{"id":14485,"date":"2017-10-13T22:10:25","date_gmt":"2017-10-13T14:10:25","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=14485"},"modified":"2022-07-21T10:29:15","modified_gmt":"2022-07-21T02:29:15","slug":"%e5%a4%a7%e8%85%b8%e6%a1%bf%e8%8f%8co157-captivate%e6%8a%97%e9%ab%94%e5%85%8d%e7%96%ab%e7%a3%81%e6%80%a7%e5%88%86%e9%9b%a2%e6%b3%95-%e8%b2%a8%e8%99%9fcap001","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=14485","title":{"rendered":"\u5927\u8178\u687f\u83ccO157 | Captivate\u2122\u6297\u9ad4\u514d\u75ab\u78c1\u6027\u5206\u96e2\u6cd5, \u8ca8\u865fCAP001"},"content":{"rendered":"

\u5fae\u751f\u7269\u7d14\u5316\uff0dCaptivate\u2122\u6297\u9ad4\u514d\u75ab\u78c1\u6027\u5206\u96e2\u6cd5<\/span><\/p>\n

Captivate\u2122\u662f\u7528\u65bc\u5fae\u751f\u7269\u7684\u7279\u7570\u6027\u514d\u75ab\u78c1\u6027\u5206\u96e2\uff08ImmunoMagnetic Separation, IMS\uff09\u7684\u4e00\u7cfb\u5217\u5546\u54c1\uff1b\u6297\u9ad4\u5171\u50f9\u9375\u7d50\u9806\u78c1\u6027\u4e4b\u514d\u75ab\u78c1\u6027\u5206\u96e2\u73e0\uff0c\u53ef\u5c08\u4e00\u6027\u88dc\u6349\u76ee\u6a19\u5fae\u751f\u7269\uff0c\u7d50\u5408\u78c1\u6027\u7279\u6027\u53ef\u5feb\u901f\u5206\u96e2\u5f85\u6aa2\u6e2c\u4e4b\u5fae\u751f\u7269\u3002\u6297\u9ad4\u9ad8\u5ea6\u7279\u7570\u6027\u4e4b\u8a2d\u8a08\u61c9\u7528\u65bc\u5f9e\u5bcc\u96c6\u57f9\u990a\u7269\u4e2d\uff0c\u5c08\u4e00\u6027\u5206\u96e2\u51fa\u76ee\u6a19\u7d30\u83cc\u3002 IMS\u6bd4\u50b3\u7d71\u65b9\u5f0f\uff0c\u66f4\u63d0\u9ad8\u4e86\u7d30\u83cc\u5206\u96e2\u65b9\u6cd5\u4e4b\u9748\u654f\u5ea6\uff0c\u5728\u5927\u591a\u6578\u60c5\u6cc1\u4e0b\uff0c24\u5c0f\u6642\u5373\u53ef\u5b8c\u6210\u5206\u96e2\u3002<\/p>\n

\"Captivate<\/p>\n

Captivate\u2122 O157<\/strong>\u6297\u9ad4\u514d\u75ab\u78c1\u6027\u5206\u96e2 | \u8ca8\u865fCAP001<\/strong><\/a><\/span><\/p>\n

\u7522\u54c1\u4ecb\u7d39\uff1a<\/strong>Captivate\u2122 O157\u70ba\u6fc3\u7e2e\u548c\u5206\u96e2\u5927\u8178\u687f\u83ccO157\u7684\u514d\u75ab\u78c1\u6027\u5206\u96e2\u73e0\u3002Captivate\u2122O157\u5857\u8986\u6709\u7279\u7570\u6027\u6297\u9ad4\u7684\u53ef\u78c1\u5316\u9846\u7c92\uff0c\u7528\u65bc\u5f9e\u98df\u54c1\uff0c\u52d5\u7269\u98fc\u6599\uff0c\u98f2\u6599\uff0c\u85e5\u7269\u6216\u74b0\u5883\u6a23\u54c1\u4e2d\u6fc3\u7e2e\u548c\u5206\u96e2\u5927\u8178\u687f\u83ccO157\u3002\u7522\u54c1\u7279\u6027<\/strong>:<\/strong> \u4f7f\u7528\u6fc3\u5ea6\u70ba5mg \/ ml, \u6297\u9ad4\u5177\u6709\u9846\u7c92\u5857\u8986\u9ad8\u89aa\u5408\u529b\uff0c\u53ef\u9ad8\u89aa\u548c\u529b\u7d14\u5316\u548c\u5438\u6536\u7d30\u80de\u8868\u9762\u6297\u539f, \u8207\u5927\u8178\u687f\u83ccO157\u5177\u7279\u7570\u6027\u53cd\u61c9\u3002\u5e73\u5747\u5927\u5c0f\u70ba2.5\u03bcm\uff08\u5178\u578b\u7bc4\u570d1-8\u03bcm\uff09, \u8caf\u5b582-8oC\u3002\u8caf\u5b58\u6eb6\u6db2: \u78f7\u9178\u9e7d\u7de9\u885d\u9e7d\u6c34\uff08PBS\uff09\u3001\u725b\u8840\u6e05\u767d\u86cb\u767d\uff08BSA\uff091\uff05\u3001\u758a\u6c2e\u5316\u92090.09\uff05\u3002<\/p>\n

\u5927\u8178\u687f\u83ccO157\u7684\u5206\u96e2<\/strong>\u65b9\u6cd5<\/strong>: <\/strong><\/p>\n

1.\u5c0725g\u6a23\u54c1\u7a31\u91cd\u5230\u4e00\u500b\u53e3\u888b\u4e2d\uff0c\u7136\u5f8c\u52a0\u5165225ml enrichment broth\u4e26\u52fb\u6f3f\u3002<\/p>\n

2.\u572842\u2103\u5b75\u80b26\u5c0f\u6642\u3002<\/p>\n

3.\u6fc3\u7e2e\u6fc3\u7e2e\u57f9\u990a\u7269\uff0c\u7136\u5f8c\u653e\u7f6e5\u5206\u9418\u3002<\/p>\n

4.\u5c07CaptivateTM O157\u9846\u7c92\u5145\u5206\u6df7\u5408\uff0c\u4e26\u5c0720\u03bclCaptivateTM O157\u9846\u7c92\u52a0\u5165\u5408\u9069\u7684\u7ba1\u4e2d\uff08\u7d041.5-2.5ml\u9ad4\u7a4d\uff09\u3002<\/p>\n

5.\u52a0\u5165\u542b\u6709CaptivateTM O157\u9846\u7c92\u76841ml\u6fc3\u7e2e\u57f9\u990a\u7269\uff08\u6ce8\u610f\u907f\u514d\u98df\u7269\u6b98\u6e23\u7684\u79fb\u6db2\uff09\u3002<\/p>\n

6.\u5c07\u7ba1\u5b50\u84cb\u7dca\u4e26\u5c07\u6df7\u5408\u7269\u5728\u5ba4\u6eab\u4e0b\u6df7\u540830\u5206\u9418\u3002<\/p>\n

7.\u5c07\u7ba1\u63d2\u5165\u78c1\u529b\u5206\u96e2\u56683\u5206\u9418\uff0c\u4ee5\u5206\u96e2\u9846\u7c92\u3002<\/p>\n

8.\u5c0f\u5fc3\u5730\u5f9e\u7ba1\u4e2d\u5438\u51fa\u4e0a\u6e05\u6db2\uff0c\u4e0d\u8981\u53bb\u9664\u9846\u7c92\u4e26\u66f4\u63db\u84cb\u5b50\u3002<\/p>\n

9.\u5f9e\u6a5f\u67b6\u4e0a\u53d6\u4e0b\u78c1\u9435\uff08\u6216\u5f9e\u78c1\u9078\u6a5f\u67b6\u4e0a\u53d6\u4e0b\u7ba1\u5b50\uff09\uff0c\u52a0\u51651ml Wash\uff0c\u5c07\u53cd\u61c9\u7269\u91cd\u65b0\u61f8\u6d6e\u5e7e\u6b21\u3002<\/p>\n

10.\u91cd\u8907\u6b65\u9a5f7-9\u6d17\u6ecc\u9846\u7c92\u5169\u6b21\u3002\u6700\u5f8c\u91cd\u61f8\u65bc100\u03bcl\u6d17\u6ecc\u6db2\u4e2d\u3002<\/p>\n

11.\u53d6\u51fa\u8907\u5408\u7684\u61f8\u6d6e\u9846\u7c92\u4e26\u8f49\u79fb\u5230CT-SMAC\uff0cSMAC\u548c\/\u6216SMAC-BCIG\u677f\u4e0a\u3002\u9023\u7e8c\/\u64f4\u6563\u521d\u59cb\u63a5\u7a2e\u7269\u4ee5\u5be6\u73fe\u55ae\u500b\u83cc\u843d\u3002\u6aa2\u67e5\u5178\u578b\u7684typical sorbitol-negative\u5c71\u68a8\u7cd6\u9187\u9670\u6027\u83cc\u843d\u7684\u751f\u9577\u3002<\/p>\n

\"\u76f8\u95dc\u8a66\u5291\"\u00a0<\/span><\/strong>\u86cb\u767d\u8156(peptone)<\/a><\/span> | \u725b\u8089\u62bd\u51fa\u7269(beef extract)<\/a> <\/span>| \u9175\u6bcd\u62bd\u51fa\u7269(yeast extract)<\/a><\/span> | Agar No 1 Bacteriological\u00a0<\/a><\/span>\u00a0|\u00a0<\/a><\/span><\/strong>MRS agar<\/a><\/span> | MRS Broth<\/a>\u00a0|\u00a0\u8461\u8404\ufa03Glucose<\/a>\u00a0<\/a><\/strong>(Dextrose)<\/a>\u00a0|\u00a0\u4e73\u7cd6Lactose<\/a><\/span><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u5fae\u751f\u7269\u7d14\u5316\uff0dCaptivate\u2122\u6297\u9ad4\u514d\u75ab\u78c1\u6027\u5206\u96e2\u6cd5 Captivate\u2122\u662f\u7528\u65bc\u5fae\u751f\u7269\u7684\u7279\u7570\u6027\u514d\u75ab\u78c1\u6027\u5206\u96e2\uff08Im […]<\/p>\n","protected":false},"author":3,"featured_media":14486,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[511],"tags":[3139,3140],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14485"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=14485"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14485\/revisions"}],"predecessor-version":[{"id":14491,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14485\/revisions\/14491"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/14486"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=14485"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=14485"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=14485"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}