{"id":15200,"date":"2017-11-24T11:35:36","date_gmt":"2017-11-24T03:35:36","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=15200"},"modified":"2022-07-21T09:39:17","modified_gmt":"2022-07-21T01:39:17","slug":"%e4%b8%99%e6%b0%a8%e9%85%b8%e6%b0%a8%e5%9f%ba%e8%bd%89%e7%a7%bb%e9%85%b6%ef%bc%88alt%e6%88%96sgpt%ef%bc%89%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-alt-or-sgpt-activity-colorimetricfluorometric","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=15200","title":{"rendered":"\u4e19\u6c28\u9178\u6c28\u57fa\u8f49\u79fb\u9176\uff08ALT\u6216SGPT\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2 | ALT or SGPT Activity Colorimetric\/Fluorometric Assay Kit\u8ca8\u865fK752-100"},"content":{"rendered":"

\u4e19\u6c28\u9178\u6c28\u57fa\u8f49\u79fb\u9176\uff08ALT\u6216SGPT\uff09\u6d3b\u6027\u6bd4\u8272\/<\/strong>\u87a2<\/strong>\u5149\u6e2c\u5b9a\u8a66\u5291\u76d2 <\/strong>| <\/strong>\u8ca8\u865f<\/strong>K752-100<\/a><\/span><\/p>\n

Alanine Aminotransferase (ALT or SGPT) Activity Colorimetric\/Fluorometric Assay Kit<\/em><\/strong><\/p>\n

\"\u00e5\u008e\u009f\u00e7\u0090\u0086\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\"\u4e19\u6c28\u9178\u8f49\u6c28\u9176\uff08ALT\uff09\u662f\u4e00\u7a2e\u8f49\u6c28\u9176\uff08EC 2.6.1.2\uff09\u3002\u5b83\u4e5f\u88ab\u7a31\u70ba\u8840\u6e05\u7a40\u4e19\u8f49\u6c28\u9176\uff08SGPT\uff09\u6216\u4e19\u6c28\u9178\u8f49\u6c28\u9176\uff08ALAT\uff09\u3002 ALT\u5b58\u5728\u65bc\u8840\u6e05\u548c\u5404\u7a2e\u8eab\u9ad4\u7d44\u7e54\u4e2d\uff0c\u4f46\u901a\u5e38\u8207\u809d\u81df\u6709\u95dc\u3002\u5b83\u50ac\u5316\u53cd\u61c9\uff1a\u03b1-\u916e\u620a\u4e8c\u9178+\u4e19\u6c28\u9178\u21cc\u8c37\u6c28\u9178+\u4e19\u916e\u9178\u5b83\u901a\u5e38\u5728\u81e8\u5e8a\u4e0a\u4f5c\u70ba\u8a3a\u65b7\u809d\u529f\u80fd\u6e2c\u8a66\u7684\u4e00\u90e8\u5206\u4f86\u6e2c\u5b9a\u4ee5\u78ba\u5b9a\u809d\u81df\u5065\u5eb7\u3002\u8a3a\u65b7\u4e0a\uff0c\u5e7e\u4e4e\u7e3d\u662f\u4ee5\u55ae\u4f4d\/\u5347\uff08U \/ L\uff09\u4f86\u8861\u91cf\u3002\u5728BioVision\u7684ALT\u6aa2\u6e2c\u8a66\u5291\u76d2\u4e2d\uff0cALT\u50ac\u5316\u6c28\u57fa\u5f9e\u4e19\u6c28\u9178\u5230ket-\u916e\u620a\u4e8c\u9178\u7684\u8f49\u79fb\uff0c\u8a72\u53ef\u9006\u8f49\u6c28\u53cd\u61c9\u7684\u7522\u7269\u662f\u4e19\u916e\u9178\u548c\u8c37\u6c28\u9178\u3002\u5728\u5c07\u5e7e\u4e4e\u7121\u8272\u7684\u63a2\u91dd\u540c\u6642\u8f49\u63db\u6210\u984f\u8272\uff08\u03bbmax= 570nm\uff09\u548c\u87a2\u5149\uff08Ex \/ Em = 535 \/ 587nm\uff09\u7684\u53cd\u61c9\u4e2d\u6aa2\u6e2c\u5230\u4e19\u916e\u9178\u9e7d\u3002\u8a72\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u5feb\u901f\uff0c\u7c21\u55ae\uff0c\u9748\u654f\u548c\u53ef\u9760\u7684\u6aa2\u6e2c\u65b9\u6cd5\uff0c\u9069\u7528\u65bcALT\u7684\u9ad8\u901a\u91cf\u6d3b\u6027\u6aa2\u6e2c\uff0c\u6aa2\u6e2c\u9650\u70ba\u6bcf\u5b5410 mU\u3002<\/p>\n

\"\"<\/p>\n

\"k752\"<\/a><\/p>\n

\"ELISA<\/a><\/p>\n

ELISA<\/u><\/strong>\u5100\u5668\u8a2d\u5099\u8acb\u6309\u6b64<\/u><\/strong><\/a>\u00a0<\/a>\u00a0(\u6a19\u914d405\u3001450\u3001492\u3001630 nm\u6ffe\u7247\/\u6700\u9ad8\u53ef\u64f4\u5145\u81f37\u6ffe\u7247\uff09\uff08k752 \u03bb= 570 nm\uff09<\/span><\/p>\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK752-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (570 nm) or Fluorescence (Ex\/Em 535\/587 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nEvaluation of pentose phosphate pathway<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure
\n\u2022 Fast and convenient
\n\u2022 Sensitive assays for measuring ALT activity in various biological samples and the kit is also suitable for high throughput analyses.<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 ALT Assay Buffer
\n\u2022 OxiRed Probe (in DMSO)
\n\u2022 ALT Enzyme Mix
\n\u2022 ALT Substrate
\n\u2022 Pyruvate Standard (100 nmol\/\u00b5l)
\n\u2022 ALT positive control<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n


\n\"\u76f8\u95dc\u8a66\u5291\"\u00a0\u767d\u86cb\u767d\uff08BCG\uff09\u6aa2\u6e2c<\/a>\u00a0|\u00a0\uff08AST\uff09\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291<\/a>\u00a0|\u00a0ALT\/SGPT\uff09\u6d3b\u6027\u6bd4\u8272\u87a2\u5149\u6e2c\u5b9a\u8a66\u5291<\/a>\u00a0|\u00a0\u00a0Ammonia\u6e2c\u5b9a\u8a66\u5291<\/a>\u00a0|\u00a0<\/span><\/strong>\u81bd\u7d05\u7d20\u5206\u6790\u8a66\u5291\u76d2<\/a>\u00a0<\/strong><\/span><\/p>\n

\u00a0\u5f15\u7528\u6587\u737b<\/strong><\/p>\n

\n
Takasu, C. et al., (2017) Treatment with dimethyl fumarate ameliorates liver ischemia\/reperfusion injury,\u00a0World J Gastroenterol, Oct.2017, 23(25): 4508\u20134516.<\/a><\/div>\n<\/div>\n
\n
Zhang, J. et al., (2017) Jia-Jian-Di-Huang-Yin-Zi decoction reduces apoptosis induced by both mitochondrial and endoplasmic reticulum caspase12 pathways in the mouse model of Parkinson\u2019s disease\u00a0,\u00a0ournal of Ethnopharmacology, May 2017, 203: 69-79<\/a><\/div>\n<\/div>\n
\n
Mohamed Aboul Ezz et al., (2017) The effect of cholesterol loaded cyclodextrins on post-thawing quality of buffalo semen in relation to sperm DNA damage and ultrastructure,\u00a0Reproductive Biology, 2017, 17: 42\u201350<\/a><\/div>\n<\/div>\n
\n
Myungsuk Kim et al., (2017) Chicoric acid attenuate a nonalcoholic steatohepatitis by inhibiting key regulators of lipid metabolism, fibrosis, oxidation, and inflammation in mice with methionine and choline deficiency,\u00a0Mol Nutr Food Res, 2017, 61(5)<\/a><\/div>\n<\/div>\n
\n
Cho, Haaglim et al. (2017) ENOblock, a unique small molecule inhibitor of the non-glycolytic functions of enolase, alleviates the symptoms of type 2 diabetes,\u00a0Sci Rep. 2017 Mar 8;7:44186.<\/a><\/div>\n
<\/div>\n<\/div>\n

\u00a0\"Line\u00e5\u0084\u0080\u00e5\u0099\u00a8\u00e5\u009c\u0096\u00e6\u0096\u008704\"\"Line\u00e5\u0084\u0080\u00e5\u0099\u00a8\u00e5\u009c\u0096\u00e6\u0096\u008701\"\"Line\u00e5\u0084\u0080\u00e5\u0099\u00a8\u00e5\u009c\u0096\u00e6\u0096\u008702\"\"Line\u00e5\u0084\u0080\u00e5\u0099\u00a8\u00e5\u009c\u0096\u00e6\u0096\u008703\"<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u4e19\u6c28\u9178\u6c28\u57fa\u8f49\u79fb\u9176\uff08ALT\u6216SGPT\uff09\u6d3b\u6027\u6bd4\u8272\/\u87a2\u5149\u6e2c\u5b9a\u8a66\u5291\u76d2 | \u8ca8\u865fK752-100 Alanine Ami […]<\/p>\n","protected":false},"author":3,"featured_media":28510,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[183],"tags":[3015],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15200"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=15200"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15200\/revisions"}],"predecessor-version":[{"id":29667,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15200\/revisions\/29667"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/28510"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=15200"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=15200"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=15200"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}