{"id":14895,"date":"2017-10-22T12:49:03","date_gmt":"2017-10-22T04:49:03","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=14895"},"modified":"2022-07-21T10:14:55","modified_gmt":"2022-07-21T02:14:55","slug":"hdac%e7%b5%84%e7%b9%94%e8%9b%8b%e7%99%bd%e5%8e%bb%e4%b9%99%e9%86%af%e9%85%b6%e6%b4%bb%e6%80%a7%e5%88%86%e6%9e%90%e5%a5%97%e7%b5%84-hdac-activity-colorimetric-assay-kit%e8%b2%a8%e8%99%9fk331-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=14895","title":{"rendered":"HDAC\u7d44\u7e54\u86cb\u767d\u53bb\u4e59\u91af\u9176\u6d3b\u6027\u5206\u6790\u5957\u7d44 | HDAC Activity Colorimetric Assay Kit\u8ca8\u865fK331-100"},"content":{"rendered":"

\u7d44\u7e54\u86cb\u767d\u53bb\u4e59\u91af\u9176\u6291\u5236\u5291\u53ca\u85e5\u7269\u7be9\u9078\u5206\u6790\u7814\u7a76<\/strong><\/span><\/p>\n

\u7d44\u7e54\u86cb\u767d\u53bb\u4e59\u91af\u9176\uff0c\u662f\u4fc3\u4f7f\u7d44\u7e54\u86cb\u767dHistone\u53bb\u4e59\u91af\u5316\u7684\u4e00\u7a2e\u9175\u7d20\u3002\u7576\u7d44\u86cb\u767d\u53bb\u4e59\u91af\u9176<\/em>\u4e0d\u6b63\u5e38\u8868\u73fe\u6d3b\u6027\uff0c\u9020\u6210\u5176\u4f5c\u7528\u7684\u86cb\u767d\u8cea\u4e59\u91af\u5316\u7a0b\u5ea6\u5931\u53bb\u5e73\u8861\uff0c\u6703\u5c0e\u81f4\u8a31\u591a\u75be\u75c5\u5982\u764c\u75c7\u3001\u795e\u7d93\u9000\u5316\u6027\u75be\u75c5\u7b49\u3002\u76ee\u524d\uff0c\u7d44\u86cb\u767d<\/em>\u53bb\u4e59\u91af\u5316\u9176\u7684\u6291\u5236\u5291<\/em>\u5df2\u505a\u70ba\u6297\u816b\u7624\u7d30\u80de\u589e\u751f\u3001\u4fc3\u6210\u7d30\u80de\u51cb\u4ea1\u3001\u4fc3\u4f7f\u816b\u7624\u7d30\u80de\u5206\u5316\u3001\u4e26\u6291\u5236\u8840\u7ba1\u65b0\u751f\u4f5c\u7528\u3002\u56e0\u6b64HDACs \u70ba\u5177\u6709\u6f5b\u529b\u7684\u764c\u75c7\u6cbb\u7642\u6a19\u9776\u3002Biovision\u63d0\u4f9b\u4e00\u7cfb\u5217\u7d44\u7e54\u86cb\u767d\u53bb\u4e59\u91af\u9176\u6d3b\u6027\u5206\u6790(HDAC Activity assay)\u3001\u7d44\u7e54\u86cb\u767d\u53bb\u4e59\u91af\u9176\u6291\u5236\u5291\u5feb\u7be9\u5957\u7d44 (HDAC inhibitor screening assay kit)\u3001\u53ca\u7d44\u7e54\u86cb\u767d\u53bb\u4e59\u91af\u9176\u6291\u5236\u5291 (histone deacetylase inhibitors, HDACIs )\u7b49\u7cfb\u5217\u5546\u54c1\u3002<\/p>\n

HDAC<\/strong>\u7d44\u7e54\u86cb\u767d\u53bb\u4e59\u91af\u9176\u6d3b\u6027\u5206\u6790\u5957\u7d44 \u00a0| <\/strong>\u8ca8\u865fK331-100<\/strong><\/a><\/span><\/p>\n

HDAC Activity Colorimetric Assay Kit, Two step<\/em><\/strong><\/p>\n

\u7522\u54c1<\/strong>\u63cf\u8ff0\uff1a<\/strong>\u7d44\u86cb\u767d\u812b\u4e59\u9170\u9176\uff08HDAC\uff09\u7684\u6291\u5236\u6d89\u53ca\u8abf\u7bc0\u8f49\u9304\u548c\u8a98\u5c0e\u764c\u7d30\u80de\u51cb\u4ea1\u6216\u5206\u5316\u3002\u7136\u800c\uff0c\u7531\u65bc\u7f3a\u4e4f\u7528\u65bc\u5206\u6790HDAC\u6d3b\u6027\u7684\u65b9\u4fbf\u7684\u5de5\u5177\uff0c\u7be9\u9078HDAC\u6291\u5236\u5316\u5408\u7269\u5728\u904e\u53bb\u88ab\u8b49\u660e\u662f\u56f0\u96e3\u7684\u3002\u65b0\u7684\u6bd4\u8272HDAC\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u5feb\u901f\u4fbf\u6377\u7684\u5448\u8272\u6cd5\uff0c\u53ef\u4ee5\u6d88\u9664\u50b3\u7d71\u6e2c\u5b9a\u4e2d\u4f7f\u7528\u7684\u653e\u5c04\u6027\uff0c\u8403\u53d6\u6216\u8272\u8b5c\u7e41\u7463\u4e4b\u6b65\u9a5f\u3002\u65b0\u65b9\u6cd5\u53ea\u9700\u8981\u5169\u500b\u7c21\u55ae\u7684\u6b65\u9a5f\uff0c\u5169\u500b\u90fd\u5728\u76f8\u540c\u7684\u5fae\u91cf\u6ef4\u5b9a\u677f\u4e0a\u9032\u884c\u3002\u9996\u5148\uff0c\u5c07\u5305\u542b\u4e59\u91af\u5316\u8cf4\u6c28\u9178\u5074\u93c8\u7684HDAC\u6bd4\u8272\u5e95\u7269\u8207\u542b\u6709HDAC\u6d3b\u6027\u7684\u6a23\u54c1\uff08\u4f8b\u5982HeLa\u6838\u63d0\u53d6\u7269\u6216\u60a8\u81ea\u5df1\u7684\u6a23\u54c1\uff09\u4e00\u8d77\u5b75\u80b2\u3002\u5e95\u7269\u7684\u812b\u4e59\u91af\u5316\u5c0d\u5e95\u7269\u654f\u611f\uff0c\u56e0\u6b64\u5728\u7b2c\u4e8c\u6b65\u4e2d\uff0c\u8207\u8cf4\u6c28\u9178\u986f\u5f71\u5291\u76f8\u7d50\u5408\u7522\u751f\u9aee\u8272\u5718\u3002\u53ef\u4ee5\u4f7f\u7528ELISA\u677f\u8b80\u6578\u5668\u6216\u5206\u5149\u5149\u5ea6\u8a08\u8f15\u9b06\u5206\u6790\u9aee\u8272\u5718\u3002\u8a72\u6e2c\u5b9a\u975e\u5e38\u9069\u5408\u65bc\u9ad8\u901a\u91cf\u7be9\u9078\u61c9\u7528\u3002 HDAC\u6291\u5236\u5291\u548c\u6297\u9ad4\u4e5f\u53ef\u55ae\u7368\u4f7f\u7528\u3002<\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK331-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (400 or 405 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nWell suited for high throughput applications. HDAC inhibitors and substrates and related antibodies are also available separately.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple two-step procedure; takes around than 1 hour
\n\u2022 Fast and convenient
\n\u2022 The assay method eliminates radioactivity\/extractions\/ and\/or chromatography as used in the traditional assays.<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 HDAC Substrate [Ac-Lys(Ac)-pNA, 10 mM]
\n\u2022 10X HDAC Assay Buffer
\n\u2022 Lysine Developer
\n\u2022 HDAC Inhibitor (Trichostatin A, 1 mM)
\n\u2022 HeLa Nuclear Extract (5 mg\/ml)
\n\u2022 Deacetylated Standard (Ac-Lys-pNA, 10 mM)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u76f8\u95dc\u8a66\u5291\"<\/a><\/strong>\u00a0HDAC\u6d3b\u6027(\u5448\u8272) \u5206\u6790\u5957\u7d44<\/a>\u00a0|\u00a0HDAC\u00a0\u6d3b\u6027(\u87a2\u5149)\u5206\u6790<\/a>\u00a0|\u00a0\uff08HDAC\uff09\u85e5\u7269\u7be9\u9078\u5206\u6790\u00a0|<\/a>\u00a0\u00a0HDAC3\u514d\u75ab\u6c89\u6fb1\uff08IP\uff09\u548c\u6d3b\u6027\u6e2c\u5b9a<\/a>\u00a0|\u00a0HDAC8\u6d3b\u6027\u6e2c\u5b9a<\/a>\u00a0|\u00a0HDAC6 (\u87a2\u5149)\u6d3b\u6027\u5206\u6790\u5957\u7d44<\/a>\u00a0|\u00a0HDAC1\u514d\u75ab\u6c89\u6fb1\uff08IP\uff09\u548c\u6d3b\u6027\u6e2c\u5b9a<\/a>\u00a0|\u00a0HDAC2\u514d\u75ab\u6c89\u6fb1\uff08IP\uff09\u548c\u6d3b\u6027\u6e2c\u5b9a<\/a>\u00a0|\u00a0HDAC6\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\uff08\u87a2\u5149\u6e2c\u5b9a\uff09<\/a><\/span><\/strong><\/p>\n

\u00a0\u5f15\u7528\u6587\u737b<\/strong><\/p>\n

\n
Tzeng, Haiyue et al. (2016) Antioxidant-Rich Extract from Plantaginis Semen Ameliorates Diabetic Retinal Injury in a Streptozotocin-Induced Diabetic Rat Model,\u00a0Nutrients. 2016 Sep 18;8(9).<\/a><\/div>\n<\/div>\n
\n
Gurav et al., Slc5a8, a Na+-coupled high-affinity transporter for short-chain fatty acids, is a conditional tumour suppressor in colon that protects against colitis and colon cancer under low-fibre dietary conditions.\u00a0Biochem. J.,\u00a0Jul 2015; 469: 267 – 278.<\/a><\/div>\n<\/div>\n
\n
Yang et al., PARP-1 Mediates LPS-Induced HMGB1 Release by Macrophages through Regulation of HMGB1 Acetylation.J. Immunol.,\u00a0Dec 2014; 193: 6114 – 6123.<\/a><\/div>\n<\/div>\n
\n
Zhang et al., Specific inhibition of HDAC4 in cardiac progenitor cells enhances myocardial repairs.\u00a0Am J Physiol Cell Physiol,\u00a0Aug 2014; 307: C358 – C372.<\/a><\/div>\n<\/div>\n
\n
Aslan et al., Histone deacetylase 6-mediated deacetylation of \u03b1-tubulin coordinates cytoskeletal and signaling events during platelet activation.\u00a0Am J Physiol Cell Physiol, Dec 2013; 305: C1230 – C1239.<\/a><\/div>\n<\/div>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

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