{"id":15047,"date":"2017-11-06T11:54:50","date_gmt":"2017-11-06T03:54:50","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=15047"},"modified":"2022-07-21T09:52:00","modified_gmt":"2022-07-21T01:52:00","slug":"%e7%84%a6%e7%a3%b7%e9%85%b8%e9%b9%bd%ef%bc%88ppi%ef%bc%89%e5%88%86%e6%9e%90%e8%a9%a6%e5%8a%91%e7%9b%92-pyrophosphate-ppi-assay-kit%e8%b2%a8%e8%99%9fk568-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=15047","title":{"rendered":"\u7126\u78f7\u9178\u9e7d\uff08PPi\uff09\u5206\u6790\u8a66\u5291\u76d2 | Pyrophosphate (PPi) Assay Kit\u8ca8\u865fK568-100"},"content":{"rendered":"

\u7126\u78f7\u9178\u9e7d\uff08PPi\uff09\u5206\u6790\u8a66\u5291\u76d2 | <\/strong>\u8ca8\u865f<\/strong>K568-100<\/a><\/span><\/p>\n

Pyrophosphate (PPi) Assay Kit(Fluorometric\/Colorimetric)<\/em><\/strong><\/p>\n

\"biovision\u00e5\u0095\u0086\u00e5\u0093\u0081\u00e6\u008f\u0092\u00e5\u009c\u0096\"<\/p>\n

\"\u00e5\u008e\u009f\u00e7\u0090\u0086\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\"\u7121\u6a5f\u7126\u78f7\u9178\u9e7d\uff08Inorganic pyrophosphate, diphosphate\u4e8c\u78f7\u9178\u9e7d\u00a0\uff0c\u7126\u78f7\u9178pyrophosphoric acid\u6216PPi\uff09\u662f\u5728\u8a31\u591a\u751f\u5316\u53cd\u61c9\u671f\u9593\u5f62\u6210\u6216\u4f5c\u70ba\u5e95\u7269\u6240\u9700\u7684\u5c0f\u5206\u5b50\u3002\u7522\u751fPPi\u7684\u9176\u4fc3\u53cd\u61c9\u5305\u62ecATP\u6c34\u89e3 (ATP hydrolysis) \uff0cDNA\u548cRNA\u805a\u5408(DNA and RNA polymerization)\uff0c\u74b0AMP\u5f62\u6210 (cyclic AMP formation)\u548c\u8102\u80aa\u9178 (fatty acids) \u7684\u9176\u4fc3\u6d3b\u5316\u4ee5\u5f62\u6210\u5b83\u5011\u7684CoA\u916f (CoA esters)\u3002\u76f8\u53cd\uff0c\u4f7f\u7528PPi\u7684\u9176\u53ef\u4ee5\u5305\u62ec\u74b0\u5316\u9176 (cyclases)\uff0c\u6c34\u89e3\u9176 (\u00a0hydrolases ) \u548c\u9023\u63a5\u9176 (ligases)\u3002\u5728\u9aa8\u4ee3\u8b1d (bone metabolism)\uff0c\u814e\u7d50\u77f3\u75be\u75c5 (\u00a0renal stone disease) \u548c\u67d0\u4e9b\u985e\u578b\u7684\u95dc\u7bc0\u708e (\u00a0arthritis) \u7684\u7814\u7a76\u4e2d\uff0c\u8840\u6f3f (plasma)\uff0c\u8840\u6e05(serum)\u548c\u5176\u4ed6\u751f\u7269\u6db2\u9ad4 (biologic fluids)\u6e2c\u91cf\u7121\u6a5f\u7126\u78f7\u9178\u9e7d\uff08PPi\uff09\u662f\u91cd\u8981\u7684\u3002 BioVision\u7684\u7126\u78f7\u9178\u9e7d\uff08Pyrophosphate (PPi) Assay Kit , K568\uff09\u5206\u6790\u8a66\u5291\u76d2\uff0c\u70ba\u751f\u7269\u6750\u6599\u4e2d\u6e38\u96e2\u7121\u6a5f\u7126\u78f7\u9178\u9e7d\u6c34\u5e73\u7684\u6e2c\u5b9a\u63d0\u4f9b\u4e86\u4e00\u7a2e\u5feb\u901f\uff0c\u65b9\u4fbf\u548c\u8d85\u9748\u654f\u7684\u65b9\u6cd5\u3002\u751f\u7269\u904e\u7a0b\u4e2d\u7522\u751f\u7684PPi\u901a\u904e\u4e00\u7cfb\u5217\u5229\u7528\u5c08\u6709\u9176\u6df7\u5408\u7269\u548c\u63a2\u91dd\u7684\u53cd\u61c9\u9032\u884c\u6aa2\u6e2c\uff0c\u7522\u751f\u7a69\u5b9a\u7684\u7522\u7269\uff0c\u53ef\u4ee5\u901a\u904e\u6bd4\u8272\u6216\u87a2\u5149\u8b80\u6578\u9032\u884c\u5b9a\u91cf\u3002\u7522\u751f\u7684\u87a2\u5149\uff08Ex \/ Em = 535\/587 nm\uff09\u6216\u8005\u984f\u8272\uff08OD\uff1a570 nm\uff09\u5f37\u5ea6\u8207\u7126\u78f7\u9178\u9e7d\u7684\u6fc3\u5ea6\u6210\u6b63\u6bd4\uff0c\u53ef\u4ee5\u9032\u884c\u7cbe\u78ba\u7684\u6e2c\u91cf\u3002\u55ae\u9ad4\u7121\u6a5f\u78f7\u9178\u9e7d\uff08Pi\uff09\u4e0d\u5e72\u64fe\u6e2c\u5b9a\u3002\u6aa2\u6e2c\u65b9\u6cd5\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u55ae\u7684\u65b9\u6cd5\uff0c\u9069\u7528\u65bc\u5404\u7a2e\u751f\u7269\u6a23\u54c1\uff0c\u4e26\u4e14\u53ef\u4ee5\u4ee5\u65b9\u4fbf\u7684\u5fae\u91cf\u6ef4\u5b9a\u677f (\u00a0microtiter-plate format) \u683c\u5f0f\u9032\u884c\u3002\u8a72\u8a66\u5291\u76d2\u5206\u5225\u63d0\u4f9b\u4e86\u8db3\u5920\u7684\u8a66\u5291\uff0c \u53ef\u7528\u65bc100\u500b\u87a2\u5149\u6e2c\u5b9a\u621650\u500b\u6bd4\u8272\u6e2c\u5b9a\u3002\u8a72\u8a66\u5291\u76d2\u53ef\u5728\u8840\u6f3f\u548c\u8840\u6e05\u6a23\u54c1\u4e2d\u6aa2\u6e2c\uff0c\u6700\u4f4e\u9748\u654f\u5ea6\u70ba1.8\u03bcM\u7684PPi\u3002<\/p>\n

\"\"\"\"\"\"<\/p>\n

\"K568\"<\/a><\/p>\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK568-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Kit Summary<\/strong><\/td>\n\u2022 Detection method- Fluorescence (Ex\/Em = 535\/587 nm) or Absorbance (OD 570 nm)
\n\u2022 Species reactivity- Mammalian
\n\u2022 Applications- Estimation of Pyrophosphate in various biological samples.
\n\u2022 Detection limit\/sensitivity: Can detect as little as 2 \u00b5M of Pyrophosphate<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nDetection method- Fluorescence (Ex\/Em = 535\/587 nm) or Absorbance (OD 570 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nEukaryotes<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n\u2022 Determination of PPi concentration in biological samples
\n\u2022 Monitor pyrophosphate release by a variety of enzymes
\n\u2022 Screening inhibition or activities of enzymes that consume pyrophosphate<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple, sensitive and HTP adaptable Protocol
\n\u2022 Specific for Pyrophosphate: other molecules containing phosphate groups do not interference with the assay
\n\u2022 This kit can detect as little as 2 \u00b5M of Pyrophosphate in a variety of biological samples<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 PPi Assay Buffer
\n\u2022 PPi Buffer Supplement
\n\u2022 PPi Substrate
\n\u2022 PPi Enzyme Mix
\n\u2022 PPi Developer
\n\u2022 PPi Probe
\n\u2022 PPi Standard (1 mM)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"smart\"<\/a>\"Spectrumlab<\/a>\"\u5be6\u9a57\u8a2d\u50992\"<\/a>\"\u00e7\u008e\u00bb\u00e7\u0089\u0087\u00e6\u008e\u0083\u00e6\u008f\u008f\"<\/a>\"ELISA<\/a><\/p>\n

 <\/p>\n

\u00a0<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u7126\u78f7\u9178\u9e7d\uff08PPi\uff09\u5206\u6790\u8a66\u5291\u76d2 | \u8ca8\u865fK568-100 Pyrophosphate (PPi) Assay K […]<\/p>\n","protected":false},"author":3,"featured_media":28510,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[3049],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15047"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=15047"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15047\/revisions"}],"predecessor-version":[{"id":29596,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15047\/revisions\/29596"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/28510"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=15047"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=15047"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=15047"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}