{"id":15272,"date":"2017-12-11T16:38:42","date_gmt":"2017-12-11T08:38:42","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=15272"},"modified":"2023-08-18T10:04:20","modified_gmt":"2023-08-18T02:04:20","slug":"%e8%8d%89%e9%85%b8%e5%91%88%e8%89%b2%e5%88%86%e6%9e%90-oxalate-oxalic-acid-colorimetric-assay-kit-biovision%e8%b2%a8%e8%99%9fk663-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=15272","title":{"rendered":"\u8349\u9178\u5448\u8272\u5206\u6790 | Oxalate (Oxalic Acid) Colorimetric Assay Kit Biovision\u8ca8\u865fK663-100 (ABCAM ab196990)"},"content":{"rendered":"

\u8349\u9178\u5206\u6790 <\/strong>| Oxalate (Oxalic Acid) Colorimetric Assay Kit<\/em><\/strong>\u8ca8\u865f<\/strong>K663-100 (Abcam ab196990)<\/a><\/span><\/p>\n

Oxalate (Oxalic Acid) Colorimetric Assay Kit<\/em><\/strong><\/p>\n

\u7522\u54c1<\/strong>\u63cf\u8ff0\uff1a<\/strong>\u8349\u9178\u9e7d\uff08C2O42-\uff09\u4ee5\u8349\u9178\u7684\u5f62\u5f0f\u5b58\u5728\u65bc\u8a31\u591a\u98df\u7269\u548c\u98f2\u6599\uff08\u4f8b\u5982\u83e0\u83dc\uff0c\u8336\u7b49\uff09\u4e2d\u3002 \u5b83\u7a4d\u7d2f\u5728\u8a31\u591a\u690d\u7269\u7d44\u7e54\u4e2d\uff0c\u8d77\u8abf\u7bc0pH\uff0c\u6ef2\u900f\u548c\u9223\u5132\u5b58\u7684\u4f5c\u7528\u3002 \u5728\u52d5\u7269\u4e2d\uff0c\u8349\u9178\u9e7d\u65e2\u53ef\u4ee5\u5f9e\u98f2\u98df\u4e2d\u651d\u53d6\uff0c\u4e5f\u53ef\u4ee5\u5f9e\u809d\u81df\u4e2d\u7684\u4e59\u9187\u9178\u4ee3\u8b1d\u7522\u751f\u3002 \u5728\u6b63\u5e38\u60c5\u6cc1\u4e0b\uff0c\u6bcf\u65e5\u8349\u9178\u8ca0\u8377\u53ef\u4ee5\u901a\u904e\u814e\u81df\u6392\u6cc4\u3002 \u7136\u800c\uff0c\u907a\u50b3\u6027\u7f3a\u9677\u6703\u5c0e\u81f4\u8349\u9178\u9e7d\u6c34\u5e73\u5347\u9ad8\uff0c\u5c0e\u81f4\u9ad8\u8349\u9178\u5c3f\u75c7\uff0c\u4e26\u5c0e\u81f4\u814e\u7d50\u77f3\u7684\u5f62\u6210\u3002 \u56e0\u6b64\uff0c\u8349\u9178\u9e7d\u6c34\u5e73\u7684\u6e2c\u91cf\u5c0d\u65bc\u814e\u7d50\u77f3\u7684\u9810\u9632\uff0c\u8a3a\u65b7\u548c\u76e3\u6e2c\u662f\u6709\u7528\u7684\u3002 BioVision\u7684\u8349\u9178\u9e7d\u5206\u6790\u8a66\u5291\u76d2\u662f\u4e00\u7a2e\u6613\u65bc\u4f7f\u7528\uff0c\u9748\u654f\u5ea6\u9ad8\uff0c\u9069\u61c9\u6027\u5f37\u7684\u8a66\u5291\u76d2\u3002 \u5728\u8a72\u6e2c\u5b9a\u4e2d\uff0c\u8349\u9178\u9e7d\u8207\u8349\u9178\u9e7d\u8f49\u5316\u9ad4\u548c\u8349\u9178\u9e7d\u6df7\u5408\u7269\u53cd\u61c9\u4ee5\u5f62\u6210\u4e2d\u9593\u9ad4\uff0c\u4e2d\u9593\u9ad4\u53c8\u8207\u9ad8\u5ea6\u7279\u7570\u6027\u63a2\u91dd\u53cd\u61c9\u4ee5\u5728450nm\u8655\u7522\u751f\u984f\u8272\u3002 \u5206\u6790\u8a66\u5291\u76d2\u53ef\u6aa2\u6e2c\u4f4e\u65bc20\u03bcM\u7684\u8349\u9178\u9e7d\u6c34\u5e73\u3002<\/p>\n

\"\"<\/p>\n

<\/p>\n

\u529f\u80fd\u7814\u7a76 – \u8349\u9178\u9e7d\u6e2c\u5b9a\u8a66\u5291\u76d2\uff08\u6bd4\u8272\u6cd5\uff09(ab196990)<\/strong><\/span><\/div>\n
8\u5468\u5927\u7684\u5c0f\u9f20\u88ab\u7f6e\u65bc\u7279\u6b8a\u7684\u57fa\u65bc\u86cb\u6e05\u7684\u98f2\u98df\u4e2d10\u9031\uff1aHPC\u98f2\u98df\uff08\u9ad8\u78f7\u9178\u9e7d\u548c\u9223\u98f2\u98df\uff1b20\uff05\u4e73\u9178\uff0c2\uff05\u9223\uff0c1.25\uff05\u78f7\u9178\u9e7d\uff09\uff1bHP\u98f2\u98df\uff08\u9ad8\u78f7\u9178\u9e7d\u98f2\u98df\uff1b0.6%\u9223\uff0c1.20%\u78f7\u9178\u9e7d\uff09\uff1bCO\u98f2\u98df\uff08\u5c0d\u7167\u98f2\u98df\uff1b0.6%\u9223\uff0c0.3%\u78f7\u9178\u9e7d\uff09\uff1bWT\uff1a\u91ce\u751f\u578b\uff1bNpt2a\uff1aNpt2a-\/- \u5c0f\u9f20\u3002\u4f7f\u7528 196990 \u6e2c\u91cf\u8349\u9178\u9e7d\u3002<\/div>\n
<\/div>\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK663-100 (Abcam ab196990)<\/a><\/span><\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (OD 450 nm)<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nMeasurement of Oxalate level in various samples.
\nAnalysis of kidney and liver functions.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple, convenient and high throughput adaptable
\n\u2022 Kit can detect Oxalate levels lower than 20 \u00b5M.<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Oxalate Assay Buffer
\n\u2022 Oxalate Development Buffer
\n\u2022 Oxalate Converter
\n\u2022 Oxalate Enzyme Mix (lyophilized)
\n\u2022 Oxalate Probe (lyophilized)
\n\u2022 Oxalate Standard (lyophilized)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

SAMPLE PREPARATION <\/span><\/strong><\/span><\/p>\n

General Sample information:<\/p>\n

We recommend performing several dilutions of your sample to ensure the readings are within the standard value range.<\/p>\n

We recommend that you use fresh samples. If you cannot perform the assay at the same time, we suggest that you complete the Sample Preparation step before storing the samples. Alternatively, if that is not possible, we suggest that you snap freeze cells or tissue in liquid nitrogen upon extraction and store the samples immediately at -80\u00b0C. When you are ready to test your samples, thaw them on ice. Be aware however that this might affect the stability of your samples and the readings can be lower than expected.<\/p>\n

\u5efa\u8b70\u5c0d\u6a23\u54c1\u9032\u884c\u591a\u6b21\u7a00\u91cb\uff0c\u4ee5\u78ba\u4fdd\u8b80\u6578\u5728\u6a19\u6e96\u503c\u7bc4\u570d\u5167\u3002 \u5efa\u8b70\u4f7f\u7528\u65b0\u9bae\u6a23\u54c1\u3002\u5982\u679c\u60a8\u7121\u6cd5\u540c\u6642\u9032\u884c\u6e2c\u5b9a\uff0c\u5efa\u8b70\u5728\u5b58\u5132\u6a23\u54c1\u4e4b\u524d\u5b8c\u6210\u6a23\u54c1\u88fd\u5099\u6b65\u9a5f\u3002\u6216\u8005\uff0c\u5982\u679c\u4e0d\u53ef\u80fd\u5efa\u8b70\u60a8\u5728\u63d0\u53d6\u5f8c\u5c07\u7d30\u80de\u6216\u7d44\u7e54\u5feb\u901f\u51b7\u51cd\u5728\u6db2\u6c2e\u4e2d\uff0c\u4e26\u7acb\u5373\u5c07\u6a23\u54c1\u5132\u5b58\u5728 -80\u00b0C \u4e0b\u3002\u7576\u60a8\u6e96\u5099\u597d\u6e2c\u8a66\u6a23\u54c1\u6642\uff0c\u5c07\u5176\u5728\u51b0\u4e0a\u89e3\u51cd\u3002\u4f46\u8acb\u6ce8\u610f\uff0c\u9019\u53ef\u80fd\u6703\u5f71\u97ff\u6a23\u54c1\u7684\u7a69\u5b9a\u6027\uff0c\u4e26\u4e14\u8b80\u6578\u53ef\u80fd\u4f4e\u65bc\u9810\u671f\u3002<\/p>\n

Urine sample:<\/p>\n

For urine samples, we recommend collecting samples 24 hours prior to testing. Collect sample (10 mL) in a bottle containing 10 mL of 10 M hydrochloric acid. 11.3.4Centrifuge at 1,000 x g for 10 minutes and either use the supernatant immediately for analysis or save at -20\u00baC for future experiments.<\/p>\n

\u5c3f\u6db2\u6a23\u672c\uff1a<\/p>\n

\u5c0d\u65bc\u5c3f\u6db2\u6a23\u672c\uff0c\u5efa\u8b70\u5728\u6aa2\u6e2c\u524d24\u5c0f\u6642\u63a1\u96c6\u6a23\u672c\u3002\u5c07\u6a23\u54c1 (10 mL) \u6536\u96c6\u5230\u88dd\u6709 10 mL 10 M \u9e7d\u9178\u7684\u74f6\u5b50\u4e2d\u3002 \u4ee5 1,000 x g \u96e2\u5fc3 10 \u5206\u9418\uff0c\u7acb\u5373\u4f7f\u7528\u4e0a\u6e05\u6db2\u9032\u884c\u5206\u6790\u6216\u4fdd\u5b58\u5728 -20\u00b0C \u4e0b\u4ee5\u4f9b\u5c07\u4f86\u5be6\u9a57\u3002<\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u8349\u9178\u5206\u6790 | Oxalate (Oxalic Acid) Colorimetric Assay Kit\u8ca8\u865fK6 […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5380,180],"tags":[3002],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15272"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=15272"}],"version-history":[{"count":6,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15272\/revisions"}],"predecessor-version":[{"id":47312,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15272\/revisions\/47312"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=15272"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=15272"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=15272"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}