{"id":13797,"date":"2017-09-08T11:28:00","date_gmt":"2017-09-08T03:28:00","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=13797"},"modified":"2025-03-21T14:51:21","modified_gmt":"2025-03-21T06:51:21","slug":"%e6%b0%b8%e7%94%9f%e5%8c%96%e5%b0%8f%e9%bc%a0%e8%82%9d%e6%ad%a3%e4%b8%ad%e5%85%a7%e7%9a%ae%e7%b4%b0%e8%83%9e-sv40-hepatic-sinusoidal-endothelial-cells-sv40%e8%b2%a8%e8%99%9ft0102","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=13797","title":{"rendered":"\u6c38\u751f\u5316\u5c0f\u9f20\u809d\u7ac7\u5167\u76ae\u7d30\u80de-SV40 | Hepatic Sinusoidal Endothelial Cells- SV40\u8ca8\u865fT0102"},"content":{"rendered":"

\u7d30\u80de\u7cfb\u5217\u5546\u54c1<\/strong> (immortalized cell)-LSEC<\/strong><\/span><\/p>\n

\u6c38\u751f\u5316\u5c0f\u9f20\u809d\u7ac7\u5167\u76ae\u7d30\u80de-SV40 <\/strong>(<\/strong>\u8ca8\u865f<\/strong>T0102)\u00a0<\/strong>Hepatic Sinusoidal Endothelial Cells- SV40<\/a><\/span><\/p>\n

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\u809d\u7ac7\u5167\u76ae\u7d30\u80de\uff08LSEC, Liver sinusoidal endothelial cells\uff09\u5728\u809d\u7ac7\u548c\u5e95\u5c64\u5be6\u8cea\u4e4b\u9593\u5f62\u6210\u7368\u7279\u7684\u5c4f\u969c\uff0c\u56e0\u6b64\u5728\u7dad\u6301\u4ee3\u8b1d\u548c\u514d\u75ab\u7a69\u614b\u4ee5\u53ca\u7a4d\u6975\u4fc3\u9032\u75be\u75c5\u75c5\u7406\u751f\u7406\u5b78\u65b9\u9762\u767c\u63ee\u81f3\u95dc\u91cd\u8981\u7684\u4f5c\u7528\u3002\u809d\u7ac7\u5167\u76ae\u7d30\u80de\uff08LSEC, Liver sinusoidal endothelial cells\uff09\u662f\u9ad8\u5ea6\u7279\u5316\u7684\u5167\u76ae\u7d30\u80de\uff0c\u4ee3\u8868\u4e00\u5074\u7684\u8840\u7403\u8207\u53e6\u4e00\u5074\u7684\u809d\u7d30\u80de\u548c\u809d\u661f\u72c0\u7d30\u80de\u4e4b\u9593\u7684\u754c\u9762\u3002\u809d\u7ac7\u5167\u76ae\u7d30\u80de\uff08LSEC, Liver sinusoidal endothelial cells\uff09\u662f\u4e00\u7a2e\u7368\u7279\u7684\u809d\u81df\u4e9e\u7fa4\uff0c\u809d\u81dfsinusoids\u3002\u5b83\u5011\u7684\u4f5c\u7528\u6d89\u53ca\u6e05\u9664\u8840\u6db2\u4e2d\u7684\u5ee2\u7269\uff0c\u8abf\u7bc0\u5468\u908a\u7d30\u80de\u7684\u6536\u7e2e\u6027\u548c\u5148\u5929\u7684\u514d\u75ab\u529f\u80fd\u3002\u6c38\u751f\u5316\u7684\u5c0f\u9f20Hepatic Sinusoidal Endothelial Cells- SV40\u4f86\u81ea\u9f20HSECs\uff0c\u5176\u4fdd\u6301\u5167\u76ae\u7279\u5fb5\u548c\uff08LSEC, Liver sinusoidal endothelial cells\uff09\u7279\u7570\u6027\u7279\u5fb5\uff0c\u4f8b\u5982vWF\u548ccaveolin -1\u3002 \u9019\u4e9b\u7d30\u80de\u4e5f\u97ff\u61c9\u65bc\u8840\u7ba1\u751f\u6210\u56e0\u5b50\uff0c\u5f62\u6210\u8840\u7ba1\u7ba1\u72c0\u7d50\u69cb\uff0c\u5167\u5207\u7d30\u80de\u4e59\u9170\u5316\u4f4e\u5bc6\u5ea6\u8102\u86cb\u767d\uff08endycytose acetylated low-density lipoprotein , AcLDL\uff09\u548c\u5206\u6ccc\u86cb\u767d\u8cea\u6d89\u53ca\u57fa\u8cea\u91cd\u5851(remodelling)\u3002 \u6b64\u5916\uff0c\u9019\u4e9b\u7d30\u80de\u5bb9\u6613\u88abretrovirus\u9006\u8f49\u9304\u75c5\u6bd2\u8f49\u5c0e\u3002 \u6c38\u751f\u5316Mouse Hepatic Sinusoidal Endothelial Cells- SV40\u56e0\u6b64\u9069\u7528\u65bc\u7814\u7a76\u809d\u5167\u76ae\u7d30\u80de\u751f\u7269\u5b78\uff0c\u5305\u62ec\u904b\u52d5\u548c\u8840\u7ba1\u751f\u6210\u4e4b\u7814\u7a76\u3002<\/p>\n

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\u6c38\u751f\u5316\u5c0f\u9f20\u809d\u7ac7\u5167\u76ae\u7d30\u80de-SV40\u7279\u6027<\/strong><\/p>\n

1.\u80fd\u5920\u541e\u566cAcLDL;<\/p>\n

2.\u589e\u52a0\u5c0dVEGF\u548cFGF\u7684\u9077\u79fb\u7387; \u80fd\u5f62\u6210\u5f37\u58ef\u7684\u8840\u7ba1\u7ba1\u6a23\u7d50\u69cb;<\/p>\n

4.\u9006\u8f49\u9304\u75c5\u6bd2\u7684\u8f49\u5c0e\u6548\u7387\u70ba90\uff05;<\/p>\n

5.\u8010\u8840\u6e05\u98e2\u9913\u3002<\/p>\n

<\/p>\n\n\n\n\n\n\n\n\n\n\n
BioSafety Level<\/strong><\/td>\nII<\/td>\n<\/tr>\n
Organism<\/strong><\/td>\nMus musculus<\/td>\n<\/tr>\n
Source Organ<\/strong><\/td>\nLiver<\/td>\n<\/tr>\n
Growth Properties<\/strong><\/td>\nAdherent<\/td>\n<\/tr>\n
Morphology<\/strong><\/td>\nCobblestone-like<\/td>\n<\/tr>\n
Population Doubling<\/strong><\/td>\n22-32 hours<\/td>\n<\/tr>\n
Recommended Seeding Density<\/strong><\/td>\n5,000-7,000 cells\/cm2<\/sup><\/td>\n<\/tr>\n
Markers<\/strong><\/td>\nvWF and caveolin-1<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5c0f\u9f20\u809d\u809d\u7ac7\u5167\u76ae\u7d30\u80de-SV40 <\/strong>\u7d30\u80de\u7279\u6027: Adherent \/ Cobblestone-like | \u4f86\u6e90Liver\u3002\u7d30\u80de\u57f9\u990a\u689d\u4ef6: \u4f7f\u7528PriCoat\u2122T25\u57f9\u990a\u74f6 (\u8ca8\u865fG299<\/a>) \u4ee5\u6700\u4f73\u5316\u521d\u4ee3\u548c\u4e0d\u673d\u7d30\u80de\u7684\u751f\u9577\u3001\u589e\u6b96\u548c\u64f4\u589e\u3002\u6216\u5857\u5c64\u7d30\u80de\u5916\u57fa\u8cea(Applied Cell Extracellular Matrix) \u589e\u5f37\u7d30\u80de\u8cbc\u9644 (\u8ca8\u865fG422<\/a>)\u3002Grow cells in ECM-coated culture vessels with the following conditions. Complete growth medium\u57f9\u990a\u6db2PriGrowI (\u8ca8\u865fTM001<\/a>\u00a0)\uff0c\u80ce\u725b\u8840\u6e05(Cat. No. TM999<\/a>)\u6700\u7d42\u6fc3\u5ea6\u70ba10%\u4e26\u586b\u52a01% Penicillin\/Streptomycin \u30021%. Atmosphere: air, 95%; Carbon dioxide (CO2), 5%. Temperature: 37.0\u00b0C<\/p>\n

<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat. No.<\/td>\nT0102<\/span><\/td>\n<\/tr>\n
Name<\/td>\nImmortalized Mouse Hepatic Sinusoidal Endothelial Cells – SV40<\/td>\n<\/tr>\n
Description<\/td>\nHepatic sinusoidal endothelial cells (HSECs) are an unique sub-population of liver cells that line the hepatic sinusoids. Their role involves clearing waste products from the blood, regulating pericyte contractility and innate immune functions. The Immortalized Mouse Hepatic Sinusoidal Endothelial Cells – SV40 is derived from C57BL\/6 mouse HSECs that have maintained endothelial characteristics and HSEC-specific features such as vWF and caveolin-1. These cells also migrate in response to angiogenici growth factors, form vascular tube-like structures, endycytose acetylated low-density lipoprotein (AcLDL) and secrete proteins involved in matrix remodelling. In addition, these cells are easily transduced by retrovirus. The Immortalized Mouse Hepatic Sinusoidal Endothelial Cells- SV40 is therefore suitable for studying liver endothelial cell biology, including motility and angiogenesis.<\/td>\n<\/tr>\n
Organism<\/td>\nMouse (M. musculus)<\/td>\n<\/tr>\n
Tissue<\/td>\nLiver<\/td>\n<\/tr>\n
Donor History<\/td>\nC57BL\/6 mouse<\/td>\n<\/tr>\n
Growth Properties<\/td>\nAdherent, polygonal<\/td>\n<\/tr>\n
Cell Type<\/td>\nImmortalized Cells<\/td>\n<\/tr>\n
Storage Condition<\/td>\nVapor phase of liquid nitrogen, or below -130\u00b0C.<\/td>\n<\/tr>\n
Shipping Conditions<\/td>\nShip with dry ice.<\/td>\n<\/tr>\n
Product Format<\/td>\nFrozen<\/td>\n<\/tr>\n
Intended Use<\/td>\nThis product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.<\/td>\n<\/tr>\n
BioSafety<\/td>\nII<\/td>\n<\/tr>\n
Certificate of Analysis<\/td>\nFor batch-specific test results, refer to the applicable certificate of analysis that can be found at www.abmgood.com.<\/td>\n<\/tr>\n
Growth Conditions<\/td>\nUse of PriCoat\u2122 T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow I (TM001) + 5% FBS + 1% Penicillin\/Streptomycin Solution (G255), 37.0\u00b0C, 5% CO\u2082<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

<\/p>\n

HOT!!<\/strong><\/span>\u00a0<\/u>\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c<\/a>\u00a0|<\/strong>\u00a0DAPI\u67d3\u6838<\/a>\u00a0|<\/strong>\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291<\/a>\u00a0|<\/strong>\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291<\/a>\u00a0|<\/strong>\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49\u7cfb\u5217\u5546\u54c1<\/a><\/span><\/p>\n

Unpacking and Storage Instructions<\/p>\n

1. Visually examine the packaging containers for signs of leakage or breakage.<\/p>\n

2. Immediately transfer frozen cells from dry ice packaging to a temperature below -130\u00b0C, preferably in liquid nitrogen vapor phase storage, until ready for use.<\/p>\n

To ensure the highest level of viability, thaw the vial and initiate culture as soon as possible upon receipt. If continued storage is desired, the vial should only be stored below -130\u00b0C or in liquid nitrogen vapor phase. Do not store at -70\u00b0C, as it will result in loss of viability.<\/p>\n

Thawing Protocol<\/p>\n

1. Thaw cells quickly in a 37\u00b0C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.<\/p>\n

2. Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.<\/p>\n

3. Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes.<\/p>\n

4. Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask.<\/p>\n

5. Incubate the cells at the recommended conditions.<\/p>\n

Subculture Protocol<\/p>\n

Volumes given below are for a T75 flask; proportionally increase or decrease the volume as required per culture vessel size. Subculture cells once the culture vessel is 80% confluent.<\/p>\n

1. Aspirate the culture media, and add 2-3ml of pre-warmed 0.25% Trypsin-EDTA to the culture vessel.<\/p>\n

2. Observe the cells under a microscope to confirm detachment (typically within 2-10 minutes). Cells that are difficult to detach can be put in 37\u00b0C, for several minutes to facilitate detachment.<\/p>\n

3. Neutralize Trypsin-EDTA by adding an equal volume of the complete growth media into the culture vessel.<\/p>\n

4. Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 125xg for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type.<\/p>\n

5. Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Add appropriate aliquots of the cell suspension to new culture vessels, as desired.<\/p>\n

6. Incubate the cells at the recommended conditions.<\/p>\n

Cryopreservation<\/p>\n

Cryopreservation Medium (TM024), or complete growth media with 10% DMSO.<\/p>\n

Seeding Density (cells\/cm2<\/sup>)5,000 – 7,000Split Ratio1:2 to 1:3Population Doubling Time (h)22 – 32Immortalization MethodSerial passaging and transduction with pantropic recombinant retroviruses carrying SV40 Large T antigenExpression<\/p>\n

vWF and caveolin-1<\/p>\n

Material CitationIf use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. T0102.<\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u7d30\u80de\u7cfb\u5217\u5546\u54c1 (immortalized cell)-LSEC \u6c38\u751f\u5316\u5c0f\u9f20\u809d\u7ac7\u5167\u76ae\u7d30\u80de-SV40 (\u8ca8\u865fT01 […]<\/p>\n","protected":false},"author":3,"featured_media":13798,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5373,453],"tags":[3216],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13797"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=13797"}],"version-history":[{"count":9,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13797\/revisions"}],"predecessor-version":[{"id":50839,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13797\/revisions\/50839"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/13798"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=13797"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=13797"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=13797"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}