{"id":14039,"date":"2017-09-18T14:06:37","date_gmt":"2017-09-18T06:06:37","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=14039"},"modified":"2022-07-21T10:35:45","modified_gmt":"2022-07-21T02:35:45","slug":"tlr9%e5%9f%ba%e5%9b%a0%e5%89%94%e9%99%a4-tlr9-crispr-knock-out-a549-cell-line-human-%e8%b2%a8%e8%99%9fk0163157","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=14039","title":{"rendered":"TLR9\u57fa\u56e0\u5254\u9664 | TLR9 CRISPR Knock Out A549 Cell Line (Human) \u8ca8\u865fK0163157"},"content":{"rendered":"

\"ABM<\/a>\u00a0TLR9 CRISPR Knock Out A549 Cell Line (Human)<\/strong> |<\/strong>\u8ca8\u865f<\/strong>K0163157<\/strong><\/span><\/p>\n

CRISPR\u7d30\u80de\u8cc7\u6599: TLR9 Accession Number NM_017442<\/a> | A549\u00a0pLenti-U6-sgRNA-PGK-Neo<\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Accession Number<\/strong><\/td>\nNM_017442<\/a><\/td>\n<\/tr>\n
Vector<\/strong><\/td>\npLenti-U6-sgRNA-PGK-Neo<\/a><\/td>\n<\/tr>\n
Target Sequence<\/strong><\/td>\nSequence available upon order placement<\/td>\n<\/tr>\n
Format<\/strong><\/td>\nStable cell pool with sequence verified knockout<\/td>\n<\/tr>\n
Parental Cell Line<\/strong><\/td>\nA549<\/td>\n<\/tr>\n
Drug resistance<\/strong><\/td>\nPuromycin (0.5\u03bcg\/ml)<\/td>\n<\/tr>\n
Culture Condition<\/strong><\/td>\nCulture Condition The base medium for this cell line is TM003, 10%FBS and 1%P\/S, 37\u00b0C, 5% CO2<\/sub><\/td>\n<\/tr>\n
Storage<\/strong><\/td>\n-180\u00b0C<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nShipped with dry ice<\/td>\n<\/tr>\n
Additional Materials<\/strong><\/td>\n–\u00a0CRISPR Genomic Cleavage Detection Kit (G932)<\/a><\/td>\n<\/tr>\n
Quality Control<\/strong><\/td>\nMismatch Cleavage assay and Sanger sequencing to confirm indels in addition to microbial contaminant testing<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u76f8\u95dc\u5546\u54c1 CRISPR\u6aa2\u6e2c\u5957\u7d44<\/a> | CRISPR\u57fa\u56e0\u5254\u9664\u7d30\u80de\u682a<\/a> | CASPASE9 (D10A) NLS<\/a><\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u00a0TLR9 CRISPR Knock Out A549 Cell Line (Human) |\u8ca8\u865fK01631 […]<\/p>\n","protected":false},"author":3,"featured_media":10635,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[453],"tags":[3196],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14039"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=14039"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14039\/revisions"}],"predecessor-version":[{"id":14070,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14039\/revisions\/14070"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/10635"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=14039"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=14039"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=14039"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}