{"id":13482,"date":"2017-08-31T11:25:02","date_gmt":"2017-08-31T03:25:02","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=13482"},"modified":"2022-07-21T10:46:34","modified_gmt":"2022-07-21T02:46:34","slug":"protease-k%e8%9b%8b%e7%99%bd%e9%85%b6k-%e8%b2%a8%e8%99%9f9212","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=13482","title":{"rendered":"Protease K\/\u86cb\u767d\u9176K | \u8ca8\u865f9212"},"content":{"rendered":"<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=13482\" target=\"_blank\">Protease K\/\u86cb\u767d\u9176K <strong>| <\/strong><strong>\u8ca8\u865f<\/strong>9212<\/a><\/span><\/p>\n<p><strong><em>FabAct\u2122 Proteinase K (recombinant), Molecular Grade (Solid)<\/em><\/strong><\/p>\n<p><strong>\u7522\u54c1\u4ecb\u7d39<\/strong><strong>:<\/strong> BioVision\u81ea\u63a8\u51faMolecular Biology Grade\u5206\u5b50\u751f\u7269\u5b78\u7d1a\u5225\uff0cFabulous\u6d3b\u6027\uff08FabActTM\uff09\u4e4bProteinase K\u86cb\u767d\u9176K\uff08\u91cd\u7d44\uff09\uff0cProteinase K \u81f3\u4eca\u662f\u6240\u6709\u86cb\u767d\u9176\u4e2d\u6700\u5f37\u5927\u7684proteinases\u86cb\u767d\u9176\u3002 FabActTM\u86cb\u767d\u9176K\u662f\u4e00\u7a2e\u9ad8\u7d14\u5ea6\uff0c\u9ad8\u53cd\u61c9\u6027\u5177\u7d72\u6c28\u9178\u86cb\u767d\u9176\u6d88\u5316\u5929\u7136\u86cb\u767d\u8cea\u7684\u80fd\u529b\uff0c\u5f9e\u800c\u4f7f\u9176\u5982DNase\u548cRNase\u5931\u6d3b\u3002 \u5b83\u8207\u9130\u8fd1\u7fa7\u9178\u7684\u80bd\u9375\u65b7\u88c2, \u5982aliphatic\u8102\u80aa\u65cf\uff0caromatic\u82b3\u9999\u65cf\u6216hydrophobic amino acids\u758f\u6c34\u6027\u6c28\u57fa\u9178\u3002 FabActTM\u86cb\u767d\u9176K\u5177\u6709\u8f03\u9ad8\u7684\u7279\u7570\u6027\u6d3b\u6027\uff0c\u4e26\u4e14\u8207\u5929\u7136\u86cb\u767d\u9176K\u76f8\u6bd4\u5728\u5ba4\u6eab\u4e0b\u66f4\u5177\u7a69\u5b9a\u6027\u3002\u5728pH4-12\u5bec\u7684pH\u7bc4\u570d\u5167\u5177\u6d3b\u8e8d\u53cd\u61c9\u3002FabAct\u53ef\u61c9\u7528\u65bc\u4efb\u4f55\u60c5\u6cc1\u4e0b\u6d88\u5316native\u53ca denatured\u86cb\u767d\u8cea\u3002\u4e0d\u6d3b\u5316\u4e8c\u7570\u4e19\u57fa\u6c1f\u78f7\u9178\u916f\uff08DFP\uff09\u548c\u82ef\u57fa\u7532\u78fa\u9170\u6c1f\uff08PMSF\uff09\u3002FabActTM\u86cb\u767d\u9176K\u5728SDS\uff0c\u5c3f\u7d20\u548cEDTA\u4e0b\u4e5f\u5177\u6709\u6d3b\u6027, \u5176\u6700\u9ad8\u6d3b\u6027\u6eab\u5ea6\u70ba65\u00b0C\u3002<\/p>\n<p><img decoding=\"async\" src=\"http:\/\/www.biovision.com\/media\/catalog\/product\/cache\/1\/image\/650x\/040ec09b1e35df139433887a97daa66f\/9\/2\/9212_1.jpg\" alt=\"\" \/><\/p>\n<p>Proteinase K\u61c9\u7528\uff1a<\/p>\n<ul>\n<li>\u5f9e\u7d44\u7e54\u6216\u7d30\u80de\u7cfb\u4e2d\u5206\u96e2DNA\u548cRNA\u6642\uff0c\u53bb\u9664DNA\u548cRNA\u9176\u61c9\u7528\u65bc\u64f4\u589e\u53cd\u61c9<\/li>\n<li>\u5f9e\u5305\u62ec\u5c0f\u9f20\u5c3e\u5df4\u6216\u57f9\u990a\u7684\u4e0d\u540c\u7d44\u7e54\u7d30\u80de\u5206\u96e2mRNA\u6216\u57fa\u56e0\u7d44DNA<\/li>\n<li>\u7528\u65bc\u4fee\u98fe\u7cd6\u86cb\u767d\u9032\u884c\u7d50\u69cb\u7814\u7a76<\/li>\n<li>\u6cbb\u7642\u7d44\u7e54\u5207\u7247\u9032\u884c\u539f\u4f4d\u96dc\u4ea4<\/li>\n<li>\u63d0\u9ad8PCR\u7522\u7269\u7684\u514b\u9686\u6548\u7387<\/li>\n<li>\u70ba\u4e86\u5206\u96e2\u751f\u7269\u7522\u54c1\u4ee5\u6d88\u9664\u884c\u696d\u4e2d\u7684\u86cb\u767d\u8cea\u6c61\u67d3\u7269\uff0c\u5982\u76ae\u9769\uff0c\u98df\u54c1\uff0c\u91ab\u85e5\u4e2d\u9593\u9ad4\u7b49<\/li>\n<\/ul>\n<table width=\"838\">\n<tbody>\n<tr>\n<td><strong>Alternate Name<\/strong><\/td>\n<td>Protease K, Endopeptidase K, Tritirachium alkaline proteinase<\/td>\n<\/tr>\n<tr>\n<td><strong>Gene Symbol<\/strong><\/td>\n<td>PROK<\/td>\n<\/tr>\n<tr>\n<td><strong>Gene ID<\/strong><\/td>\n<td>5621<\/td>\n<\/tr>\n<tr>\n<td><strong>Accession #<\/strong><\/td>\n<td>P06873<\/td>\n<\/tr>\n<tr>\n<td><strong>Source<\/strong><\/td>\n<td>Tritirachium album<\/td>\n<\/tr>\n<tr>\n<td><strong>Appearance<\/strong><\/td>\n<td>Lyophilized<\/td>\n<\/tr>\n<tr>\n<td><strong>Physical Form Description<\/strong><\/td>\n<td>Lyophilized with no additives<\/td>\n<\/tr>\n<tr>\n<td><strong>Molecular Weight<\/strong><\/td>\n<td>28.9 kDa<\/td>\n<\/tr>\n<tr>\n<td><strong>Purity by SDS-PAGE<\/strong><\/td>\n<td>\u226590%<\/td>\n<\/tr>\n<tr>\n<td><strong>Reconstitution Instructions<\/strong><\/td>\n<td>In 50 mM Tris-HCl, 2 mM Calcium acetate, pH 8.0.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><span style=\"color: #ff0000;\">\u86cb\u767d\u8cea\u5b9a\u91cf\u5546\u54c1<\/span> <span style=\"color: #3366ff;\"><a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=9811\">Coomassie blue\u86cb\u767d\u8cea\u5b9a\u91cf\u5206\u6790\u5957\u7d44<\/a> | <a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=10036\">BCA\u86cb\u767d\u6e2c\u5b9a\u8a66\u5291\u76d2(\u4f4e\u6fc3\u5ea610-600ug\/ml)| 5\uff05\u8010\u6d17\u6ecc <\/a>| <a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=10023\">BCA\u86cb\u767d\u6e2c\u5b9a\u8a66\u5291\u76d2 (\u9084\u539f\u5291\u517c\u5bb9)<\/a><\/span><\/p>\n<table style=\"height: 660px;\" width=\"610\">\n<tbody>\n<tr>\n<td width=\"111\">\u6291\u5236\u5291\u8ca8\u865f<\/td>\n<td width=\"198\">\u6291\u5236\u5291\u54c1\u9805<\/td>\n<td width=\"302\">\u86cb\u767d\u9176\/\u78f7\u9178\u9176\u6291\u5236\u7a2e\u985e<\/td>\n<\/tr>\n<tr>\n<td rowspan=\"4\" width=\"111\">K271-500<\/td>\n<td rowspan=\"4\" width=\"198\"><span style=\"color: #3366ff;\"><a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=13482\">\u86cb\u767d\u9176\u6291\u5236\u5291<em>Cocktail<\/em><\/a><\/span><\/td>\n<td width=\"302\"><em>Aspartic\/<\/em><\/td>\n<\/tr>\n<tr>\n<td width=\"302\"><em>Cysteine<\/em><\/td>\n<\/tr>\n<tr>\n<td width=\"302\"><em>Serine proteases<\/em><\/td>\n<\/tr>\n<tr>\n<td width=\"302\"><em>Esterases<\/em><\/td>\n<\/tr>\n<tr>\n<td rowspan=\"4\" width=\"111\">K272-set<\/td>\n<td rowspan=\"4\" width=\"198\"><span style=\"color: #3366ff;\"><a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=13492\">EZBlock\u2122\u86cb\u767d\u9176\u6291\u5236\u5291Cocktails\uff0c\u4e0d\u542bEDTA<\/a><\/span><\/td>\n<td width=\"302\">serine, ,\u7d72\u6c28\u9178<\/td>\n<\/tr>\n<tr>\n<td width=\"302\">cysteine\u534a\u80f1\u6c28\u9178<\/td>\n<\/tr>\n<tr>\n<td width=\"302\">aspartic proteases\u5929\u51ac\u6c28\u9178\u86cb\u767d\u9176<\/td>\n<\/tr>\n<tr>\n<td width=\"302\">aminopeptidases\u6c28\u57fa\u80bd\u9176<\/td>\n<\/tr>\n<tr>\n<td rowspan=\"5\" width=\"111\">K277-set<\/td>\n<td rowspan=\"5\" width=\"198\"><span style=\"color: #3366ff;\"><a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=13497\">EZBlock\u2122\u86cb\u767d\u9176\u6291\u5236\u5291Cocktail II<\/a><\/span><\/td>\n<td width=\"302\">Serine proteases<\/td>\n<\/tr>\n<tr>\n<td width=\"302\">Broad spectrum, serine proteases, esterases<\/td>\n<\/tr>\n<tr>\n<td width=\"302\">Cysteine Proteases<\/td>\n<\/tr>\n<tr>\n<td width=\"302\">Metalloproteases<\/td>\n<\/tr>\n<tr>\n<td width=\"302\">Serine\/Cysteine proteases, Trypsinlike proteases<\/td>\n<\/tr>\n<tr>\n<td rowspan=\"7\" width=\"111\">K394-set<\/td>\n<td rowspan=\"7\" width=\"198\"><span style=\"color: #3366ff;\"><a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=13503\">EZBlock\u2122\u86cb\u767d\u9176\u6291\u5236\u5291Cocktail II (Animal-Free)<\/a><\/span><\/td>\n<td width=\"302\"><em>Serine proeinase<\/em><\/td>\n<\/tr>\n<tr>\n<td width=\"302\"><em>Serine proteases\/<\/em><\/td>\n<\/tr>\n<tr>\n<td width=\"302\"><em>Cysteine Proteases<\/em><\/td>\n<\/tr>\n<tr>\n<td width=\"302\"><em>Serine<\/em><\/td>\n<\/tr>\n<tr>\n<td width=\"302\"><em>HIV proteasees,<\/em><\/td>\n<\/tr>\n<tr>\n<td width=\"302\"><em>Aspartic proteases,<\/em><\/td>\n<\/tr>\n<tr>\n<td width=\"302\"><em>Acid proteases<\/em><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Protease K\/\u86cb\u767d\u9176K | \u8ca8\u865f9212 FabAct\u2122 Proteinase K (recombin [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":6512,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[134],"tags":[3258],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13482"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=13482"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13482\/revisions"}],"predecessor-version":[{"id":13522,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13482\/revisions\/13522"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6512"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=13482"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=13482"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=13482"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}