{"id":14086,"date":"2017-09-21T12:40:29","date_gmt":"2017-09-21T04:40:29","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=14086"},"modified":"2022-07-21T10:35:20","modified_gmt":"2022-07-21T02:35:20","slug":"minute%e5%96%ae%e7%b4%b0%e8%83%9e%e5%88%86%e9%9b%a2%e8%a9%a6%e5%8a%91%e7%9b%92-for-fresh-and-fixed-tissues-%e8%b2%a8%e8%99%9fcat-sc-012","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=14086","title":{"rendered":"Minute\u2122\u55ae\u7d30\u80de\u5206\u96e2\u8a66\u5291\u76d2|Single Cell Isolation Kit\u8ca8\u865fCat# SC-012"},"content":{"rendered":"

Minute\u2122<\/strong>\u55ae\u7d30\u80de\u5206\u96e2\u8a66\u5291\u76d2<\/strong>|<\/strong>\u00a0(For fresh and fixed tissues)\u00a0<\/strong>\u8ca8\u865f<\/strong>Cat# SC-012<\/strong><\/a><\/span><\/p>\n

\u7522\u54c1\u4ecb\u7d39:<\/strong>\u00a0Invent Biotechnologies Minute\u2122\u55ae\u7d30\u80de\u5206\u96e2\u8a66\u5291\u76d2 (\u00a0Minute\u2122 Single Cell Isolation Kit) \uff0c\u5177\u6709\u512a\u5316\u7684\u7d44\u7e54\u89e3\u805a\u7de9\u885d\u6db2 (tissue disaggregation buffers)\uff0c\u548c\u7279\u6b8a\u8a2d\u8a082.0ml\u6536\u96c6\u7ba1\u7684\u6ffe\u7b52\u6280\u8853 (\u00a0filter cartridges) \u7d44\u6210\u3002\u8a72\u8a66\u5291\u76d2\u61c9\u7528\u65bc\u9ad8\u7d30\u80de\u6d3b\u529b\u7684\u65b0\u9bae\u52d5\u7269\u7d44\u7e54\u4e2d (\u00a0fresh animal tissues) \u5feb\u901f\u5206\u96e2\u55ae\u7d30\u80de (\u00a0single cells ) \u3002\u5b83\u4e5f\u53ef\u7528\u65bc\u5f9e\u7528\u7532\u919b(formaldehyde)\u77ed\u66ab\u56fa\u5b9a\u7684\u67d3\u8272\u9ad4\u514d\u75ab\u6c89\u6fb1\uff08ChIP\uff09\u4e2d\u5206\u96e2\u7d30\u80de\u3002\u5c07\u7d44\u7e54\u89e3\u805a\u7de9\u885d\u6db2 (tissue disaggregation buffers) \u914d\u88fd\u6210\u8f15\u8f15\u5730\u5206\u89e3\u52d5\u7269\u7d44\u7e54\u3002\u7de9\u885d\u6db2\u4e0d\u542b\u4efb\u4f55\u53ef\u80fd\u5c0d\u7d30\u80de\u8868\u9762\u6a19\u8a18\u7269 (cell surface markers) \u6709\u4e0d\u826f\u5f71\u97ff\u7684\u86cb\u767d\u9176 (\u00a0proteinases) \u548cEDTA\u3002\u7531\u65bc\u4f7f\u7528\u5177\u6709\u9810\u5b9a\u5b54\u5f91\u7684filter cartridges\u6ffe\u7b52\u6280\u8853\u548c\u5c08\u9580\u914d\u88fd\u7684\u7de9\u885d\u7cfb\u7d71\uff0c\u64cd\u4f5c\u53ef\u4ee5\u5728\u4e0d\u523010\u5206\u9418\u5167\uff0c\u5f9e\u65b0\u9bae\u7d44\u7e54\u7372\u5f97\u55ae\u7d30\u80de\u61f8\u6d6e\u6db2\u3002\u8a66\u5291\u76d2\u7684\u529f\u6548\u5177\u6709\u7d44\u7e54\u578b\u4f9d\u8cf4\u6027(\u00a0tissue type dependent)\uff1b\u8a72\u8a66\u5291\u76d2\u5c0d\u6dcb\u5df4\u7d44\u7e54\u5982\u813e\u81df (spleen)\u3001\u80f8\u817a(thymus) \u3001\u548c\u6dcb\u5df4\u7d50 (lymph node) \u7279\u5225\u6709\u6548\u3002\u5c0d\u65bc\u5177\u6709\u5927\u91cf\u7d50\u7de0\u7d44\u7e54\u542b\u91cf\u7684\u65b0\u9bae\u7d44\u7e54\uff0c\u6548\u679c\u662f\u53ef\u8b8a\u7684 (variable)\u3002\u4e0d\u9069\u7528\u65bc\u65b0\u9bae\u7684\u7d44\u7e54\u5982\u808c\u8089(muscle)\uff0c\u8178(intestine) \u548c\u80ba (lungs)\u3002\u5f9e\u51b7\u51cd\/\u65b0\u9bae\u7d44\u7e54\u4e2d\u7372\u53d6\uff0c\u8acb\u53c3\u95b1\u8ca8\u865fCS-031\u3002<\/p>\n

\"\"<\/p>\n

\"\"<\/p>\n

\u7522\u54c1\u61c9\u7528\uff1a\u63d0\u53d6\u7684\u55ae\u7d30\u80de\u53ef\u7528\u65bc\u67d3\u8272\u9ad4\u514d\u75ab\u5171\u6c89\u6fb1\uff08ChIP\uff09\u548cFACS\u5206\u6790\u3002\u55ae\u7d30\u80de\u61f8\u6d6e\u6db2\u4e5f\u53ef\u7528\u65bc\u5206\u96e2\/\u7d14\u5316DNA\uff0cRNA\uff0c\u86cb\u767d\u548c\u5176\u4ed6\u7d30\u80de\u7d44\u5206\u3002\u7279\u9ede\uff1a\u7c21\u55ae\u3001\u5feb\u901f\u3001\u6548\u7387\u9ad8\uff0c\u4e0d\u542b\u4efb\u4f55\u86cb\u767d\u9176\uff0c\u53ef\u5f9e\u65b0\u9bae\/\u51b7\u51cd\/\u7532\u919b\u56fa\u5b9a\u7684\u52d5\u7269\u7d44\u7e54\u4e2d\u5206\u96e2\u55ae\u7d30\u80de\u3002\u5132\u5b58\u689d\u4ef6\uff1a4\u2103<\/p>\n

Minute\u2122<\/strong>\u86cb\u767d\u8403\u53d6\u5957\u7d44\uff0c\u4f7f\u7528\u5275\u65b0\u300e<\/strong>Column Filter<\/strong>\u300f\u6280\u8853\uff0c\u6548\u679c\u66f4\u4f73\uff0c\u5b8c\u6210\u53ea\u9700<\/strong>\u00a0<\/strong>\u4e00<\/strong>\u00a0\u5206\u9418\u86cb\u767d\u8403\u53d6\u53ea\u9700\u8981<\/strong>vortex<\/em><\/strong>\u3001<\/em><\/strong>transfer<\/em><\/strong>\u3001<\/em><\/strong>spin\u00a0<\/em><\/strong>\u3002<\/em><\/strong>Minute \u2122\u4e00\u7cfb\u5217\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\uff1b\u9069\u7528\u65bc\u591a\u7a2e\u7269\u7a2e\uff08\u5305\u62ec\u54fa\u4e73\u52d5\u7269\u3001\u6d77\u6d0b\u52d5\u7269\u3001\u6606\u87f2\u3001\u690d\u7269\u548c\u7d30\u83cc\uff09\u5feb\u901f\u548c\u5bb9\u6613\u5730\u63d0\u53d6\/\u5206\u96e2\u86cb\u767d\u8cea\u7684\u512a\u7570\u5de5\u5177 \u3002\u8207\u5e02\u5834\u4e0a\u7684\u4efb\u4f55\u5176\u4ed6\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\u4e0d\u540c\uff0c\u512a\u5316\u7684\u7de9\u885d\u6db2\u7cfb\u7d71\uff0c\u7d50\u5408\u6700\u65b0\u7684\u5c08\u6709\u6ffe\u7b52\u6280\u8853\uff08spin column\uff09\uff1b\u5feb\u901f\u63d0\u9ad8\u86cb\u767d\u8403\u53d6\u7684\u6548\u80fd\u53ca\u65b9\u4fbf\u6027\uff1b\u7528\u65bc\u7e3d\u86cb\u767d\u63d0\u53d6\uff0c\u7d30\u80de\u5206\u96e2\u548c\u819c\u86cb\u767d\u5206\u96e2\uff0c\u53ea\u9700\u7c21\u55ae\u6b65\u9a5f\u5373\u53ef\u5b8c\u6210\u86cb\u767d\u8cea\u8403\u53d6\u3002\u7121\u9808\u9577\u6642\u9593\u7b49\u5f85\u3001\u514d\u9664\u91cd\u8907\u6027\u7684wash\u6b65\u9a5f\u3002<\/p>\n

Single Cell # Per 50 mg fresh mouse tissue and viability<\/p>\n\n\n\n\n\n
Tissues<\/em><\/strong><\/td>\nBrain<\/em><\/strong><\/td>\nKidney<\/em><\/strong><\/td>\nLiver<\/em><\/strong><\/td>\nLung<\/em><\/strong><\/td>\nSpleen<\/em><\/strong><\/td>\n<\/tr>\n
Cell # in Millions<\/td>\n4-5<\/td>\n5-6<\/td>\n5-6<\/td>\n6-7<\/td>\n15-16<\/td>\n<\/tr>\n
Viability (%)<\/td>\n40-50<\/td>\n50-55<\/td>\n70-75<\/td>\n70-80<\/td>\n90-95<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\"<\/p>\n

Single Cell# Per 50 mg formaldehyde fixed mouse tissue<\/p>\n\n\n\n\n
Tissues<\/em><\/strong><\/td>\nBrain<\/em><\/strong><\/td>\nKidney<\/em><\/strong><\/td>\nLiver<\/em><\/strong><\/td>\nLung<\/em><\/strong><\/td>\nSpleen<\/em><\/strong><\/td>\n<\/tr>\n
Cell # in Millions<\/td>\n4-5<\/td>\n4-5<\/td>\n4-5<\/td>\n5-6<\/td>\n13-15<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n
Item<\/td>\nAmount<\/td>\nPart #<\/td>\n<\/tr>\n
Buffer A (for non-fixed tissues)<\/td>\n25 ml<\/td>\nSC-012-N<\/td>\n<\/tr>\n
Buffer B (for fixed tissues)<\/td>\n25 ml<\/td>\nSC-012-F<\/td>\n<\/tr>\n
Cell isolation filter cartridges<\/td>\n50<\/td>\nP-001<\/td>\n<\/tr>\n
Collection Tubes with Cap<\/td>\n50<\/td>\nP-002<\/td>\n<\/tr>\n
plastic rods<\/td>\n4<\/td>\nP-003<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
    \n
  1. Kuo C-T, et al. (2016). Optical painting and fluorescence activated sorting of single adherent cells labelled with photoswitchable Pdots. Nature Communications. http:\/\/www.nature.com\/naturecommunications.<\/li>\n
  2. Bultmann-Mellin, I., Dinger, K., Debuschewitz, C., Loewe, K. M., Melcher, Y., Plum, M. T., … & J\u00fcngst, C. (2017). Role of LTBP-4 in alveolarization, angiogenesis and fibrosis in lungs. American Journal of Physiology-Lung Cellular and Molecular Physiology, ajplung-00031.<\/li>\n
  3. Vergara, M. N., Flores-Bellver, M., Aparicio-Domingo, S., McNally, M., Wahlin, K. J., Saxena, M. T., … & Canto-Soler, M. V. (2017). Enabling quantitative screening in retinal organoids: 3D automated reporter quantification technology (3D-ARQ). Development, dev-146290.<\/li>\n
  4. Janicova, A., Becker, N., Xu, B., Wutzler, S., Vollrath, T., Hildebrand, F., … & Relja, B. (2019). Endogenous uteroglobin as intrinsic anti-inflammatory signal modulates monocyte and macrophage subsets distribution upon sepsis induced lung injury.\u00a0Frontiers in Immunology<\/em>,\u00a010<\/em>, 2276.<\/li>\n
  5. Tanaka, M., Saka-Tanaka, M., Ochi, K., Fujieda, K., Sugiura, Y., Miyamoto, T., … & Aoe, S. (2020). C-type lectin Mincle mediates cell death\u2013triggered inflammation in acute kidney injury.\u00a0Journal of Experimental Medicine<\/em>,\u00a0217<\/em>(11).<\/li>\n<\/ol>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
    Cat. No<\/td>\nStarting Materials<\/td>\nStatus of Extracted Proteins<\/td>\nYield
    \n(mg\/ml)<\/td>\n    		Protocol\u00a0Time
    \n(min)<\/td>\n<\/tr>\n
    SD-001\/ SN-002<\/td>\nCultured Animal Cells and Tissues<\/td>\nDenatured\/Native<\/td>\n2-8<\/td>\n1-8<\/td>\n<\/tr>\n
    SC-003<\/td>\nMammalian Cultured Cells\/Tissues<\/td>\nNative<\/td>\n1-3<\/td>\n6-8<\/td>\n<\/tr>\n
    SN-006<\/td>\nCultured Animal Cells and Tissues<\/td>\nDenatured\/Native<\/td>\n1-4<\/td>\n5-8<\/td>\n<\/tr>\n
    SM-005<\/td>\nMammalian Cultured Cells\/Tissues<\/td>\nNative Detergent\/ EDTA -Free<\/td>\n1-3<\/td>\n20-45<\/td>\n<\/tr>\n
    MP-007<\/td>\nMammalian Cultured Cells\/Tissues<\/td>\nNative Detergent\/ EDTA-Free<\/td>\n1-3<\/td>\n25-30<\/td>\n<\/tr>\n
    SD-008\/ SN-009<\/td>\nFresh Plant Tissues<\/td>\nDenatured\/Native<\/td>\n2-8<\/td>\n5-8<\/td>\n<\/tr>\n
    SN-010<\/td>\nFresh Plant Tissues<\/td>\nNative<\/td>\n1-5<\/td>\n6-8<\/td>\n<\/tr>\n
    CP-011<\/td>\nFresh Plant Tissues (5-200 mg\/sample)<\/td>\nChloroplast (>90%) Intact<\/td>\n1-4<\/td>\n5<\/td>\n<\/tr>\n
    SB-004<\/td>\nCultured Bacterial Cells<\/td>\nDenatured<\/td>\n2-3<\/td>\n2-3<\/td>\n<\/tr>\n
    NE-013<\/td>\nCultured cells or Cells from Tissues<\/td>\nNative<\/td>\n1-3<\/td>\n45<\/td>\n<\/tr>\n
    HP-014<\/td>\nCultured cells or Cells from Tissues<\/td>\nDenatured<\/td>\n1-2.5<\/td>\n<10<\/td>\n<\/tr>\n
    YT-015<\/td>\nCultured Microbes<\/td>\nDenatured\/Native<\/td>\n2-5<\/td>\n<10 min<\/td>\n<\/tr>\n
    YD-016<\/td>\nCultured Microbes<\/td>\nDenatured\/Native<\/td>\n2-4<\/td>\n<10 min<\/td>\n<\/tr>\n
    YM-017<\/td>\nCultured Yeast<\/td>\nNative<\/td>\n2-3<\/td>\n1h<\/td>\n<\/tr>\n
    MM-018<\/td>\nPlant tissues<\/td>\nNative<\/td>\n2-3<\/td>\n1h<\/td>\n<\/tr>\n
    PN-019<\/td>\nPAGE\/Agarose gels<\/td>\nDenatured\/Native<\/td>\nNA<\/td>\n10-20 min<\/td>\n<\/tr>\n
    HD-021<\/td>\nKeratinized tissues<\/td>\nDenatured<\/td>\n1-2<\/td>\n5 min hand on<\/td>\n<\/tr>\n
    AT-022<\/td>\nAdipose Tissues\/Adipocytes<\/td>\nNative\/Denatured<\/td>\n2-3<\/td>\n20 min<\/td>\n<\/tr>\n
    AF-023<\/td>\nAdipose Tissues\/Adipocytes<\/td>\nNative<\/td>\n0.5-1<\/td>\n20 min<\/td>\n<\/tr>\n
    FE-025<\/td>\nFFPE<\/td>\nDenatured<\/td>\n2-3<\/td>\n1h<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

     <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
    Sub-Cellular Structure Fractionation\/Isolation<\/strong><\/td>\n<\/tr>\n
    Cat #<\/strong><\/td>\nDescription<\/strong><\/td>\nSize<\/strong><\/td>\n<\/td>\n<\/tr>\n
    SC-003<\/a><\/td>\nCytoplasmic & Nuclear Extraction Kits<\/a><\/td>\n50 Preps<\/td>\n<\/td>\n<\/tr>\n
    SM-005<\/a><\/td>\nPlasma Membrane Protein Isolation Kit<\/a><\/td>\n50 preps<\/td>\n<\/td>\n<\/tr>\n
    NE-013<\/a><\/td>\nNuclear Envelope Protein Extraction Kit<\/a><\/td>\n50 preps<\/td>\n<\/td>\n<\/tr>\n
    MP-007<\/a><\/td>\nMitochondria Isolation Kit For Mammalian Cells And Tissues<\/a><\/td>\n50 preps<\/td>\n<\/td>\n<\/tr>\n
    AF-023<\/a><\/td>\nAdipose Tissue Fractionation Kit<\/a><\/td>\n20 preps<\/td>\n<\/td>\n<\/tr>\n
    NI-024<\/a><\/td>\nDetergent-free nuclei isolation kit<\/a><\/td>\n20 preps<\/td>\n<\/td>\n<\/tr>\n
    YM-017<\/a><\/td>\nYeast Mitochondria Enrichment Kit<\/a><\/td>\n50 preps<\/td>\n<\/td>\n<\/tr>\n
    MM-018<\/a><\/td>\nPlant Microsomal Membrane Extraction Kit<\/a><\/td>\n50 preps<\/td>\n<\/td>\n<\/tr>\n
    CP-011<\/a><\/td>\nChloroplast Isolation Kit<\/a><\/td>\n50 preps<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

     <\/p>\n

     <\/p>\n

     <\/p>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    Minute\u2122\u55ae\u7d30\u80de\u5206\u96e2\u8a66\u5291\u76d2|\u00a0(For fresh and fixed tissues)\u00a0\u8ca8\u865fCat# S […]<\/p>\n","protected":false},"author":3,"featured_media":14087,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[179],"tags":[3192],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14086"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=14086"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14086\/revisions"}],"predecessor-version":[{"id":26571,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14086\/revisions\/26571"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/14087"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=14086"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=14086"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=14086"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}