{"id":15196,"date":"2017-11-24T11:25:10","date_gmt":"2017-11-24T03:25:10","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=15196"},"modified":"2024-06-13T18:17:14","modified_gmt":"2024-06-13T10:17:14","slug":"%e5%a4%a9%e5%86%ac%e6%b0%a8%e9%85%b8%e8%bd%89%e6%b0%a8%e9%85%b6%ef%bc%88ast%ef%bc%89%e6%b4%bb%e6%80%a7%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92-astsgot-activity-colorimetric-assay-kit%e8%b2%a8","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=15196","title":{"rendered":"\u5929\u51ac\u6c28\u9178\u8f49\u6c28\u9176\uff08AST\uff09\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2 | AST\/SGOT Activity Colorimetric Assay Kit\u8ca8\u865fK753-100"},"content":{"rendered":"

\u5929\u51ac\u6c28\u9178\u8f49\u6c28\u9176\uff08AST\uff09\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2 <\/strong>| <\/strong>\u8ca8\u865f<\/strong>K753-100<\/a><\/span><\/p>\n

Aspartate Aminotransferase (AST or SGOT) Activity Colorimetric Assay Kit<\/em><\/strong><\/p>\n

\"\u00e5\u008e\u009f\u00e7\u0090\u0086\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\"<\/strong>\u5929\u51ac\u6c28\u9178\u8f49\u6c28\u9176\uff08AST\uff09\uff0c\u4e5f\u88ab\u7a31\u70ba\u8c37\u6c28\u9178 – \u8349\u9170\u4e59\u9178\u8f49\u6c28\u9176\uff08GOT\uff09\u662f\u4e00\u7a2e\u8f49\u6c28\u9176\uff08EC 2.6.1.1\uff09\uff0c\u985e\u4f3c\u65bc\u809d\u81df\u7279\u7570\u6027\u4e19\u6c28\u9178\u8f49\u6c28\u9176\uff08ALT\uff09\u3002\u5118\u7ba1\u81e8\u5e8a\u4e0a\u901a\u5e38\u5c07\u5176\u4f5c\u70ba\u8a3a\u65b7\u6027\u809d\u529f\u80fd\u8a66\u9a57\u7684\u4e00\u90e8\u5206\uff0c\u4f46\u662fAST\u5177\u6709\u66f4\u5ee3\u6cdb\u7684\u81e8\u5e8a\u61c9\u7528\uff0c\u56e0\u70ba\u5b83\u4e5f\u53ef\u80fd\u5728\u5f71\u97ff\u5176\u5b83\u5668\u5b98\u7684\u75be\u75c5\u5982\u5fc3\u808c\u6897\u585e\u7684\u5fc3\u81df\u6216\u808c\u8089\u4e2d\u5347\u9ad8\uff0c\u5728\u6025\u6027\u80f0\u817a\u708e\uff0c\u6025\u6027\u6eb6\u8840\u6027\u8ca7\u8840\uff0c\u56b4\u91cd\u71d2\u50b7\uff0c\u6025\u6027\u814e\u75c5\uff0c\u808c\u8089\u9aa8\u9abc\u75be\u75c5\u548c\u5916\u50b7\u3002\u5b83\u50ac\u5316\u53cd\u61c9\uff1a\u5929\u51ac\u6c28\u9178+ K-\u916e\u620a\u4e8c\u9178\u21cc\u8349\u9170\u4e59\u9178+\u8c37\u6c28\u9178\u5728\u8a3a\u65b7\u4e0a\uff0c\u5b83\u5e7e\u4e4e\u7e3d\u662f\u4ee5\u55ae\u4f4d\/\u5347\uff08U \/ L\uff09\u4f86\u8861\u91cf\u3002\u5728BioVision\u7684AST\u5206\u6790\u8a66\u5291\u76d2\u4e2d\uff0c\u6c28\u57fa\u5f9e\u5929\u51ac\u6c28\u9178\u8f49\u79fb\u5230\u03b2-\u916e\u620a\u4e8c\u9178\u3002\u9019\u7a2e\u53ef\u9006\u8f49\u6c28\u53cd\u61c9\u7684\u7522\u7269\u662f\u8349\u9170\u4e59\u9178\u548c\u8c37\u6c28\u9178\u3002\u5728\u5c07\u5e7e\u4e4e\u7121\u8272\u7684\u63a2\u91dd\u4f34\u96a8\u8f49\u5316\u70ba\u984f\u8272\uff08\u03bbmax= 450nm\uff09\u7684\u53cd\u61c9\u4e2d\u6aa2\u6e2c\u5230\u8c37\u6c28\u9178\u9e7d\u3002\u8a72\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u5feb\u901f\uff0c\u7c21\u55ae\uff0c\u9748\u654f\u548c\u53ef\u9760\u7684\u6aa2\u6e2c\u65b9\u6cd5\uff0c\u9069\u7528\u65bcAST\u7684\u9ad8\u901a\u91cf\u6d3b\u6027\u6aa2\u6e2c\uff0c\u6aa2\u6e2c\u9650\u70ba\u6bcf\u5b5410 mU\u3002<\/p>\n

\"k753\"<\/a><\/p>\n

\"ELISA<\/a><\/p>\n

ELISA<\/u><\/strong>\u5100\u5668\u8a2d\u5099\u8acb\u6309\u6b64<\/u><\/strong><\/a>\u00a0<\/a>\u00a0(\u6a19\u914d405\u3001450\u3001492\u3001630 nm\u6ffe\u7247\/\u6700\u9ad8\u53ef\u64f4\u5145\u81f37\u6ffe\u7247\uff09\uff08k753 \u03bb= 450 nm\uff09<\/span><\/p>\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK753-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (450 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nThis assay detects AST\/ SGOT with a detection limit of 10 mU per well.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure
\n\u2022 Fast, reliable and convenient
\n\u2022 Sensitive assays for measuring AST\/ SGOT activity in various biological samples and the kit is also suitable for high throughput analyses.<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 AST Assay Buffer
\n\u2022 AST Enzyme Mix
\n\u2022 Developer
\n\u2022 AST Substrate
\n\u2022 Glutamate Standard (0.1M)
\n\u2022 AST Positive Control<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n


\n\"\u76f8\u95dc\u8a66\u5291\"\u00a0\u767d\u86cb\u767d\uff08BCG\uff09\u6aa2\u6e2c<\/a>\u00a0|\u00a0\uff08AST\uff09\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291<\/a>\u00a0|\u00a0ALT\/SGPT\uff09\u6d3b\u6027\u6bd4\u8272\u87a2\u5149\u6e2c\u5b9a\u8a66\u5291<\/a>\u00a0|\u00a0\u00a0Ammonia\u6e2c\u5b9a\u8a66\u5291<\/a>\u00a0|\u00a0<\/span><\/strong>\u81bd\u7d05\u7d20\u5206\u6790\u8a66\u5291\u76d2<\/a>\u00a0<\/strong><\/span><\/p>\n

\n
Andre Apostolatos, Preclinical testing of 5-amino-1-((1R,2S,3S,4R)-2,3-dihydroxy-4-methylcyclopentyl)-1H-imidazole-4-carboxamide: a potent protein kinase C-\u03b9 inhibitor as a potential prostate carcinoma therapeutic.\u00a0Anticancer drugs, Jan 2019; 30204596.<\/a><\/div>\n<\/div>\n
\n
Haibao Zhu, Spatial control of\u00a0in vivo\u00a0CRISPR\u2013Cas9 genome editing via nanomagnets.\u00a0Nat Biomed Eng, Nov 2018; \u00a030944431.<\/a><\/div>\n<\/div>\n
\n
Myungsuk Kim et al., (2017) Chicoric acid attenuate a nonalcoholic steatohepatitis by inhibiting key regulators of lipid metabolism, fibrosis, oxidation, and inflammation in mice with methionine and choline deficiency,\u00a0Mol Nutr Food Res, 2017, 61(5)<\/a><\/div>\n<\/div>\n
\n
Mohamed Aboul Ezz et al., (2017) The effect of cholesterol loaded cyclodextrins on post-thawing quality of buffalo semen in relation to sperm DNA damage and ultrastructure,\u00a0Reproductive Biology, 2017, 17: 42\u201350<\/a><\/div>\n<\/div>\n
\n
Mahesh, Malleswarapu et al. (2016) Carrot Juice Administration Decreases Liver Stearoyl-CoA Desaturase 1 and Improves Docosahexaenoic Acid Levels, but Not Steatosis in High Fructose Diet-Fed Weanling Wistar Rats.,\u00a0Prev Nutr Food Sci. 2016 Sep;21(3):171-180.<\/a><\/div>\n
\n

\"smart\"<\/a>\"Spectrumlab<\/a><\/p>\n

\"\u5be6\u9a57\u8a2d\u50992\"<\/a>\"\u00e7\u008e\u00bb\u00e7\u0089\u0087\u00e6\u008e\u0083\u00e6\u008f\u008f\"<\/a><\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

\u5929\u51ac\u6c28\u9178\u8f49\u6c28\u9176\uff08AST\uff09\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2 | \u8ca8\u865fK753-100 Aspartate Aminotransfe […]<\/p>\n","protected":false},"author":3,"featured_media":28510,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[498],"tags":[3016],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15196"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=15196"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15196\/revisions"}],"predecessor-version":[{"id":29664,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15196\/revisions\/29664"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/28510"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=15196"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=15196"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=15196"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}