{"id":14969,"date":"2017-10-26T11:37:31","date_gmt":"2017-10-26T03:37:31","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=14969"},"modified":"2022-07-21T10:13:19","modified_gmt":"2022-07-21T02:13:19","slug":"il17rb-sirna-oligos-set-human-%e5%9f%ba%e5%9b%a0%e6%b2%89%e9%bb%98-sirna%e5%af%a1%e6%a0%b8%e8%8b%b7%e9%85%b8%e9%9b%99%e9%8f%88%e9%ab%94%e8%b2%a8%e8%99%9f-i510917","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=14969","title":{"rendered":"IL17RB siRNA Oligos set (Human) | \u57fa\u56e0\u6c89\u9ed8-siRNA\u5be1\u6838\u82f7\u9178\u96d9\u93c8\u9ad4\u8ca8\u865f i510917"},"content":{"rendered":"

IL17RB siRNA Oligos set (Human)<\/strong> | <\/strong>\u8ca8\u865f<\/strong>\u00a0\u00a0 i510917<\/strong><\/a><\/span><\/p>\n

siRNA\u662f\u7528\u65bc\u57fa\u56e0\u6c89\u9ed8\u7684\u5f37\u5927\u5de5\u5177\uff0c\u53ef\u4ee5\u901a\u904e\u591a\u7a2e\u65b9\u6cd5\u905e\u9001\u81f3\u9776\u7d30\u80de\u6216\u5bbf\u4e3b\uff0c\u5305\u62ec\u817a\u75c5\u6bd2\uff0c\u6162\u75c5\u6bd2\u6216\u76f4\u63a5\u8f49\u67d3DNA\u8cea\u7c92\u6216\u96d9\u93c8RNA\uff08dsRNA\uff09\u3002 siRNA oligo\u96d9\u93c8\u9ad4\u662f\u5feb\u901f\u548c\u5bb9\u6613\u4f7f\u7528\u7684\uff0c\u4e26\u4e14\u53ef\u4ee5\u5728\u9ad4\u5916\u548c\u9ad4\u5167\u7528\u65bc\u5feb\u901f\u548c\u77ed\u66ab\u7684\u57fa\u56e0\u6c89\u9ed8\u800c\u4e0d\u6539\u8b8a\u5bbf\u4e3b\u57fa\u56e0\u7d44\u3002For stable knockdown via lentivirus, please see our lentiviral siRNA library products.abm\u63d0\u4f9b\u4e86\u91dd\u5c0d\u4efb\u4f55\u4eba\uff0c\u5c0f\u9f20\u6216\u5927\u9f20\u611f\u8208\u8da3\u7684\u57fa\u56e0\u7684\u9810\u5148\u8a2d\u8a08\u76843\u7a2esiRNA\u5be1\u6838\u82f7\u9178\u96d9\u93c8\u9ad4\uff0c\u4ee5\u53ca\u5ba2\u6236\u5be6\u9a57\u7684\u967d\u6027\u548c\u9670\u6027\u5c0d\u7167\u3002\u96d9\u93c8\u9ad4\u53ef\u4ee5\u55ae\u7368\u8f49\u67d3\u6216\u5408\u4f75\u5728\u4e00\u8d77\u4ee5\u5be6\u73fe\u76ee\u6a19\u57fa\u56e0\u7684knockdown\uff0c\u6700\u5e38\u898b\u7684\u662f\u901a\u904eqPCR\u6216western\u5370\u8de1\u9032\u884c\u8a55\u4f30\u3002\u63d0\u4f9b\u4e4bsiRNA\u5be1\u6838\u82f7\u9178\u662f\u5316\u5b78\u4fee\u98fe\u7684\uff082′-OMe\uff09\uff0c\u7121\u9700\u589e\u52a0\u984d\u5916\u7684\u96fb\u8377\uff0c\u5f9e\u800c\u63d0\u9ad8\u9ad4\u5916\u548c\u9ad4\u5167\u7684\u7a69\u5b9a\u6027\u548c\u589e\u5f37\u7684knockdown\u4f5c\u7528\uff0c\u9810pre-annealed,\u53ca lyophilized powder\u51cd\u4e7e\u7c89\u672b\u904b\u8f38\uff0c\u4ee5\u4fbf\u66f4\u9577\u6642\u9593\u548c\u66f4\u7a69\u5b9a\u7684\u5132\u5b58\u3002<\/p>\n

\"\"<\/p>\n

SiRNA Oligos\u7522\u54c1\u7279\u9ede<\/strong><\/p>\n

    \n
  1. HPLC\u7d14\u5316\uff1asiRNA\u542b\u91cf> 97\uff05<\/li>\n
  2. \u8a2d\u7f6e3\u500bsiRNA\u5be1\u6838\u82f7\u9178\u96d9\u93c8\u9ad4\uff0c\u6700\u5927\u9650\u5ea6\u5730maximum knockdown<\/li>\n
  3. \u6bcf\u500bsiRNA\u5be1\u6838\u82f7\u9178\u96d9\u93c8\u9ad4\u70ba19-23\u500b\u9e7c\u57fa<\/li>\n
  4. \u5316\u5b78\u4fee\u98fe\uff082′-OMe\uff09\u7528\u65bc\u589e\u52a0\u9ad4\u5916\u548c\u9ad4\u5167\u7684\u7a69\u5b9a\u6027\uff0c\u5177\u66f4\u9ad8\u7684\u529f\u6548\u548c\u66f4\u4f4e\u7684\u6bd2\u6027<\/li>\n
  5. \u9644\u6b63\u9762\u548c\u8ca0\u9762\u5c0d\u7167<\/li>\n
  6. \u514d\u8cbb\u8a2d\u8a08; \u4fdd\u8b49knockdown\u81f3\u5c1170\uff05<\/li>\n<\/ol>\n

    \"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
    Species<\/strong><\/td>\nHuman<\/td>\n<\/tr>\n
    Accession Number<\/strong><\/td>\nNM_018725<\/a><\/td>\n<\/tr>\n
    Components Included<\/strong><\/td>\n3 x 5nmol (2 OD duplex, 1 OD single) of chemically modified siRNA oligos (3 target sequences)
    \n1 x 2.5nmol (1 OD duplex, 0.5 OD single) of positive control siRNA
    \n1 x 2.5nmol (1 OD duplex, 0.5 OD single) of negative control siRNA
    \n1 x 2.5nmol (1 OD duplex, 0.5 OD single) of FAM-labeled negative control siRNA<\/td>\n<\/tr>\n
    Conversion of ODs, nmols and \u03bcg<\/strong><\/td>\n1 OD = 2.5 nmol siRNA (duplex) = 5 nmol siRNA (single-stranded) = 33 \u03bcg<\/td>\n<\/tr>\n
    Additional Reagents Required (not supplied)<\/strong><\/td>\nRNAifectin(Cat.No.G073)<\/a><\/td>\n<\/tr>\n
    Target Sequence<\/strong><\/td>\nSequence available upon placing order<\/td>\n<\/tr>\n
    Chemical Modifiation<\/strong><\/td>\n2′-OMe modification for increased stability and low cell toxicity<\/td>\n<\/tr>\n
    Format<\/strong><\/td>\nLyophilized double stranded siRNA oligos<\/td>\n<\/tr>\n
    Purification<\/strong><\/td>\nHPLC<\/td>\n<\/tr>\n
    Storage<\/strong><\/td>\nUpon receipt, -70\u00b0C for long term storage; -20\u00b0C for short term storage.
    \nRecommended storage concentration is above 20\u03bcM.
    \nAfter reconstitution, store in aliquots to avoid repeated freeze-thawing.<\/td>\n<\/tr>\n
    Shelf Life<\/strong><\/td>\nUp to 6 months if stored at -20\u00b0C or below after reconstitution in a non-frost free freezer.
    \n1 year lyophilized (when at -20\u00b0C or below in a non-frost free freezer)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

    \u00a0<\/strong><\/p>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    IL17RB siRNA Oligos set (Human) | \u8ca8\u865f\u00a0\u00a0 i510917 siRNA\u662f\u7528\u65bc […]<\/p>\n","protected":false},"author":3,"featured_media":14970,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[3069],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14969"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=14969"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14969\/revisions"}],"predecessor-version":[{"id":14975,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14969\/revisions\/14975"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/14970"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=14969"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=14969"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=14969"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}