{"id":14032,"date":"2017-09-18T13:31:26","date_gmt":"2017-09-18T05:31:26","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=14032"},"modified":"2022-07-21T10:35:51","modified_gmt":"2022-07-21T02:35:51","slug":"tlr9%e6%95%b2%e9%99%a4%e4%b9%8b%e6%b0%b8%e7%94%9f%e5%8c%96%e5%b0%8f%e9%bc%a0%e6%a8%b9%e7%aa%81%e7%8b%80%e7%b4%b0%e8%83%9e-tlr9-knockout-immortalized-mouse-dendritic-cell-line%e8%b2%a8%e8%99%9ft3035","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=14032","title":{"rendered":"TLR9\u6572\u9664\u4e4b\u6c38\u751f\u5316\u5c0f\u9f20\u6a39\u7a81\u72c0\u7d30\u80de | TLR9 Knockout Immortalized Mouse Dendritic Cell Line\u8ca8\u865fT3035"},"content":{"rendered":"

\"ABM\u00a0TLR9<\/strong>\u6572\u9664<\/strong>\u4e4b<\/strong>\u6c38\u751f\u5316\u5c0f\u9f20\u6a39\u7a81\u72c0\u7d30\u80de | <\/strong>\u8ca8\u865f<\/strong>T3035<\/strong><\/a><\/span><\/p>\n

TLR9 Knockout Immortalized Mouse Dendritic Cell Line<\/p>\n

\u4f5c\u70ba\u5148\u5929\u6027\u548c\u9069\u61c9\u6027\u514d\u75ab\u7cfb\u7d71\u7684\u4fe1\u4f7f\u6a4b\u63a5\uff0c\u50b3\u7d71\u7684\u7d44\u7e54\u99d0\u7559DC\uff08cDC\uff09\u4f5c\u70ba\u4e8c\u7d1a\u6dcb\u5df4\u5668\u5b98\u548c\u5176\u4ed6\u7d44\u7e54\u4e2d\u7684sentinels\uff0c\u7528\u65bc\u6297\u539f\u6355\u7372\u548c\u8868\u9054\u3002 MutuDCTlr9 – \/ – \u7d30\u80de\u4fc2\u662f\u6e90\u81eaTLR9\u6572\u9664CD11c\uff1aSV40LgT\u8f49\u57fa\u56e0\u5c0f\u9f20\u7684\u5e38\u898f\u6a39\u7a81\u72c0\u7d30\u80de\u7684\u6c38\u751f\u5316\u813eCD8\u03b1+\u5b50\u96c6\uff08CD11chigh\uff0cB220-\uff0cDEC205 +\uff0cCD24high\uff0cCD11b-\uff09\u3002MutuDCTlr9 – \/ – \u7d30\u80de\u4fdd\u7559\u5c0dTLR\u914d\u9ad4\u7684\u97ff\u61c9\uff0c\u5982PolyIC\uff08TLR3-L\uff09\u548c\u8f03\u5c0f\u7a0b\u5ea6\u7684LPS\uff08TLR4-L\uff09\u800c\u4e0d\u662fCpG\uff08TLR9-L\uff09\u3002\u50cf\u91ce\u751f\u578bMutuDC\u7d30\u80de\uff08\u8ca8\u865fT0528\uff09\u4e00\u6a23\uff0c\u9019\u4e9b\u7d30\u80de\u80fd\u5920\u5728MHC-1\u548cMHC-II\u5169\u8005\u7684\u4e0a\u4e0b\u6587\u4e2d\u5448\u905e\u6297\u539f\uff0c\u5305\u62ec\u7d30\u80de\u76f8\u95dc\u6297\u539f\u7684\u76f4\u63a5\u6297\u539f\u5448\u905e\u548c\u4ea4\u53c9\u8868\u9054\u3002\u8207TLR3\u6572\u9664\uff08\u8ca8\u865fT3034\uff09\u548cIfnar1\u6572\u9664\uff08Cat.No.T3036\uff09MutuDC\u4e00\u8d77\uff0c\u9019\u4e9b\u6a39\u7a81\u72c0\u7d30\u80de\u7cfb\u63d0\u4f9b\u4e86\u75ab\u82d7\u79d1\u5b78\u548c\u514d\u75ab\u6cbb\u7642\u4e2d\u7684\u5f37\u5927\u5de5\u5177\uff0c\u7279\u5225\u662f\u5728\u5c07\u9776\u6297\u539f\u7b56\u7565\u5316\u70baCD8\u03b1+\u4e9e\u578b\u7684\u904e\u7a0b\u4e2d\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
BioSafety Level<\/strong><\/td>\nII<\/td>\n<\/tr>\n
Organism<\/strong><\/td>\nCD11c:LgT-Tg x Tlr9(tm1.Aki) C57BL6 Mouse<\/td>\n<\/tr>\n
SourceOrgan<\/strong><\/td>\nSpleen<\/td>\n<\/tr>\n
Growth Properties<\/strong><\/td>\nAdherent<\/td>\n<\/tr>\n
Morphology<\/strong><\/td>\nSmall Aggregates<\/td>\n<\/tr>\n
Procedure Overview<\/strong><\/td>\n<\/td>\n<\/tr>\n
Recommended Seeding Density<\/strong><\/td>\n2.5 x 105\u00a0cells\/cm2, should not be split lower than 5 x 104\u00a0cells per cm2<\/td>\n<\/tr>\n
Markers<\/strong><\/td>\nCD11c, CD24, MHC-II+, DEC205, Clec9A, B220, CD11b, IRF4, IRF8, CD4<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nFor Research Use Only<\/td>\n<\/tr>\n
BioSafety Level<\/strong><\/td>\nII<\/td>\n<\/tr>\n
Organism<\/strong><\/td>\nHomo sapiens<\/td>\n<\/tr>\n
Source Organ<\/strong><\/td>\nUrinary Tissue<\/td>\n<\/tr>\n
Donor Gender<\/strong><\/td>\nMale<\/td>\n<\/tr>\n
Growth Properties<\/strong><\/td>\nAdherent<\/td>\n<\/tr>\n
Morphology<\/strong><\/td>\nCuboidal<\/td>\n<\/tr>\n
Recommended Seeding Density<\/strong><\/td>\nThaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nFor Research Use Only<\/td>\n<\/tr>\n
Immortalization Method<\/strong><\/td>\nCo-infected with retrovirus carrying the thermosensitive SV40 large T antigen (U19tsA58) and with retrovirus carrying human telomerase (hTERT)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\"<\/p>\n

\"\"<\/p>\n

\u7d30\u80de\u57f9\u990a: \u5efa\u8b70\u5728Poly-L-Lysine\u5305\u8863\u7684\u71d2\u74f6\u4e2d\u751f\u9577\u9019\u4e9b\u7d30\u80de\uff0c\u5176\u4e2d\u805a-L-\u8cf4\u6c28\u9178\u5305\u88ab\u6eb6\u6db2\uff080.1\uff05\uff09\u53ef\u7528\u65bcabm\uff08TM061\uff09\u3002 \u8a72\u7d30\u80de\u7cfb\u7684\u57fa\u790e\u57f9\u990a\u57fa\u662fPriGrow V\uff0c\u53ef\u5728abm\uff08TM015\uff09\u7372\u5f97\u3002 \u70ba\u4e86\u88fd\u5099\u5b8c\u6210\u7684\u751f\u9577\u57f9\u990a\u57fa\uff0c\u5c07\u4ee5\u4e0b\u7d44\u5206\u6dfb\u52a0\u5230\u57fa\u790e\u57f9\u990a\u57fa\u4e2d\uff1a10\uff05\u88dc\u5145\u7684\u80ce\u725b\u8840\u6e05\uff08PAN\u751f\u7269\u6280\u8853; P40-37500\uff09\uff0c50\u03bcM\u03b2-\u5df0\u57fa\u4e59\u9187\uff0c1\uff05HEPES\u548c1\uff05\u9752\u9ef4\u7d20\/\u93c8\u9ef4\u7d20\uff08G255\uff09\u3002 \u53ef\u4ee5\u4f7f\u7528PolyIC\uff085\u03bcg\/ ml\uff09\uff0cCpG\uff082mM\uff09\u6216LPS\uff085\u03bcg\/ ml\uff09\u9032\u884c\u523a\u6fc0\u3002 \u9019\u4e9b\u7d30\u80de\u5c0dFBS\u8981\u6c42\u7279\u5225\u654f\u611f\uff0c\u56e0\u6b64\uff0c\u5efa\u8b70\u4f7f\u7528\u76f8\u540c\u7684\u6279\u6b21\u4f86\u57f9\u990a\u986f\u793a\u6700\u4f73\u7d50\u679c\u652f\u6301\u5176\u57f9\u990a\u7684\u7d30\u80de\u3002 \u6211\u5011\u9084\u5efa\u8b70\u5728\u5b8c\u6574\u7684\u57f9\u990a\u57fa\u4e2d\u6dfb\u52a0\u984d\u5916\u76841\uff05Hepes\u4ee5\u4fc3\u9032\u7d30\u80de\u7684\u7e41\u6b96\u3002<\/p>\n

\"\"<\/p>\n

A<\/strong>bmgood<\/strong>\u4e0d<\/strong>\u673d<\/strong>\u5316\u7cfb\u5217\u5546\u54c1<\/strong> (immortalized cell)<\/strong><\/p>\n

\u6839\u64da\u7d30\u80de\u985e\u578b\uff0cPrimary cells\u521d\u4ee3\u7d30\u80de\u5728\u9ad4\u5916\u5177\u6709\u5c0f\u65bc10-50\u6b21\u50b3\u4ee3\u7684\u6709\u9650\u58fd\u547d\u3002 \u4e0d\u673d\u5316\u7d30\u80de\u682a\u80fd\u5920\u5ef6\u9577\u589e\u6b96\uff0c\u4e26\u4e14\u5177\u6709\u8207\u89aa\u4ee3\u7d44\u7e54\u76f8\u4f3c\u6216\u76f8\u540c\u7684\u57fa\u56e0\u578b\u548c\u8868\u73fe\u578b\u3002Abmgood\u591a\u5e74\u7814\u767c\u7d93\u9a57\uff0c\u958b\u767c\u51fa\u4e86\u4e00\u7cfb\u5217Immortalized\u5546\u54c1\u3002\u54fa\u4e73\u52d5\u7269\u7d30\u80de\u7684<\/strong>\u4e0d<\/strong>\u673d<\/strong>\u5316Immortalized\u7b56\u7565<\/strong>\uff1b\u5b58\u5728\u7528\u65bc\u5728\u57f9\u990a\u7269\u4e2d\u4e0d<\/strong>\u673d<\/strong>\u5316<\/strong>\u54fa\u4e73\u52d5\u7269\u7d30\u80de\u7684\u5e7e\u7a2e\u65b9\u6cd5\u3002\uff1b\u5305\u62ec\u5373\u7528\u578b\u9006\u8f49\u9304\u75c5\u6bd2\uff0c\u6162\u75c5\u6bd2\u548c\u817a\u75c5\u6bd2\u8f09\u9ad4\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Gene Overexpressed<\/strong><\/td>\nViruses Offered<\/strong><\/td>\nApplication<\/strong><\/td>\n<\/tr>\n
hTERT<\/a><\/td>\nAdenovirus,
\nLentivirus,
\nRetrovirus<\/td>\n		\u53ef\u9760\u548c\u5e38\u7528\u3002 \u53ef\u80fd\u5728\u4e00\u4e9b\u7d30\u80de\u985e\u578b\uff08\u4f8b\u5982\u4e0a\u76ae\u7d30\u80de\uff09\u4e2d\u5f15\u8d77\u7d30\u80de\u6b7b\u4ea1<\/td>\n<\/tr>\n
SV40 T Antigen<\/a><\/td>\nAdenovirus,
\nLentivirus,
\nRetrovirus<\/td>\n		\u53ef\u9760\u548c\u5e38\u7528\u3002 \u901a\u5e38\u7528\u65bc\u4e0d\u673d\u5316\u4e0a\u76ae\u7d30\u80de\u3002<\/td>\n<\/tr>\n
EBV genes<\/a><\/td>\nEpstein-Barr Virus<\/td>\n\u4e0d\u673d\u5316B\u6dcb\u5df4\u7d30\u80de\uff0c\u6709\u6642\u662fT\u6dcb\u5df4\u7d30\u80de\u3002<\/td>\n<\/tr>\n
HPV E6-E7<\/a><\/td>\nLentivirus<\/td>\n\u4e0d\u673d\u5316\u89d2\u5316\u7d30\u80de\u3002<\/td>\n<\/tr>\n
HOXB8, HOXA9, HOXA10<\/a><\/td>\nLentivirus<\/td>\n\u4e0d\u673d\u5316\u9020\u8840\u7d30\u80de\uff0c\u5305\u62ec\u5de8\u566c\u7d30\u80de\uff0c\u9020\u8840\u7956\u7d30\u80de\u548c\u9aa8\u9ad3\u7956\u7d30\u80de\u3002<\/td>\n<\/tr>\n
Adenoviral E1A-E1B<\/a><\/td>\nLentivirus<\/td>\n\u4e0d\u673d\u5316\u5927\u7bc4\u570d\u7684\u521d\u7d1a\u56d3\u9f52\u52d5\u7269\u7d30\u80de\uff0c\u5305\u62ec\u7f8a\u6c34\u7d30\u80de\uff0c\u539f\u767c\u6027\u4e0a\u76ae\u7d30\u80de\uff0c\u6210\u7e96\u7dad\u7d30\u80de\u548c\u539f\u4ee3\u814e\u7d30\u80de\u3002<\/td>\n<\/tr>\n
p53, Rb siRNA<\/a><\/td>\nLentivirus<\/td>\n\u8207hTERT\u5171\u8868\u9054\u4ee5\u66f4\u597d\u5730\u4f7f\u4e0a\u76ae\u7d30\u80de\u4e0d\u673d\u5316\u3002<\/td>\n<\/tr>\n
Bmi1<\/a><\/td>\nLentivirus<\/td>\n\u4e0d\u673d\u5316\u4e0a\u76ae\u7d30\u80de\u3002<\/td>\n<\/tr>\n
CDK4<\/a><\/td>\nLentivirus<\/td>\n\u8207hTERT\u5171\u540c\u8868\u9054\u66f4\u771f\u5be6\u7684\u7d30\u80de\u6a21\u578b\u3002<\/td>\n<\/tr>\n
Myc T58A (c-Myc), Ras V12 (H-Ras)<\/a><\/td>\nLentivirus<\/td>\n\u4e0d\u673d\u5316\u5404\u7a2e\u7d30\u80de\uff0c\u5305\u62ec\u795e\u7d93\u5e79\u7d30\u80de\uff0c\u521d\u4ee3\u814e\u7d30\u80de\uff0c\u6210\u7d05\u7d30\u80de\u548c\u5404\u7a2e\u4e0a\u76ae\u7d30\u80de\uff08\u539f\u767c\u6027\u524d\u5217\u817a\uff0c\u4e73\u817a\uff0c\u6c23\u9053\u7b49\uff09\u3002<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"HOT\"\u00a0\u00a0<\/u>\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c<\/span><\/a>\u00a0|<\/strong>\u00a0DAPI\u67d3\u6838<\/a>\u00a0|<\/strong>\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291<\/a>\u00a0|<\/strong>\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291<\/a>\u00a0|<\/strong>\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49\u7cfb\u5217\u5546\u54c1<\/a><\/span><\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u00a0TLR9\u6572\u9664\u4e4b\u6c38\u751f\u5316\u5c0f\u9f20\u6a39\u7a81\u72c0\u7d30\u80de | \u8ca8\u865fT3035 TLR9 Knockout Immortalized […]<\/p>\n","protected":false},"author":3,"featured_media":19833,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[453],"tags":[3197],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14032"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=14032"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14032\/revisions"}],"predecessor-version":[{"id":14037,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14032\/revisions\/14037"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/19833"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=14032"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=14032"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=14032"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}