{"id":13369,"date":"2017-08-29T09:47:33","date_gmt":"2017-08-29T01:47:33","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=13369"},"modified":"2022-07-21T11:11:55","modified_gmt":"2022-07-21T03:11:55","slug":"%e7%b8%bd%e8%bd%89%e9%8c%84%e7%b5%84cdna%e5%90%88%e6%88%90%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fg905","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=13369","title":{"rendered":"\u7e3d\u8f49\u9304\u7d44cDNA\u5408\u6210\u8a66\u5291\u76d2 | \u8ca8\u865fG905"},"content":{"rendered":"<p><a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=10086\"><strong>\u7e3d\u8f49\u9304\u7d44cDNA\u5408\u6210\u8a66\u5291\u76d2<\/strong><strong>\u00a0|\u00a0<\/strong><strong>\u8ca8\u865f<\/strong><strong>G905 100\u500b\u53cd\u61c9<\/strong><\/a><\/p>\n<p>Abmgood\u63d0\u4f9b\u4e86\u4e00\u7cfb\u5217RNA\u548cDNA\u7d14\u5316\u8a66\u5291\u76d2\uff0c\u53ef\u4ee5\u7522\u751f\u9ad8\u8cea\u91cfRNA\u548cDNA\uff0c\u9069\u5408\u65bc\u4e0b\u6e38\u5982sequencing\u5b9a\u5e8f\u3001PCR\u3001\u9650\u5236\u6027\u5207\u5272\u8207\u5206\u5b50\u9023\u63a5\u3002\u7522\u54c1\u8a2d\u8a08\u539f\u7406\u70basilica spin column\u65cb\u8f49\u67f1\u7d14\u5316\u65b9\u6cd5\uff0c\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u55ae\u5feb\u901f\uff0c\u66ff\u4ee3\u50b3\u7d71\uff08\u4e59\u9187\u6c89\u6fb1\uff09\u548c\u4e0d\u9700\u8981\u6bd2\u6027\u915a\/\u6c2f\u4eff\u8403\u53d6\u6216\u7e41\u7463\u7684CsCl\u68af\u5ea6\uff0cLiCl\u6216\u4e59\u9187\u6c89\u6fb1\u6280\u8853\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/www.abmgood.com\/PCR\/images\/Total-Transcriptome_cDNA_Synthesis_kit-G904-G905.jpg\" alt=\"\" width=\"511\" height=\"381\" \/><\/p>\n<p><strong>\u7522\u54c1\u4ecb\u7d39:\u00a0<\/strong>\u00a0abm\u7684\u7e3d\u8f49\u9304\u7d44cDNA\u5408\u6210\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u5728\u6a23\u54c1\uff08\u5982mRNA\uff0cmiRNA\u548clncRNA\uff09\u4e2d\u5f9e\u6240\u6709\u4e0d\u540c\u7a2e\u985e\u7684RNA\u7522\u751f\u9ad8\u8cea\u91cfcDNA\u6240\u9700\u7684\u6240\u6709\u8a66\u5291\u3002\u9019\u53ef\u4ee5\u901a\u904e\u4f7f\u7528\u805a\uff08A\uff09\u805a\u5408\u9176\u76843&#8217;\u591a\u805a\uff08A\uff09RNA\u7684RNA\u4f86\u5be6\u73fe\u3002 Oligo d\uff08T\uff09\u929c\u63a5\u5b50\u548c\u96a8\u6a5f\u5f15\u7269\u7136\u5f8c\u9000\u706b\u5230RNA\u6a21\u677f\u4e0a\u4e26\u4fc3\u9032\u9006\u8f49\u9304\u3002 OneScript\u00aePlus\u9006\u8f49\u9304\u9176\u662f\u4e00\u7a2e\u65b0\u578b\u7684\u91cd\u7d44\u9006\u8f49\u9304\u9176\uff0c\u5176\u5728\u5177\u6709\u4e8c\u7d1a\u7d50\u69cb\u548c\u9ad8GC\u542b\u91cf\u7684RNA\u6a21\u677f\u4e2d\u5728\u7b2c\u4e00\u93c8cDNA\u5408\u6210\u4e2d\u8868\u73fe\u51fa\u66f4\u9ad8\u7684\u6548\u7387\u3002 OneScript\u00aePlus\u9006\u8f49\u9304\u9176\u8a2d\u8a08\u7528\u65bc\u5728\u9ad8\u6eab\uff0850\u00b0C\u81f355\u00b0C\uff09\u4e0b\u9032\u884c;\u6709\u52a9\u65bc\u6d88\u9664\u8207\u5bcc\u542bGC\u7684RNA\u6a21\u677f\u76f8\u95dc\u7684\u4e8c\u7d1a\u7d50\u69cb\u3002\u91cd\u7d44RNaseOFF\u6838\u7cd6\u6838\u9178\u9176\u6291\u88fd\u5291\u6709\u6548\u4fdd\u8b77RNA\u6a21\u677f\u4e0d\u88ab\u964d\u89e3\u3002\u6b64\u5916\uff0c\u8a66\u5291\u76d2\u4e2d\u5305\u542b\u7684\u901a\u75283&#8217;\u53cd\u5411\u5f15\u7269\u53ef\u4ee5\u8207\u4e0b\u6e38qPCR \/ PCR\u64f4\u589e\u4e2d\u7684\u4efb\u4f55\u7279\u7570\u6027\u6b63\u5411\u5f15\u7269\u5076\u806f\u3002<strong>\u7522\u54c1\u7279\u9ede:\u00a0<\/strong>\u53ef\u61c9\u7528\u65bc\u4eba\u6216\u5c0f\u9f20RNA\u6a23\u54c1\u4e2d\u7684\u6240\u6709RNA; 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