{"id":14110,"date":"2017-09-21T14:22:42","date_gmt":"2017-09-21T06:22:42","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=14110"},"modified":"2022-07-21T10:35:00","modified_gmt":"2022-07-21T02:35:00","slug":"%e6%b0%b8%e7%94%9f%e5%8c%96%e5%a4%a7%e9%bc%a0%e8%a6%96%e7%b6%b2%e8%86%9c%e5%89%8d%e9%ab%94%e7%b4%b0%e8%83%9e-immortalized-rat-retinal-precursor-cells-r28-%e8%b2%a8%e8%99%9ft0282","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=14110","title":{"rendered":"\u6c38\u751f\u5316\u5927\u9f20\u8996\u7db2\u819c\u524d\u9ad4\u7d30\u80de | Immortalized Rat Retinal Precursor Cells (R28) \u8ca8\u865fT0282"},"content":{"rendered":"<p><strong>A<\/strong><strong>bmgood<\/strong><strong>\u4e0d<\/strong><strong>\u673d<\/strong><strong>\u5316\u7cfb\u5217\u5546\u54c1<\/strong><strong> (immortalized cell)<\/strong><\/p>\n<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=14110\" target=\"_blank\"><strong>\u6c38\u751f\u5316\u5927\u9f20\u8996\u7db2\u819c\u524d\u9ad4\u7d30\u80de\u00a0 |\u00a0<\/strong><strong>\u8ca8\u865f<\/strong>T0282<\/a><\/span><\/p>\n<p>Immortalized Rat Retinal Precursor Cells (R28)<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/www.abmgood.com\/admin\/IHCpics\/T0282-1.jpg\" alt=\"\" \/><\/p>\n<p>\u6c38\u751f\u5316\u5927\u9f20\u8996\u7db2\u819c\u524d\u9ad4\u7d30\u80de\uff08R28\uff09\u884d\u751f\u81ea\u7528\u817a\u75c5\u6bd2\u768412S E1A\u57fa\u56e0\u6c38\u751f\u5316\u7684\u51fa\u751f\u5f8c\u7b2c6\u5929Sprague-Dawley\u5927\u9f20\u8996\u7db2\u819c\u7d44\u7e54\u3002\u7814\u7a76\u8868\u660e\uff0c\u8a72\u7d30\u80de\u7cfb\u5177\u6709\u53ef\u4ee5\u5c0d\u795e\u7d93\u905e\u8cea\u523a\u6fc0\uff08\u5373\u591a\u5df4\u80fa\uff0c5-\u7fa5\u8272\u80fa\uff0c\u7518\u6c28\u9178\u548c\u4e59\u9170\u81bd\u9e7c\uff09\u8d77\u53cd\u61c9\u7684\u8996\u7db2\u819c\u795e\u7d93\u905e\u8cea\u53d7\u9ad4\uff0c\u4e9e\u7fa4\u5c0dGluR1,2\u548c3\uff0cN-\u7532\u57fa-D-\u5929\u51ac\u6c28\u9178\uff08NMDA\uff09\u5177\u6709\u514d\u75ab\u53cd\u61c9\u6027\uff0c \u03b3-\u6c28\u57fa\u4e01\u9178a\uff08GABAa\uff09\u3002\u6b64\u5916\uff0c\u901a\u904e\u6539\u8b8a\u57f9\u990a\u57fa\u689d\u4ef6\u53ef\u4ee5\u4fc3\u9032\u795e\u7d93\u7a81\u751f\u9577\u3002\u9019\u7a2e\u6c38\u751f\u5316\u5927\u9f20\u8996\u7db2\u819c\u524d\u9ad4\u7d30\u80de\u7cfb\u7684\u7570\u8cea\u6027\u63d0\u4f9b\u4e86\u591a\u7a2e\u7d30\u80de\u985e\u578b\uff0c\u5176\u4e2d\u66f4\u5bc6\u5207\u5730\u6a21\u64ec\u8996\u7db2\u819c\u5916\u690d\u9ad4\u4e26\u63d0\u4f9b\u5728\u6d89\u53ca\u8996\u7db2\u819c\u529f\u80fd\u7684\u7814\u7a76\u4e2d\u6709\u7528\u7684\u5206\u5316\u6f5b\u80fd\u3002\u6700\u8fd1\u516c\u4f48\u4e86R28\u7d30\u80de\u7684\u5fae\u9663\u5217\u6578\u64da\u96c6\uff0c\u63cf\u8ff0\u4e868799\u500b\u57fa\u56e0\u548cEST\u7684\u5b58\u5728\u548c\u4e0d\u5b58\u5728\uff0c\u9019\u4e9b\u57fa\u56e0\u548cEST\u53ef\u80fd\u8207\u4f7f\u7528\u8a72\u7d30\u80de\u7cfb\u7528\u65bc\u7279\u5b9a\u5206\u5b50\u5206\u6790\u7684\u7814\u7a76\u8005\u6709\u95dc\u3002<\/p>\n<p><strong>\u6c38\u751f\u5316\u5927\u9f20\u8996\u7db2\u819c\u524d\u9ad4\u7d30\u80de\uff08R28\uff09<\/strong> \u7d30\u80de\u7279\u6027: Adherent \/ Polygonal | retroviruses carrying 12S E1A gene | \u4f86\u6e90Retinal Tissue \u3002\u7d30\u80de\u57f9\u990a\u689d\u4ef6: Recommended split ratio of 1:2 to 1:3\u3002completed growth medium, add the following components to the base medium: 10% bovine serum (ThermoFisher) and Penicillin\/Streptomycin (G255). Atmosphere: air: 95%, CO\u2082: 5%; Temperature: 37.0\u00b0C. To promote neurite growth, culture the cells in DMEM with 10% bovine serum (ThermoFisher) and 250 M pCPT-cyclic AMP.<\/p>\n<table width=\"100%\">\n<tbody>\n<tr>\n<td><strong>BioSafety Level<\/strong><\/td>\n<td>II<\/td>\n<\/tr>\n<tr>\n<td><strong>Organism<\/strong><\/td>\n<td>Postnatal Day 6 Sprague-Dawley Rat<\/td>\n<\/tr>\n<tr>\n<td><strong>Source Organ<\/strong><\/td>\n<td>Retinal Tissue<\/td>\n<\/tr>\n<tr>\n<td><strong>Growth Properties<\/strong><\/td>\n<td>Adherent<\/td>\n<\/tr>\n<tr>\n<td><strong>Population Doubling<\/strong><\/td>\n<td>39.5 hours<\/td>\n<\/tr>\n<tr>\n<td><strong>Recommended Seeding Density<\/strong><\/td>\n<td>Recommended split ratio of 1:2 to 1:3<\/td>\n<\/tr>\n<tr>\n<td><strong>Markers<\/strong><\/td>\n<td>IRBP, S-antigen<\/td>\n<\/tr>\n<tr>\n<td><strong>Applications<\/strong><\/td>\n<td>For Research Use Only<\/td>\n<\/tr>\n<tr>\n<td><strong>Immortalization Method<\/strong><\/td>\n<td>Serial passaging and transduction with retroviruses carrying 12S E1A gene<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><a href=\"http:\/\/biopioneer.com.tw\/?p=5545\"><u><strong><img decoding=\"async\" loading=\"lazy\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2017\/09\/tag6-e1505358740644-300x106.jpg\" alt=\"\u76f8\u95dc\u8a66\u5291\" width=\"80\" height=\"28\" \/><\/strong>\u00a0<\/u><span style=\"color: #3366ff;\">\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c<\/span><\/a><span style=\"color: #3366ff;\">\u00a0<strong>|<\/strong>\u00a0<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=5582\">DAPI\u67d3\u6838<\/a>\u00a0<strong>|<\/strong>\u00a0<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=5564\">\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291<\/a>\u00a0<strong>|<\/strong>\u00a0<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=5567\">\u6c34\u6d74\u69fd\u6291\u83cc\u5291<\/a>\u00a0<strong>|<\/strong>\u00a0<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=5575\">\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49\u7cfb\u5217\u5546\u54c1<\/a><\/span><\/p>\n<p><strong>Abmgood<\/strong><strong>\u4e0d\u673d\u5316\u7cfb\u5217\u5546\u54c1<\/strong>\u00a0<strong>(immortalized reagenet)<\/strong>\u00a0Ready-to-use<\/p>\n<p>Abmgood\u516c\u53f8\u65bc\u4eba\u985e\u8207\u8001\u9f20\u4e0d\u673d\u4e4b\u7d30\u80de\u682a\u4e0a\uff0c\u5177\u6709\u5e7e\u5341\u5e74\u7684\u5be6\u9a57\u7d93\u9a57\uff1b\u63d0\u4f9b\u4e00\u7cfb\u5217\u4e0d\u673d (immortalized)\u5316\u4e4b\u7d30\u80de\u682a\u3001\u53ca\u4e0d\u673d\u5316\u4e4b\u8a66\u5291\u5546\u54c1\u3002ready-to-use\u5b9a\u91cf\u597d\u4e4bretrovirus\u3001lentiviru\u3001 adenovirus\uff0c\u53ef\u76f4\u63a5\u52a0\u65bc\u7d30\u80de\u4e2d\u9032\u884c\u611f\u67d3\u8868\u73fe\uff1b\u75c5\u6bd2\u6db2\u5305\u62ec\u6709SV40\u91cd\u7d44\u75c5\u6bd2\u3001EBV\u91cd\u7d44\u75c5\u6bd2\u3001hTERT\u75c5\u6bd2\u6db2\u3001Myc\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001p53\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001Rb\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001Ras\u57fa\u56e0\u75c5\u6bd2\u6db2\u3002\u63d0\u4f9b106\u53ca109\u5169\u7a2e\u5305\u88dd\u529b\u50f9\u4e4b\u75c5\u6bd2\u6db2\uff1b\u4ea6\u63d0\u4f9b\u4e00\u7cfb\u5217\u75c5\u6bd2\u529b\u50f9\u6fc3\u7e2e\u8207\u5b9a\u91cf\u8a66\u5291\u5546\u54c1\u3002<\/p>\n<p>\u7d30\u80de\u4e0d\u673d\u5316\u7684\u5de5\u5177\u4e2d\uff0cSV40 T antigen\u662f\u6700\u7c21\u55ae\u4e14\u6700\u5e38\u7528\u4f86\u9020\u6210\u4e0d\u673d\u5316\u7d30\u80de\u751f\u6210\u7684\u65b9\u6cd5\u3002\u591a\u6578\u7684\u75c5\u6bd2\u57fa\u56e0\u548c\u6291\u764c\u57fa\u56e0\uff08p53\u3001Rb\u7b49\uff09\u7684\u4e0d\u6d3b\u5316\u76f8\u95dc\uff0c\u6703\u8a98\u5c0e\u7d30\u80de\u8655\u65bcreplicative senescence\u7684\u968e\u6bb5\u3002\u76ee\u524d\u4e5f\u6709\u7814\u7a76\u986f\u793a\u88ab\u611f\u67d3\u7684\u7d30\u80de\uff0cSV40 T anbtigen\u6703\u8a98\u5c0e\u7aef\u7c92\u9176\uff08Telomerase\uff09\u7684\u6d3b\u6027\u3002\u8207\u7aef\u7c92\uff08telomere\uff09\u9577\u5ea6\u76f8\u95dc\u7684Telomerase Reverse Transcriptase protein (TERT)\u662f\u4e00\u7a2e\u4e0d\u673d\u7d30\u80de\u7684\u7814\u7a76\u61c9\u7528\u3002\u5e73\u5e38\u7d30\u80de\u4e2d\uff0chTERT\u662f\u8655\u65bc\u4e0d\u6d3b\u5316\u614b\uff0c\u5fc5\u8981\u6642\u6703\u9032\u884c\u6d3b\u5316\u4fdd\u6301telomere\u7684\u9577\u5ea6\uff0c\u907f\u514d\u7d30\u80de\u767c\u751freplicative senescence\u7684\u73fe\u8c61\u3002\u67d0\u4e9b\u7d30\u80dehTERT \u904e\u5ea6\u8868\u73fe\uff08over-expression\uff09\u5c0d\u65bc\u8a98\u767c\u7d30\u80de\u4e0d\u673d\u5316\u662f\u7121\u6548\u7684\uff08\u5982\u521d\u4ee3\u4e0a\u76ae\u7d30\u80de\uff09\uff0c\u53cd\u800c\u6703\u9020\u6210\u7d30\u80de\u6b7b\u4ea1\uff1b\u540c\u6642\u8868\u73fehTERT catalytic subunit\u4ee5\u53cap53\u6216RB siRNA\u5728\u4eba\u985e\u521d\u4ee3\u5375\u5de2\u4e0a\u76ae\u7d30\u80de\u6703\u9020\u6210\u7d30\u80de\u4e0d\u673d\u5316\u3002\u904e\u5ea6\u8868\u73feRas\u6216Myc T58A mutants\u4e5f\u6709\u521d\u4ee3\u7d30\u80de\u4e0d\u673d\u5316\u7684\u73fe\u8c61\u7522\u751f\u3002<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Abmgood\u4e0d\u673d\u5316\u7cfb\u5217\u5546\u54c1 (immortalized cell) \u6c38\u751f\u5316\u5927\u9f20\u8996\u7db2\u819c\u524d\u9ad4\u7d30\u80de\u00a0 |\u00a0\u8ca8\u865fT0 [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":11653,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[453],"tags":[3189],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14110"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=14110"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14110\/revisions"}],"predecessor-version":[{"id":14113,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14110\/revisions\/14113"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/11653"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=14110"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=14110"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=14110"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}