{"id":14904,"date":"2017-10-22T13:07:48","date_gmt":"2017-10-22T05:07:48","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=14904"},"modified":"2022-07-21T10:14:30","modified_gmt":"2022-07-21T02:14:30","slug":"hdac8%e7%b5%84%e8%9b%8b%e7%99%bd%e8%84%ab%e4%b9%99%e9%86%af%e9%85%b68%e6%b4%bb%e6%80%a7%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92-hdac8-activity-fluorometric-assay-kit%e8%b2%a8%e8%99%9fk348-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=14904","title":{"rendered":"HDAC8\u7d44\u86cb\u767d\u812b\u4e59\u91af\u91768\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2 | HDAC8 Activity Fluorometric Assay Kit\u8ca8\u865fK348-100"},"content":{"rendered":"

\u7d44\u7e54\u86cb\u767d\u53bb\u4e59\u91af\u9176\u6291\u5236\u5291\u53ca\u85e5\u7269\u7be9\u9078\u5206\u6790\u7814\u7a76<\/span><\/p>\n

\u7d44\u7e54\u86cb\u767d\u53bb\u4e59\u91af\u9176\uff0c\u662f\u4fc3\u4f7f\u7d44\u7e54\u86cb\u767dHistone\u53bb\u4e59\u91af\u5316\u7684\u4e00\u7a2e\u9175\u7d20\u3002\u7576\u7d44\u86cb\u767d\u53bb\u4e59\u91af\u9176<\/em>\u4e0d\u6b63\u5e38\u8868\u73fe\u6d3b\u6027\uff0c\u9020\u6210\u5176\u4f5c\u7528\u7684\u86cb\u767d\u8cea\u4e59\u91af\u5316\u7a0b\u5ea6\u5931\u53bb\u5e73\u8861\uff0c\u6703\u5c0e\u81f4\u8a31\u591a\u75be\u75c5\u5982\u764c\u75c7\u3001\u795e\u7d93\u9000\u5316\u6027\u75be\u75c5\u7b49\u3002\u76ee\u524d\uff0c\u7d44\u86cb\u767d<\/em>\u53bb\u4e59\u91af\u5316\u9176\u7684\u6291\u5236\u5291<\/em>\u5df2\u505a\u70ba\u6297\u816b\u7624\u7d30\u80de\u589e\u751f\u3001\u4fc3\u6210\u7d30\u80de\u51cb\u4ea1\u3001\u4fc3\u4f7f\u816b\u7624\u7d30\u80de\u5206\u5316\u3001\u4e26\u6291\u5236\u8840\u7ba1\u65b0\u751f\u4f5c\u7528\u3002\u56e0\u6b64HDACs \u70ba\u5177\u6709\u6f5b\u529b\u7684\u764c\u75c7\u6cbb\u7642\u6a19\u9776\u3002Biovision\u63d0\u4f9b\u4e00\u7cfb\u5217\u7d44\u7e54\u86cb\u767d\u53bb\u4e59\u91af\u9176\u6d3b\u6027\u5206\u6790(HDAC Activity assay)\u3001\u7d44\u7e54\u86cb\u767d\u53bb\u4e59\u91af\u9176\u6291\u5236\u5291\u5feb\u7be9\u5957\u7d44 (HDAC inhibitor screening assay kit)\u3001\u53ca\u7d44\u7e54\u86cb\u767d\u53bb\u4e59\u91af\u9176\u6291\u5236\u5291 (histone deacetylase inhibitors, HDACIs )\u7b49\u7cfb\u5217\u5546\u54c1\u3002<\/p>\n

HDAC8<\/strong>\u7d44\u86cb\u767d\u812b\u4e59\u91af\u91768\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2 <\/strong>\u00a0| <\/strong>\u8ca8\u865f<\/strong>K348-100<\/a><\/span><\/p>\n

HDAC8 Activity Fluorometric Assay Kit<\/em><\/strong><\/p>\n

\u7522\u54c1<\/strong>\u63cf\u8ff0\uff1a<\/strong>\u7d44\u86cb\u767d\u812b\u4e59\u91af\u9176\uff08HDACs\uff09\u4ee3\u8868\u4e86\u88ab\u8a8d\u70ba\u662f\u6838\u5c0f\u9ad4\u7d44\u86cb\u767d\u4e59\u91af\u5316\u7684\u95dc\u9375\u8abf\u7bc0\u5291\u7684\u5927\u91cf\u9176\uff0c\u9019\u662f\u63a7\u5236\u771f\u6838\u57fa\u56e0\u8f49\u9304\u7684\u4e3b\u8981\u4e8b\u4ef6\uff0c\u4e26\u88ab\u5206\u70ba\u4e09\u7d44\u3002 HDACs\u662f\u54fa\u4e73\u52d5\u7269\u767c\u80b2\u4e2d\u5177\u6709\u95dc\u9375\u4f5c\u7528\u7684\u8f49\u9304\u963b\u904f\u7269\u3002 \u4ed6\u5011\u88ab\u8a8d\u70ba\u53c3\u8207\u91cd\u8981\u7684\u751f\u7269\u6d3b\u6027\uff0c\u5982\u7d30\u80de\u5206\u5316\uff0c\u589e\u6b96\uff0c\u7d30\u80de\u51cb\u4ea1\u548c\u8870\u8001\u3002 \u5728BioVision\u7684HDAC-8\u6d3b\u6027\u8a66\u5291\u76d2\u4e2d\uff0cHDAC-8\u5c07\u5e95\u7269\uff08RHK\uff08Ac\uff09-K\uff08Ac\uff09-AFC\uff09\u812b\u4e59\u91af\u5316\uff0c\u7136\u5f8c\u9032\u884c\u9176\u88c2\u89e3\u91cb\u653e\u6dec\u6ec5\u7684\u87a2\u5149\u57fa\u5718\uff08AFC\uff09\uff0c\u5176\u53ef\u4ee5\u5728Em \/ Ex = 380 \/ 500nm\u3002 \u8a72\u8a66\u5291\u76d2\u63d0\u4f9b\u5feb\u901f\uff0c\u7c21\u55ae\uff0c\u9748\u654f\u548c\u53ef\u9760\u7684\u6e2c\u8a66\uff0c\u4e5f\u9069\u7528\u65bc\u7d14\u5316\uff0c\u514d\u75ab\u6c89\u6fb1\u548c\u91cd\u7d44\u6216\u8f49\u57fa\u56e0HDAC-8\u6a23\u54c1\u4e2dHDAC-8\u6d3b\u6027\u7684\u9ad8\u901a\u91cf\u6e2c\u91cf\u3002<\/p>\n

\"\"\"\"<\/p>\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK348-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nFluorescence (Ex\/Em = 380\/500 nm)<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nSuitable for high throughput measurement of HDAC8 activity in purified, immunoprecipitated and recombinant or genetically modified HDAC-8 samples.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure; takes ~ 60 min
\n\u2022 Fast and convenient
\n\u2022 Kit contains the necessary reagents for accurate measurement of HDAC-8 activity<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 HDAC-8 Assay Buffer
\n\u2022 HDAC-8 Substrate
\n\u2022 HDAC-8 Positive Control
\n\u2022 AFC Standard (1 mM)
\n\u2022 Developer
\n\u2022 Trichostatin A (10 \u03bcM)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n


\n\"\u76f8\u95dc\u8a66\u5291\"<\/a><\/strong>\u00a0HDAC\u6d3b\u6027(\u5448\u8272) \u5206\u6790\u5957\u7d44<\/a>\u00a0|\u00a0HDAC\u00a0\u6d3b\u6027(\u87a2\u5149)\u5206\u6790<\/a>\u00a0|\u00a0\uff08HDAC\uff09\u85e5\u7269\u7be9\u9078\u5206\u6790\u00a0|<\/a>\u00a0\u00a0HDAC3\u514d\u75ab\u6c89\u6fb1\uff08IP\uff09\u548c\u6d3b\u6027\u6e2c\u5b9a<\/a>\u00a0|\u00a0HDAC8\u6d3b\u6027\u6e2c\u5b9a<\/a>\u00a0|\u00a0HDAC6 (\u87a2\u5149)\u6d3b\u6027\u5206\u6790\u5957\u7d44<\/a>\u00a0|\u00a0HDAC1\u514d\u75ab\u6c89\u6fb1\uff08IP\uff09\u548c\u6d3b\u6027\u6e2c\u5b9a<\/a>\u00a0|\u00a0HDAC2\u514d\u75ab\u6c89\u6fb1\uff08IP\uff09\u548c\u6d3b\u6027\u6e2c\u5b9a<\/a>\u00a0|\u00a0HDAC6\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\uff08\u87a2\u5149\u6e2c\u5b9a\uff09<\/a><\/span><\/strong><\/p>\n

 <\/p>\n

\u00a0<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u7d44\u7e54\u86cb\u767d\u53bb\u4e59\u91af\u9176\u6291\u5236\u5291\u53ca\u85e5\u7269\u7be9\u9078\u5206\u6790\u7814\u7a76 \u7d44\u7e54\u86cb\u767d\u53bb\u4e59\u91af\u9176\uff0c\u662f\u4fc3\u4f7f\u7d44\u7e54\u86cb\u767dHistone\u53bb\u4e59\u91af\u5316\u7684\u4e00\u7a2e\u9175\u7d20\u3002\u7576 […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[457],"tags":[3079],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14904"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=14904"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14904\/revisions"}],"predecessor-version":[{"id":14922,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14904\/revisions\/14922"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=14904"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=14904"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=14904"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}