{"id":13887,"date":"2017-09-14T12:45:57","date_gmt":"2017-09-14T04:45:57","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=13887"},"modified":"2022-07-21T10:42:28","modified_gmt":"2022-07-21T02:42:28","slug":"%e8%83%b0%e5%b3%b6%e7%b4%a0%ef%bc%88%e4%ba%ba%ef%bc%89%e6%aa%a2%e6%b8%ac-%e8%b2%a8%e8%99%9fk4742","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=13887","title":{"rendered":"\u80f0\u5cf6\u7d20\uff08\u4eba\uff09\u6aa2\u6e2c | Insulin ELISA Kit \u8ca8\u865fK4742"},"content":{"rendered":"

\u80f0\u5cf6\u7d20\uff08\u4eba\uff09ELISA\u8a66\u5291\u76d2 <\/strong>\u00a0| \u8ca8\u865fK4742<\/a><\/span><\/p>\n

Sensitive, Colorimetric Assay<\/em><\/strong><\/p>\n

\u7522\u54c1\u63cf\u8ff0<\/strong>: <\/strong>BioVision\u7684\u4eba\u80f0\u5cf6\u7d20ELISA\uff08\u9176\u806f\u514d\u75ab\u5438\u9644\u6e2c\u5b9a\uff09\u8a66\u5291\u76d2\u662f\u7528\u65bc\u4eba\u80f0\u5cf6\u7d20\u548c\u80f0\u5cf6\u7d20\u539f\u7684\u5b9a\u91cf\u6e2c\u5b9a\u7684\u9ad4\u5916\u9176\u806f\u514d\u75ab\u5438\u9644\u6e2c\u5b9a\u6cd5\u3002\u8a72\u6e2c\u5b9a\u6cd5\u4f7f\u7528\u572896\u5b54\u677f\u4e0a\u5857\u8986\u4eba\u80f0\u5cf6\u7d20\u7279\u7570\u6027\u6297\u9ad4\u3002\u5c07\u6a19\u6e96\u54c1\u548c\u6a23\u54c1\u79fb\u6db2\u5230\u5b54\u4e2d\uff0c\u6a23\u54c1\u4e2d\u5b58\u5728\u7684\u80f0\u5cf6\u7d20\u901a\u904e\u56fa\u5b9a\u5316\u6297\u9ad4\u8207\u5b54\u7d50\u5408\u3002\u6d17\u6ecc\u5b54\u4e26\u52a0\u5165biotinylated\u7684\u6297\u4eba\u80f0\u5cf6\u7d20\u6297\u9ad4\u3002\u6d17\u53bb\u672a\u7d50\u5408\u7684\u751f\u7269\u7d20\u5316\u6297\u9ad4\u5f8c\uff0c\u5c07HRP\u7db4\u5408\u7684\u93c8\u9ef4\u6297\u751f\u7269\u7d20\u86cb\u767d\u5438\u79fb\u5230\u5b54\u4e2d\u3002\u518d\u6b21\u6c96\u6d17\u5b54\u76e4\uff0c\u5c07a B\u57fa\u8cea\u6eb6\u6db2\u52a0\u5165\u5230\u5b54\u4e2d\uff0c\u4e26\u8207\u80f0\u5cf6\u7d20\u7d50\u5408\u91cf\u6210\u6bd4\u4f8b\u5730\u986f\u8272\u3002\u505c\u6b62\u6eb6\u6db2\u5c07\u984f\u8272\u5f9e\u85cd\u8272\u8b8a\u70ba\u9ec3\u8272\uff0c\u4e26\u4e14\u5728450nm\u8655\u6e2c\u91cf\u984f\u8272\u4e4b\u5f37\u5ea6\u3002\u80f0\u5cf6\u7d20\u7684\u6700\u5c0f\u53ef\u6aa2\u6e2c\u5291\u91cf\u901a\u5e38\u5c0f\u65bc4\u03bcIU\/ ml\u3002\u5206\u6790\u5167\u91cd\u73fe\u6027\u70baCV <10\uff05\uff0c\u5206\u6790\u9593CV <12\uff05\u3002\u8a72ELISA\u8a66\u5291\u76d2\u8207\u4efb\u4f55\u6e2c\u8a66\u7684\u7d30\u80de\u56e0\u5b50\uff08\u4f8b\u5982\u4ebaBDNF\uff0cBLC\uff0cENA-78\uff0cIL-1\u03b1\uff0cIL-1\u03b2\uff0cIL-2\uff0cIL-3\uff0cIL-5\uff0cIL-6\uff0c IL-7\uff0cIL-8\uff0cIL-9\uff0cIL-11\uff0cIL-12 p70\uff0cIL-12p40\uff0cIL-13\uff0cIL-15\uff0cIL-309\uff0cIP-10\uff0cG-CSF\uff0cGM-CSF\uff0c IFN-\u03b3\uff0c\u7626\u7d20\uff08OB\uff09\uff0cMCP-1\uff0cMCP-2\uff0cMCP-3\uff0cMDC\uff0cMIP-1\u03b1\uff0cMIP-1\u03b2\uff0cMIP-1\uff0cPARC\uff0cPDGF\uff0cRANTES\uff0cSCF\uff0cTARC\uff0cTGF-\u03b2\uff0c -1\uff0cTNF-\u03b1\uff0cTNF-\u03b2\uff0cTPO\uff0cVEGF\uff09\u00a0no cross-reactivity\u00a0\u3002<\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK4742-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (450 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nhuman<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nN\/A<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Easy, convenient and time-saving method to assay for human Insulin & Proinsulin.
\n\u2022 The minimum detectable dose of insulin is typically less than 4 \u03bcIU\/ml.
\n\u2022 This ELISA kit is specific for the measurement of human insulin. The kit shows no cross-reactivity with any of the cytokines tested (e.g., human BDNF, BLC, ENA-78, IL-1alpha, IL-1\u03b2, IL-2, IL-3, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-\u03b3, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1alpha, MIP-1\u03b2, MIP-1, PARC, PDGF, RANTES, SCF, TARC, TGF-\u03b2, TIMP-1, TNF-alpha, TNF-\u03b2, TPO, VEGF).<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Insulin Microplate: 96 wells (12 strips x 8 wells) coated with anti-human Insulin.
\n\u2022 Wash Buffer Concentrate (20x): 25 ml of 20x concentrated solution.
\n\u2022 Standards: 2 vials, recombinant human Insulin.
\n\u2022 Assay Diluent A: 30 ml of diluent buffer with 0.09% sodium azide as preservative. For Standard\/Sample (serum\/plasma) diluent.
\n\u2022 Assay Diluent B: 15 ml of 5x concentrated buffer. For Standard\/Sample (cell culture medium\/urine) diluent.
\n\u2022 Detection Antibody Insulin: 2 vial of biotinylated anti-human Insulin (each vial is enough to assay half microplate).
\n\u2022 HRP-Streptavidin Concentrate: 200 \u00b5l of 500x concentrated HRP-conjugated streptavidin.
\n\u2022 \u2122B One-Step Substrate Reagent: 12 ml of 3,3\u2019,5,5\u2019tetramethylbenzidine (\u2122B) in buffered solution.
\n\u2022 Stop Solution: 8 ml of 0.2 M sulfuric acid.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u76f8\u95dc\u8a66\u5291\"\u00a0\u4e09\u9178\u7518\u6cb9\u916f\u5206\u6790\u5957\u7d44<\/strong> <\/a>| <\/strong>\u80c6\u56fa\u9187\u5206\u6790<\/strong><\/a>\u00a0| <\/strong>\u8102\u8cea\u7d30\u80de\u67d3\u8272\u5291<\/strong><\/a> | <\/strong>\u9577\u93c8\u6e38\u96e2\u8102\u80aa\u9178\u5206\u6790\u5957\u7d44<\/strong><\/a> | <\/strong>TG ELISA<\/strong>\u5957\u7d44<\/strong><\/a><\/p>\n

\"\u76f8\u95dc\u5100\u5668\"\u00a0\u9175\u7d20\u514d\u75ab\u5206\u6790\u5100<\/strong>\u00a0<\/a>\u00a0|\u00a0\u5fae\u91cf\u76e4\u6e05\u6d17\u6a5f<\/a>\u00a0 |\u00a0\u5fae\u91cf\u76e4\u9707\u76ea\u57f9\u990a\u7bb1<\/a><\/strong><\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u80f0\u5cf6\u7d20\uff08\u4eba\uff09ELISA\u8a66\u5291\u76d2 \u00a0| \u8ca8\u865fK4742 Sensitive, Colorimetric Assay […]<\/p>\n","protected":false},"author":3,"featured_media":6324,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[180,1],"tags":[3209],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13887"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=13887"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13887\/revisions"}],"predecessor-version":[{"id":13901,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13887\/revisions\/13901"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6324"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=13887"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=13887"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=13887"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}