{"id":14695,"date":"2017-10-18T13:14:27","date_gmt":"2017-10-18T05:14:27","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=14695"},"modified":"2022-07-21T10:25:32","modified_gmt":"2022-07-21T02:25:32","slug":"tnf-a-growth-factors-and-cytokines-293%e7%b4%b0%e8%83%9e%e8%a1%a8%e9%81%94growth-factors%e8%b2%a8%e8%99%9fz103077","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=14695","title":{"rendered":"TNF-a \u91cd\u7d44\u86cb\u767d| 293\u7d30\u80de\u8868\u9054Growth Factors\u8ca8\u865fZ103077"},"content":{"rendered":"

\"ABM\u3010<\/span><\/strong>Growth factors&cytokines<\/strong>\u7d30\u80de\u751f\u9577\u56e0\u5b50<\/strong>\u3011 CHO cell\/<\/strong>HEK293<\/strong>\u00a0\/E.coli<\/strong>\u8868\u73fe\u7cfb\u7d71<\/strong><\/span><\/span><\/p>\n

Growth factors\u751f\u9577\u56e0\u5b50\u548ccytokines\u7d30\u80de\u56e0\u5b50\uff0c\u662f\u5929\u7136\u5b58\u5728\u7684signaling molecules\u4fe1\u865f\u5206\u5b50\uff0c\u5b83\u5011\u662f\u9ad8\u7b49\u751f\u7269\u7d30\u80de\u4e4b\u9593\u651c\u5e36\u8abf\u7bc0\u4fe1\u865f\u7684\u95dc\u9375\u56e0\u7d20\u3002\u5118\u7ba1\u7d30\u80de\u56e0\u5b50\u4e3b\u8981\u8207immune system housekeeping\u514d\u75ab\u7cfb\u7d71\u76f8\u95dc\uff0c\u751f\u9577\u56e0\u5b50\u5728\u751f\u7269\u9ad4\u7684\u767c\u80b2\u548c\u6210\u719f\u968e\u6bb5\u8abf\u7bc0\u5177\u5ee3\u6cdb\u7684\u904e\u7a0b\uff1b\u4e0d\u53d7\u63a7\u5236\u7684\u751f\u9577\u56e0\u5b50\u6fc0\u6d3b\u901a\u5e38\u8207oncogenesis\u816b\u7624\u767c\u751f\u76f8\u95dc\u3002\u751f\u9577\u56e0\u5b50\u548c\u7d30\u80de\u56e0\u5b50\u5c0d\u65bc\u7d30\u80de\u7684\u8a31\u591a\u529f\u80fd\u662f\u4e0d\u53ef\u7f3a\u5c11\u7684\u3002\u50b3\u7d71\u7684\u7d30\u80de\u751f\u7269\u5b78\u5be6\u9a57\u4f7f\u7528\u52d5\u7269\u8840\u6e05\u4f5c\u70ba\u6b63\u5e38\u7d30\u80de\u4ee3\u8b1d\u6240\u9700\u7684\u751f\u9577\u56e0\u5b50\u548c\u71df\u990a\u4f86\u6e90\u3002\u4f46\u662f\uff0c\u4f7f\u7528\u52d5\u7269\u8840\u6e05\uff0c\u5bb9\u6613\u589e\u52a0\u75c5\u6bd2\uff0c\u652f\u539f\u9ad4\u548c\u670a\u75c5\u6bd2\u6c61\u67d3\u7684\u98a8\u96aa\uff1b\u8207\u8840\u6e05\u6536\u96c6\u76f8\u95dc\u7684\u9ad8\u6210\u672c\u548c\u502b\u7406\u554f\u984c\uff1b\u5c0d\u53ef\u80fd\u9084\u542b\u6709\u751f\u9577\u6291\u5236\u5291\u3002\u9ad8\u54c1\u8ceaGrowth factors&cytokines\u7522\u54c1\u5177\u6709\u4ee5\u4e0b\u7279\u9ede\uff1a(A) \u56b4\u683c\u689d\u4ef6\u4e0b\u7684\u9ad8\u7d14\u5ea6\u751f\u7522\u78ba\u4fdd\u8cea\u91cf\u4fdd\u6301\u4e00\u81f4\uff0c\u6bcf\u6279\u6279\u6b21\u7d14\u5ea6\u226595\uff05\u901a\u904eSDS-PAGE. (B) \u5167\u6bd2\u7d20\u6700\u5c0f – Limulus Amebocyte Lysate (LAL) assay\u3002\u4f4e\u65bc1 EU\/ug recombinant protein\u3002(C) \u78ba\u4fdd\u6bcf\u4e00\u6279\u751f\u7269\u6d3b\u6027\u3002<\/p>\n

\"\"<\/p>\n

TNF-a Growth Factors and Cytokines\u00a0 <\/strong>| \u00a0293<\/strong>\u7d30\u80de\u8868\u9054Growth Factors<\/strong>\u8ca8\u865f<\/strong>Z103077<\/strong><\/a><\/span>
\nTNF, TNFA, Tumor necrosis factor, Cachectin, Tumor necrosis factor ligand superfamily member 2, TNF-a<\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> \u5de8\u566c\u7d30\u80de\uff0c\u55ae\u6838\u7d30\u80de\uff0c\u55dc\u4e2d\u6027\u7c92\u7d30\u80de\uff0cT\u7d30\u80de\uff0c\u7d30\u80de\u8102\u591a\u91a3\u523a\u6fc0\u5f8c\u7684\u81ea\u7136\u6bba\u50b7\u7d30\u80de\u5206\u6ccc\u816b\u7624\u58de\u6b7b\u56e0\u5b50\u3002 \u523a\u6fc0\u7684\u5916\u5468\u55dc\u4e2d\u6027\u7c92\u7d30\u80de\uff0c\u9084\u6709\u672a\u523a\u6fc0\u7684\u7d30\u80de\uff0c\u9084\u6709\u8a31\u591a\u8f49\u5316\u7684\u7d30\u80de\u7cfb\uff0c\u661f\u5f62\u81a0\u8cea\u7d30\u80de\uff0c\u5c0f\u81a0\u8cea\u7d30\u80de\uff0c\u5e73\u6ed1\u808c\u7d30\u80de\u548c\u6210\u7e96\u7dad\u7d30\u80de\u4e5f\u5206\u6cccTNF\u3002 \u4eba\u4e73\u4e5f\u542b\u6709\u9019\u500b\u56e0\u7d20\u3002 \u8868\u9054CD4\u5206\u6cccTNF-\u03b1\u7684\u7d30\u80de\uff0c\u800cCD8\uff08+\uff09\u7d30\u80de\u5206\u6ccc\u5f88\u5c11\u6216\u4e0d\u5206\u6cccTNF-\u03b1\u3002 TNF-\u03b1\u7684\u5408\u6210\u7531\u5305\u62ec\u5e72\u64fe\u7d20\uff0cIL-2\uff0cGM-CSF\uff0cSP\uff0c\u7de9\u6fc0\u80bd\uff0c\u514d\u75ab\u8907\u5408\u7269\uff0c\u74b0\u52a0\u6c27\u9176\u6291\u88fd\u5291\u548cPAF\uff08\u8840\u5c0f\u677f\u6d3b\u5316\u56e0\u5b50\uff09\u5728\u5167\u7684\u8a31\u591a\u4e0d\u540c\u523a\u6fc0\u7269\u8a98\u5c0e\u3002 \u5df2\u986f\u793a\u5728\u4eba293\u7d30\u80de\u4e2d\u8868\u9054\u7684\u91cd\u7d44\u4ebaTNF\u4e3b\u8981\u662f\u975e\u5171\u50f9\u9023\u63a5\u7684\u540c\u4e09\u805a\u7269\uff0c\u5206\u5b50\u91cf\u70ba51kDa\u3002\u00a0<\/strong><\/p>\n

\"\u76f8\u95dc\u8a66\u5291\"<\/strong>\u00a0aFGF\u91cd\u7d44\u86cb\u767d<\/a>\u00a0|\u00a0IL-2<\/strong>\u91cd\u7d44\u86cb\u767d<\/strong>\u00a0<\/a>\u00a0|\u00a0\u5e79\u7d30\u80de\u56e0\u5b50\uff08KITLG\uff09<\/a>\u00a0|\u00a0\u91cd\u7d44\u4eba\u767d\u8840\u75c5\u6291\u5236\u56e0\u5b50\uff08LIF\uff09<\/a>\u00a0|\u00a0\u91cd\u7d44\u4ebaHuman Noggin (NOG)\u00a0<\/a>\u00a0|\u00a0TNF-a<\/a>\u00a0 |\u00a0BMP2\u00a0<\/a><\/strong><\/span><\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Gene Symbol<\/strong><\/td>\nTNF<\/td>\n<\/tr>\n
Gene ID<\/strong><\/td>\n7124<\/td>\n<\/tr>\n
Accession No.<\/strong><\/td>\nP01375<\/td>\n<\/tr>\n
Source<\/strong><\/td>\nHEK293<\/td>\n<\/tr>\n
Appearance<\/strong><\/td>\nLyophilized Powder<\/td>\n<\/tr>\n
Molecular Weight<\/strong><\/td>\n17 kDa, non-disulfide bonded homotrimer, glycosylated<\/td>\n<\/tr>\n
Endotoxin Level<\/strong><\/td>\n<1 EU\/\u00b5g of recombinant protein as determined by the LAL method<\/td>\n<\/tr>\n
Purity<\/strong><\/td>\n>95% as determined by SDS-PAGE<\/td>\n<\/tr>\n
Bioactivity<\/strong><\/td>\nThe EC(50) as determined by the dose-dependent cytotoxity of the TNF alpha sensitive cell line L-929 in the presence of Actinomycin D was found to be \u2264 1 ng\/mL.<\/td>\n<\/tr>\n
Formulation<\/strong><\/td>\n1x PBS<\/td>\n<\/tr>\n
Reconstitution<\/strong><\/td>\nA quick spin of the vial followed by reconstitution in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Product<\/strong><\/td>\nSource<\/strong><\/td>\nSize<\/strong><\/td>\nCat. No.<\/strong><\/td>\n<\/tr>\n
Recombinant Human TNF<\/a><\/td>\nHEK293<\/td>\n10 \u00b5g<\/td>\nZ103075<\/td>\n<\/tr>\n
Recombinant Human TNF<\/a><\/td>\nHEK293<\/td>\n100 \u00b5g<\/td>\nZ103077<\/td>\n<\/tr>\n
Recombinant Mouse TNF<\/a><\/td>\nE. coli<\/em><\/td>\n50 \u00b5g<\/td>\nZ200215<\/td>\n<\/tr>\n
Recombinant Rat TNF<\/a><\/td>\nE. coli<\/em><\/td>\n20 \u00b5g<\/td>\nZ300065<\/td>\n<\/tr>\n
Recombinant Human TNF<\/a><\/td>\nCHO cells<\/td>\n50 \u00b5g<\/td>\nZ101385<\/td>\n<\/tr>\n
Recombinant Human TNFRSF1A<\/a><\/td>\nE. coli<\/em><\/td>\n20 \u00b5g<\/td>\nZ102235<\/td>\n<\/tr>\n
Recombinant Mouse TNF<\/a><\/td>\nE. coli<\/em><\/td>\n1.0 mg<\/td>\nZ200219<\/td>\n<\/tr>\n
Recombinant Rat TNF<\/a><\/td>\nE. coli<\/em><\/td>\n1.0 mg<\/td>\nZ300069<\/td>\n<\/tr>\n
Recombinant Human TNFRSF1A<\/a><\/td>\nE. coli<\/em><\/td>\n1.0 mg<\/td>\nZ102239<\/td>\n<\/tr>\n
Recombinant Rat TNF<\/a><\/td>\nE. coli<\/em><\/td>\n100 \u00b5g<\/td>\nZ300067<\/td>\n<\/tr>\n
Recombinant Mouse TNF<\/a><\/td>\nE. coli<\/em><\/td>\n100 \u00b5g<\/td>\nZ200217<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u3010Growth factors&cytokines\u7d30\u80de\u751f\u9577\u56e0\u5b50\u3011 CHO cell\/HEK293\u00a0\/E […]<\/p>\n","protected":false},"author":3,"featured_media":14679,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[139],"tags":[3106],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14695"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=14695"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14695\/revisions"}],"predecessor-version":[{"id":14714,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14695\/revisions\/14714"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/14679"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=14695"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=14695"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=14695"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}