{"id":14683,"date":"2017-10-18T12:00:23","date_gmt":"2017-10-18T04:00:23","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=14683"},"modified":"2022-07-21T10:26:01","modified_gmt":"2022-07-21T02:26:01","slug":"%e9%87%8d%e7%b5%84%e4%ba%ba%e7%99%bd%e8%a1%80%e7%97%85%e6%8a%91%e5%88%b6%e5%9b%a0%e5%ad%90%ef%bc%88lif%ef%bc%89-cho-cell%e8%a1%a8%e7%8f%be%e8%9b%8b%e7%99%bdabm%e8%b2%a8%e8%99%9fz101345","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=14683","title":{"rendered":"\u91cd\u7d44\u4eba\u767d\u8840\u75c5\u6291\u5236\u56e0\u5b50\uff08LIF\uff09 | Leukemia Inhibitory Factor ABM\u8ca8\u865fZ101345"},"content":{"rendered":"

\"ABM\u3010<\/span><\/strong>Growth factors&cytokines<\/strong>\u7d30\u80de\u751f\u9577\u56e0\u5b50<\/strong>\u3011 CHO cell\/<\/strong>HEK293<\/strong>\u00a0\/E.coli<\/strong>\u8868\u73fe\u7cfb\u7d71<\/strong><\/span><\/span><\/p>\n

Growth factors\u751f\u9577\u56e0\u5b50\u548ccytokines\u7d30\u80de\u56e0\u5b50\uff0c\u662f\u5929\u7136\u5b58\u5728\u7684signaling molecules\u4fe1\u865f\u5206\u5b50\uff0c\u5b83\u5011\u662f\u9ad8\u7b49\u751f\u7269\u7d30\u80de\u4e4b\u9593\u651c\u5e36\u8abf\u7bc0\u4fe1\u865f\u7684\u95dc\u9375\u56e0\u7d20\u3002\u5118\u7ba1\u7d30\u80de\u56e0\u5b50\u4e3b\u8981\u8207immune system housekeeping\u514d\u75ab\u7cfb\u7d71\u76f8\u95dc\uff0c\u751f\u9577\u56e0\u5b50\u5728\u751f\u7269\u9ad4\u7684\u767c\u80b2\u548c\u6210\u719f\u968e\u6bb5\u8abf\u7bc0\u5177\u5ee3\u6cdb\u7684\u904e\u7a0b\uff1b\u4e0d\u53d7\u63a7\u5236\u7684\u751f\u9577\u56e0\u5b50\u6fc0\u6d3b\u901a\u5e38\u8207oncogenesis\u816b\u7624\u767c\u751f\u76f8\u95dc\u3002\u751f\u9577\u56e0\u5b50\u548c\u7d30\u80de\u56e0\u5b50\u5c0d\u65bc\u7d30\u80de\u7684\u8a31\u591a\u529f\u80fd\u662f\u4e0d\u53ef\u7f3a\u5c11\u7684\u3002\u50b3\u7d71\u7684\u7d30\u80de\u751f\u7269\u5b78\u5be6\u9a57\u4f7f\u7528\u52d5\u7269\u8840\u6e05\u4f5c\u70ba\u6b63\u5e38\u7d30\u80de\u4ee3\u8b1d\u6240\u9700\u7684\u751f\u9577\u56e0\u5b50\u548c\u71df\u990a\u4f86\u6e90\u3002\u4f46\u662f\uff0c\u4f7f\u7528\u52d5\u7269\u8840\u6e05\uff0c\u5bb9\u6613\u589e\u52a0\u75c5\u6bd2\uff0c\u652f\u539f\u9ad4\u548c\u670a\u75c5\u6bd2\u6c61\u67d3\u7684\u98a8\u96aa\uff1b\u8207\u8840\u6e05\u6536\u96c6\u76f8\u95dc\u7684\u9ad8\u6210\u672c\u548c\u502b\u7406\u554f\u984c\uff1b\u5c0d\u53ef\u80fd\u9084\u542b\u6709\u751f\u9577\u6291\u5236\u5291\u3002\u9ad8\u54c1\u8ceaGrowth factors&cytokines\u7522\u54c1\u5177\u6709\u4ee5\u4e0b\u7279\u9ede\uff1a(A) \u56b4\u683c\u689d\u4ef6\u4e0b\u7684\u9ad8\u7d14\u5ea6\u751f\u7522\u78ba\u4fdd\u8cea\u91cf\u4fdd\u6301\u4e00\u81f4\uff0c\u6bcf\u6279\u6279\u6b21\u7d14\u5ea6\u226595\uff05\u901a\u904eSDS-PAGE. (B) \u5167\u6bd2\u7d20\u6700\u5c0f – Limulus Amebocyte Lysate (LAL) assay\u3002\u4f4e\u65bc1 EU\/ug recombinant protein\u3002(C) \u78ba\u4fdd\u6bcf\u4e00\u6279\u751f\u7269\u6d3b\u6027\u3002\"\"<\/p>\n

\u91cd\u7d44\u4eba\u767d\u8840\u75c5\u6291\u5236\u56e0\u5b50\uff08LIF\uff09<\/strong> (CHO Cell) \u00a0| <\/strong>\u8ca8\u865f<\/strong><\/a><\/span>Z101345<\/a><\/span>\u00a0 \u00a0<\/strong><\/p>\n

LIF\u3001\u5206\u5316\u523a\u6fc0\u56e0\u5b50\u3001D\u56e0\u5b50\u3001\u9ed1\u7d20\u7624\u4f86\u6e90\u7684LPL\u6291\u5236\u5291\u3001MLPLI\u3001INN = Emfilermin\uff0cHILDA\u3001\u91cd\u7d44\u4eba\u767d\u8840\u75c5\u6291\u5236\u56e0\u5b50\uff08\u00a0Leukemia Inhibitory Factor , LIF\uff09<\/p>\n

\u7522\u54c1\u4ecb\u7d39: LIF\u662f\u4ee5\u53ef\u6eb6\u6027(soluble)\u548c\u57fa\u8cea\u7d50\u5408\u5f62\u5f0f(\u00a0matrix-bound forms)\u5b58\u5728\u7684\u591a\u529f\u80fd\u5206\u6ccc\u578b\u7cd6\u86cb\u767d(secreted glycoprotein)\u3002 \u5b83\u986f\u793a\u751f\u7269\u6d3b\u6027(biologic activities )\uff0c\u5f9e\u9aa8\u9ad3\u767d\u8840\u75c5\u7d30\u80de(myeloid leukemic cells )\u5206\u5316\u70ba\u5de8\u566c\u7d30\u80de\u8b5c\u7cfb(macrophage lineage )\uff0c\u5c0d\u9aa8\u4ee3\u8b1d(\u00a0bone metabolism)\uff0c\u708e\u75c7(\u00a0inflammation)\uff0c\u795e\u7d93\u767c\u80b2(neural development)\uff0c\u80da\u80ce\u5f62\u6210(\u00a0embryogenesis)\u548c\u690d\u5165(\u00a0implantation)\u7dad\u6301\u7684\u5f71\u97ff\u3002 \u73fe\u5728\u6e05\u695a\u7684\u662f\uff0cLIF\u5728\u7d50\u69cb\u548c\u4f5c\u7528\u6a5f\u5236\u4e0a\u90fd\u8207\u7d30\u80de\u56e0\u5b50\u767d\u7d30\u80de\u4ecb\u7d20IL-6\u5bb6\u65cf(\u00a0interleukin IL-6 family)\u6709\u95dc\uff0c\u7d30\u80de\u56e0\u5b50\u4e5f\u5305\u62ecIL-11\uff0c\u776b\u72c0\u795e\u7d93\u71df\u990a\u56e0\u5b50(\u00a0ciliary neurotrophic factor)\uff0c\u5236\u7624\u7d20M (\u00a0oncostatin M)\u548c\u5fc3\u808c\u71df\u990a\u56e0\u5b501(cardiotrophin 1)\u3002\u9019\u4e9b\u7d30\u80de\u56e0\u5b50\u7684\u4f5c\u7528\u4ecb\u5c0e \u901a\u904e\u7279\u5b9a\u7684\u7d30\u80de\u8868\u9762\u53d7\u9ad4\u7531\u7368\u7279\u7684\u934a\u548c\u5171\u4eab\u7684\u4fe1\u865f\u8f49\u5c0e\u5b50\u55ae\u5143gp130\u7d44\u6210\u3002<\/p>\n

\"\u76f8\u95dc\u8a66\u5291\"<\/strong>\u00a0aFGF\u91cd\u7d44\u86cb\u767d<\/a>\u00a0|\u00a0IL-2<\/strong>\u91cd\u7d44\u86cb\u767d<\/strong>\u00a0<\/a>\u00a0|\u00a0\u5e79\u7d30\u80de\u56e0\u5b50\uff08KITLG\uff09<\/a>\u00a0|\u00a0\u91cd\u7d44\u4eba\u767d\u8840\u75c5\u6291\u5236\u56e0\u5b50\uff08LIF\uff09<\/a>\u00a0|\u00a0\u91cd\u7d44\u4ebaHuman Noggin (NOG)\u00a0<\/a>\u00a0|\u00a0TNF-a<\/a>\u00a0 |\u00a0BMP2\u00a0<\/a><\/strong><\/span><\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Gene Symbol<\/strong><\/td>\nLIF<\/td>\n<\/tr>\n
Gene ID<\/strong><\/td>\n3976<\/td>\n<\/tr>\n
Accession No.<\/strong><\/td>\nP15018<\/td>\n<\/tr>\n
Source<\/strong><\/td>\nCHO cells<\/td>\n<\/tr>\n
Appearance<\/strong><\/td>\nLyophilized Powder<\/td>\n<\/tr>\n
Molecular Weight<\/strong><\/td>\n20.0 kDa<\/td>\n<\/tr>\n
Endotoxin Level<\/strong><\/td>\n<1.0 EU\/\u03bcg of recombinant protein as determined by the LAL method.<\/td>\n<\/tr>\n
Purity<\/strong><\/td>\n>95% as determined by SDS-PAGE<\/td>\n<\/tr>\n
Bioactivity<\/strong><\/td>\nThe ED(50) was determined by the dose-dependent differentiation of M1 myeloid leukemic cells was found to be in the range of 0.01 ng\/mL.<\/td>\n<\/tr>\n
Formulation<\/strong><\/td>\nRecombinant Leukemia Inhibitory Factor was lyophilized from a 0.2 \u03bcm filtered PBS pH 4.5<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Product<\/strong><\/td>\nSource<\/strong><\/td>\nSize<\/strong><\/td>\nCat. No.<\/strong><\/td>\n<\/tr>\n
Recombinant Human THPO<\/a><\/td>\nHEK293<\/td>\n10 \u00b5g<\/td>\nZ103085<\/td>\n<\/tr>\n
Recombinant Human THPO<\/a><\/td>\nHEK293<\/td>\n100 \u00b5g<\/td>\nZ103087<\/td>\n<\/tr>\n
Recombinant Human LIF<\/a><\/td>\nE. coli<\/em><\/td>\n10 \u00b5g<\/td>\nZ100605<\/td>\n<\/tr>\n
Recombinant Mouse LIF<\/a><\/td>\nE. coli<\/em><\/td>\n10 \u00b5g<\/td>\nZ200195<\/td>\n<\/tr>\n
Recombinant Human SERPINF1<\/a><\/td>\nE. coli<\/em><\/td>\n10 \u00b5g<\/td>\nZ100765<\/td>\n<\/tr>\n
Recombinant Human MIF<\/a><\/td>\nE. coli<\/em><\/td>\n25 \u00b5g<\/td>\nZ100915<\/td>\n<\/tr>\n
Recombinant Mouse LIF<\/a><\/td>\nCHO cells<\/td>\n100 \u00b5g<\/td>\nZ200485<\/td>\n<\/tr>\n
Recombinant Human LIF<\/a><\/td>\nCHO cells<\/td>\n50 \u00b5g<\/td>\nZ101345<\/td>\n<\/tr>\n
Recombinant Human THPO<\/a><\/td>\nE. coli<\/em><\/td>\n10 \u00b5g<\/td>\nZ101515<\/td>\n<\/tr>\n
Recombinant Mouse THPO<\/a><\/td>\nE. coli<\/em><\/td>\n10 \u00b5g<\/td>\nZ200535<\/td>\n<\/tr>\n
Recombinant Mouse LIF<\/a><\/td>\nE. coli<\/em><\/td>\n1.0 mg<\/td>\nZ200199<\/td>\n<\/tr>\n
Recombinant Human MIF<\/a><\/td>\nE. coli<\/em><\/td>\n1.0 mg<\/td>\nZ100919<\/td>\n<\/tr>\n
Recombinant Human LIF<\/a><\/td>\nE. coli<\/em><\/td>\n1.0 mg<\/td>\nZ100609<\/td>\n<\/tr>\n
Recombinant Human SERPINF1<\/a><\/td>\nE. coli<\/em><\/td>\n1.0 mg<\/td>\nZ100769<\/td>\n<\/tr>\n
Recombinant Human THPO<\/a><\/td>\nE. coli<\/em><\/td>\n1.0 mg<\/td>\nZ101519<\/td>\n<\/tr>\n
Recombinant Mouse THPO<\/a><\/td>\nE. coli<\/em><\/td>\n1.0 mg<\/td>\nZ200539<\/td>\n<\/tr>\n
Recombinant Human LIF<\/a><\/td>\nE. coli<\/em><\/td>\n100 \u00b5g<\/td>\nZ100607<\/td>\n<\/tr>\n
Recombinant Human MIF<\/a><\/td>\nE. coli<\/em><\/td>\n100 \u00b5g<\/td>\nZ100917<\/td>\n<\/tr>\n
Recombinant Human SERPINF1<\/a><\/td>\nE. coli<\/em><\/td>\n500 \u00b5g<\/td>\nZ100767<\/td>\n<\/tr>\n
Recombinant Mouse LIF<\/a><\/td>\nE. coli<\/em><\/td>\n100 \u00b5g<\/td>\nZ200197<\/td>\n<\/tr>\n
Recombinant Porcine LIF<\/a><\/td>\nE. coli<\/em><\/td>\n10 \u00b5g<\/td>\nZ400015<\/td>\n<\/tr>\n
Recombinant Porcine LIF<\/a><\/td>\nE. coli<\/em><\/td>\n1.0 mg<\/td>\nZ400019<\/td>\n<\/tr>\n
Recombinant Porcine LIF<\/a><\/td>\nE. coli<\/em><\/td>\n100 \u00b5g<\/td>\nZ400017<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u3010Growth factors&cytokines\u7d30\u80de\u751f\u9577\u56e0\u5b50\u3011 CHO cell\/HEK293\u00a0\/E […]<\/p>\n","protected":false},"author":3,"featured_media":14679,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[139],"tags":[3109],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14683"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=14683"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14683\/revisions"}],"predecessor-version":[{"id":21327,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14683\/revisions\/21327"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/14679"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=14683"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=14683"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=14683"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}