{"id":14678,"date":"2017-10-18T11:49:52","date_gmt":"2017-10-18T03:49:52","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=14678"},"modified":"2022-07-21T10:26:08","modified_gmt":"2022-07-21T02:26:08","slug":"%e5%b9%b9%e7%b4%b0%e8%83%9e%e5%9b%a0%e5%ad%90%ef%bc%88kitlg%ef%bc%89%e7%94%9f%e9%95%b7%e5%9b%a0%e5%ad%90%e5%92%8c%e7%b4%b0%e8%83%9e%e5%9b%a0%e5%ad%90-cho-cell%e8%a1%a8%e7%8f%be%e8%9b%8b%e7%99%bdabm","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=14678","title":{"rendered":"\u5e79\u7d30\u80de\u56e0\u5b50\uff08KITLG\uff09\u751f\u9577\u56e0\u5b50\u548c\u7d30\u80de\u56e0\u5b50 | CHO Cell\u8868\u73fe\u86cb\u767dABM\u8ca8\u865fZ200505"},"content":{"rendered":"

\"ABM\u3010<\/span><\/strong>Growth factors&cytokines<\/strong>\u7d30\u80de\u751f\u9577\u56e0\u5b50<\/strong>\u3011 CHO cell\/<\/strong>HEK293<\/strong> \/E.coli<\/strong>\u8868\u73fe\u7cfb\u7d71<\/strong><\/span><\/span><\/p>\n

Growth factors\u751f\u9577\u56e0\u5b50\u548ccytokines\u7d30\u80de\u56e0\u5b50\uff0c\u662f\u5929\u7136\u5b58\u5728\u7684signaling molecules\u4fe1\u865f\u5206\u5b50\uff0c\u5b83\u5011\u662f\u9ad8\u7b49\u751f\u7269\u7d30\u80de\u4e4b\u9593\u651c\u5e36\u8abf\u7bc0\u4fe1\u865f\u7684\u95dc\u9375\u56e0\u7d20\u3002\u5118\u7ba1\u7d30\u80de\u56e0\u5b50\u4e3b\u8981\u8207immune system housekeeping\u514d\u75ab\u7cfb\u7d71\u76f8\u95dc\uff0c\u751f\u9577\u56e0\u5b50\u5728\u751f\u7269\u9ad4\u7684\u767c\u80b2\u548c\u6210\u719f\u968e\u6bb5\u8abf\u7bc0\u5177\u5ee3\u6cdb\u7684\u904e\u7a0b\uff1b\u4e0d\u53d7\u63a7\u5236\u7684\u751f\u9577\u56e0\u5b50\u6fc0\u6d3b\u901a\u5e38\u8207oncogenesis\u816b\u7624\u767c\u751f\u76f8\u95dc\u3002\u751f\u9577\u56e0\u5b50\u548c\u7d30\u80de\u56e0\u5b50\u5c0d\u65bc\u7d30\u80de\u7684\u8a31\u591a\u529f\u80fd\u662f\u4e0d\u53ef\u7f3a\u5c11\u7684\u3002\u50b3\u7d71\u7684\u7d30\u80de\u751f\u7269\u5b78\u5be6\u9a57\u4f7f\u7528\u52d5\u7269\u8840\u6e05\u4f5c\u70ba\u6b63\u5e38\u7d30\u80de\u4ee3\u8b1d\u6240\u9700\u7684\u751f\u9577\u56e0\u5b50\u548c\u71df\u990a\u4f86\u6e90\u3002\u4f46\u662f\uff0c\u4f7f\u7528\u52d5\u7269\u8840\u6e05\uff0c\u5bb9\u6613\u589e\u52a0\u75c5\u6bd2\uff0c\u652f\u539f\u9ad4\u548c\u670a\u75c5\u6bd2\u6c61\u67d3\u7684\u98a8\u96aa\uff1b\u8207\u8840\u6e05\u6536\u96c6\u76f8\u95dc\u7684\u9ad8\u6210\u672c\u548c\u502b\u7406\u554f\u984c\uff1b\u5c0d\u53ef\u80fd\u9084\u542b\u6709\u751f\u9577\u6291\u5236\u5291\u3002\u9ad8\u54c1\u8ceaGrowth factors&cytokines\u7522\u54c1\u5177\u6709\u4ee5\u4e0b\u7279\u9ede\uff1a(A) \u56b4\u683c\u689d\u4ef6\u4e0b\u7684\u9ad8\u7d14\u5ea6\u751f\u7522\u78ba\u4fdd\u8cea\u91cf\u4fdd\u6301\u4e00\u81f4\uff0c\u6bcf\u6279\u6279\u6b21\u7d14\u5ea6\u226595\uff05\u901a\u904eSDS-PAGE. (B) \u5167\u6bd2\u7d20\u6700\u5c0f – Limulus Amebocyte Lysate (LAL) assay\u3002\u4f4e\u65bc1 EU\/ug recombinant protein\u3002(C) \u78ba\u4fdd\u6bcf\u4e00\u6279\u751f\u7269\u6d3b\u6027\u3002<\/p>\n

\"\"<\/p>\n

\u5e79\u7d30\u80de\u56e0\u5b50\uff08KITLG\uff09\u751f\u9577\u56e0\u5b50\u548c\u7d30\u80de\u56e0\u5b50 (CHO Cell) \u00a0| <\/strong>\u8ca8\u865f<\/strong>Z200505 <\/strong><\/a><\/span><\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> \u5e79\u7d30\u80de\u56e0\u5b50\uff08KITLG\uff09\u751f\u9577\u56e0\u5b50\u548c\u7d30\u80de\u56e0\u5b50\uff0c<\/strong>\u540c\u7fa9\u5b57<\/strong>SCF, Kit ligand, c-Kit ligand, Hematopoietic growth factor KL, Mast cell growth factor, MGF, Steel factor\u3002SCF\u662f\u7531\u6210\u7e96\u7dad\u7d30\u80de\u548c\u5176\u4ed6\u7d30\u80de\u985e\u578b\u5408\u6210\u7684\u57fa\u8cea\u7d30\u80de\u4f86\u6e90\u7684\u7d30\u80de\u56e0\u5b50\u3002SCF\u4fc3\u9032\u591a\u80fd\u5e79\u7d30\u80de\u6c34\u5e73\u7684\u7d30\u80de\u589e\u6b96\u548c\u65e9\u671f\u5206\u5316\u3002\u5df2\u7d93\u63d0\u51faSCF\u5c0d\u65bc\u5404\u7a2e\u9020\u8840\u8b5c\u7cfb\u7684\u6700\u4f73\u751f\u7522\u662f\u5fc5\u4e0d\u53ef\u5c11\u7684\uff0c\u4e3b\u8981\u662f\u56e0\u70ba\u5176\u8207\u5176\u5b83\u7d30\u80de\u56e0\u5b50\u5171\u540c\u523a\u6fc0\u6642\u80fd\u5920\u9810\u9632\u7d30\u80de\u51cb\u4ea1\u3002 \u7a31\u70baSCFR\uff08CD117\uff09\u7684SCF\u53d7\u9ad4\u662f\u6307\u5b9a\u70baKIT\u7684\u81f4\u764c\u57fa\u56e0\u3002 SCF\u7684\u751f\u7269\u6d3b\u6027\u901a\u904e\u96c6\u843d\u523a\u6fc0\u56e0\u5b50GM-CSF\u548cG-CSF\u4ee5\u53caIL-7\uff0cEpo\u7b49\u4e00\u4e9b\u751f\u9577\u548c\u5206\u5316\u56e0\u5b50\u76f8\u4e92\u5354\u540c\u3002 \u7d50\u5408IL-7 SCF\u523a\u6fc0\u524dB\u7d30\u80de\u7684\u589e\u6b96\u3002 SCF\u4e5f\u662f\u7d30\u80de\u7684\u6709\u6548\u5316\u5b78\u5f15\u8a98\u7269\uff08\u53c3\u898b\uff1a\u8da8\u5316\u6027\uff09\uff0c\u4f8b\u5982\u8868\u9054\u8a66\u5291\u76d2\u53d7\u9ad4\u7684\u80a5\u5927\u7d30\u80de\u3002 \u9019\u4e9b\u7d30\u80de\u4e2d\u5c0dSCF\u7684\u4e00\u500b\u53cd\u61c9\u662f\u7d30\u80de\u9aa8\u67b6\u7684\u808c\u52d5\u86cb\u767d\u7d72\u7684\u7279\u5fb5\u91cd\u6392\u3002<\/p>\n

\"\u76f8\u95dc\u8a66\u5291\"<\/strong>\u00a0aFGF\u91cd\u7d44\u86cb\u767d<\/a>\u00a0|\u00a0IL-2<\/strong>\u91cd\u7d44\u86cb\u767d<\/strong>\u00a0<\/a>\u00a0|\u00a0\u5e79\u7d30\u80de\u56e0\u5b50\uff08KITLG\uff09<\/a>\u00a0|\u00a0\u91cd\u7d44\u4eba\u767d\u8840\u75c5\u6291\u5236\u56e0\u5b50\uff08LIF\uff09<\/a>\u00a0|\u00a0\u91cd\u7d44\u4ebaHuman Noggin (NOG)\u00a0<\/a>\u00a0|\u00a0TNF-a<\/a>\u00a0 |\u00a0BMP2\u00a0<\/a><\/strong><\/span><\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Gene Symbol<\/strong><\/td>\nKITLG<\/td>\n<\/tr>\n
Gene ID<\/strong><\/td>\n17311<\/td>\n<\/tr>\n
Accession No.<\/strong><\/td>\nP20826<\/td>\n<\/tr>\n
Source<\/strong><\/td>\nCHO cells<\/td>\n<\/tr>\n
Appearance<\/strong><\/td>\nLyophilized Powder<\/td>\n<\/tr>\n
Molecular Weight<\/strong><\/td>\n18.0 kDa<\/td>\n<\/tr>\n
Endotoxin Level<\/strong><\/td>\n<1.0 EU\/\u03bcg of recombinant protein as determined by the LAL method.<\/td>\n<\/tr>\n
Purity<\/strong><\/td>\n>95% as determined by SDS-PAGE<\/td>\n<\/tr>\n
Bioactivity<\/strong><\/td>\nThe ED(50) was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells to be < 2.0 ng\/mL, corresponding to a specific activity of > 5.0 x 10^5 units\/mg.<\/td>\n<\/tr>\n
Formulation<\/strong><\/td>\nRecombinant Stem cell factor was lyophilized from a 0.2 \u03bcm filtered solution in 20mM Tris, 5% trehalose, pH 7.5.<\/td>\n<\/tr>\n
Reconstitution<\/strong><\/td>\nA quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg\/mL. This solution can then be diluted into other buffers.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Product<\/strong><\/td>\nSource<\/strong><\/td>\nSize<\/strong><\/td>\nCat. No.<\/strong><\/td>\n<\/tr>\n
Recombinant Human Stem Cell Factor (KITLG)<\/a><\/td>\nHEK293<\/td>\n10 \u00b5g<\/td>\nZ103035<\/td>\n<\/tr>\n
Recombinant Human Stem Cell Factor (KITLG)<\/a><\/td>\nHEK293<\/td>\n100 \u00b5g<\/td>\nZ103037<\/td>\n<\/tr>\n
Recombinant Human Stem Cell Factor (KITLG)<\/a><\/td>\nE. coli<\/em><\/td>\n10 \u00b5g<\/td>\nZ100815<\/td>\n<\/tr>\n
Recombinant Mouse Stem Cell Factor (KITLG)<\/a><\/td>\nCHO cells<\/td>\n100 \u00b5g<\/td>\nZ200505<\/td>\n<\/tr>\n
Recombinant Human Stem Cell Factor (KITLG)<\/a><\/td>\nCHO cells<\/td>\n25 \u00b5g<\/td>\nZ101375<\/td>\n<\/tr>\n
Recombinant Mouse Stem Cell Factor (KITLG)<\/a><\/td>\nE. coli<\/em><\/td>\n10 \u00b5g<\/td>\nZ200595<\/td>\n<\/tr>\n
Recombinant Human Stem Cell Factor (KITLG)<\/a><\/td>\nE. coli<\/em><\/td>\n1.0 mg<\/td>\nZ100819<\/td>\n<\/tr>\n
Recombinant Mouse Stem Cell Factor (KITLG)<\/a><\/td>\nE. coli<\/em><\/td>\n1.0 mg<\/td>\nZ200599<\/td>\n<\/tr>\n
Recombinant Human Stem Cell Factor (KITLG)<\/a><\/td>\nE. coli<\/em><\/td>\n100 \u00b5g<\/td>\nZ100817<\/td>\n<\/tr>\n
Recombinant Mouse Stem Cell Factor (KITLG)<\/a><\/td>\nE. coli<\/em><\/td>\n100 \u00b5g<\/td>\nZ200597<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u3010Growth factors&cytokines\u7d30\u80de\u751f\u9577\u56e0\u5b50\u3011 CHO cell\/HEK293 \/E […]<\/p>\n","protected":false},"author":3,"featured_media":14679,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[139],"tags":[3110],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14678"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=14678"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14678\/revisions"}],"predecessor-version":[{"id":14718,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14678\/revisions\/14718"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/14679"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=14678"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=14678"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=14678"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}