{"id":13364,"date":"2017-08-28T17:19:42","date_gmt":"2017-08-28T09:19:42","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=13364"},"modified":"2023-09-25T14:16:52","modified_gmt":"2023-09-25T06:16:52","slug":"%e8%84%ab%e8%9b%8b%e7%99%bd%e6%a8%a3%e5%93%81%e8%a3%bd%e5%82%99%e8%a9%a6%e5%8a%91%e7%9b%92-deproteinizing-sample-preparation-kit%e8%99%9fk417","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=13364","title":{"rendered":"PCA\u812b\u86cb\u767d\u6a23\u54c1\u88fd\u5099\u8a66\u5291\u76d2 | Deproteinizing Sample Preparation Kit\u865fK808 (ABCAM, ab284939)"},"content":{"rendered":"<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=13364\" target=\"_blank\" rel=\"noopener\"><strong><span style=\"font-size: 12pt;\">\u812b\u86cb\u767d\u6a23\u54c1\u88fd\u5099\u8a66\u5291\u76d2 <\/span><\/strong><span style=\"font-size: 12pt;\"><strong>| <\/strong><strong>\u8ca8\u865f<\/strong>K8<\/span><\/a><span style=\"font-size: 12pt;\">08 (ABCAM, ab284939)<\/span><\/span><\/p>\n<p><strong><em>Deproteinizing Sample Preparation Kit<\/em><\/strong><\/p>\n<p><strong>\u7522\u54c1\u4ecb\u7d39<\/strong><strong>:<\/strong> \u86cb\u767d\u8cea\u548c\u5404\u7a2e\u9176\u6d3b\u6027\u7684\u5b58\u5728\u7d93\u5e38\u5e72\u64fe\u751f\u7269\u6a23\u54c1\u4e2d\u5c0f\u5206\u5b50\u7684\u5206\u6790\u3002\u8a31\u591a\u751f\u7269\u6e2c\u5b9a\u9700\u8981\u5728\u5206\u6790\u524d\u5f9e\u6a23\u54c1\u4e2d\u53bb\u9664\u86cb\u767d\u8cea\u3002\u5728\u904e\u53bb\u534a\u500b\u4e16\u7d00\u958b\u767c\u7684\u812b\u86cb\u767d\u65b9\u6848\u4e2d\uff0c\u9ad8\u6c2f\u9178\uff08PCA\uff09\u6c89\u6fb1\u5df2\u7d93\u5ee3\u6cdb\u61c9\u7528\u65bc\u8a31\u591a\u4e0d\u540c\u7684\u6a23\u54c1\u88fd\u5099\u7a0b\u5e8f\uff0c\u56e0\u70ba\u5b83\u4e0d\u50c5\u53ef\u4ee5\u53bb\u9664\u5927\u90e8\u5206\u5b58\u5728\u7684\u86cb\u767d\u8cea\uff0c\u800c\u4e14\u9084\u53ef\u4ee5\u7a69\u5b9a\u8a31\u591a\u5c0f\u5206\u5b50\u5206\u6790\u7269\u3002 PCA\u53bb\u86cb\u767d\u5316\u65b9\u6cd5\u5df2\u7d93\u6210\u529f\u61c9\u7528\u65bc\u5b9a\u91cf\u5c0f\u5206\u5b50\u9663\u5217\uff08\u5305\u62ec\u7cd6\u539f\uff0cATP\uff0ccAMP\uff0c\u7a40\u80f1\u7518\u80bd\uff0c\u6297\u6c27\u5316\u5291\u7b49\uff09\u4e4b\u524d\u7684\u6e96\u5099\u6a23\u54c1\u4e2d\u3002\u65b0\u8a2d\u8a08\u7684\u812b\u86cb\u767d\u6a23\u54c1\u88fd\u5099\u8a66\u5291\u76d2\u5229\u7528PCA\u6c89\u6fb1\u6cd5\uff0c\u63d0\u4f9b\u7368\u7279\u7684\u5de5\u5177\u6e96\u5099\u6a23\u54c1\u7528\u65bc\u5206\u6790\u5404\u7a2e\u5c0f\u5206\u5b50\u3002\u9664\u86cb\u767d\u8cea\u6c89\u6fb1\u5916\uff0c\u53bb\u9664\u591a\u9918\u7684PCA\uff0c\u6a23\u54c1\u6700\u5f8c\u88ab\u4e2d\u548c\u3002\u4f7f\u7528\u8a72\u8a66\u5291\u76d2\u88fd\u5099\u7684\u6a23\u54c1\u53ef\u76f4\u63a5\u7528\u65bc\u5404\u7a2e\u751f\u7269\u6e2c\u5b9a\u3002\u8a72\u65b9\u6cd5\u65b9\u4fbf\uff0c\u65b9\u4fbf\uff0c\u53ef\u7528\u65bc\u88fd\u5099\u5927\u91cf\u6a23\u54c1\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/www.biovision.com\/media\/catalog\/product\/cache\/1\/image\/650x\/040ec09b1e35df139433887a97daa66f\/k\/8\/k808.jpg\" alt=\"\" width=\"568\" height=\"309\" \/><\/p>\n<p>\u4f9d\u7167\u8a66\u5291\u76d2\u65b9\u6848\uff0c\u4f7f\u7528\u4e0d\u540c\u91cf\u7684 PCA \u5c0d 400 \u00b5L \u8840\u6e05\u9032\u884c\u53bb\u86cb\u767d\u5316\u3002\u6e2c\u91cf\u6eb6\u6db2\u4e2d\u5269\u9918\u7684\u86cb\u767d\u8cea\u6fc3\u5ea6 OD280 (\u25c6) \u548c\u6a23\u54c1 pH (\u2022)\u3002\u5c07\u6a23\u672c\uff08\u7d14\u8840\u6e05\uff1b\u900f\u904e 10kD MW \u622a\u6b62\u503c\u904e\u6ffe\u7684\u8840\u6e05\uff1b\u548c PCA \u8655\u7406\uff09\u7a00\u91cb 20 \u500d\uff0c\u4ee5\u4fbf\u65bc\u6e2c\u91cf 280 nm \u7684 OD\u3002\u5c07\u76f8\u540c PCA \u8655\u7406\u7684\u6a23\u54c1\u7684\u55ae\u7368\u7b49\u5206\u90e8\u5206\u8207 pH 7.75 \u6e2c\u5b9a\u7de9\u885d\u6db2\u4ee5 1:1 \u6df7\u5408\uff0c\u4e26\u6e2c\u5b9a\u6df7\u5408\u7269\u7684 pH \u503c\u3002\u6b64\u5716\u986f\u793a\uff0c\u5728\u6240\u5f97\u6aa2\u6e2c pH \u503c\u4fdd\u6301\u5728\u76ee\u6a19\u6aa2\u6e2c pH \u503c\u7d04 0.5 \u500b pH \u55ae\u4f4d\u7684\u7bc4\u570d\u5167\u6642\uff0c\u53ef\u4ee5\u5bb9\u5fcd\u9ad8\u9054 20% \u7684\u6a23\u54c1\/PCA \u6bd4\u7387\u8aa4\u5dee\u3002\u4f7f\u7528 10 kDa MW \u622a\u6b62\u904e\u6ffe\u5668\u53ef\u53bb\u9664\u7d04 98% \u7684\u86cb\u767d\u8cea\uff0c\u800c PCA\/\u6a23\u672c\u6bd4\u4f8b\u70ba 1:4 \u6642\u7684 PCA \u6c89\u6fb1\u7269\u53ef\u53bb\u9664\u7d04 95% \u7684\u86cb\u767d\u8cea\u3002<\/p>\n<table width=\"838\">\n<tbody>\n<tr>\n<td><strong>Cat # +Size<\/strong><\/td>\n<td>K808-200 (ABCAM, ab284939)<\/td>\n<\/tr>\n<tr>\n<td><strong>Size<\/strong><\/td>\n<td>200 assays<\/td>\n<\/tr>\n<tr>\n<td><strong>Species Reactivity<\/strong><\/td>\n<td>Mammalian<\/td>\n<\/tr>\n<tr>\n<td><strong>Applications<\/strong><\/td>\n<td>Unique tool to prepare samples for the analysis of a variety of small molecules, proteins are precipitated, excess PCA is removed, samples are neutralized.<\/td>\n<\/tr>\n<tr>\n<td><strong>Features &amp; Benefits<\/strong><\/td>\n<td>\u2022 Samples prepared using this kit can be directly used in a wide variety of small molecule bioassays.<\/td>\n<\/tr>\n<tr>\n<td><strong>Kit Components<\/strong><\/td>\n<td>\u2022 PCA<br \/>\n\u2022 Neutralization Solution<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>\u86cb\u767d\u8cea\u5b9a\u91cf\u5546\u54c1 <a href=\"http:\/\/biopioneer.com.tw\/?p=9811\">Coomassie blue\u86cb\u767d\u8cea\u5b9a\u91cf\u5206\u6790\u5957\u7d44<\/a> | <a href=\"http:\/\/biopioneer.com.tw\/?p=10036\">BCA\u86cb\u767d\u6e2c\u5b9a\u8a66\u5291\u76d2(\u4f4e\u6fc3\u5ea610-600ug\/ml)| 5\uff05\u8010\u6d17\u6ecc <\/a>| <a href=\"http:\/\/biopioneer.com.tw\/?p=10023\">BCA\u86cb\u767d\u6e2c\u5b9a\u8a66\u5291\u76d2 (\u9084\u539f\u5291\u517c\u5bb9)<\/a><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u812b\u86cb\u767d\u6a23\u54c1\u88fd\u5099\u8a66\u5291\u76d2 | \u8ca8\u865fK808 (ABCAM, ab284939) Deproteinizing Sa [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":6512,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5261,5380,134],"tags":[3274],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13364"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=13364"}],"version-history":[{"count":6,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13364\/revisions"}],"predecessor-version":[{"id":45828,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13364\/revisions\/45828"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6512"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=13364"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=13364"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=13364"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}