{"id":15020,"date":"2017-10-31T16:10:26","date_gmt":"2017-10-31T08:10:26","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=15020"},"modified":"2025-11-12T18:28:37","modified_gmt":"2025-11-12T10:28:37","slug":"%e6%b0%b8%e7%94%9f%e5%8c%96%e6%af%9b%e5%9b%8a%e7%9c%9f%e7%9a%ae%e4%b9%b3%e9%a0%ad%e7%b4%b0%e8%83%9e-sv40-immortalized-hair-follicle-dermal-papilla-cells-sv40-%e8%b2%a8%e8%99%9ft0500","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=15020","title":{"rendered":"\u6c38\u751f\u5316\u6bdb\u56ca\u771f\u76ae\u4e73\u982d\u7d30\u80de – SV40 | Immortalized Hair Follicle Dermal Papilla Cells -SV40 \u8ca8\u865fT0500"},"content":{"rendered":"

HOT!!<\/strong><\/span>\u00a0<\/u>\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c<\/span><\/a>\u00a0|<\/strong>\u00a0DAPI\u67d3\u6838<\/a>\u00a0|<\/strong>\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291<\/a>\u00a0|<\/strong>\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291<\/a>\u00a0|<\/strong>\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49\u7cfb\u5217\u5546\u54c1<\/a><\/span><\/span><\/p>\n

A<\/strong>bmgood<\/strong>\u4e0d<\/strong>\u673d<\/strong>\u5316\u7cfb\u5217\u5546\u54c1<\/strong>\u00a0(immortalized cell)<\/strong><\/span><\/p>\n

\u6c38\u751f\u5316\u6bdb\u56ca\u771f\u76ae\u4e73\u982d\u7d30\u80de – S<\/a>V40\u00a0<\/strong><\/span>(<\/strong>\u8ca8\u865f<\/strong>T0500<\/strong>)\u00a0<\/strong>Immortalized Hair Follicle Dermal Papilla Cells -SV40<\/p>\n

\u00a0<\/strong>\u4eba\u6bdb\u56ca\u771f\u76ae\u4e73\u982d\u7d30\u80de\u662f\u5f9e\u982d\u76ae\u6bdb\u56ca\u7d30\u80de\u5916\u57fa\u8cea\u4e2d\u5206\u96e2\u51fa\u7684\u982d\u9aee\u4e73\u982d\u5206\u96e2\u7684\u9ad8\u6d3b\u6027\u9593\u5145\u8cea\u7d30\u80de\u3002 \u771f\u76ae\u4e73\u982d\u7d30\u80de\u901a\u904e\u6d89\u53ca\u6bdb\u56ca\u7d30\u80de\u7684\u4e0a\u76ae – \u9593\u8cea\u76f8\u4e92\u4f5c\u7528\u5728\u63a7\u5236\u6bdb\u9aee\u751f\u9577\u5468\u671f\u548c\u7522\u751f\u4e2d\u8d77\u91cd\u8981\u4f5c\u7528\u3002 \u5b83\u5011\u7684\u5b58\u6d3b\u7531\u4fe1\u865f\u8f49\u5c0e\u9014\u5f91\u5982ERK\u548cAkt\u8abf\u7bc0\u3002 \u9019\u4e9b\u7d30\u80de\u53ef\u7528\u65bc\u767c\u80b2\u548c\u8a55\u4f30\u6bdb\u9aee\u751f\u9577\u7522\u7269\u4e26\u9451\u5b9a\u6bdb\u56ca\u5167\u7684\u7d30\u80de\u7fa4\u3002 \u6b64\u5916\uff0c\u5b83\u5011\u53ef\u7528\u65bc\u96c4\u6fc0\u7d20\u963b\u65b7\u8a66\u5291\u7684\u9ad4\u5916\u7be9\u9078\uff0c\u56e0\u70ba\u5b83\u5011\u5177\u6709\u96c4\u6fc0\u7d20\u53d7\u9ad4\u3002<\/p>\n

<\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

Immortalized Hair Follicle Dermal Papilla Cells – hTERT\u6bdb\u56ca\u771f\u76ae\u4e73\u982d\u7d30\u80de<\/strong>Adherent \/ Multipolar | \u4e0d\u6747\u65b9\u6cd5: hTERT | \u4f86\u6e90Hair Follicle (Female\u6350\u8d08\u7a2e\u65cf\u767d\u7a2e\u4eba) \u3002\u7d30\u80de\u57f9\u990a\u689d\u4ef6: \u4f7f\u7528PriCoat\u2122T25\u57f9\u990a\u74f6 (\u8ca8\u865fG299<\/a>) \u4ee5\u6700\u4f73\u5316\u521d\u4ee3\u548c\u4e0d\u673d\u7d30\u80de\u7684\u751f\u9577\u3001\u589e\u6b96\u548c\u64f4\u589e\u3002\u6216\u5857\u5c64\u7d30\u80de\u5916\u57fa\u8cea(Applied Cell Extracellular Matrix) \u589e\u5f37\u7d30\u80de\u8cbc\u9644 (\u8ca8\u865fG422<\/a>)\u3002Grow cells in ECM-coated culture vessels with the following conditions. \u57f9\u990a\u6db2PriGrowI II(\u8ca8\u865f\u00a0TM003)\uff0c\u80ce\u725b\u8840\u6e05(Cat. No.\u00a0TM999<\/a>)\u6700\u7d42\u6fc3\u5ea6\u70ba10%\u4e26\u586b\u52a01% Penicillin\/Streptomycin \u30021%. Atmosphere: air, 95%; Carbon dioxide (CO2), 5%. Temperature: 37.0\u00b0C\u7d30\u80de\u7279\u6027<\/strong>:<\/strong>\u00a040-42 hours\u3002\u578b\u614b\u70baMultipolar.\u3002\u5efa\u8b70\u7e7c\u4ee3\u7d30\u80de\u6578\u70ba6,000 – 10,000 cells per cm^2\u3002\u7d30\u80de\u57f9\u990a\u6db2\u66f4\u63db\u5927\u7d042-3\u5929\uff1b\u7d30\u80de\u7e7c\u4ee3\u70ba\u80f0\u86cb\u767d\u9176\u6eb6\u6db20.25% trypsin(TM050) \u7684\u4e0b37\u2103\u4e0bincubator 3-5\u5206\u9418\uff0c\u76f4\u5230\u7d30\u80de\u74b0\u7e5e\uff0c\u5728\u986f\u5fae\u93e1\u4e0b\u4e0d\u6642\u76e3\u6e2c\u3002\u7d30\u80de\u5916\u57fa\u8ceaCoating dish\uff0c\u7528PBS\u7a00\u91cbG422\uff081\uff1a1\uff09\u4e26\u5857\u8986\u66f4\u5c11\u7684\u6642\u9593\u3002\u81a0\u539f\u86cb\u767d\u542b\u91cf\u8f03\u9ad8\uff0c\u5f9e\u800c\u4f7f\u8207\u7d30\u80de\u7684\u7d50\u5408\u66f4\u5f37\u3002\u53ef\u4ee5\u5617\u8a66\u6e1b\u5c11incubator\u6642\u9593\u4ee5\u53ca\u5857\u8986\u66f4\u8584\u7684\u5c64\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat. No.<\/td>\nT0500<\/span><\/td>\n<\/tr>\n
Name<\/td>\nImmortalized Hair Follicle Dermal Papilla Cells – SV40<\/td>\n<\/tr>\n
Description<\/td>\n\n

Human Hair Follicle Dermal Papilla cells are highly active mesenchymal cells isolated from the hair papilla embedded in extracellular matrix of scalp hair follicles. Dermal Papilla Cells play a significant role in controlling the hair growth cycle and production by involving in the epithelial-mesenchymal interaction of hair follicle cells. Their survival is regulated by signal transduction pathways such as ERK and Akt.<\/p>\n

These cells can be used for development and evaluation of hair growth products and identifying cell populations within the hair follicle. In addition, they can be used for\u00a0in vitro<\/em>\u00a0screening of androgen blocking reagents since they have androgen receptors.<\/td>\n<\/tr>\n

Organism<\/td>\nHuman (H. sapiens)<\/td>\n<\/tr>\n
Tissue<\/td>\nHair Follicle<\/td>\n<\/tr>\n
Donor History<\/td>\nMale, 66, Caucasian<\/td>\n<\/tr>\n
Growth Properties<\/td>\nAdherent, multipolar<\/td>\n<\/tr>\n
Cell Type<\/td>\nImmortalized Cells<\/td>\n<\/tr>\n
Storage Condition<\/td>\nVapor phase of liquid nitrogen, or below -130\u00b0C.<\/td>\n<\/tr>\n
Shipping Conditions<\/td>\nShip with dry ice.<\/td>\n<\/tr>\n
Product Format<\/td>\nFrozen<\/td>\n<\/tr>\n
Intended Use<\/td>\nThis product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.<\/td>\n<\/tr>\n
BioSafety<\/td>\nII<\/td>\n<\/tr>\n
Certificate of Analysis<\/td>\nFor batch-specific test results, refer to the applicable certificate of analysis that can be found at www.abmgood.com.<\/td>\n<\/tr>\n
Growth Conditions<\/td>\nUse of PriCoat\u2122 T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow III (TM003) + 10% FBS + 1% Penicillin\/Streptomycin Solution (G255), 37.0\u00b0C, 5% CO\u2082<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
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\"\"<\/div>\n

HOT!!<\/strong><\/span>\u00a0<\/u><\/a><\/span>\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c<\/a>\u00a0|<\/strong>\u00a0DAPI\u67d3\u6838<\/a>\u00a0|<\/strong>\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291<\/a>\u00a0|<\/strong>\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291<\/a>\u00a0|<\/strong>\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49\u7cfb\u5217\u5546\u54c1<\/a><\/span><\/p>\n

\"abm\u00e5\u0095\u0086\u00e5\u0093\u0081\u00e6\u008f\u0092\u00e5\u009c\u0096\"<\/p>\n

Abmgood\u4e0d\u673d\u5316\u7cfb\u5217\u5546\u54c1\u00a0(immortalized reagenet)\u00a0Ready-to-use<\/span><\/strong><\/span><\/p>\n

Abmgood\u516c\u53f8\u65bc\u4eba\u985e\u8207\u8001\u9f20\u4e0d\u673d\u4e4b\u7d30\u80de\u682a\u4e0a\uff0c\u5177\u6709\u5e7e\u5341\u5e74\u7684\u5be6\u9a57\u7d93\u9a57\uff1b\u63d0\u4f9b\u4e00\u7cfb\u5217\u4e0d\u673d (immortalized)\u5316\u4e4b\u7d30\u80de\u682a\u3001\u53ca\u4e0d\u673d\u5316\u4e4b\u8a66\u5291\u5546\u54c1\u3002ready-to-use\u5b9a\u91cf\u597d\u4e4bretrovirus\u3001lentiviru\u3001 adenovirus\uff0c\u53ef\u76f4\u63a5\u52a0\u65bc\u7d30\u80de\u4e2d\u9032\u884c\u611f\u67d3\u8868\u73fe\uff1b\u75c5\u6bd2\u6db2\u5305\u62ec\u6709SV40\u91cd\u7d44\u75c5\u6bd2\u3001EBV\u91cd\u7d44\u75c5\u6bd2\u3001hTERT\u75c5\u6bd2\u6db2\u3001Myc\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001p53\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001Rb\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001Ras\u57fa\u56e0\u75c5\u6bd2\u6db2\u3002\u63d0\u4f9b106\u53ca109\u5169\u7a2e\u5305\u88dd\u529b\u50f9\u4e4b\u75c5\u6bd2\u6db2\uff1b\u4ea6\u63d0\u4f9b\u4e00\u7cfb\u5217\u75c5\u6bd2\u529b\u50f9\u6fc3\u7e2e\u8207\u5b9a\u91cf\u8a66\u5291\u5546\u54c1\u3002<\/p>\n

\u7d30\u80de\u4e0d\u673d\u5316\u7684\u5de5\u5177\u4e2d\uff0cSV40 T antigen\u662f\u6700\u7c21\u55ae\u4e14\u6700\u5e38\u7528\u4f86\u9020\u6210\u4e0d\u673d\u5316\u7d30\u80de\u751f\u6210\u7684\u65b9\u6cd5\u3002\u591a\u6578\u7684\u75c5\u6bd2\u57fa\u56e0\u548c\u6291\u764c\u57fa\u56e0\uff08p53\u3001Rb\u7b49\uff09\u7684\u4e0d\u6d3b\u5316\u76f8\u95dc\uff0c\u6703\u8a98\u5c0e\u7d30\u80de\u8655\u65bcreplicative senescence\u7684\u968e\u6bb5\u3002\u76ee\u524d\u4e5f\u6709\u7814\u7a76\u986f\u793a\u88ab\u611f\u67d3\u7684\u7d30\u80de\uff0cSV40 T anbtigen\u6703\u8a98\u5c0e\u7aef\u7c92\u9176\uff08Telomerase\uff09\u7684\u6d3b\u6027\u3002\u8207\u7aef\u7c92\uff08telomere\uff09\u9577\u5ea6\u76f8\u95dc\u7684Telomerase Reverse Transcriptase protein (TERT)\u662f\u4e00\u7a2e\u4e0d\u673d\u7d30\u80de\u7684\u7814\u7a76\u61c9\u7528\u3002\u5e73\u5e38\u7d30\u80de\u4e2d\uff0chTERT\u662f\u8655\u65bc\u4e0d\u6d3b\u5316\u614b\uff0c\u5fc5\u8981\u6642\u6703\u9032\u884c\u6d3b\u5316\u4fdd\u6301telomere\u7684\u9577\u5ea6\uff0c\u907f\u514d\u7d30\u80de\u767c\u751freplicative senescence\u7684\u73fe\u8c61\u3002\u67d0\u4e9b\u7d30\u80dehTERT \u904e\u5ea6\u8868\u73fe\uff08over-expression\uff09\u5c0d\u65bc\u8a98\u767c\u7d30\u80de\u4e0d\u673d\u5316\u662f\u7121\u6548\u7684\uff08\u5982\u521d\u4ee3\u4e0a\u76ae\u7d30\u80de\uff09\uff0c\u53cd\u800c\u6703\u9020\u6210\u7d30\u80de\u6b7b\u4ea1\uff1b\u540c\u6642\u8868\u73fehTERT catalytic subunit\u4ee5\u53cap53\u6216RB siRNA\u5728\u4eba\u985e\u521d\u4ee3\u5375\u5de2\u4e0a\u76ae\u7d30\u80de\u6703\u9020\u6210\u7d30\u80de\u4e0d\u673d\u5316\u3002\u904e\u5ea6\u8868\u73feRas\u6216Myc T58A mutants\u4e5f\u6709\u521d\u4ee3\u7d30\u80de\u4e0d\u673d\u5316\u7684\u73fe\u8c61\u7522\u751f\u3002<\/p>\n

A<\/strong>bmgood<\/strong>\u4e0d<\/strong>\u673d<\/strong>\u5316\u7cfb\u5217\u5546\u54c1<\/strong>\u00a0(immortalized cell)<\/strong><\/span><\/p>\n

\u6839\u64da\u7d30\u80de\u985e\u578b\uff0cPrimary cells\u521d\u4ee3\u7d30\u80de\u5728\u9ad4\u5916\u5177\u6709\u5c0f\u65bc10-50\u6b21\u50b3\u4ee3\u7684\u6709\u9650\u58fd\u547d\u3002 \u4e0d\u673d\u5316\u7d30\u80de\u682a\u80fd\u5920\u5ef6\u9577\u589e\u6b96\uff0c\u4e26\u4e14\u5177\u6709\u8207\u89aa\u4ee3\u7d44\u7e54\u76f8\u4f3c\u6216\u76f8\u540c\u7684\u57fa\u56e0\u578b\u548c\u8868\u73fe\u578b\u3002<\/p>\n

\"cell-biology-tab-final<\/p>\n

\u54fa\u4e73\u52d5\u7269\u7d30\u80de\u7684<\/strong>\u4e0d<\/strong>\u673d<\/strong>\u5316Immortalized\u7b56\u7565<\/strong><\/span><\/p>\n

\u5b58\u5728\u7528\u65bc\u5728\u57f9\u990a\u7269\u4e2d\u4e0d<\/strong>\u673d<\/strong>\u5316<\/strong>\u54fa\u4e73\u52d5\u7269\u7d30\u80de\u7684\u5e7e\u7a2e\u65b9\u6cd5\u3002 Abmgood\u591a\u5e74\u7814\u767c\u7d93\u9a57\uff0c\u958b\u767c\u51fa\u4e86\u4e00\u7cfb\u5217Immortalized\u5546\u54c1\uff1b\u5305\u62ec\u5373\u7528\u578b\u9006\u8f49\u9304\u75c5\u6bd2\uff0c\u6162\u75c5\u6bd2\u548c\u817a\u75c5\u6bd2\u8f09\u9ad4\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Gene Overexpressed<\/strong><\/td>\nViruses Offered<\/strong><\/td>\nApplication<\/strong><\/td>\n<\/tr>\n
hTERT<\/a><\/td>\nAdenovirus,
\nLentivirus,
\nRetrovirus<\/td>\n		\u53ef\u9760\u548c\u5e38\u7528\u3002 \u53ef\u80fd\u5728\u4e00\u4e9b\u7d30\u80de\u985e\u578b\uff08\u4f8b\u5982\u4e0a\u76ae\u7d30\u80de\uff09\u4e2d\u5f15\u8d77\u7d30\u80de\u6b7b\u4ea1<\/td>\n<\/tr>\n
SV40 T Antigen<\/a><\/td>\nAdenovirus,
\nLentivirus,
\nRetrovirus<\/td>\n		\u53ef\u9760\u548c\u5e38\u7528\u3002 \u901a\u5e38\u7528\u65bc\u4e0d\u673d\u5316\u4e0a\u76ae\u7d30\u80de\u3002<\/td>\n<\/tr>\n
EBV genes<\/a><\/td>\nEpstein-Barr Virus<\/td>\n\u4e0d\u673d\u5316B\u6dcb\u5df4\u7d30\u80de\uff0c\u6709\u6642\u662fT\u6dcb\u5df4\u7d30\u80de\u3002<\/td>\n<\/tr>\n
HPV E6-E7<\/a><\/td>\nLentivirus<\/td>\n\u4e0d\u673d\u5316\u89d2\u5316\u7d30\u80de\u3002<\/td>\n<\/tr>\n
HOXB8, HOXA9, HOXA10<\/a><\/td>\nLentivirus<\/td>\n\u4e0d\u673d\u5316\u9020\u8840\u7d30\u80de\uff0c\u5305\u62ec\u5de8\u566c\u7d30\u80de\uff0c\u9020\u8840\u7956\u7d30\u80de\u548c\u9aa8\u9ad3\u7956\u7d30\u80de\u3002<\/td>\n<\/tr>\n
Adenoviral E1A-E1B<\/a><\/td>\nLentivirus<\/td>\n\u4e0d\u673d\u5316\u5927\u7bc4\u570d\u7684\u521d\u7d1a\u56d3\u9f52\u52d5\u7269\u7d30\u80de\uff0c\u5305\u62ec\u7f8a\u6c34\u7d30\u80de\uff0c\u539f\u767c\u6027\u4e0a\u76ae\u7d30\u80de\uff0c\u6210\u7e96\u7dad\u7d30\u80de\u548c\u539f\u4ee3\u814e\u7d30\u80de\u3002<\/td>\n<\/tr>\n
p53, Rb siRNA<\/a><\/td>\nLentivirus<\/td>\n\u8207hTERT\u5171\u8868\u9054\u4ee5\u66f4\u597d\u5730\u4f7f\u4e0a\u76ae\u7d30\u80de\u4e0d\u673d\u5316\u3002<\/td>\n<\/tr>\n
Bmi1<\/a><\/td>\nLentivirus<\/td>\n\u4e0d\u673d\u5316\u4e0a\u76ae\u7d30\u80de\u3002<\/td>\n<\/tr>\n
CDK4<\/a><\/td>\nLentivirus<\/td>\n\u8207hTERT\u5171\u540c\u8868\u9054\u66f4\u771f\u5be6\u7684\u7d30\u80de\u6a21\u578b\u3002<\/td>\n<\/tr>\n
Myc T58A (c-Myc), Ras V12 (H-Ras)<\/a><\/td>\nLentivirus<\/td>\n\u4e0d\u673d\u5316\u5404\u7a2e\u7d30\u80de\uff0c\u5305\u62ec\u795e\u7d93\u5e79\u7d30\u80de\uff0c\u521d\u4ee3\u814e\u7d30\u80de\uff0c\u6210\u7d05\u7d30\u80de\u548c\u5404\u7a2e\u4e0a\u76ae\u7d30\u80de\uff08\u539f\u767c\u6027\u524d\u5217\u817a\uff0c\u4e73\u817a\uff0c\u6c23\u9053\u7b49\uff09\u3002<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

Thawing<\/strong>\u7d30\u80de\u89e3\u51cd\u65b9\u6cd5\uff1a(\u6bcf\u7a2e\u7d30\u80de\u5efa\u8b70\u689d\u4ef6\u53ef\u80fd\u6709\u8a31\u4e0d\u540c\uff0c\u4f9d\u7167\u539f\u5ee0\u5efa\u8b70\u64cd\u4f5c\uff0c\u4ee5\u4e0b\u70ba\u901a\u7528\u65b9\u5f0f)\u00a0<\/strong>\u64cd\u4f5c\u65b9\u5f0f\u4e0b\u8f09<\/strong><\/a><\/span><\/span><\/p>\n

1.\u572837\u2103\u6c34\u6d74\u4e2d\u89e3\u51cd\u5c0f\u74f6\uff0c\u76f4\u5230\u53ea\u6709\u4e00\u5c0f\u584a\u51b0\u584a\u3002(DMSO\u5c0d\u7d30\u80de\u6709\u6bd2\u6027\uff0cover-thaw\u904e\u5ea6\u89e3\u51cd\u4e0b\u53ef\u80fd\u9020Stress)<\/span><\/p>\n

2.\u5c07\u7d30\u80de\u8f49\u79fb\u5230\u542b\u67095ml pre-warmed complete media \u4e4b15ml\u7ba1\u4e2d\u3002<\/p>\n

3.\u5c07\u7d30\u80de\u4ee5290xg\u96e2\u5fc35\u5206\u9418\u3002<\/p>\n

4.\u5c0f\u5fc3\u4e1f\u68c4\u4e0a\u6e05\u6db2\uff0c\u4e0d\u8981\u6253\u64fe\u7d30\u80de\u6c89\u6fb1\uff0c\u8f15\u8f15\u5730\u4e0a\u4e0b\u79fb\u6db2\uff0c\u5c07\u7d30\u80de\u91cd\u65b0\u61f8\u6d6e\u57281ml\u5b8c\u5168\u57f9\u990a\u57fa\u4e2d\u3002<\/p>\n

5.\u5c07\u7d30\u80de\u57f9\u990a\u572850,000 – 100,000 cells\/cm2<\/sup>\u3002\u00a0cells will have viability of around 40-50%. Post thawing, it will need 4-5 days for cells to recover in culture; Recommended split ratio is 1:4 to 1:5<\/p>\n

\"\"<\/p>\n

DMSO\u5728\u7d30\u80de\u51cd\u5b58\u4e2d\u7684\u61c9\u7528<\/span><\/strong><\/p>\n

\u4e8c\u7532\u57fa\u4e9e\u78b8\u662f\u4e00\u7a2e\u91cd\u8981\u7684\u6ef2\u900f\u578b\u7d30\u80de\u4fdd\u8b77\u5291\u3002\u5728\u6df1\u4f4e\u6eab\uff08\u96f6\u4e0b200\u5ea6\uff09\u4fdd\u5b58\u7d30\u80de\u6642\u51cd\u5b58\u904e\u7a0b\u4e2d\uff0c\u70ba\u9632\u6b62\u7d30\u80de\u5167\u6db2\u51b0\u6676\u5f62\u6210\u3001\u6ef2\u900f\u58d3\u6539\u8b8a\u3001\u7d30\u80de\u7d50\u69cb\u7d0a\u4e82\u7b49\u5c0e\u81f4\u7684\u640d\u50b7\uff0c\u6709\u5fc5\u8981\u4f7f\u7528\u542b\u6709DMSO\u51b7\u51cd\u4fdd\u8b77\u5291\u3002DMSO\u80fd\u5920\u5feb\u901f\u7a7f\u900f\u7d30\u80de\u819c\u9032\u5165\u7d30\u80de\u4e2d\uff0c\u964d\u4f4e\u51b0\u9ede\u3001\u5ef6\u7de9\u51cd\u5b58\u904e\u7a0b\uff0c\u540c\u6642\u63d0\u9ad8\u7d30\u80de\u5167\u96e2\u5b50\u6fc3\u5ea6\uff0c\u6e1b\u5c11\u7d30\u80de\u5167\u51b0\u6676\u7684\u5f62\u6210\uff0c\u5f9e\u800c\u6e1b\u5c11\u7d30\u80de\u640d\u50b7\u3002\u6df1\u4f4e\u6eab\u6642\u4e8c\u7532\u57fa\u4e9e\u78b8\u7684\u7d30\u80de\u6bd2\u6027\u53d7\u5230\u6291\u5236\u3002\u5fa9\u8607\u6642\u52d5\u4f5c\u8981\u5feb\uff0c\u5118\u5feb\u6d17\u6389\u4e8c\u7532\u57fa\u4e9e\u78b8\uff0c\u5426\u5247\u6703\u9020\u6210\u5c0d\u7d30\u80de\u56b4\u91cd\u7684\u6bd2\u6027\u3002\u4e8c\u7532\u57fa\u4e9e\u78b8\uff08DMSO\uff09\u662f\u76ee\u524d\u6700\u597d\u7684\u7d30\u80de\u51cd\u5b58\u4fdd\u8b77\u5291\uff0c\u4f46\u4e5f\u662f\u4e00\u7a2e\u4ee5\u7d30\u80de\u6bd2\u6027\u5f88\u5927\u7684\u5316\u5b78\u8a66\u9a57\u5291\u3002\u7814\u7a76\u7d50\u679c\u8868\u660e\uff0c\u57f9\u990a\u6db2\u4e2dDMSO\u6fc3\u5ea6\u70ba10%\u6642\uff0c\u7d30\u80de\u751f\u9577\u6291\u5236\u7387\u8fd1100%\uff1b1\u2030\u6fc3\u5ea6\u6642\u6291\u5236\u7387\u70ba35%\uff0c\u5373\u4f7f\u662f0.04\u2030\u7684\u6fc3\u5ea6\uff0cDMSO\u5c0d\u7d30\u80de\u7684\u751f\u9577\u4e5f\u6709\u4e0d\u5229\u7684\u5f71\u97ff\u3002<\/p>\n

Subculture<\/strong>\u7d30\u80de\u7e7c\u4ee3\u65b9\u6cd5:<\/strong>\u00a0(<\/strong>\u6bcf\u7a2e\u7d30\u80de\u5efa\u8b70\u689d\u4ef6\u53ef\u80fd\u6709\u8a31\u4e0d\u540c\uff0c\u4f9d\u7167\u539f\u5ee0\u5efa\u8b70\u64cd\u4f5c\uff0c\u4ee5\u4e0b\u70ba\u901a\u7528\u65b9\u5f0f)<\/strong>\u00a0\u64cd\u4f5c\u65b9\u5f0f\u4e0b\u8f09<\/strong><\/a><\/span><\/span><\/p>\n

1.\u7576\u7d30\u80de70-90\uff05 confluency\u6642\uff0c\u5efa\u8b70\u7e7c\u4ee3\u57f9\u990a\u3002<\/p>\n

2. Aspirate old media\u3002\u61f8\u6d6e\u6db2\u4e2d\u7684\u4e00\u4e9b\u7d30\u80de\u4ecd\u7136\u662f\u6d3b\u7d30\u80de\u3002\u6216\u8005\uff0c\u4e5f\u53ef\u4ee5\u6536\u96c6\u542b\u6709\u61f8\u6d6e\u7d30\u80de\u7684\u4e0a\u6e05\u6db2\u4e26\u96e2\u5fc3\uff08Skip to Step 6 in protocol\uff09\u3002<\/p>\n

3.\u52a0\u51651\uff1a1\u6bd4\u4f8b\u76841X\u7121\u83ccPBS\u548c0.25\uff05\u80f0\u86cb\u767d\u9176-EDTA\uff08TM051\uff09\u3002<\/p>\n

4.\u5728\u5ba4\u6eab\u4e0b\u5b75\u80b2\u7d30\u80de3-5\u5206\u9418\u4e26\u652a\u62cc\u57f9\u990a\u5bb9\u5668\u76f4\u81f390\uff05\u7684\u7d30\u80de\u812b\u843d\u3002<\/p>\n

5. \u4e2d\u548c\u80f0\u86cb\u767d\u9176:\u6dfb\u52a0\u7b49\u91cf\u4e4btrypsin + PBS\u3002<\/p>\n

6.\u6536\u96c6\u7d30\u80de\u4e26\u4ee5290xg\u96e2\u5fc35\u5206\u9418\u3002<\/p>\n

7.\u4e1f\u68c4\u4e0a\u6e05\u6db2\uff0c\u8f15\u8f15\u5730\u4e0a\u4e0b\u79fb\u6db2\uff0c\u5c07\u7d30\u80de\u91cd\u65b0\u61f8\u6d6e\u5728\u5b8c\u5168\u57f9\u990a\u57fa\u4e2d\u3002<\/p>\n

8.\u5efa\u8b70split ratio\u6bd4\u4e0d\u8d85\u904e1\uff1a3\u3002<\/p>\n

Freezing<\/strong>\u7d30\u80de\u51cd\u7d50\uff1a(\u6bcf\u7a2e\u7d30\u80de\u5efa\u8b70\u689d\u4ef6\u53ef\u80fd\u6709\u8a31\u4e0d\u540c\uff0c\u4f9d\u7167\u539f\u5ee0\u5efa\u8b70\u64cd\u4f5c\uff0c\u4ee5\u4e0b\u70ba\u901a\u7528\u65b9\u5f0f)\u00a0<\/strong>\u64cd\u4f5c\u65b9\u5f0f\u4e0b\u8f09<\/a><\/strong><\/span><\/span><\/p>\n

Complete growth medium with decomplemented FBS to a final concentration of 50% from PAN Biotech (P40-37500) or from Nucleus Biologics (FBS1824-001) and 5% DMSO.<\/p>\n

\u5132\u5b58\u6eab\u5ea6\uff1a\u6db2\u6c2e\u6c23\u76f8\u3002\u91cd\u8981\u63d0\u793a\uff1a\u6b64\u7d30\u80de\u4fc2\u5c0dFBS\u6279\u6b21\u975e\u5e38\u654f\u611f\u3002\u6211\u5011\u5f37\u70c8\u5efa\u8b70\u6700\u7d42\u7528\u6236\u4f7f\u7528\u5f9ePAN biotech\u6216Nucleus Biologics \u4e4bFBS\u3002<\/p>\n

\u6162\u75c5\u6bd2\u5305\u88dd\u7cfb\u5217\u7522\u54c1Lentivirus<\/u>\u6162\u75c5\u6bd2\u4f4e\u901f\u7d14\u5316<\/u>(\u7d14\u5316)\u00a0<\/a>|\u00a0Lentivirus\u00a0<\/u>\u6162\u75c5\u6bd2\u9ad8\u6fc3\u5ea6\u7d14\u5316<\/u>(\u7d14\u5316)\u00a0<\/a>|\u00a0Lentivirus<\/u>\u75c5\u6bd2RT-QPCR<\/u>\u5b9a\u91cf<\/u>(\u5b9a\u91cf)<\/a>\u00a0<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

HOT!!\u00a0\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c\u00a0|\u00a0DAPI\u67d3\u6838\u00a0|\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291\u00a0|\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291\u00a0|\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49 […]<\/p>\n","protected":false},"author":3,"featured_media":11653,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5373,453],"tags":[3056],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15020"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=15020"}],"version-history":[{"count":10,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15020\/revisions"}],"predecessor-version":[{"id":53495,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15020\/revisions\/53495"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/11653"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=15020"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=15020"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=15020"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}