{"id":14819,"date":"2017-10-19T13:35:49","date_gmt":"2017-10-19T05:35:49","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=14819"},"modified":"2022-07-21T10:15:52","modified_gmt":"2022-07-21T02:15:52","slug":"total-pde%e7%b8%bd%e7%a3%b7%e9%85%b8%e4%ba%8c%e9%85%af%e9%85%b6%e6%b4%bb%e6%80%a7%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fa1274","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=14819","title":{"rendered":"PDE\u7e3d\u78f7\u9178\u4e8c\u916f\u9176\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2 | \u8ca8\u865fA1274"},"content":{"rendered":"

Total PDE<\/strong>\u7e3d\u78f7\u9178\u4e8c\u916f\u9176\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\uff08<\/strong>\u87a2<\/strong>\u5149\uff09<\/strong> | <\/strong>\u8ca8\u865f<\/strong>A1274<\/a><\/span><\/p>\n

Highly Sensitive, minimal sample preparation required<\/em><\/strong><\/p>\n

\u7522\u54c1<\/strong>\u63cf\u8ff0\uff1a<\/strong>\u78f7\u9178\u4e8c\u916f\u9176\uff08Phosphodiesterase , PDE\uff09\u662f\u4e00\u7a2e\u5207\u5272\u78f7\u9178\u4e8c\u916f\u9375\u7684\u9176\u3002\u9019\u4e9b\u5305\u62ec\u74b0\u6838\u82f7\u9178\u78f7\u9178\u4e8c\u916f\u9176\uff0c\u78f7\u8102\u9176C\u548cD\uff0c\u6838\u7cd6\u6838\u9178\u9176\uff0cDNA\u9176\u548c\u4e00\u4e9b\u9650\u5236\u6027\u5167\u5207\u6838\u9178\u9176\u3002\u78f7\u9178\u4e8c\u916f\u9176\u662f\u7528\u65bc\u5404\u7a2e\u751f\u7269\u529f\u80fd\u7684\u91cd\u8981\u9176\uff0c\u5305\u62ec\u7b2c\u4e8c\u4fe1\u4f7f\u5206\u5b50\u5982cAMP \/ cGMP\u7684\u4ee3\u8b1d\uff08\u56e0\u6b64\u8abf\u7bc0\u5176\u7d30\u80de\u5167\u6c34\u5e73\uff09\u3002\u5728\u7d30\u80de\u51cb\u4ea1\u904e\u7a0b\u4e2d\uff0cPDE\u6d3b\u6027\u88ab\u7ffb\u8b6f\u6210\u86cb\u767d\u8cea\u548cDNA\u5f8c\uff0c\u5c0e\u81f4mRNA\u7684\u964d\u89e3\u3002\u4e00\u4e9bPDE\u7570\u69cb\u9ad4\u5982PDE 5\u548cPDE 11\u662f\u6cbb\u7642\u9776\u9ede\uff0c\u56e0\u70ba\u5b83\u5011\u5206\u5225\u5728\u5fc3\u8840\u7ba1\u7cfb\u7d71\u548c\u816b\u7624\u5f62\u6210\u4e2d\u8d77\u4f5c\u7528\u3002 BioVision\u7684PDE\u6e2c\u5b9a\u8a66\u5291\u76d2\u662f\u7528\u65bc\u52d5\u614b\u6e2c\u91cf\u7e3d\u78f7\u9178\u4e8c\u916f\u9176\u6d3b\u6027\u7684\u57fa\u65bc\u87a2\u5149\u8a66\u7247\u7684\u6e2c\u5b9a\u6cd5\u3002\u5b83\u57fa\u65bc\u901a\u904e\u78f7\u9178\u4e8c\u916f\u9176\u88c2\u89e3\u57fa\u65bc\u9999\u8c46\u7d20\u7684\u5408\u6210\u5e95\u7269\u4ee5\u7522\u751f\u5728Ex \/ Em = 370 \/ 450nm\u8655\u767c\u87a2\u5149\u7684\u7522\u7269\u3002\u5b83\u4e0d\u4f9d\u8cf4\u65bc\u78f7\u9178\u4e8c\u916f\u9176\u4f5c\u7528\u91cb\u653e\u7684\u78f7\u9178\u9e7d\u7684\u5b9a\u91cf\uff0c\u5c31\u50cf\u76ee\u524d\u4f7f\u7528\u7684\u5176\u4ed6\u50b3\u7d71\u65b9\u6cd5\u4e00\u6a23\u3002\u56e0\u6b64\uff0c\u6a23\u54c1\u80cc\u666f\u53ef\u4ee5\u5ffd\u7565\u4e0d\u8a08\uff0c\u4e26\u4e14\u4e0d\u6703\u5f71\u97ff\u6e2c\u5b9a\u3002\u5e95\u7269\u5c0d\u78f7\u9178\u4e8c\u916f\u9176\u662f\u7279\u7570\u6027\u7684\uff0c\u4e26\u4e14\u4e0d\u88ab\u4f5c\u7528\u65bc\u7fa7\u57fa\u916f\u6216\u78f7\u9178\u9176\u7684\u9176\u88c2\u89e3\u3002\u8a72\u6e2c\u5b9a\u6cd5\u53ef\u4ee5\u6aa2\u6e2c\u4f4e\u81f30.25\u03bcU\u7684\u7e3dPDE\u3002<\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK927-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Kit Summary<\/strong><\/td>\n\u2022 Detection method – Fluorescence (Ex\/Em 370\/450 nm)
\n\u2022 Species reactivity – Mammalian
\n\u2022 Applications – This assay has a detection sensitivity as low as 0.25 \u00b5U.<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nFluorescence (Ex\/Em 370\/450 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nDetection and quantificiation of total phosphodiesterase activity in biological samples<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple, highly sensitive, high-throughput compatible
\n\u2022 Detection and quantificiation of total phosphodiesterase activity in biological samples
\n\u2022 This assay has a detection sensitivity as low as 0.25 \u00b5U.<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 PDE Assay Buffer
\n\u2022 PDE Substrate
\n\u2022 Coumarin Standard (50 \u00b5M)
\n\u2022 PDE Positive Control<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u76f8\u95dc\u8a66\u5291\"\u00a0<\/span><\/strong><\/a>Apremilast<\/a>\u00a0 |\u00a0PDE\u7e3d\u78f7\u9178\u4e8c\u916f\u9176\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0Roflumilast<\/a>\u00a0|\u00a0Zaprinast<\/a>\u00a0|\u00a0IBMX<\/a>\u00a0|\u00a0Cilostazol\u00a0<\/a>\u00a0|\u00a0Milrinone<\/a>\u00a0|\u00a0<\/strong><\/strong><\/p>\n

\u00a0<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

Total PDE\u7e3d\u78f7\u9178\u4e8c\u916f\u9176\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\uff08\u87a2\u5149\uff09 | \u8ca8\u865fA1274 Highly Sensitive, […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[3089],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14819"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=14819"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14819\/revisions"}],"predecessor-version":[{"id":14871,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14819\/revisions\/14871"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=14819"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=14819"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=14819"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}