{"id":14091,"date":"2017-09-21T12:45:38","date_gmt":"2017-09-21T04:45:38","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=14091"},"modified":"2023-03-17T16:36:31","modified_gmt":"2023-03-17T08:36:31","slug":"%e8%84%82%e8%b3%aa%e9%81%8e%e6%b0%a7%e5%8c%96%ef%bc%88mda%ef%bc%89%e6%af%94%e8%89%b2%e8%9e%a2%e5%85%89%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92-lipid-peroxidation-mda-colorimetricfluorometri","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=14091","title":{"rendered":"\u4e19\u4e8c\u919b\u8102\u8cea\u904e\u6c27\u5316\uff08MDA) \u6aa2\u6e2c\uff08TBA\u6cd5\uff09 | Lipid Peroxidation (MDA) Colorimetric\/Fluorometric Assay Kit\u8ca8\u865fK739 (ABCAM ab118970)"},"content":{"rendered":"

\u8102\u8cea\u904e\u6c27\u5316\uff08MDA\uff09\u6bd4\u8272\/\u87a2\u5149\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/strong>\u00a0|\u00a0<\/strong>\u8ca8\u865f<\/strong>K739<\/a>\u00a0(ABCAM ab118970)<\/span><\/p>\n

Lipid Peroxidation (MDA) Colorimetric\/Fluorometric Assay Kit<\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong>\u8102\u8cea\u904e\u6c27\u5316(\u00a0lipid peroxidation )\u7684\u5b9a\u91cf\uff0c\u5c0d\u65bc\u8a55\u4f30\u75c5\u7406\u751f\u7406\u904e\u7a0b\u4e2d\u7684\u6c27\u5316Stress (oxidative stress ) \u81f3\u95dc\u91cd\u8981\uff0c\u6c27\u5316\u58d3\u529b\u7684\u751f\u5316\u6aa2\u9a57\u3002 \u8102\u8cea\u904e\u6c27\u5316 (Lipid peroxidation) \u5f62\u6210\u4e19\u4e8c\u919b\uff08Malondialdehyde, MDA\uff09\u548c4-\u7fa5\u57fa\u58ec\u70ef\u919b\uff084-hydroxynonenal , 4-HNE\uff09\uff0c\u4f5c\u70ba\u5929\u7136\u96d9\u7522\u7269\u3002\u00a0\u4e19\u4e8c\u919b(Malondialdehyde,MDA)\u662f\u4e00\u7a2e\u751f\u7269\u9ad4\u8102\u8cea\u6c27\u5316\u7684\u5929\u7136\u7522\u7269\u3002 \u6e2c\u91cf\u8102\u8cea\u904e\u6c27\u5316\u7684\u6700\u7d42\u7522\u7269\uff0c\u662f\u6700\u5ee3\u6cdb\u63a5\u53d7\u7684\u6c27\u5316\u640d\u50b7(oxidative damage) \u6e2c\u5b9a\u4e4b\u4e00\u3002 BioVision\u7684\u8102\u8cea\u904e\u6c27\u5316\u6e2c\u5b9a\u8a66\u5291\u76d2(Lipid Peroxidation Assay Kit , K739) \u70ba\u5404\u7a2e\u6a23\u54c1\u4e2dMDA\u7684\u654f\u611f\u6aa2\u6e2c\u63d0\u4f9b\u4e86\u4fbf\u5229\u7684\u5de5\u5177\u3002 \u6a23\u54c1\u4e2d\u7684MDA\u8207\u786b\u4ee3\u5df4\u6bd4\u59a5\u9178\uff08Thiobarbituric Acid, TBA\uff09\u53cd\u61c9\u4ee5\u7522\u751fMDA-TBA\u52a0\u5408\u7269\u3002 \u53ef\u4ee5\u6bd4\u8272\uff08\u03bb= 532nm\uff09\u6216\u87a2\u5149\u6e2c\u91cf\uff08Ex \/ Em = 532 \/ 553nm\uff09\u5bb9\u6613\u5730\u91cf\u5316MDA-TBA\u52a0\u5408\u7269\u3002 \u8a72\u6e2c\u5b9a\u6aa2\u6e2cMDA\u6c34\u5e73\u4f4e\u81f31 nmol \/\u5b54\u6bd4\u8272\u6cd5\u548c0.1 nmol \/\u5b54\u87a2\u5149\u6e2c\u5b9a\u3002<\/p>\n

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 <\/p>\n\n\n\n\n\n\n\n\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (532 nm) or Fluorescence (Ex\/Em 532\/553 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nAll<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nThe kit detects MDA levels as low as 1 nmol\/well colorimetrically and 0.1 nmol\/well fluorometrically.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure
\n\u2022 Fast and convenient
\n\u2022 Sensitive assay for measuring lipid peroxidation (LPA) in a wide range of samples<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 MDA Lysis Buffer
\n\u2022 Phosphotungstic Acid Solution
\n\u2022 BHT (100X)
\n\u2022 TBA
\n\u2022 MDA Standard (4.17 M)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\u66f4\u591a\u6c27\u5316<\/strong>Stress<\/strong>\u5206\u6790<\/strong>\u00a0<\/strong>Glutathione\u7a40\u80f1\u7518\u80bd\u7cfb\u5217\u5206\u6790<\/a><\/strong>\u00a0|\u00a0(SOD) \u6d3b\u6027\u5206\u6790\u5957\u7d44<\/a>\u00a0|\u00a0<\/strong>\u5f57\u661f\u5206\u6790\u5957\u7d44<\/a>\u00a0|\u7e3d\u6297\u6c27\u5316\u80fd\u529b\u5206\u6790<\/a>\u00a0|\u00a0TUNEL IHC\u67d3\u8272\u5957\u7d44<\/a><\/strong><\/span><\/p>\n

\u5f15\u7528\u6587\u737b<\/p>\n

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Marni E. Cueno, et al., (2018) Gingival Periodontal Disease (PD) Level-Butyric Acid Affects the Systemic Blood and Brain Organ: Insights Into the Systemic Inflammation of Periodontal Disease,\u00a0Frontiers in Microbiology, Jun. 2018, 29915575<\/a><\/div>\n<\/div>\n
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Yanru Zhao, et al., (2018) MiR-124 aggravates failing hearts by suppressing CD151-facilitated angiogenesis in heart,\u00a0Oncotarget, Jan. 2018, 29581851<\/a><\/div>\n<\/div>\n
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Rzymski, P. et al., (2017) Polyphasic toxicological screening of Cylindrospermopsis raciborskii and Aphanizomenon gracile isolated in Poland,\u00a0Algal Research, Jun.2017, 24: 72-80<\/a><\/div>\n<\/div>\n
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Baysal, M et al., (2017) Reproductive toxicity after levetiracetam administration in male rats: Evidence for role of hormonal status and oxidative stress,\u00a0PLoS One, 2017, 12(4): e0175990<\/a><\/div>\n<\/div>\n
\n
Ying et al., Targeted Deletion of p53 in the Proximal Tubule Prevents Ischemic Renal Injury.\u00a0J. Am. Soc. Nephrol.,\u00a0Dec 2014; 25: 2707 – 2716.<\/a><\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

\u8102\u8cea\u904e\u6c27\u5316\uff08MDA\uff09\u6bd4\u8272\/\u87a2\u5149\u6e2c\u5b9a\u8a66\u5291\u76d2\u00a0|\u00a0\u8ca8\u865fK739\u00a0(ABCAM ab118970) Lipid Pe […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[466,124,1],"tags":[3191],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14091"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=14091"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14091\/revisions"}],"predecessor-version":[{"id":40275,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14091\/revisions\/40275"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=14091"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=14091"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=14091"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}