{"id":14913,"date":"2017-10-22T13:30:25","date_gmt":"2017-10-22T05:30:25","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=14913"},"modified":"2022-07-21T10:14:08","modified_gmt":"2022-07-21T02:14:08","slug":"hdac6%e6%b4%bb%e6%80%a7%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92%ef%bc%88%e8%9e%a2%e5%85%89%e6%b8%ac%e5%ae%9a%ef%bc%89-%e8%b2%a8%e8%99%9fk466-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=14913","title":{"rendered":"HDAC6\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\uff08\u87a2\u5149\u6e2c\u5b9a\uff09 | HDAC6 Activity Assay Kit \u8ca8\u865fK466-100"},"content":{"rendered":"

\u7d44\u7e54\u86cb\u767d\u53bb\u4e59\u91af\u9176\u6291\u5236\u5291\u53ca\u85e5\u7269\u7be9\u9078\u5206\u6790\u7814\u7a76<\/span><\/p>\n

\u7d44\u7e54\u86cb\u767d\u53bb\u4e59\u91af\u9176\uff0c\u662f\u4fc3\u4f7f\u7d44\u7e54\u86cb\u767dHistone\u53bb\u4e59\u91af\u5316\u7684\u4e00\u7a2e\u9175\u7d20\u3002\u7576\u7d44\u86cb\u767d\u53bb\u4e59\u91af\u9176<\/em>\u4e0d\u6b63\u5e38\u8868\u73fe\u6d3b\u6027\uff0c\u9020\u6210\u5176\u4f5c\u7528\u7684\u86cb\u767d\u8cea\u4e59\u91af\u5316\u7a0b\u5ea6\u5931\u53bb\u5e73\u8861\uff0c\u6703\u5c0e\u81f4\u8a31\u591a\u75be\u75c5\u5982\u764c\u75c7\u3001\u795e\u7d93\u9000\u5316\u6027\u75be\u75c5\u7b49\u3002\u76ee\u524d\uff0c\u7d44\u86cb\u767d<\/em>\u53bb\u4e59\u91af\u5316\u9176\u7684\u6291\u5236\u5291<\/em>\u5df2\u505a\u70ba\u6297\u816b\u7624\u7d30\u80de\u589e\u751f\u3001\u4fc3\u6210\u7d30\u80de\u51cb\u4ea1\u3001\u4fc3\u4f7f\u816b\u7624\u7d30\u80de\u5206\u5316\u3001\u4e26\u6291\u5236\u8840\u7ba1\u65b0\u751f\u4f5c\u7528\u3002\u56e0\u6b64HDACs \u70ba\u5177\u6709\u6f5b\u529b\u7684\u764c\u75c7\u6cbb\u7642\u6a19\u9776\u3002Biovision\u63d0\u4f9b\u4e00\u7cfb\u5217\u7d44\u7e54\u86cb\u767d\u53bb\u4e59\u91af\u9176\u6d3b\u6027\u5206\u6790(HDAC Activity assay)\u3001\u7d44\u7e54\u86cb\u767d\u53bb\u4e59\u91af\u9176\u6291\u5236\u5291\u5feb\u7be9\u5957\u7d44 (HDAC inhibitor screening assay kit)\u3001\u53ca\u7d44\u7e54\u86cb\u767d\u53bb\u4e59\u91af\u9176\u6291\u5236\u5291 (histone deacetylase inhibitors, HDACIs )\u7b49\u7cfb\u5217\u5546\u54c1\u3002<\/p>\n

HDAC6<\/strong>\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\uff08\u87a2\u5149\u6e2c\u5b9a\uff09 <\/strong>| <\/strong>\u8ca8\u865f<\/strong>K466-100<\/a><\/span><\/p>\n

HDAC6 Activity Assay Kit (Fluorometric)<\/em><\/strong><\/p>\n

\u7522\u54c1<\/strong>\u63cf\u8ff0\uff1a<\/strong>\u7d44\u86cb\u767d\u812b\u4e59\u91af\u91766\uff08HDAC6\uff0cEC 3.5.1.98\uff09\u662f\u812b\u4e59\u91af\u5316\u7d44\u86cb\u767d\u548c\u975e\u7d44\u86cb\u767d\u5e95\u7269\u7684\u03b5-N-\u4e59\u91af\u8cf4\u6c28\u9178\u7684IIb\u985eHDAC\u9176\u3002\u5b83\u662fHDAC\u7cfb\u5217\u7684\u7368\u7279\u6210\u54e1\uff0c\u56e0\u70ba\u5b83\u5305\u542b\u5169\u500b\u812b\u4e59\u91af\u9176\u7d50\u69cb\u57df\uff0c\u9019\u4e9b\u57df\u88ab\u63d0\u8b70\u5f7c\u6b64\u7368\u7acb\u904b\u4f5c\u3002 HDAC6\u53ef\u4ee5\u5728\u7d30\u80de\u6838\u548c\u7d30\u80de\u8cea\u4e4b\u9593\u7a7f\u68ad\uff0c\u901a\u904e\u8abf\u7bc0\u975e\u7d44\u86cb\u767d\u5e95\u7269\u7684\u4e59\u91af\u5316\u72c0\u614b\u4f86\u8868\u660e\u6f5b\u5728\u7684\u8d85\u6838\u529f\u80fd\u3002 HDAC6\u9084\u901a\u904e\u8abf\u7bc0\u71b1\u4f11\u514b\u86cb\u767d90\uff08Hsp90\uff09\u548c\u61c9\u6fc0\u9846\u7c92\u5f71\u97ff\u8f49\u9304\u548c\u7ffb\u8b6f\u3002\u5347\u9ad8\u7684HDAC6\u6d3b\u6027\u8207\u7d30\u80de\u904b\u52d5\u6027\u76f8\u95dc\uff0c\u4e26\u589e\u52a0\u03b1-\u5fae\u7ba1\u86cb\u767d\u812b\u4e59\u91af\u5316\uff0c\u5f9e\u800c\u5f71\u97ff\u764c\u7d30\u80de\u8f49\u79fb\u3002\u6b64\u5916\uff0cHDAC6\u57fa\u56e0\u7684\u7a81\u8b8a\u8207\u963f\u723e\u8328\u6d77\u9ed8\u6c0f\u75c5\u6709\u95dc\u3002 HDAC6\u9078\u64c7\u6027\u6291\u5236\u5291\u88ab\u8a8d\u70ba\u662f\u81ea\u8eab\u514d\u75ab\uff0c\u816b\u7624\u548c\u708e\u6027\u75be\u75c5\u7684\u6709\u5e0c\u671b\u7684\u9776\u6a19\u3002 BioVision\u7684HDAC6\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\u5c07HDAC6\u7684\u812b\u4e59\u91af\u9176\u6d3b\u6027\u7528\u65bc\u5408\u6210\u7684\u4e59\u91af\u5316\u80bd\u5e95\u7269\uff0c\u5c0e\u81f4AFC\u87a2\u5149\u5718\u7684\u91cb\u653e\uff0c\u5176\u53ef\u4ee5\u4f7f\u7528\u5e38\u898f\u7684\u9176\u6a19\u5100\u4f86\u5bb9\u6613\u5730\u5b9a\u91cf\u3002\u9019\u7a2e\u7c21\u55ae\u548c\u9ad8\u901a\u91cf\u7684\u9069\u61c9\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\u53ef\u7528\u65bc\u6aa2\u6e2c\u8907\u96dc\u751f\u7269\u6a23\u54c1\u4e2d\u7684HDAC6\u6d3b\u6027\uff08\u4f4e\u81f33U\uff09\u3002<\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK466-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Kit Summary<\/strong><\/td>\n\u2022 Detection method – Fluorescence (Ex\/Em 380\/490 nm)
\n\u2022 Species reactivity – Eukaryotes
\n\u2022 Applications – Measurement of HDAC6 activity in various biological samples.<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nFluorescence (Ex\/Em 380\/490 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nEukaryotes<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n\u2022 Rapid, high-throughput screening of drugs and novel ligands.
\n\u2022 Development of structure-activity relationship (SAR) models to predict HDAC6 interaction liability of novel compounds.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple, highly sensitive, high-throughput compatible
\n\u2022 Rapid screening of HDAC6 inhibitors
\n\u2022 Kit includes Tubacin, an HDAC6 inhibitor, and a stable, recombinant human HDAC6<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 HDAC6 Assay Buffer
\n\u2022 HDAC6 Lysis Buffer
\n\u2022 Human HDAC6 Positive Control
\n\u2022 HDAC6 Substrate
\n\u2022 Developer
\n\u2022 HDAC6 Inhibitor (Tubacin, 1 mM)
\n\u2022 AFC Standard (1 mM)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u76f8\u95dc\u8a66\u5291\"<\/a><\/strong>\u00a0HDAC\u6d3b\u6027(\u5448\u8272) \u5206\u6790\u5957\u7d44<\/a>\u00a0|\u00a0HDAC\u00a0\u6d3b\u6027(\u87a2\u5149)\u5206\u6790<\/a>\u00a0|\u00a0\uff08HDAC\uff09\u85e5\u7269\u7be9\u9078\u5206\u6790\u00a0|<\/a>\u00a0\u00a0HDAC3\u514d\u75ab\u6c89\u6fb1\uff08IP\uff09\u548c\u6d3b\u6027\u6e2c\u5b9a<\/a>\u00a0|\u00a0HDAC8\u6d3b\u6027\u6e2c\u5b9a<\/a>\u00a0|\u00a0HDAC6 (\u87a2\u5149)\u6d3b\u6027\u5206\u6790\u5957\u7d44<\/a>\u00a0|\u00a0HDAC1\u514d\u75ab\u6c89\u6fb1\uff08IP\uff09\u548c\u6d3b\u6027\u6e2c\u5b9a<\/a>\u00a0|\u00a0HDAC2\u514d\u75ab\u6c89\u6fb1\uff08IP\uff09\u548c\u6d3b\u6027\u6e2c\u5b9a<\/a>\u00a0|\u00a0HDAC6\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\uff08\u87a2\u5149\u6e2c\u5b9a\uff09<\/a><\/span><\/strong><\/p>\n

 <\/p>\n

 <\/p>\n

\u00a0<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

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