0 Direct non-viral plasmid transfection for immediate expression<\/p>\n

\u00b7\u00a0 Package into Lentiviral particles for high efficiency transduction and stably integrated expression<\/td>\n<\/tr>\n

Species<\/strong><\/td>\nN\/A<\/td>\n<\/tr>\n
Vector<\/strong><\/td>\npLenti-U6-sgRNA-PGK-Neo<\/a><\/td>\n<\/tr>\n
Target Sequence<\/strong><\/td>\nGCACTCACATCGCTACATCA<\/td>\n<\/tr>\n
Format<\/strong><\/td>\nPlasmid<\/td>\n<\/tr>\n
Appearance<\/strong><\/td>\nLiquid<\/td>\n<\/tr>\n
Storage<\/strong><\/td>\n-20\u00b0C or below<\/td>\n<\/tr>\n
Shelf Life<\/strong><\/td>\n1 year (when at -20\u00b0C or below in a non-frost free freezer)<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nShipped at ambient temperature<\/td>\n<\/tr>\n
Additional Materials<\/strong><\/td>\n–\u00a0CRISPR Genomic Cleavage Detection Kit (G932)<\/a><\/td>\n<\/tr>\n
Quality Control<\/strong><\/td>\nRestriction Enzyme Digest and Sequencing<\/td>\n<\/tr>\n
Caution<\/strong><\/td>\nThis product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.<\/td>\n<\/tr>\n
Note<\/strong><\/td>\nabm<\/strong>‘s sgRNAs are designed for use with Cas9 Nuclease only. Please inquire for custom sgRNAs for purposes other than genome editing (e.g. CRISPRa). For CRISPR-Cas9 system applications in genome regulation, please see our comprehensive collection of\u00a0Cas9 mutant proteins (Nickase, Null mutant)<\/a>.<\/td>\n<\/tr>\n
Disclaimer<\/strong><\/td>\n1. The end user acknowledges that the Materials provided under\u00a0abm<\/strong>\u2019s Material Transfer Agreement (MTA) does not grant a license for commercial use or imply any ownership rights of any intellectual property rights relating to the Materials.<\/td>\n<\/tr>\n
Species<\/strong><\/td>\nN\/A<\/td>\n<\/tr>\n
Vector<\/strong><\/td>\npLenti-U6-sgRNA-PGK-Neo<\/a><\/td>\n<\/tr>\n
Target Sequence<\/strong><\/td>\nGCACTCACATCGCTACATCA<\/td>\n<\/tr>\n
Format<\/strong><\/td>\nVirus<\/td>\n<\/tr>\n
Appearance<\/strong><\/td>\nLiquid<\/td>\n<\/tr>\n
Storage<\/strong><\/td>\nLentiviruses are shipped with dry ice. For long term storage, it is recommended to store the viruses at -80\u00b0C in small aliquots to avoid repeated freeze-thaw cycles.<\/td>\n<\/tr>\n
Shelf Life<\/strong><\/td>\n1 year (when at -80\u00b0C or below in a non-frost free freezer)<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nShipped with dry ice<\/td>\n<\/tr>\n
Additional Materials<\/strong><\/td>\n–\u00a0CRISPR Genomic Cleavage Detection Kit (G932)<\/a><\/td>\n<\/tr>\n
Quality Control<\/strong><\/td>\nRestriction Enzyme Digest and Sequencing<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

HOT \u6162\u75c5\u6bd2\uff08Lentivirus\uff09\u8868\u73fe\u6700\u4f73\u5316\u00a0\u00a0<\/strong><\/span>\u6539\u826f\u5f0f\u5305\u88dd\u7cfb\u7d71 | \u75c5\u6bd2\u7d14\u5316\u8207\u6fc3\u7e2e | \u75c5\u6bd2\u529b\u50f9\u5b9a\u91cf\u7cfb\u5217\u5546\u54c1\u00a0<\/strong><\/span>\u63d0\u6607\u6162\u75c5\u6bd2\u611f\u67d3\u529b<\/strong><\/span><\/span><\/p>\n

\u6162\u75c5\u6bd2\uff08Lentivirus\uff09\u7684\u7d50\u69cb\u53ef\u5206\u6210\u5957\u819c\uff08envelop\uff09\u3001\u86cb\u767d\u8cea\u819c\uff08capsid\uff09\u3001\u4ee5\u53ca\u907a\u50b3\u7269\u8cea\u3002Lentivirus\u611f\u67d3\u5bbf\u4e3b\u5f8c\uff0c\u6703\u5c07\u5176\u57fa\u56e0\u5d4c\u5165\u5bbf\u4e3b\u7d30\u80de\u4f7f\u5176\u8868\u9054\u3002\u73fe\u70ba\u5be6\u9a57\u5ba4\u4e2d\u5e38\u898b\u7684\u57fa\u56e0\u91cd\u7d44\u5de5\u5177\u3002lentivirus\u5df2\u53ef\u85c9\u7531\u30001. \u5e36\u6709target gene\u7684lentivector\uff0c\u52a0\u4e0a\u30002. packaging system\uff0c<\/span>\u69cb\u6210\u7121\u81f4\u75c5\u6027\u7684psuedovirus\u3002<\/p>\n

Lentivirus\u6162\u75c5\u6bd2\u4f4e\u901f30\u5206\u7d14\u5316\u5957\u7d44(\u7d14\u5316)<\/a>|\u00a0Lentivirus \u6162\u75c5\u6bd2\u9ad8\u6fc3\u5ea6\u7d14\u5316(\u7d14\u5316)\u00a0<\/a>|\u00a0Lentivirus\u75c5\u6bd2RT-QPCR\u5b9a\u91cf(\u5b9a\u91cf)<\/a>\u00a0|\u00a0Lentivirus\u75c5\u6bd2\u5b9a\u91cf\u5feb\u7be9\u7247(\u5b9a\u91cf\u5361)<\/a><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

Scrambled sgRNA CRISPR Lentivirus | \u8ca8\u865fK018 \u7522\u54c1\u4ecb\u7d39: \u00a0Abmgo […]<\/p>\n","protected":false},"author":3,"featured_media":15368,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[522],"tags":[2960],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15367"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=15367"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15367\/revisions"}],"predecessor-version":[{"id":15369,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15367\/revisions\/15369"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/15368"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=15367"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=15367"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=15367"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}