{"id":14058,"date":"2017-09-18T17:48:01","date_gmt":"2017-09-18T09:48:01","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=14058"},"modified":"2022-07-21T10:35:27","modified_gmt":"2022-07-21T02:35:27","slug":"%e6%b0%b8%e7%94%9f%e5%8c%96%e5%b0%8f%e9%bc%a0%e8%83%b0%e8%85%ba%e4%b8%8a%e7%9a%ae%e7%b4%b0%e8%83%9e-immortalized-conditionally-mouse-pancreas-epithelial-cells-impe-%e8%b2%a8%e8%99%9ft0156","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=14058","title":{"rendered":"\u6c38\u751f\u5316\u5c0f\u9f20\u80f0\u817a\u4e0a\u76ae\u7d30\u80de | Immortalized (Conditionally) Mouse Pancreas Epithelial Cells (IMPE) \u8ca8\u865fT0156"},"content":{"rendered":"

\"ABM<\/a>\u00a0\u6c38\u751f\u5316\uff08Conditional\uff09\u5c0f\u9f20\u80f0\u817a\u4e0a\u76ae\u7d30\u80de\uff08IMPE\uff09 \u00a0| <\/strong>\u8ca8\u865f<\/strong>T0156<\/strong><\/a><\/span><\/p>\n

\u00a0<\/strong>Immortalized (Conditionally) Mouse Pancreas Epithelial Cells (IMPE)<\/p>\n

\"immortalized<\/p>\n

IMPE\u662f\u5728\u5e72\u64fe\u7d20\uff08IFN\uff09-\u03b3\u53ef\u8a98\u5c0e\u7684H-2Kb\u555f\u52d5\u5b50\u548c\u6eab\u5ea6\u654f\u611f\u7684\u733f\u7334\u75c5\u6bd240\u5927T\u6297\u539f\u7684\u63a7\u5236\u4e0b\u5f9e\u651c\u5e36\u4e0d\u8010\u71b1\u7a81\u8b8a\uff08tsA58\uff09\u7684\u5c0f\u9f20\u5206\u96e2\u7684\u689d\u4ef6\u6c38\u751f\u5c0f\u9f20\u80f0\u817a\u4e0a\u76ae\u7d30\u80de\u7cfb\u3002 SV40\u5927T\u6297\u539f\u7684\u529f\u80fd\u8868\u9054\u662f\u901a\u904e\u5728\u6eab\u5ea6\u5141\u8a31\uff0833\u2103\uff09\u4e0b\u5728\u542b\u6709IFN\u03b3\u7684\u57f9\u990a\u57fa\u4e2d\u9ad4\u5916\u57f9\u990a\u7d30\u80de\u4f86\u8a98\u5c0e\u7684\u3002 \u5728\u4e0d\u5141\u8a31\u7684\u6eab\u5ea6\uff0837\u2103-39\u2103\uff09\u4e0b\uff0c\u6216\u8005\u7576\u7d933\u4ee3\u50b3\u4ee3\u57f9\u990a\u7121IFN\u03b3\u6642\uff0c\u7d30\u80de\u4e0d\u518d\u589e\u6b96\u3002 \u5df2\u986f\u793aIMPE\u5728\u7528pdx1\u8f49\u67d3\u5f8c\u7522\u751f\u80f0\u5cf6\u7d20\uff0c\u4e26\u5728\u80f0\u9ad8\u8840\u7cd6\u7d20\u6a23\u80bd1\uff08GLP1\uff09\u5b58\u5728\u4e0b\u57f9\u990a\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n
BioSafety Level<\/strong><\/td>\nII<\/td>\n<\/tr>\n
Organism<\/strong><\/td>\nH-2Kb<\/sup>-tsA58 transgenic mouse<\/td>\n<\/tr>\n
Source Organ<\/strong><\/td>\nPancreas<\/td>\n<\/tr>\n
Growth Properties<\/strong><\/td>\nAdherent<\/td>\n<\/tr>\n
Morphology<\/strong><\/td>\nCobblestone-like<\/td>\n<\/tr>\n
Recommended Seeding Density<\/strong><\/td>\nRecommended split ratio of 1:3<\/td>\n<\/tr>\n
Markers<\/strong><\/td>\n\u03b2-catenin, E-Cadherin, CK8, CK18, CK19<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nFor Research Use Only<\/td>\n<\/tr>\n
Immortalization Method<\/strong><\/td>\nIsolated from transgenic mouse carrying a temperature-sensitive simian virus 40 tumor antigen (tsA58)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

A<\/strong>bmgood<\/strong>\u4e0d<\/strong>\u673d<\/strong>\u5316\u7cfb\u5217\u5546\u54c1<\/strong> (immortalized cell)<\/strong><\/p>\n

\u6839\u64da\u7d30\u80de\u985e\u578b\uff0cPrimary cells\u521d\u4ee3\u7d30\u80de\u5728\u9ad4\u5916\u5177\u6709\u5c0f\u65bc10-50\u6b21\u50b3\u4ee3\u7684\u6709\u9650\u58fd\u547d\u3002 \u4e0d\u673d\u5316\u7d30\u80de\u682a\u80fd\u5920\u5ef6\u9577\u589e\u6b96\uff0c\u4e26\u4e14\u5177\u6709\u8207\u89aa\u4ee3\u7d44\u7e54\u76f8\u4f3c\u6216\u76f8\u540c\u7684\u57fa\u56e0\u578b\u548c\u8868\u73fe\u578b\u3002Abmgood\u591a\u5e74\u7814\u767c\u7d93\u9a57\uff0c\u958b\u767c\u51fa\u4e86\u4e00\u7cfb\u5217Immortalized\u5546\u54c1\u3002\u54fa\u4e73\u52d5\u7269\u7d30\u80de\u7684<\/strong>\u4e0d<\/strong>\u673d<\/strong>\u5316Immortalized\u7b56\u7565<\/strong>\uff1b\u5b58\u5728\u7528\u65bc\u5728\u57f9\u990a\u7269\u4e2d\u4e0d<\/strong>\u673d<\/strong>\u5316<\/strong>\u54fa\u4e73\u52d5\u7269\u7d30\u80de\u7684\u5e7e\u7a2e\u65b9\u6cd5\u3002\uff1b\u5305\u62ec\u5373\u7528\u578b\u9006\u8f49\u9304\u75c5\u6bd2\uff0c\u6162\u75c5\u6bd2\u548c\u817a\u75c5\u6bd2\u8f09\u9ad4\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Gene Overexpressed<\/strong><\/td>\nViruses Offered<\/strong><\/td>\nApplication<\/strong><\/td>\n<\/tr>\n
hTERT<\/a><\/td>\nAdenovirus,
\nLentivirus,
\nRetrovirus<\/td>\n		\u53ef\u9760\u548c\u5e38\u7528\u3002 \u53ef\u80fd\u5728\u4e00\u4e9b\u7d30\u80de\u985e\u578b\uff08\u4f8b\u5982\u4e0a\u76ae\u7d30\u80de\uff09\u4e2d\u5f15\u8d77\u7d30\u80de\u6b7b\u4ea1<\/td>\n<\/tr>\n
SV40 T Antigen<\/a><\/td>\nAdenovirus,
\nLentivirus,
\nRetrovirus<\/td>\n		\u53ef\u9760\u548c\u5e38\u7528\u3002 \u901a\u5e38\u7528\u65bc\u4e0d\u673d\u5316\u4e0a\u76ae\u7d30\u80de\u3002<\/td>\n<\/tr>\n
EBV genes<\/a><\/td>\nEpstein-Barr Virus<\/td>\n\u4e0d\u673d\u5316B\u6dcb\u5df4\u7d30\u80de\uff0c\u6709\u6642\u662fT\u6dcb\u5df4\u7d30\u80de\u3002<\/td>\n<\/tr>\n
HPV E6-E7<\/a><\/td>\nLentivirus<\/td>\n\u4e0d\u673d\u5316\u89d2\u5316\u7d30\u80de\u3002<\/td>\n<\/tr>\n
HOXB8, HOXA9, HOXA10<\/a><\/td>\nLentivirus<\/td>\n\u4e0d\u673d\u5316\u9020\u8840\u7d30\u80de\uff0c\u5305\u62ec\u5de8\u566c\u7d30\u80de\uff0c\u9020\u8840\u7956\u7d30\u80de\u548c\u9aa8\u9ad3\u7956\u7d30\u80de\u3002<\/td>\n<\/tr>\n
Adenoviral E1A-E1B<\/a><\/td>\nLentivirus<\/td>\n\u4e0d\u673d\u5316\u5927\u7bc4\u570d\u7684\u521d\u7d1a\u56d3\u9f52\u52d5\u7269\u7d30\u80de\uff0c\u5305\u62ec\u7f8a\u6c34\u7d30\u80de\uff0c\u539f\u767c\u6027\u4e0a\u76ae\u7d30\u80de\uff0c\u6210\u7e96\u7dad\u7d30\u80de\u548c\u539f\u4ee3\u814e\u7d30\u80de\u3002<\/td>\n<\/tr>\n
p53, Rb siRNA<\/a><\/td>\nLentivirus<\/td>\n\u8207hTERT\u5171\u8868\u9054\u4ee5\u66f4\u597d\u5730\u4f7f\u4e0a\u76ae\u7d30\u80de\u4e0d\u673d\u5316\u3002<\/td>\n<\/tr>\n
Bmi1<\/a><\/td>\nLentivirus<\/td>\n\u4e0d\u673d\u5316\u4e0a\u76ae\u7d30\u80de\u3002<\/td>\n<\/tr>\n
CDK4<\/a><\/td>\nLentivirus<\/td>\n\u8207hTERT\u5171\u540c\u8868\u9054\u66f4\u771f\u5be6\u7684\u7d30\u80de\u6a21\u578b\u3002<\/td>\n<\/tr>\n
Myc T58A (c-Myc), Ras V12 (H-Ras)<\/a><\/td>\nLentivirus<\/td>\n\u4e0d\u673d\u5316\u5404\u7a2e\u7d30\u80de\uff0c\u5305\u62ec\u795e\u7d93\u5e79\u7d30\u80de\uff0c\u521d\u4ee3\u814e\u7d30\u80de\uff0c\u6210\u7d05\u7d30\u80de\u548c\u5404\u7a2e\u4e0a\u76ae\u7d30\u80de\uff08\u539f\u767c\u6027\u524d\u5217\u817a\uff0c\u4e73\u817a\uff0c\u6c23\u9053\u7b49\uff09\u3002<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u76f8\u95dc\u8a66\u5291\"<\/strong>\u00a0<\/u><\/span>\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c<\/span><\/a>\u00a0|<\/strong>\u00a0DAPI\u67d3\u6838<\/a>\u00a0|<\/strong>\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291<\/a>\u00a0|<\/strong>\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291<\/a>\u00a0|<\/strong>\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49\u7cfb\u5217\u5546\u54c1<\/a><\/span><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u00a0\u6c38\u751f\u5316\uff08Conditional\uff09\u5c0f\u9f20\u80f0\u817a\u4e0a\u76ae\u7d30\u80de\uff08IMPE\uff09 \u00a0| \u8ca8\u865fT0156 \u00a0Immortalize […]<\/p>\n","protected":false},"author":3,"featured_media":11653,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[453],"tags":[3193],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14058"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=14058"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14058\/revisions"}],"predecessor-version":[{"id":14065,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14058\/revisions\/14065"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/11653"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=14058"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=14058"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=14058"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}