{"id":14901,"date":"2017-10-22T13:01:34","date_gmt":"2017-10-22T05:01:34","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=14901"},"modified":"2022-07-21T10:14:48","modified_gmt":"2022-07-21T02:14:48","slug":"hdac3%e5%85%8d%e7%96%ab%e6%b2%89%e6%be%b1%ef%bc%88ip%ef%bc%89%e5%92%8c%e6%b4%bb%e6%80%a7%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9f2042-250","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=14901","title":{"rendered":"HDAC3\u514d\u75ab\u6c89\u6fb1\uff08IP\uff09\u548c\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2 | \u8ca8\u865f2042-250"},"content":{"rendered":"

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\u7d44\u7e54\u86cb\u767d\u53bb\u4e59\u91af\u9176\uff0c\u662f\u4fc3\u4f7f\u7d44\u7e54\u86cb\u767dHistone\u53bb\u4e59\u91af\u5316\u7684\u4e00\u7a2e\u9175\u7d20\u3002\u7576\u7d44\u86cb\u767d\u53bb\u4e59\u91af\u9176<\/em>\u4e0d\u6b63\u5e38\u8868\u73fe\u6d3b\u6027\uff0c\u9020\u6210\u5176\u4f5c\u7528\u7684\u86cb\u767d\u8cea\u4e59\u91af\u5316\u7a0b\u5ea6\u5931\u53bb\u5e73\u8861\uff0c\u6703\u5c0e\u81f4\u8a31\u591a\u75be\u75c5\u5982\u764c\u75c7\u3001\u795e\u7d93\u9000\u5316\u6027\u75be\u75c5\u7b49\u3002\u76ee\u524d\uff0c\u7d44\u86cb\u767d<\/em>\u53bb\u4e59\u91af\u5316\u9176\u7684\u6291\u5236\u5291<\/em>\u5df2\u505a\u70ba\u6297\u816b\u7624\u7d30\u80de\u589e\u751f\u3001\u4fc3\u6210\u7d30\u80de\u51cb\u4ea1\u3001\u4fc3\u4f7f\u816b\u7624\u7d30\u80de\u5206\u5316\u3001\u4e26\u6291\u5236\u8840\u7ba1\u65b0\u751f\u4f5c\u7528\u3002\u56e0\u6b64HDACs \u70ba\u5177\u6709\u6f5b\u529b\u7684\u764c\u75c7\u6cbb\u7642\u6a19\u9776\u3002Biovision\u63d0\u4f9b\u4e00\u7cfb\u5217\u7d44\u7e54\u86cb\u767d\u53bb\u4e59\u91af\u9176\u6d3b\u6027\u5206\u6790(HDAC Activity assay)\u3001\u7d44\u7e54\u86cb\u767d\u53bb\u4e59\u91af\u9176\u6291\u5236\u5291\u5feb\u7be9\u5957\u7d44 (HDAC inhibitor screening assay kit)\u3001\u53ca\u7d44\u7e54\u86cb\u767d\u53bb\u4e59\u91af\u9176\u6291\u5236\u5291 (histone deacetylase inhibitors, HDACIs )\u7b49\u7cfb\u5217\u5546\u54c1\u3002<\/p>\n

HDAC3<\/strong>\u514d\u75ab\u6c89\u6fb1\uff08IP\uff09\u548c\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2 <\/strong>\u00a0| <\/strong>\u8ca8\u865f<\/strong>2042-250<\/a><\/span><\/p>\n

Only kit on the market for both IP and measuring activity of HDAC3<\/em><\/strong><\/p>\n

\u7522\u54c1<\/strong>\u63cf\u8ff0\uff1a<\/strong>\u7d44\u86cb\u767d\u812b\u4e59\u91af\u9176\uff08HDAC\uff09\u5728\u63a7\u5236\u7d30\u80de\u9031\u671f\u8abf\u7bc0\uff0c\u7d30\u80de\u5206\u5316\u548c\u7d44\u7e54\u767c\u80b2\u4e2d\u8d77\u8457\u4e2d\u5fc3\u4f5c\u7528\u3002 \u9019\u4e9b\u86cb\u767d\u8cea\u5728\u767c\u80b2\u548c\u751f\u7406\u5b78\u4e2d\u8d77\u95dc\u9375\u4f5c\u7528\uff0c\u7279\u5225\u662f\u5728\u5fc3\u81df\u548c\u795e\u7d93\u7cfb\u7d71\u4e2d\u3002 \u4ed6\u5011\u4e5f\u6df1\u5165\u7d30\u80de\u589e\u6b96\uff0c\u7d30\u80de\u9031\u671f\u548c\u7d30\u80de\u51cb\u4ea1\u3002 HDAC3\u4e3b\u8981\u5b9a\u4f4d\u5728\u7d30\u80de\u6838\u4e2d\uff0c\u4f46\u4e5f\u53ef\u4ee5\u5728\u7d30\u80de\u8cea\u548c\u8cea\u819c\u4e2d\u767c\u73fe\u3002 HDAC3 IP\u548c\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\u63d0\u4f9b\u57fa\u65bc\u6297\u9ad4\u7684\u65b9\u6cd5\uff0c\u5f9e\u7d30\u80de\u548c\u7d44\u7e54\u4e2d\u7279\u7570\u6027\u514d\u75ab\u6c89\u6fb1HDAC3\u8907\u5408\u7269\uff0c\u4e26\u4ee5\u87a2\u5149\u6e2c\u5b9a\u6cd5\u6e2c\u91cfHDAC3\u6d3b\u6027\u3002 HDA3\u4f7f\u7528HDAC3\u6297\u9ad4\u5f9e\u7d30\u80de\u6216\u6838\u63d0\u53d6\u7269\u4e2d\u514d\u75ab\u6c89\u6fb1\uff0c\u7136\u5f8c\u5728\u86cb\u767dA \/ G\u73e0\u4e0a\u6355\u7372\u8907\u5408\u7269\u3002 \u5c07\u514d\u75ab\u6c89\u6fb1\u7684\u8907\u5408\u7269\u8207HDAC\u5e95\u7269\u6eab\u80b2\u3002 \u53ea\u6709\u812b\u4e59\u91af\u5316\u5e95\u7269\u88ab\u986f\u5f71\u5291\u5207\u5272\u4ee5\u7522\u751f\u87a2\u5149\u5718\uff0c\u5176\u53ef\u4ee5\u4f7f\u7528\u87a2\u5149\u8b80\u677f\u5668\u5bb9\u6613\u5730\u5206\u6790\u3002<\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK344-25<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n25 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nFluorescence (Ex\/Em=368\/442 nm)<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nImmunoprecipitation of HDAC3 complex from cell & nuclear extract(s)
\nMeasurement of HDAC3 activity of immunoprecipitated complex\/purified enzyme
\nScreening for activators or inhibitors of HDAC3<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple, fast and convenient.
\n\u2022 Only kit on the market for both IP as well as measuring activity of HDAC3.<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 HDAC Assay Buffer
\n\u2022 Cell Lysis Buffer
\n\u2022 HDAC Substrate
\n\u2022 Developer
\n\u2022 AFC (7-amino-4-trifluoromethyl coumarin) Standard (1 mM)
\n\u2022 Rabbit HDAC2 Antibody (0.2 mg\/ml)
\n\u2022 Rabbit IgG (Control Antibody) (0.5 mg\/ml)
\n\u2022 Protein A\/G Sepharose Beads
\n\u2022 Positive Control (Jurkat Cell Lysate) (125 \u03bcg)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n


\n\"\u76f8\u95dc\u8a66\u5291\"<\/a><\/strong>\u00a0HDAC\u6d3b\u6027(\u5448\u8272) \u5206\u6790\u5957\u7d44<\/a>\u00a0|\u00a0HDAC\u00a0\u6d3b\u6027(\u87a2\u5149)\u5206\u6790<\/a>\u00a0|\u00a0\uff08HDAC\uff09\u85e5\u7269\u7be9\u9078\u5206\u6790\u00a0|<\/a>\u00a0\u00a0HDAC3\u514d\u75ab\u6c89\u6fb1\uff08IP\uff09\u548c\u6d3b\u6027\u6e2c\u5b9a<\/a>\u00a0|\u00a0HDAC8\u6d3b\u6027\u6e2c\u5b9a<\/a>\u00a0|\u00a0HDAC6 (\u87a2\u5149)\u6d3b\u6027\u5206\u6790\u5957\u7d44<\/a>\u00a0|\u00a0HDAC1\u514d\u75ab\u6c89\u6fb1\uff08IP\uff09\u548c\u6d3b\u6027\u6e2c\u5b9a<\/a>\u00a0|\u00a0HDAC2\u514d\u75ab\u6c89\u6fb1\uff08IP\uff09\u548c\u6d3b\u6027\u6e2c\u5b9a<\/a>\u00a0|\u00a0HDAC6\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\uff08\u87a2\u5149\u6e2c\u5b9a\uff09<\/a><\/span><\/strong><\/p>\n

 <\/p>\n

\u00a0<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

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